Adipose/Fat - Tissue Tables

SpeciesSpecies DetailCellsEnzyme(s)Medium Reference
Avian Amyloid Fibrils Collagenase Type 3: 0.02%
Deoxyribonuclease I: 0.001%
 50mM Tris Scherpelz, K., Lu, J.,Tycko, R. and Meredith, S.: Preparation of Amyloid Fibrils Seeded from Brain and Meninges, Methods Mol Biol 1345, 299, 2016
Avian Amyloid Fibrils Collagenase Type 3: 0.02%
Deoxyribonuclease I: 0.001%
 50mM Tris Scherpelz, K., Lu, J.,Tycko, R. and Meredith, S.: Preparation of Amyloid Fibrils Seeded from Brain and Meninges, Methods Mol Biol 1345, 299, 2016
Bear Bear, Ursus arctos Adipocytes Collagenase Type 1: 0.1%
 HBSS Gehring, J., Rigano, K., Evans, H., Nelson, O., Robbins, C. and Jansen, H.: A Protocol for the Isolation and Cultivation of Brown Bear (Ursus arctos) Adipocytes., Cytotechnology 68, 2177-91, 2016
Bovine Bovine Adipocytes Collagenase Type 1: 40 u/ml
 Krebs-Ringer bicarbonate Yang, Y., Baldwin, R.: Preparation and Metabolism of Isolated Cells from Bovine Adipose Tissue, J Dairy Sci 56, 350, 1973
Canine 23-33 kg Stromal vascular Collagenase: see reference
 Media 199E Williams, S., Kosnik, P., Kleinert, L., Vossman, E., Lye, K. and Shine, M.: Adipose Stromal Vascular Fraction Cells Isolated Using an Automated Point of Care System Improve the Patency of Expanded Polytetrafluoroethylene Vascular Grafts., Tissue Eng Part A 19, 1295-302, 2013
Canine Canine Renal adipose derived cells Collagenase Type 1: 0.3%
 DMEM Basu, J., Genheimer, C., Sangha, N., Quinlan, S., Guthrie, K., Kelley, R., Ilagan, R., Jain, D., Bertram, T. and Ludlow, J.: Organ Specific Regenerative Markers in Peri-Organ Adipose: Kidney., Lipids Health Dis 10, 171, 2011
Canine Canine, 20-25 kg Adipose stem cells Collagenase: see reference
 Media-199 Fischer, L., McIlhenny, S., Tulenko, T., Golesorkhi, N., Zhang, P., Larson, R., Lombardi, J., Shapiro, I. and DiMuzio, P.: Endothelial Differentiation of Adipose-Derived Stem Cells: Effects of Endothelial Cell Growth Supplement and Shear Force., J Surg Res 152, 157, 2009
Canine Canine White fat Collagenase: 0.05%
 Kreb's Ringer bicarbonate buffer DiGirolamo, M., Mendlinger, S., and Fertig, J.W.: A Simple Method to Determine Fat Cell Size and Number in Four Mammalian Species, Am J Physiol 221, 850, 1971
Equine Equine Adipose derived stem cells Collagenase Type 1: 0.1%
 PBS Vidal, M., Robinson, S., Lopez, M., Paulsen, D., Borkhsenious, O., Johnson, J., Moore, R. and Gimble, J.: Comparison of Chondrogenic Potential in Equine Mesenchymal Stromal Cells Derived from Adipose Tissue and Bone Marrow., Vet Surg 37, 713, 2008
Equine Equine, 1-5 year Adipose derived stem cells Collagenase Type 1: 0.1%
 PBS Vidal, M., Kilroy, G., Lopez, M., Johnson, J., Moore, R. and Gimble, J.: Characterization of Equine Adipose Tissue-Derived Stromal Cells: Adipogenic and Osteogenic Capacity and Comparison with Bone Marrow-Derived Mesenchymal Stromal Cells., Vet Surg 36, 613, 2007
Fish Fish, Atlantic salmon Preadipocytes Collagenase Type 1: 0.1%
 HBSS Todorcevic, M., Vegusdal, A., Gjoen, T., Sundvold, H., Torstensen, B., Kjaer, M. and Ruyter, B.: Changes in Fatty Acids Metabolism During Differentiation of Atlantic Salmon Preadipocytes; Effects of n-3 and n-9 Fatty Acids., Biochim Biophys Acta 1781, 326, 2008
Gerbil Gerbil of unknown age
(also rat, hamster, rabbit, lamb, guinea-pig)		 Brown fat Collagenase Type 1: 0.10%
 Bicarbonate buffer Nedergaard, J. and Lindberg, O.: The Brown Fat Cell, Int Rev Cytol 74, 187, 1982
Guinea-Pig Guinea-pig, adult
(also rat, hamster, gerbil, rabbit, lamb)		 Brown fat Collagenase Type 1: 0.10%
 Bicarbonate buffer Nedergaard, J. and Lindberg, O.: The Brown Fat Cell, Int Rev Cytol 74, 187, 1982
Hamster Hamster, adult
(also rat, gerbil, rabbit, lamb, guinea-pig)		 Brown fat Collagenase Type 1: 0.10%
 Bicarbonate buffer Nedergaard, J. and Lindberg, O.: The Brown Fat Cell, Int Rev Cytol 74, 187, 1982
Hamster Hamster, 5 week-12 month White fat Collagenase: 0.05%
 Kreb's Ringer bicarbonate buffer DiGirolamo, M., Mendlinger, S., and Fertig, J.W.: A Simple Method to Determine Fat Cell Size and Number in Four Mammalian Species, Am J Physiol 221, 850, 1971
Human Human Adipose derived stem Collagenase Type 4: 150-300 u/ml
 RPMI-1640 Alstrup, T., Eijken, M., Bohn, A., Moller, B. and Damsgaard, T.: Isolation of Adipose Tissue-Derived Stem Cells: Enzymatic Digestion in Combination with Mechanical Distortion to Increase Adipose Tissue-Derived Stem Cell Yield from Human Aspirated Fat., Curr Protoc Stem Cell Biol 48, e68, 2019
Human Human Adipocytes Collagenase Type 2: 0.01-0.5%
 DMEM Tsurumachi, N., Akita, D., Kano, K., Matsumoto, T., Toriumi, T., Kazama, T., Oki, Y., Saito-Tamura, Y., Tonogi, M., Shimizu, N. and Honda, M.: Effect of Collagenase Concentration on The Isolation of Small Adipocytes from Human Buccal Fat Pad., J Oral Sci , , 2018
Human Human Adipocytes Collagenase Type 1: 0.1%
 HBSS Wahl, S., Drong, A., Lehne, B., Loh, M., Scott, K., Kunze, S. et al: Epigenome-wide Association Study of Body Mass Index, and the Adverse Outcomes of Adiposity, Nature 541, 81, 2017
Human Human Stromal Collagenase Type 2: 0.075%
 DMEM Duscher, D., Maan, Z., Luan, A., Aitzetmuller, M., Brett, E., Atashroo, D., Whittam, A., Hu, M., Walmsley, G., Houschyar, K., Schilling, A., Machens, H., Gurtner, G., Longaker, M. and Wan, D.: Ultrasound-Assisted Liposuction Provides a Source for Functional Adipose-Derived Stromal Cells., Cytotherapy 19, 1491-1500, 2017
Human Human Mesenchymal stromal Collagenase Type 1: 0.075%
 PBS Lin, Y., Marin-Argany, M., Dick, C., Redhage, K., Blancas-Mejia, L., Bulur, P., Butler, G., Deeds, M., Madden, B., Williams, A., Wall, J., Dietz, A. and Ramirez-Alvarado, M.: Mesenchymal Stromal Cells Protect Human Cardiomyocytes from Amyloid Fibril Damage, Cytotherapy 19, 1426-1437, 2017
Human Human Mesenchymal stem Collagenase Type 2: 0.2%
 DMEM/F-12 Munir, H., Ward, L., Sheriff, L., Kemble, S., Nayar, S., Barone, F., Nash, G. and McGettrick, H.: Adipogenic Differentiation of Mesenchymal Stem Cells Alters Their Immunomodulatory Properties in a Tissue-Specific Manner., Stem Cells 35, 1636-1646, 2017
Human Human Adipose derived mesenchymal stem Collagenase animal free: 0.1%
 PBS Clark, K., Fierro, F., Ko, E., Walker, N., Arzi, B., Tepper, C., Dahlenburg, H., Cicchetto, A., Koi, A., Marsh, L., Murphy, W., Fazel, N., and Borjesson, D.: Human and Feline Adipose-Derived Mesenchymal Stem Cells Have Comparable Phenotype, Immunomodulatory Functions, and Transcriptome., Stem Cell Res Ther 8, 69, 2017
Human Human Adipose derived endothelial Collagenase Type 1: 0.1%
 DMEM Szoke, K., Reinisch, A., Ostrup, E., Reinholt, F. and Brinchmann, J.: Autologous Cell Sources in Therapeutic Vasculogenesis: In Vitro and In Vivo Comparison of Endothelial Colony-Forming Cells from Peripheral Blood and Endothelial Cells Isolated from Adipose Tissue., Cytotherapy 18, 242-52, 2016
Human Human Adipose stem Collagenase Type 1: 0.1%
 DMEM/F12 Cheng, N., Hsieh, T., Lai, H. and Young, T.: High Glucose-Induced Reactive Oxygen Species Generation Promotes Stemness in Human Adipose-Derived Stem Cells, Cytotherapy 18, 371-83, 2016
Human Human Dental pulp stem Collagenase animal free: 0.1%
 HBSS Hu, J., Cao, Y., Xie, Y., Wang, H., Fan, Z., Wang, J., Zhang, C., Wang, J., Wu, C. aand Wang, S.: Periodontal Regeneration in Swine After Cell Injection and Cell Sheet Transplantation of Human Dental Pulp Stem Cells Following Good Manufacturing Practice., Stem Cell Res Ther 7, 130, 2016
Human Human Mesenchymal stromal Collagenase Type 4: 0.2%
 PBS Choudhery,M, Badowski, M, Muise, A and Harris, D: Effect of Mild Heat Stress on the Proliferative and Differentiative Ability of Human Mesenchymal Stromal Cells., Cytotherapy 17, 359-68, 2015
Human Human Adipose derived stem cells Collagenase Type 2: 0.1%
 HBSS Satish, L., Krill-Burger, J., Gallo, P., Etages, S., Liu, F., Philips, B., Ravuri, S., Marra, K., LaFramboise, W., Kathju, S. and Rubin, J.: Expression Analysis of Human Adipose-Derived Stem Cells During In Vitro Differentiation to an Adipocyte Lineage., BMC Med Genomics 8, 41, 2015
Human Human, female Adipose stromal Collagenase Type 1: 0.075%
 DMEM Kinoshita, K., Kuno, S., Ishimine, H., Aoi, N., Mineda, K., Kato, H., Doi, K., Kanayama, K., Feng, J., Mashiko, T., Kurisaki, A. and Yoshimura, K.: Therapeutic Potential of Adipose-Derived SSEA-3-Positive Muse Cells for Treating Diabetic Skin Ulcers., Stem Cells Transl Med 4, 146, 2015
Human Human Adipocytes Collagenase Type 1: 0.1%
 PBS Seaman, S., Tannan, S., Cao, Y., Peirce, S. and Lin, K.: Differential Effects of Processing Time and Duration of Collagenase Digestion on Human and Murine Fat Grafts., Plast Reconstr Surg 136, 189e-199e, 2015
Human Human Adipose stroma-vascular Neutral Protease: 2.4 u/ml
Collagenase: 250 u/ml
 PBS Esteve, D., Boulet, N., Volat, F., Zakaroff-Girard, A., Ledoux, S., Coupaye, M., Decaunes, P., Belles, C., Gaits-Iacovoni, F., Iacovoni, J., Remaury, A., Castel, B., Ferrara, P., Heymes, C., Lafonta, M. Boloumie, J.: Human White and Brite Adipogenesis is Supported by MSCA1 and is impaired by Immune Cells., Stem Cells 33, 1277-91, 2015
Human Human, adult Adipose derived mesenchymal stem Collagenase Type 2: 0.1%
 see reference Al-Saqi, S., Saliem, M., Asikainen, S., Quezada, H., Ekblad, A., Hovatta, O., Le Blanc, K., Jonasson, A. and Gotherstrom, C.: Defined Serum-Free Media for In Vitro Expansion of Adipose-Derived Mesenchymal Stem Cells., Cytotherapy 16, 915, 2014
Human Human, adult Adipose derived stem Collagenase Type 1: 0.15%
 DMEM Koellensperger, E., Bollinger, N., Dexheimer, V., Gramley, F., Germann, G. and Leimer, U.: Choosing the Right Type of Serum for Different Applications of Human Adipose Tissue-Derived Stem Cells: Influence on Proliferation and Differentiation Abilities., Cytotherapy 16, 789, 2014
Human Human Mesenchymal stromal Collagenase Type 1: 0.1%
 PBS Najar, M, Rodrigues, R, Buyl, K, Branson, S, Vanhaecke, T, Lagneaux, L, Rogiers, V and De Kock, J: Proliferative and Phenotypical Characteristics of Human Adipose Tissue-Derived Stem Cells: Comparison of Ficoll Gradient Centrifugation and Red Blood Cell Lysis Buffer Treatment Purification Methods., Cytotherapy 16, 1220-8, 2014
Human Human Adipose stromal stem Collagenase animal free: 200 u/ml
 DMEM/Hams F-12 Carvalho, P., Gimble, J., Dias, I., Gomes, M. and Reis, R.: Xenofree Enzymatic Products for the Isolation of Human Adipose-Derived Stromal/Stem Cells., Tiss Eng 19, 473-8, 2013
Human Human Stromal vascular fraction Collagenase Type 1: 0.075%
 PBS Doi, K., Tanaka, S., Iida, H., Eto, H., Kato, H., Aoi, N., Kuno, S., Hirohi, T. and Yoshimura, K.: Stromal Vascular Fraction Isolated from Lipo-Aspirates Using an Automated Processing System: Bench and Bed Analysis., J Tissue Eng Regen Med 7, 864, 2013
Human Human Adipose-derived stem Collagenase Type 1: 0.1%
 PBS Cervelli, V., Scioli, M., Gentile, P., Doldo, E., Bonanno, E., Spagnoli, L. and Orlandi, A.: Platelet-Rich Plasma Greatly Potentiates Insulin-Induced Adipogenic Differentiation of Human Adipose-Derived Stem Cells Through a Serine/Threonine Kinase Akt-dependent Mechanism and Promotes Clinical Fat Graft Maintenance., Stem Cells Transl Med 1, 206-20, 2012
Human Human Perivascular stem Collagenase Type 2: 0.1%
 DMEM James, A., Zara, J., Corselli, M., Askarinam, A., Zhou, A., Hourfar, A., Nguyen, A., Megerdichian, S., Asatrian, G., Pang, S., Stoker, D., Zhang, X., Wu, B., Ting, K., Peault, B. and Soo, C.: An Abundant Perivascular Source of Stem Cells for Bone Tissue Engineering., Stem Cells Transl Med 1, 673, 2012
Human Human Adipose derived stromal vascular Collagenase Type 1: 0.1%
 PBS Gentile, P., Orlandi, A., Scioli, M., Di Pasquali, C., Bocchini, I. and Cervelli, V.: Concise Review: Adipose-Derived Stromal Vascular Fraction Cells and Platelet-Rich Plasma: Basic and Clinical Implications for Tissue Engineering Therapies in Regenerative Surgery., Stem Cells Transl Med 1, 230-6, 2012
Human Human Stromal vascular, adipocytes Collagenase Type 1: 0.1%
 DMEM Gentile, P., Orlandi, A., Scioli, M., Di Pasquali, C., Bocchini, I., Curcio, C., Floris, M., Fiaschetti, V., Floris, R. and Cervell, V.: A Comparative Translational Study: The Combined Use of Enhanced Stromal Vascular Fraction and Platelet-Rich Plasma Improves Fat Grafting Maintenance in Breast Reconstruction., Stem Cells Transl Med 1, 341-51, 2012
Human Human, adult Stromovascular Collagenase Type 1: 0.1%
 PBS with BSA Hagman, D., Kuzma, J., Larson, I., Foster-Schubert, K., Kuan, L., Cignarella, A., Geamanu, E., Makar, K., Gottlieb, J. and Kratz, M.: Characterizing and Quantifying Leukocyte Populations in Human Adipose Tissue: Impact of Enzymatic Tissue Processing., J Immunol Methods 386, 50, 2012
Human Human Adipose derived stromal Collagenase Type 1: 0.1%
 PBS Naaijkens, B., Niessen, H., Prins, H., Krijnen, P., Kokhuis, T., de Jong, N,, van Hinsbergh, V., Kamp, O., Helder, M., Musters, R., van Dijk, A. and Juffermans, L.: Human Platelet Lysate as a Fetal Bovine Serum Substitute Improves Human Adipose-Derived Stromal Cell Culture for Future Cardiac Repair Applications., Cell Tissue Res 348, 119, 2012
Human Human Adipose derived stem Collagenase Type 1: 0.1%
 DMEM/F-12 Wu, I., Nahas, Z., Kimmerling, K., Rosson, G. and Elisseeff, J.: An Injectable Adipose Matrix for Soft-Tissue Reconstruction., Plast Reconstr Surg 129, 1247, 2012
Human Human, male 40-60 years Adipose derived stem cells Collagenase: 0.25%
Deoxyribonuclease I: 0.002%
 PBS Blasi, A., Martino, C., Balducci, L., Saldarelli, M., Soleti, A., Navone, S., Canzi, L., Cristini, S., Invernici, G., Parati, E. and Alessandri, G.: Dermal Fibroblasts Display Similar Phenotypic and Differentiation Capacity to Fat-Derived Mesenchymal Stem Cells, but Differ in Anti-Inflammatory and Angiogenic Potential, Vasc Cell 3, 5, 2011
Human Human Renal adipose derived cells Collagenase Type 1: 0.3%
 DMEM Basu, J., Genheimer, C., Sangha, N., Quinlan, S., Guthrie, K., Kelley, R., Ilagan, R., Jain, D., Bertram, T. and Ludlow, J.: Organ Specific Regenerative Markers in Peri-Organ Adipose: Kidney., Lipids Health Dis 10, 171, 2011
Human Human Adipose derived mesenchymal Collagenase Type 1: 0.1%
 L-DMEM Yang, X., He, X., He, J., Zhang, L., Su, X., Dong, Z., Xu, Y., Li, Y. and Li, YL.: High Efficient Isolation and Systematic Identification of Human Adipose-Derived Mesenchymal Stem Cells., J Biomed Sci 18, 59, 2011
Human Human Adipose derived stem Collagenase Type 1: 0.1%
 DMEM/Ham's F-12 Yu, G. Floyd, ZE., Wu, X., Halvorsen, Y. and Gimble, J.: Isolation of Human Adipose-Derived Stem Cells from Lipoaspirates., Methods Mol Biol 702, 17-27, 2011
Human Human Adipocytes Collagenase Type 1: 0.1%
 RPMI Basu, S., Haghiac, M., Surace, P., Challier, J., Guerre-Millo, M., Singh, K., Waters, T., Minium, J., Presley, L., Catalano, P. and Hauguel-de Mouzon, S.: Pregravid Obesity Associates with Increased Maternal Endotoxemia and Metabolic Inflammation., Obesity 19, 476, 2011
Human Human Adipocytes Collagenase Type 1: 0.1%
 KRB Peters, R., Wolf, M., van den Broek, M., Nuvolone, M., Dannenmann, S., Stieger, B., Rapold, R., Konrad, D., Rubin, A., Bertino, J., Aguzzi, A., Heikenwalder, M. and Knuth, A.: Efficient Generation of Multipotent Mesenchymal Stem Cells from Umbilical Cord Blood in Stroma-Free Liquid Culture., PLoS ONE 5, e15689, 2010
Human Human Adipose tissue-derived stem Collagenase Type 2: 1.0%
 DMEM/F12 Tan, H., DeFail, A., Rubin, J., Chu, C. and Marra, K.: Novel Multiarm PEG-Based Hydrogels for Tissue Engineering., J Biomed Mater Res 92, 979, 2010
Human Human Stromal vascular Collagenase Type 2: 0.1%
 HBSS Zimmerlin, L., Donnenberg, V., Pfeifer, M., Meyer, EM, Peault, B., Rubin, JP and Donnenberg, A.: Stromal Vascular Progenitors in Adult Human Adipose Tissue., Cytometry 77, 22-30, 2010
Human Human Adipose stromal Collagenase Type 1: 0.1%
 DMEM/Hams F-12 Yu, G., Wu, X., Dietrich, M., Polk, P., Scott, LK, Ptitsyn, A. and Gimble, J.: Yield and Characterization of Subcutaneous Human Adipose-Derived Stem Cells by Flow Cytometric and Adipogenic mRNA Analyzes., Cytotherapy 12, 538, 2010
Human Human Adipocytes Collagenase: 0.075%
 DMEM Suga, H., Eto, H., Shigeura, T., Inoue, K., Aoi, N., Kato, H., Nishimura, S., Manabe, I., Gonda, K. and Yoshimura, K.: IFATS Collection: Fibroblast Growth Factor-2-Induced Hepatocyte Growth Factor Secretion by Adipose-Derived Stromal Cells Inhibits Postinjury Fibrogenesis Through a c-Jun N-Terminal Kinase-Dependent Mechanism., Stem Cells 27, 238, 2009
Human Human Adipose stromal Collagenase Type 1: 0.1%
 DMEM Cai, L., Johnstone, B., Cook, T., Tan, J., Fishbein, M., Chen, P. and March, K.: IFATS Collection: Human Adipose Tissue-Derived Stem Cells Induce Angiogenesis and Nerve Sprouting Following Myocardial Infarction, in Conjunction with Potent Preservation of Cardiac Function., Stem Cells 27, 230, 2009
Human Human Adipose tissue-derived stem Collagenase Type 2: 1.0%
 DMEM/F12 Tan, H., Ramirez, C., Miljkovic, N., Li, H., Rubin, J. and Marra, K.: Thermosensitive Injectable Hyaluronic Acid Hydrogel for Adipose Tissue Engineering., Biomaterials 30, 6844, 2009
Human Human Adipose derived stem cells Collagenase Type 1: 0.1%
 DMEM Tandon, N., Goh, B., Marsano, A., Chao, P., Montouri-Sorrentino, C., Gimble, J. and Vunjak-Novakovic, G.: Alignment and Elongation of Human Adipose-Derived Stem Cells in Response to Direct-Current Electrical Stimulation., Conf Proc IEEE Eng Med Biol Soc 2009, 6517-21, 2009
Human Human Adipose derived stromal cells Collagenase Type 1: see reference
 DMEM Traktuev, D., Merfeld-Clauss, S., Li, J., Kolonin, M., Arap, W., Pasqualini, R., Johnstone, B., and March, K.: A Population of Multipotent CD34-Positive Adipose Stromal Cells Share Pericyte and Mesenchymal Surface Markers, Reside in a Periendothelial Location, and Stabilize Endothelial Networks, Circ Res 102, 77, 2008
Human Human Stromal Collagenase Type 1: 0.2%
 PBS Minana, M., Carbonell-Uberos, F., Mirabet, V., Marin, S. and Encabo, A.: IFATS Collection: Identification of Hemangioblasts in the Adult Human Adipose Tissue., Stem Cells 26, 2696, 2008
Human Human Stromal Collagenase Type 1: 0.1%
Neutral Protease:
DMEM Nie, J., Chang, B., Traktuev, D., Sun, J., March, K., Chan, L., Sage, H., Pasqualini, R., Arap, W. and Kolonin, M.: IFATS Collection: Combinatorial Peptides Identify alpha-5 beta-1 Integrin as a Receptor for the Matricellular Protein SPARC on Adipose Stromal Cells., Stem Cells 26, 2735, 2008
Human Human Stem Collagenase: 280 u/ml
 D-PBS Pilgaard, L., Lund, P., Rasmussen, J., Fink, T. and Zachar, V.: Comparative Analysis of Highly Defined Proteases for the Isolation of Adipose Tissue-Derived Stem Cells., Regen Med 3, 705-15, 2008
Human Human Adipocytes Collagenase Type 1: 0.2%
 HBSS Bujalska Iwona J, Durrani Omar M, Abbott Joseph, Onyimba Claire U, Khosla Pamela, Moosavi Areeb H, Reuser Tristan T Q, Stewart Paul M, Tomlinson Jeremy W, Walker Elizabeth A, Rauz Saaeha: Characterisation of 11beta-hydroxysteroid dehydrogenase 1 in human orbital adipose tissue: a comparison with subcutaneous and omental fat, J Endocrinol 192, 279-88, 2007
Human Human Adipose derived stromal cells Collagenase Type 1: 0.15%
 DMEM Schaffler A, Buchler C: Concise review: adipose tissue-derived stromal cells--basic and clinical implications for novel cell-based therapies, Stem Cells 25, 818-27, 2007
Human Human Adipose derived stem cells Collagenase Type 1: 0.1%
 DMEM/F12 Kilroy, G., Foster, S., Wu, X., Ruiz, J., Sherwood, S., Heifetz, A., Ludlow, J., Stricker, D., Potiny, S., Green, P., Halvorsen, Y., Cheatham, B., Storms, R. and Gimble, J.: Cytokine Profile of Human Adipose-Derived Stem Cells: Expression of Angiogenic, Hematopoietic, and Pro-Inflammatory Factors., J Cell Physiol 212, 702-9, 2007
Human Human Mesenchymal stem Collagenase Type 1: 0.1%
 HBSS Jeon Eun Su, Song Hae Young, Kim Mi Ra, Moon Hyun Jung, Bae Yong Chan, Jung Jin Sup, Kim Jae Ho: Sphingosylphosphorylcholine induces proliferation of human adipose tissue-derived mesenchymal stem cells via activation of JNK, J Lipid Res 47, 653-64, 2006
Human Human Preadipocytes Collagenase Type 1: 196 u/ml
 M199 Koellensperger, E., Von Heimburg, D., Markowicz, M. and Pallua, N.: Human Serum from Platelet-Poor Plasma for the Culture of Primary Human Preadipocytes., Stem Cells 24, 1218-25, 2006
Human Human Adipocytes, stromal vascular Collagenase: 0.2%
 HBSS Boquest Andrew C, Shahdadfar Aboulghassem, Fronsdal Katrine, Sigurjonsson Olafur, Tunheim Siv H, Collas Philippe, Brinchmann Jan E: Isolation and transcription profiling of purified uncultured human stromal stem cells: alteration of gene expression after in vitro cell culture, Mol Biol Cell 16, 1131-41, 2005
Human Human Multipotent adipose derived stem Collagenase: 0.2%
 DMEM Rodriguez Anne-Marie, Pisani Didier, Dechesne Claude A, Turc-Carel Claude, Kurzenne Jean-Yves, Wdziekonski Brigitte, Villageois Albert, Bagnis Claude, Breittmayer Jean-Philippe, Groux Herve, Ailhaud Gerard, Dani Christian: Transplantation of a multipotent cell population from human adipose tissue induces dystrophin expression in the immunocompetent mdx mouse, J Exp Med 201, 1397-405, 2005
Human Human Adult stem cells Collagenase Type 1: 0.1%
 PBS Devireddy, R., Thirumala, S. and Gimble, J.: Cellular Response of Adipose Derived Passage-4 Adult Stem Cells to Freezing Stress., J Biomech Eng 127, 1081, 2005
Human Human, adult, obese Adipocytes Collagenase Type 2: 0.1%
 DMEM/F12 Seboek, D., Linscheid, P., Zulewski, H., Langer, I., Christ-Crain, M., Keller, U., and Muller, B.: Somatostatin Is Expressed and Secreted by Human Adipose Tissue Upon Infection and Inflammation, J Clin Endocrinol Metab 89(10), 4833, 2004
Human Human, adult, female Preadipocytes Collagenase Type 1: 0.2%
 DMEM/F-12 Quickler, M., Sinha, B., Tomlinson, J., Bujalska, I., Stewart, P., Arlt, W.,: Androgen generation in adipose tissue in women with simple obesity--a site-specific role for 17beta-hydroxysteroid dehydrogenase type 5, J Endocrinol 183, 331, 2004
Human Human, adult Adipocytes Collagenase Type 1: 0.13%
 see reference Fain JN, Madan AK, Hiler ML, Cheema P, and Bahouth SW.: Comparison of the release of adipokines by adipose tissue, adipose tissue matrix, and adipocytes from visceral and subcutaneous abdominal adipose tissues of obese humans, Endocrinology 145, 2273, 2004
Human Human Stromal vascular, adipocytes Collagenase: 0.2%
 DMEM/F12 Planat-Benard Valerie, Silvestre Jean-Sebastien, Cousin Beatrice, Andre Mireille, Nibbelink Maryse, Tamarat Radia, Clergue Michel, Manneville Carole, Saillan-Barreau Corinne, Duriez Micheline, Tedgui Alain, Levy Bernard, Penicaud Luc, Casteilla Louis: Plasticity of human adipose lineage cells toward endothelial cells: physiological and therapeutic perspectives, Circulation 109, 656-63, 2004
Human Human Stromal vascular, adipocytes, stem Collagenase: 300 u/ml
 PBS Miranville A, Heeschen C, Sengenes C, Curat CA, Busse R, Bouloumie A: Improvement of postnatal neovascularization by human adipose tissue-derived stem cells, Circulation 110, 349-55, 2004
Human Human, adult, female, non-obese Adipocytes Collagenase Type 2: 0.05%
 DMEM Gesta, S., Lolmede, K., Daviaud, D., Berlan, M., Bouloumie, A., Lafontan, M., Valet, P., and Saulnier-Blache, J.: Culture of human adipose tissue explants leads to profound alteration of adipocyte gene expression, Horm Metab Res 35, 158, 2003
Human Human Adipocytes Collagenase Type 1: see reference
 Saline Patwardhan, R., Tubbs, R., Leonard, R., Kelly, D., Killingsworth, C., Rollins, D., Smith, W., Ideker, R., and Oakes, W.: Discernment of Adipose versus Nervous Tissue: A Novel Adjunct Solution in Lipomyelomeningocele Surgery, Ped Neurosurg 36, 314, 2002
Human Human, adult, male and female Adipocytes Collagenase Type 1: 0.2%
 DMEM/F12 McTernan, P., Anderson, L., Anwar, A., Eggo, M., Crocker, J., Barnett, A., Stewart, P., and Kumar, S.: Glucocorticoid Regulation of P450 Aromatase Activity in Human Adipose Tissue: Gender and Site Differences, J Clin Endocrinol Metab 87, 1327, 2002
Human Human Processed lipoaspirate cells Collagenase Type 1: 0.075%
 PBS Zuk, P., Zhu, M., Ashjian, P., De Ugarte, D., Huang, J., Mizuno, H., Alfonso, Z., Fraser, J., Benhaim, P., and Hedrick, M.: Human adipose tissue is a source of multipotent stem cells, Mol Biol Cell 13, 4279-95, 2002
Human Human Adipocytes Collagenase Type 1: 0.2%
 HBSS McTernan PG, Anwar A, Eggo MC, Barnett AH, Stewart PM, Kumar S: Gender differences in the regulation of P450 aromatase expression and activity in human adipose tissue, Int J Obes Relat Metab Disord 24, 875-81, 2000
Human Human, adult, male and female Adipocytes Collagenase Type 1: 0.1%
 DMEM/Ham's F-12 Gottschling-Zelle, H., Birgel, M., Scriba, D., Blum, W., and Hauner, H.: Depot-specific Release of Leptin from Subcutaneous and Omental Adipocytes in Suspension Culture: Effect of Tumor Necrosis Factor-alpha and Transforming Growth Factor-beta1, Eur J Endocrinol 141 (4), 436, 1999
Human Human, adult, male and female Adipocytes Collagenase Type 1: 0.1%
 DMEM/F12 Zhang, H., Kumar, S., Barnett, A., and Eggo, M.: Intrinsic Site-Specific Differences in the Expression of Leptin in Human Adipocytes and Its Autocrine Effects on Glucose Uptake, J Clin Endocrinol Metab 84, 2550, 1999
Human Human, non-diabetic, male Adipocytes Collagenase Type 1: 0.05%
 Kreb's Ringer bicarbonate buffer Anderson, O., Gliemann, J., and Gammeltoft: Receptor Binding and Biological Effect of Insulin in Human Adipocytes, Diabetologia 13, 589, 1977
Mouse Mouse Adipocytes Collagenase Type 1: 0.1%
 DMEM Jones, J., Rabhi, N., Orofino, J., Gamini, R., Perissi, V., Vernochet, C. and Farmer, S.: The Adipocyte Acquires a Fibroblast-Like Transcriptional Signature in Response to a High Fat Diet., Sci Rep 10, 2380, 2020
Mouse Mouse Adipose stromal Collagenase Type 1: 0.1%
 HBSS Bowles, A., Strong, A., Wise, R., Thomas, R., Gerstein, B., Dutreil, M., Hunter, R., Gimble, J. and Bunnell, B.: Adipose Stromal Vascular Fraction-Mediated Improvements at Late-Stage Disease in a Murine Model of Multiple Sclerosis., Stem Cells 35, 532-544, 2017
Mouse Mouse Adipose mesenchyal stromal Collagenase Type 2: 0.2%
 DMEM Maria, O., Shalaby, M., Syme, A., Eliopoulos, N. and Muanza, T.: Adipose Mesenchymal Stromal Cells Minimize and Repair Radiation-Induced Oral Mucositis., Cytotherapy 18, 1129-45, 2016
Mouse Mouse Adipose stromal Collagenase Type 1: 0.1%
 PBS Yao, W., Lay, Y., Kot, A., Liu, R., Zhang, H., Chen, H., Lam, K. and Lane, N.: Improved Mobilization of Exogenous Mesenchymal Stem Cells to Bone for Fracture Healing and Sex Difference., Stem Cells 34, 2587-2600, 2016
Mouse Mouse Adipocytes Collagenase: 0.1%
 Krebs Jang, H., Bhasin, S., Guarneri, T., Serra, C., Schneider, M., Lee, M., Guo, W., Fried, S., Pencina, K. and Jasuja, R.: The Effects of A Single Developmentally-Entrained Pulse of Testosterone in Female Neonatal Mice On Reproductive and Metabolic Functions in Adult Life., Endocrinology 156, 3737, 2015
Mouse Mouse Adipocytes Collagenase Type 1: 0.1%
 PBS Seaman, S., Tannan, S., Cao, Y., Peirce, S. and Lin, K.: Differential Effects of Processing Time and Duration of Collagenase Digestion on Human and Murine Fat Grafts., Plast Reconstr Surg 136, 189e-199e, 2015
Mouse Mouse, 20 week Stromal vascular Collagenase Type 2: 0.2%
 RPMI-1640 Kondo, T, Toyoshima, Y., Ishii, Y and Kyuwa, S.: Natural Killer T Cells in Adipose Tissue are Activated in Lean Mice., Exp Anim 62, 319, 2013
Mouse Mouse Adipose derived stem Collagenase Type 2: 0.1%
 DMEM Takahashi, H., Haraguchi, N., Nishikawa, S., Miyazaki, S., Suzuki, Y., Mizushima, T., Nishimura, J., Takemasa, I., Yamamoto, H., Mimori, K., Ishii, H., Doki, Y. and Mori, M.: Biological and Clinical Availability of Adipose-Derived Stem Cells for Pelvic Dead Space Repair., Stem Cells Transl Med 1, 803, 2012
Mouse Mouse Stem and progenitor Collagenase Type 2: 0.2%
 HBSS Han, J., Koh, Y., Moon, H., Ryoo, H., Cho, C., Kim, I. and Koh, G.: Adipose Tissue is an Extramedullary Reservoir for Functional Hematopoietic Stem and Progenitor Cells., Blood 115, 957, 2010
Mouse Mouse, 3 week Adipocytes Collagenase Type 1: 0.2%
 HBSS De Matteis, R., Zingaretti, M., Murano, I., Vitali, A., Frontini, A., Giannulis, I., Barbatelli, G., Marcucci, F., Bordicchia, M., Sarzani, R., Raviola, E. and Cinti, S.: In Vivo Physiological Transdifferentiation of Adult Adipose Cells., Stem Cells 27, 2761, 2009
Mouse Mouse White adipocytes Collagenase Type 2: 0.1%
 DMEM Wong, K., Szeto, F, Zhang, W., Ye, H., Kong, J., Zhang, Z., Sun, X. and Li, Y: Involvement of the Vitamin D Receptor in Energy Metabolism: Regulation of Uncoupling Proteins., Am J Physiol/Endo 296, 820, 2009
Mouse Mouse Adipose stromal Collagenase Type 2: 0.075%
 DMEM Thangarajah, H., Vial, I., Chang, E., El-Ftesi, S., Januszyk, M., Chang, E., Paterno, J., Neofytou, E., Longaker, M. and Gurtner, G.: IFATS Collection: Adipose Stromal Cells Adopt a Proangiogenic Phenotype Under the Influence of Hypoxia., Stem Cells 27, 266, 2009
Mouse Mouse Adipose tissue-derived stem Collagenase Type 1: 0.075%
 Modified Eagles Cho, K., Park, H., Park, H., Jung, J., Jeon, S., Kim, Y. and Roh, H.: IFATS Collection: Immunomodulatory Effects of Adipose Tissue-Derived Stem Cells in an Allergic Rhinitis Mouse Model., Stem Cells 27, 259-65, 2009
Mouse Mouse, C57Bl/6J Adipocytes Collagenase Type 1: 0.15%
 DMEM/F12 Aoyagi T, Shimba S, Tezuka M: Characteristics of Circadian Gene Expressions in Mice White Adipose Tissue and 3T3-L1 Adipocytes, J Health Sci 51, 21, 2005
Mouse Mouse, 3-6 day and 2-3 month Adipose-derived stromal cells Collagenase Type 2: 0.075%
 PBS Cowan Catherine M, Shi Yun-Ying, Aalami Oliver O, Chou Yu-Fen, Mari Carina, Thomas Romy, Quarto Natalina, Contag Christopher H, Wu Benjamin, Longaker Michael T: Adipose-derived adult stromal cells heal critical-size mouse calvarial defects, Nat Biotechnol 22, 560-7, 2004
Mouse Mouse Stromal vascular, adipocytes Collagenase: 0.2%
 DMEM/F12 Planat-Benard Valerie, Silvestre Jean-Sebastien, Cousin Beatrice, Andre Mireille, Nibbelink Maryse, Tamarat Radia, Clergue Michel, Manneville Carole, Saillan-Barreau Corinne, Duriez Micheline, Tedgui Alain, Levy Bernard, Penicaud Luc, Casteilla Louis: Plasticity of human adipose lineage cells toward endothelial cells: physiological and therapeutic perspectives, Circulation 109, 656-63, 2004
Mouse Mouse, C57BL/6J and FVB, male, 8-9 week Adipocytes Collagenase: see reference
 DMEM Ruan, H., Zarnowski, MJ., Cushman, S., and Lodish, H.: Standared Isolation of Primary Adipose Cells from Mouse Epididymal Fat Pads Induces Inflammatory Mediators and Down-regulates Adipocyte Genes, J Biol Chem 278, 47585, 2003
Mouse Mouse, both sexes Adipocytes Collagenase: 0.05%
 Krebs-Ringer Phosphate HEPES (KRPH) Nadler, S., Stoehr, J., Rabaglia, M., Schueler, K., Birnbaum, M., and Attie, A.: Normal Akt/PKB with Reduced PI3K Activation in Insulin-resistant Mice, Am J Physiol/Endo 281, E1249, 2001
Mouse Mouse, B6D2F1, F1 hybrids, New Zealand black female & New Zealand white male Vascular endothelial Collagenase: 0.2%
 PBS Launder, T., Gegen, N., Knedler, A., and Harbeck, R.: The Isolation and Characterization of Enriched Microvascular Endothelial Cells From Mouse Adipose Tissue, J Immunol Methods 102, 45, 1987
Porcine Porcine, female, <1 year Adipose mesenchymal stem Collagenase Type 1: 0.1%
 DMEM Williams, K., Picou, A., Kish, S., Giraldo, A., Godke, R. and Bondioli, K: Isolation and Characterization of Porcine Adipose Tissue-Derived Adult Stem Cells., Cells Tissues Organs 188, 251, 2008
Porcine Porcine, crossbred, male 1-4 day Adipocytes Collagenase Type 1: 0.2%
 DMEM/F12 Ramsay, T.G.: Porcine Leptin Inhibits Lipogenesis in Porcine Adipocytes, J Anim Sci 81, 3008, 2003
Porcine Porcine, crossbred Adipocytes Collagenase Type 1: 0.067%
 Krebs-Ringer Liang, W. and Mills, S.: Quantitative Analysis of beta-Adrenergic Receptor Subtypes in Pig Tissues., J Anim Sci 80, 963-70, 2002
Porcine Porcine, 8-9 week Adipose Collagenase Type 1: 300 u/ml
 HEPES Ding, S., McNeel, R., and Mersmann, H.: Expression of Porcine Adipocyte Transcripts: Tissue Distribution and Differentiation In Vitro and In Vivo, Comp Biochem Physiol B 123, 307, 1999
Porcine Porcine, neonatal Adipocytes Collagenase Type 1: 0.3%
 Krebs-Ringer bicarbonate albumin Wang Y, Fried SK, Petersen RN, Schoknecht PA: Somatotropin regulates adipose tissue metabolism in neonatal swine, J Nutr 129, 139-45, 1999
Porcine Porcine, crossbred, 1-3 day Adipose, Stromal-vascular Collagenase Type 1: 0.2%
 DMEM/F12 Suryawan, A., Swanson, L., and Hu, C.: Insulin and Hydrocortisone, But Not Triiodothyronine, Are Required for the Differentiation of Pig Preadipocytes in Primary Culture, J Anim Sci 75, 105, 1997
Rat Rat, SD, 2-3 month Mesenchymal stromal Collagenase Type 1: 0.1%
 DMEM Dayer, D., Tabar, M., Moghimipour, E., Tabandeh, M., Ghadiri, A., Bakhshi, E., Orazizadeh, M. and Ghafari, M.: Sonic Hedgehog Pathway Suppression and Reactivation Accelerates Differentiation of Rat Adipose-Derived Mesenchymal Stromal Cells Toward Insulin-Producing Cells, Cytotherapy 19, 937-946, 2017
Rat Rat, 9 week Adipose stromal Collagenase: 0.1%
 DMEM Ohta, Y., Hamaguchi, A., Ootaki, M., Watanabe, M., Takeba, Y., Iiri, T., Matsumoto, N. and Takenaga, M.: Intravenous Infusion of Adipose-Derived Stem/Stromal Cells Improves Functional Recovery of Rats with Spinal Cord Injury., Cytotherapy 19, 839-848, 2017
Rat Rat Mesenchymal stem Collagenase Type 1: 0.075%
 DMEM Emre, E, Yuksel, N, Duruksu, G, Pirhan, D, Subasi, C, Erman, G and Karaoz, E: Neuroprotective Effects of Intravitreally Transplanted Adipose Tissue and Bone Marrow-Derived Mesenchymal Stem Cells in an Experimental Ocular Hypertension Model., Cytotherapy 17, 543-59, 2015
Rat Rat, SD, 5 month Mesenchymal stromal Collagenase Type 2: 0.075%
 DMEM Veronesi, F, Torricelli, P, Della, B, Pagani, S and Fini, M: In Vitro Mutual Interaction Between Tenocytes and Adipose-Derived Mesenchymal Stromal Cells., Cytotherapy 17, 215-23, 2015
Rat Rat Adipocytes Collagenase Type 1: 0.3%
 Krebs-Ringer Ogasawara, J., Izawa, T., Sakurai, T., Shirato, K., Ishibashi, Y., Ohira, Y., Ishida, H., Ohno, H. and Kizaki, T.: Habitual Exercise Training Acts as a Physiological Stimulator for Constant Activation of Lipolytic Enzymes in Rat Primary White Adipocytes., Biochem Biophys Res Commun 464, 348-53, 2015
Rat Rat, adult Stromal vascular Collagenase Type 1: 0.2%
 PBS Leblanc, A., Nguyen, Q., Touroo, J., Aird, A., Chang, R., Ng, C., Hoying, J. and Williams, S.: Adipose-derived Cell Construct Stabilizes Heart Function and Increases Microvascular Perfusion in an Established Infarct., Stem Cells Transl Med 2, 896-905, 2013
Rat Rat, male, 300g Adipocytes Collagenase Type 2: 0.33%
 RPMI 1640 Thompson, A., Nunez, M., Davidson, R., Horm, T., Schnittker, K., Hart, M., Suarez, A. and Tsao, T.: Mitigation of Isolation-Associated Adipocyte Interleukin-6 Secretion Following Rapid Dissociation of Adipose Tissue., J Lipid Res 53, 2797, 2012
Rat Rat, Lewis, male Renal adipose derived cells Collagenase Type 1: 0.3%
 DMEM Basu, J., Genheimer, C., Sangha, N., Quinlan, S., Guthrie, K., Kelley, R., Ilagan, R., Jain, D., Bertram, T. and Ludlow, J.: Organ Specific Regenerative Markers in Peri-Organ Adipose: Kidney., Lipids Health Dis 10, 171, 2011
Rat Rat, SD, neonatal Brown adipocytes Collagenase Type 4: 0.1%
Neutral Protease: 0.1%
Trypsin: 0.05%
 PBS Liu, Z., Wang, H., Zhang, Y., Zhou, J., Lin, Q., Wang, Y., Duan, C., Wu, K. and Wang, C.: Efficient Isolation of Cardiac Stem Cells from Brown Adipose., J Biomed Biotechnol 2010, 104296, 2010
Rat Rat Adipose derived stem Collagenase Type 1: 0.1%
 DMEM Wei, X., Du, Z., Zhao, L., Feng, D., Wei, G., He, Y., Tan, J., Lee, W., Hampel, H., Dodel, R., Johnstone, B., March, K., Farlow, M. and Du, Y.: IFATS Collection: The Conditioned Media of Adipose Stromal Cells Protect Against Hypoxia-Ischemia-Induced Brain Damage In Neonatal Rats., Stem Cells 27, 478, 2009
Rat Rat Adipose tissue-derived stem Collagenase Type 2: 0.075%
 DMEM Tomiyama, K., Murase, N., Stolz, D., Toyokawa, H., O'Donnell, D., Smith, D., Dudas, J., Rubin, J. and Marra, K.: Characterization of Transplanted Green Fluorescent Protein+ Bone Marrow Cells Into Adipose Tissue., Stem Cells 26, 330, 2008
Rat Rat, Wistar, 4 week Adipocytes Collagenase: 0.2%
 Ham's F12 Aoki, S., Toda, S., Sakemi, T., and Sugihara, H.: Coculture of Endothelial Cells and Mature Adipocytes Actively Promotes Immature Preadipocyte Development In Vitro, Cell Struct Funct 28, 55, 2003
Rat Rat, SD Adipocytes Collagenase Type 1: 0.2%
 KRHB Mora, S., Yang, C., Ryder, J., Boeglin, D and Pessin, J: The MEF2A and MEF2D Isoforms are Differentially Regulated in Muscle and Adipose Tissue during States of Insulin Deficiency, Endocrinology 142, 1999, 2001
Rat Rat, SD, male, 4-7 weeks Brown adipocytes Deoxyribonuclease I: 0.5%
 DMEM Omatsu-Kanbe, M., and Matsuura, H.: Inhibition of Store-operated Ca2+ Entry by Extracellular ATP in Rat Brown Adipocytes, J Physiol 521 (3), 601, 1999
Rat Rat Adipocytes Collagenase Type 2: 0.2%
 DMEM /F-12 Serrero, G: Primary Culture in Defined Medium of Adipocyte Precursors,Cell & Tissue Culture: Laboratory Procedures Vol. 1,Doyle, A., Griffiths, J., and Newell, D., John Wiley and Sons, Ltd., 11B:6.1, 1995
Rat Rat, SD, male, 130-160 g Adipose
Epididymal fat pads		 Collagenase: 0.3%
 Kreb's-Ringer bicarbonate buffer modified Charron, M.J. and Kahn, B.B.: Divergent Molecular Mechanisms for Insulin-Resistant Glucose Transport in Muscle and Adipose Cells In Vivo, J Biol Chem 265, 7994, 1990
Rat Rat, 3 day Preadipocytes Collagenase Type 3: 0.10%
 Parker Medium 199 Gaben-Cogneville, A., Poussin, B., Chamblier, M., Forgue-Fafitte, M., and Rosselin, G.: Development of Insulin and Epidermal Growth Factor Receptors During the Differentiation of Rat Preadipocytes in Primary Culture, Biochim Biophys Acta 968, 231, 1988
Rat Rat, SD, various weights and ages Brown adipocytes
Interscapular & cervical depots		 Collagenase: 0.2%
Soybean Trypsin Inhibitor: 0.3%
 Krebs Ringer bicarbonate buffer Woodward, Julie A. and Saggerson, E.: Effect of Adenosine Deaminase, N6-Phenylisopropyladenosine and Hypothroidism on the Responsiveness of Rat Brown Adipocytes to Noradrenaline, Biochem J 238, 395, 1986
Rat Rat, CD, male, 150-200 g Adipocytes
Epididymal fat pads		 Collagenase: 0.1%
 Krebs Ringer bicarbonate buffer Pessin, J.E., Gitomer, W., Oka, Y., Oppenheimer, C.L., and Czech, M.P.: ß-Adrenergic Regulation of Insulin and Epidermal Growth Factor Receptors in Rat Adipocytes, J Biol Chem 258, 7386, 1983
Rat Rat, Wistar, albino, male, 100-140 g Adipocytes
Epididymal-fat pads		 Collagenase: 0.3%
 Kreb's Ringer Green, A. and Newsholme, E.: Sensitivity of Glucose Uptake and Lipolysis of White Adipocytes of the Rat to Insulin and Effects of Some Metabolites, Biochem J 180, 365, 1979
Rat Rat, Fischer, 344, male, 9 - 13 week White fat Collagenase: 0.3%
 Kreb's Ringer bicarbonate buffer Stiles, J.W., Francendese, A.A. and Masoro, E.J.: Influence of Age on Size and Number of Fat Cells in the Epididymal Depot, Am J Physiol 229, 1561, 1975
Rat Rat, Wistar, male, 5 week-16 month White fat Collagenase: 0.05%
 Kreb's Ringer bicarbonate buffer DiGirolamo, M., Mendlinger, S., and Fertig, J.W.: A Simple Method to Determine Fat Cell Size and Number in Four Mammalian Species, Am J Physiol 221, 850, 1971
Rat Rat (CFE), albino, female Brown fat Collagenase Type 1: 0.10%
 Bicarbonate buffer Fain, J., Reed, N., and Saperstein, R.: Isolation and Metabolism of Brown Fat Cells, J Biol Chem 242, 1887, 1967
Rat Rat, SD, male, 160-210 g Fat Collagenase: 0.3%
 Albumin-bicarbonate buffer Rodbell, M.: Metabolism of Isolated Fat Cells. I. Effects of Hormones on Glucose Metabolism and Lipolysis, J Biol Chem 239 (2), 375, 1964
Squirrel Squirrel Brown adipocytes Collagenase Type 2: 0.16%
 Krebs-Ringer phosphate McFarlane, S., Mathers, K. and Staples, J.: Reversible Temperature-Dependent Differences in Brown Adipose Tissue Respiration During Torpor in a Mammalian Hibernator., Am J Physiol Regul Integr Comp Physiol 312, R434-R442, 2017