Adrenal - Tissue Tables
Species | Species Detail | Cells | Enzyme(s) | Medium |
Reference |
Bovine
|
Bovine |
Chromaffin |
Collagenase Type 1: 0.1-0.2%
Deoxyribonuclease I: 0.003-0.015%
Hyaluronidase: 0.015%
|
DMEM/F12 |
Dominguez, N., Rodriguez, M., Machado, J. and Borges, R.: Preparation and Culture of Adrenal Chromaffin Cells., Methods Mol Biol 846,
223, 2012 |
Bovine
|
Bovine, 6 month |
Chromaffin |
Collagenase Type 1: 0.125%
|
Locke's solution |
Moustafa T, Girod S, Tortosa F, Li R, Sol JC, Rodriguez F, Bastide R, Lazorthes Y, Sallerin B: Viability and functionality of bovine chromaffin cells encapsulated into alginate-PLL microcapsules with a liquefied inner core, Cell Transplant 15,
121-33, 2006 |
Bovine
|
Bovine |
Chromaffin cells |
Collagenase: 0.1%
Deoxyribonuclease I: 30 u/ml
|
DMEM |
Hahm, S., Chen, Y., Vinson, C. and Eiden, L.: A Calcium-Initiated Signaling Pathway Propagated Through Calcineurin and cAMP Response Element-Binding Protein Activates Proenkephalin Gene Transcription after Depolarization., Mol Pharmacol 64,
1503, 2003 |
Bovine
|
Bovine |
Chromaffin |
Collagenase: 0.2%
|
Locke's solution |
Ortega, J., Sagen, J., and Pappas, G.: Short-term Immunosuppression Enhances Long-term Survival of Bovine Chromaffin Cell Xenografts in Rat CNS, Cell Transplant 1,
33, 1992 |
Bovine
|
Bovine |
Chromaffin |
Deoxyribonuclease I: 30 u/mg
|
HEPES |
Zhu, J., Li, W., Toews, M., and Hexum, T.: Neuropeptide Y Inhibits Forskolin-Stimulated Adenylate Cyclase in Bovine Adrenal Chromaffin Cells via a Pertussis Toxin-Sensitive Process, J Pharmacol Exp Ther 263 (3),
1479, 1992 |
Bovine
|
Bovine (also rat) |
Heart Adrenal chromaffin Paraneurons |
Trypsin: 0.06%
|
25mM HEPES buffered Locke's solution, CMF |
Trifaro, J., Tang, R., and Novas, M.: Monolayer Co-Culture of Rat Heart Cells and Bovine Adrenal Chromaffin Paraneurons, In Vitro Cell Dev Biol 26,
335, 1990 |
Bovine
|
Bovine |
Chromaffin |
Collagenase Type 1: 0.25 %
|
DMEM |
Dahmer, M., Hart, P., and Perlman, R.: Studies on the Effect of Insulin-Like Growth Factor-I on Catecholamine Secretion from Chromaffin Cells, J Neurochem 54 (3),
931, 1990 |
Bovine
|
Bovine |
Chromaffin |
Collagenase: 0.2%
Deoxyribonuclease I: 0.015%
|
Locke's solution |
Higgins, L., and Berg, D.: Immunological Identification of a Nicotinic Acetylcholine Receptor on Bovine Chromaffin Cells, J Neurosci 7 (6),
1792, 1987 |
Bovine
|
Bovine |
Chromaffin |
Collagenase: 0.05%
|
Locke's solution, CMF |
Aunis, D., Rotllan, P., and Miras-Portugal, M.: Incorporation of Adenosine into Nucleotides of Chromaffin Cells in Culture, Neurochem Int 7,
89, 1985 |
Bovine
|
Bovine |
Chromaffin |
Collagenase: 0.15%
|
Kreb's, CMF |
Almazan, G., Aunis, D., Garcia, A., Montiel, C., Nicolas, G., and Sanchez-Garcia, P.: Effects Of CLS on the Release of Noradrenaline From Chromaffin Cells, Br J Biomed Sci 81,
599, 1984 |
Bovine
|
Bovine |
Chromaffin |
Collagenase: 0.1%
|
(see reference) |
Pollard, H., Pazoles, C., Creutz, C., Scott, J., Zinder, O., and Hotchkiss, A.: An Osmotic Mechanism For Exocytosis From Dissociated Chromaffin Cells, J Biol Chem 259,
1114, 1984 |
Bovine
|
Bovine |
Chromaffin |
Collagenase Type 1: 0.05%
|
CF Kreb's |
Cena, V., Garcia, A., Montiel, C., and Sanchez-Garcia, P.: Uptake of [3H]-nicotine and [3H]-noradrenaline by Cultured Chromaffin Cells, Br J Pharmacol 81,
119, 1984 |
Bovine
|
Bovine |
Chromaffin |
Collagenase Type 1: 0.025%
|
HBSS, modified |
Waymire, J., Bennett, W., Boehme, R., Hankins, L., Gilmer-Waymire, K., and Haycock, J.: Bovine Adrenal Chromaffin Cells: High-Yield Purification and Viability in Suspension Culture, J Neurosci Methods 7,
329, 1983 |
Bovine
|
Bovine |
Medulla |
Hyaluronidase: 0.2%
|
Saline w/BSA 0.5% |
Knight, D. and Baker, P.: Stimulus-Secretion Coupling in Isolated Bovine Adrenal Medullary Cells, Q J Exp Physiol 68,
123, 1983 |
Bovine
|
Bovine |
Medulla |
Collagenase: 0.2%
|
Krebs-Ringer bicarbonate buffer, CMF |
Greenberg, A. and Zinder, O.: alpha- and beta-Receptor Control of Catecholamine Secretion from Isolated Adrenal Medulla Cells, Cell Tissue Res 226,
655, 1982 |
Bovine
|
Bovine |
Chromaffin |
Deoxyribonuclease I: 15 µg/ml
|
Medium A (see reference) |
Wilson, S.P., and Viveros, O.H.: Primary Culture of Adrenal Medullary Chromaffin Cells in a Chemically Defined Medium, Exp Cell Res
133,
159, 1981 |
Bovine
|
Bovine |
Medulla |
Protease: 0.2%
|
Saline |
Baker, P., and Knight., D: Calcium Control of Exocytosis and Endocytosis in Bovine Adrenal Medullary Cells, Phil Trans R Soc Lond 296,
83, 1981 |
Bovine
|
Bovine |
Chromaffin |
Collagenase: 0.05%
|
Locke's solution, CMF |
Trifaro, J.M., and Lee, R.W.: Morphological Characteristics and Stimulus-Secretion Coupling in Bovine Adrenal Chromaffin Cell Cultures, Neuroscience 5,
1533, 1980 |
Bovine
|
Bovine, adult (also rat, Hanover-Wistar, young; guinea-pig, newborn) |
Chromaffin |
Collagenase: 0.5%
Collagenase: 0.5%
|
HBSS |
Unsicker, K., Rieffert, B., and Ziegler, W.: Effects of Cell Culture Conditions, Nerve Growth Factor, Dexamethasone, and Cyclic AMP on Adrenal Chromaffin Cells In Vitro, Adv Biochem Psychopharmacol 255,
51, 1980 |
Bovine
|
Bovine |
Chromaffin |
Collagenase: 0.25%
|
F-12 medium |
Kumakura, K., Karoum, F., Guidotti, A., and Costa, E.: Modulation of Nicotinic Receptors by Opiate Receptor Agonists in Cultured Adrenal Chromaffin Cells, Nature
283,
489, 1980 |
Bovine
|
Bovine |
Medulla |
Collagenase: 0.05%
|
Locke's solution, CF |
Kilpatrick, D., Ledbetter, F., Carson, K., Kirshner, A., Slepetis, R., and Kirshner, N.: Stability of Bovine Adrenal Medulla Cells in Culture, J Neurochem 35 (3),
679, 1980 |
Bovine
|
Bovine, adult |
Medulla |
Collagenase Type 1: 0.5%
|
HBSS |
Unsicker, K., and Griesser, G.: Establishment, Characterization and Fibre Outgrowth of Isolated Bovine Adrenal Medullary Cells in Long-Term Cultures, Neuroscience 5,
1445, 1980 |
Bovine
|
Bovine |
Chromaffin |
Collagenase: 0.05%
|
DMEM |
Aunis, D., Guerold, B., Bader, M-F., and Cieselski-Treska, J.: Immunocytochemical and Biochemical Demonstration of Contractile Proteins in Chromaffin Cells, Neuroscience 5,
2261, 1980 |
Bovine
|
Bovine |
Medullary |
Collagenase Type 2: 0.2%
|
HEPES |
Hersey, R., and DiStefano, V.: Control of Phenylethanolamine N-Methyltransferase by Glucocorticoids in Cultured Bovine Adrenal Medullary Cells, J Pharmacol Exp Ther 209 (1),
147, 1979 |
Bovine
|
Bovine |
Foreskin |
Collagenase: 0.5%
|
Dulbecco's MEM w/10% calf serum |
Folkman, J., Haudenschild, C. C., and Zetter, B. R.: Long-term Culture of Capillary Endothlial Cells, Proc Natl Acad Sci U S A 76,
5217, 1979 |
Bovine
|
Bovine |
Medulla |
Collagenase Type 1: 0.05%
|
Kreb's, CF |
Fenwick, E., Fajdiga, P., Howe, N., and Livett, B.: Functional and Morphological Characterization of Isolated Bovine Adrenal Medullary Cell, J Cell Biol
76,
12, 1978 |
Bovine
|
Bovine |
Chromaffin |
Hyaluronidase: 0.2%
|
HEPES, CF |
Brooks, J.C.: The Isolated Bovine Adrenomedullary Chromaffin Cell: A Model of Neuronal Excitation-Secretion, Endocrinology 101,
1369, 1977 |
Bovine
|
Bovine, male |
Chromaffin |
Collagenase Type 1: 0.2%
|
Kreb's Ringer bicarbonate buffer, CF |
Schneider, A., Herz, R., and Rosenheck, K.: Stimulus-Secretion Coupling in Chromaffin Isolated From Adrenal Medulla, Proc Natl Acad Sci U S A 74,
5036, 1977 |
Guinea-Pig
|
Guinea-pig, 500-700 g |
Chromaffin Medulla |
Collagenase:
|
BSS (see reference) |
Role, L.W., Leeman, S.E., and Perlman, R.L.: Somatostain and Substance P Inhibit Catecholamine Secretion from Isolated Cells of Guinea-pig Adrenal Medulla, Neurochem Int 6,
1813, 1981 |
Guinea-Pig
|
Guinea-pig (also rat, Hanover-Wistar, young; newborn; cattle) |
Chromaffin |
Collagenase: 0.5%
Collagenase: 0.5%
|
HBSS |
Unsicker, K., Rieffert, B., and Ziegler, W.: Effects of Cell Culture Conditions, Nerve Growth Factor, Dexamethasone, and Cyclic AMP on Adrenal Chromaffin Cells In Vitro, Adv Biochem Psychopharmacol 255,
51, 1980 |
Guinea-Pig
|
Guinea-pig |
Adrenal Chromaffin |
Collagenase: 0.05%-0.20%
|
Kreb's-Ringer bicarb glucose buffer, CF |
Hochman, J., and Perlman, R.L.: Catecholamine Secretion by Isolated Adrenal Cells, Biochim Biophys Acta 421,
168, 1976 |
Hamster
|
Hamster (Mesocricetus auratus) 100-150 g |
Adrenal Chromaffin |
Hyaluronidase: 0.20%
|
Kreb's Ringer bicarbonate buffer |
Liang, B.T., and Perlman, R.L.: Catecholamine Secretion by Hamster Adrenal Cells, J Neurochem 32,
927, 1979 |
Human
|
Human |
Adrenal medulla progenitor |
Collagenase: 0.2%
|
DMEM/F-12 |
Santana, M., Chung, K., Vukicevic, V., Rosmaninho-Salgado, J., Kanczkowski, W., Cortez, V., Hackmann, K., Bastos, C., Mota, A., Schrock, E., Bornstein, S., Cavadas, C. and Ehrhart-Bornstein, M.: Isolation, Characterization, and Differentiation of Progenitor Cells from Human Adult Adrenal Medulla., Stem Cells Transl Med 1,
783, 2012 |
Human
|
Human |
Chromaffin cells |
Collagenase: 0.2%
|
Locke's solution |
Jeon, Y., Baek, W., Chung, S., Shin, N., Kim, H., and Lee, S.: Cultured Human Chromaffin Cells Grafted in Spinal Subarachnoid Space Relieves Allodynia in a Pain Rat Model., Korean J Anesthesiol
60,
357, 2011 |
Human
|
Human |
Adrenocortical |
Collagenase Type 1: 0.2%
Deoxyribonuclease I: 0.01%
|
Krebs Ringer |
Caroccia, B., Fassina, A., Seccia, T., Recarti, C., Petrelli, L., Belloni, A., Pelizzo, M. and Rossi, G.: Isolation of Human Adrenocortical Aldosterone-Producing Cells by a Novel Immunomagnetic Beads Method., Endocrinology 151,
1375, 2010 |
Human
|
Human, adult |
Chromaffin |
Trypsin: 0.25%
|
Eagle's MEM |
Tischler, A., DeLellis, R., Bailes, B., Nunnemacher, G.,Carabba, V., and Wolfe, H.: Nerve Growth Factor-Induced Neurite Outgrowth from Normal Human Chromaffin Cells, Lab Invest 43,
399, 1980 |
Human
|
Human, adult and child |
Foreskin |
Collagenase: 0.5%
|
Dulbecco's MEM w/10% calf serum |
Folkman, J., Haudenschild, C. C., and Zetter, B. R.: Long-term Culture of Capillary Endothlial Cells, Proc Natl Acad Sci U S A 76,
5217, 1979 |
Mouse
|
Mouse |
Chromaffin |
Collagenase Type 1: 0.1-0.2%
Deoxyribonuclease I: 0.003-0.015%
Hyaluronidase: 0.015%
|
DMEM/F12 |
Dominguez, N., Rodriguez, M., Machado, J. and Borges, R.: Preparation and Culture of Adrenal Chromaffin Cells., Methods Mol Biol 846,
223, 2012 |
Mouse
|
Mouse, 8-10 week |
Chromaffin |
Papain: 40 u/ml
|
DMEM |
Kolski-Andreaco, A., Cai, H., Currle, D., Chandy, K. and Chow, R.: Mouse Adrenal Chromaffin Cell Isolation., J Vis Exp
2,
129, 2007 |
Mouse
|
Mouse, embryonic |
Chromafin cells |
Papain: 20-25 u/ml
|
DMEM |
Tian Jin-Hua, Wu Zheng-Xing, Unzicker Michael, Lu Li, Cai Qian, Li Cuiling, Schirra Claudia, Matti Ulf, Stevens David, Deng Chuxia, Rettig Jens, Sheng Zu-Hang: The role of Snapin in neurosecretion: snapin knock-out mice exhibit impaired calcium-dependent exocytosis of large dense-core vesicles in chromaffin cells, J Neurosci 25,
10546-55, 2005 |
Ovine
|
Ovine, adult and fetal |
Adrenocortical |
Collagenase Type 1: 0.4%
|
DMEM/Ham's F12 |
Valego, N. and Rose, J.: A Specific CRH Antagonist Attenuates ACTH-Stimulated Cortisol Secretion in Ovine Adrenocortical Cells., Reprod Sci
17,
477, 2010 |
Ovine
|
Ovine, fetal |
Adrenocortical |
Collagenase Type 1: 0.4%
|
DMEM/Ham's F12 |
Valego, N., Su, Y., Carey, L., Young, S., Tatter, S., Wang, J. and Rose, J.: Hypothalamic-Pituitary Disconnection in Fetal Sheep Blocks the Peripartum Increases in Adrenal Responsiveness and Adrenal ACTH Receptor Expression., Am J Physiol Regul Integr Comp Physiol
289,
R410, 2005 |
Ovine
|
Ovine, adult |
Chromaffin cells |
Collagenase Type 2: 0.2%
Deoxyribonuclease I: 100 u/ml
|
Locke's solution |
Keating, D., Rychkov, G., Adams, M., Holgert, H., McMillen, I.C. and Roberts, M.: Opioid Receptor Stimulation Suppresses the Adrenal Medulla Hypoxic Response in Sheep by Actions on Ca(2+) and K(+) Channels., J Physiol
555,
489, 2004 |
Ovine
|
Ovine, 3 year |
Anterior pituitary |
Trypsin: 2.5%
Deoxyribonuclease I: 0.004%
|
DMEM |
Canny B J, O'Farrell K A, Clarke I J, Tilbrook A J: The influence of sex and gonadectomy on the hypothalamo-pituitary-adrenal axis of the sheep, J Endocrinol 162,
215-25, 1999 |
Rat
|
Rat |
Chromaffin |
Collagenase Type 1: 0.1-0.2%
Deoxyribonuclease I: 0.003-0.015%
Hyaluronidase: 0.015%
|
DMEM/F12 |
Dominguez, N., Rodriguez, M., Machado, J. and Borges, R.: Preparation and Culture of Adrenal Chromaffin Cells., Methods Mol Biol 846,
223, 2012 |
Rat
|
Rat |
Chromaffin |
Collagenase Type 1: 0.26%
Deoxyribonuclease I: 0.015%
Hyaluronidase: 0.015%
|
HBSS |
Gilabert, J, Montalvo, G, and Artalejo A.: Rat Chromaffin Cells Primary Cultures: Standardization and Quality Assessment for Single-Cell Assays, Nat Protoc
,
294, 2006 |
Rat
|
Rat, SD |
Chromaffin cells |
Collagenase Type 1: 0.26%
Deoxyribonuclease I: 0.015%
Hyaluronidase: 0.015%
|
HBSS |
Gilabert, J: Necessary Conditions to Maintain Rat Adrenal Chromaffin Cells in Primary Culture,Cell Biology of the Chromaffin Cell,Borges, R. and Gandia, L.,
Instituto Teofilo Hernando,
, 2004 |
Rat
|
Rat, SD, male |
Zona fasciculata/reticularis |
Collagenase: 0.4%
|
Krebs-HEPES |
Bruder Eric D, Ball Dennis L, Goodfriend Theodore L, Raff Hershel: An oxidized metabolite of linoleic acid stimulates corticosterone production by rat adrenal cells, Am J Physiol Regul Integr Comp Physiol
284,
R1631-5, 2003 |
Rat
|
Rat, Wistar, newborn |
Chromaffin cells |
Collagenase Type 1: 0.025%
Deoxyribonuclease I: 0.0015%
|
DMEM |
Zhang L, Castell A, Avila E, Drucker-ColĂn R, Escobar A: Immunocytochemical, ultrastructural and neurochemical evidences on synaptogenesis and dopamine release of rat chromaffin cells co-cultured with striatal neurons, J Neuropathol Exp Neurol 59,
170-4, 2000 |
Rat
|
Rat, SD, male |
ZG ZFR |
Collagenase Type 1: 0.2%
|
Kreb's |
Sayed, S., Whitehouse, B., and Jones, P.: Phosphoserine/Threonine Phosphatases in the Rat Adrenal Cortex: A Role in the Control of Steroidogenesis, J Endocrinol 154,
449, 1997 |
Rat
|
Rat, Fischer, male, 10-16 weeks |
Adrenocortical |
Deoxyribonuclease I: 0.005%
|
BSS |
Roskelley, C.D. and Auersperg, N.: Density Separation of Rat Adrenocortical Cells: Morphology, Steroidogenesis, and P-450scc Expression in Primary Culture, In Vitro Cell Dev Biol 26,
493, 1990 |
Rat
|
Rat, male, 120-160 g, Rat, SD, male, 400-450 g |
Leydig Adrenal |
Collagenase Type 2: 0.03% (adrenal)
|
Krebs Ringer bicarbonate buffer |
Ng, T. and Liu, W.: Toxic Effect of Heavy Metals on Cells Isolated from the Rat Adrenal and Testis, In Vitro Cell Dev Biol 26,
24, 1990 |
Rat
|
Rat, SD, 2-4 day old (also bovine) |
Heart Adrenal chromaffin Paraneurons |
Trypsin: 0.06%
|
25mM HEPES buffered Locke's solution, CMF |
Trifaro, J., Tang, R., and Novas, M.: Monolayer Co-Culture of Rat Heart Cells and Bovine Adrenal Chromaffin Paraneurons, In Vitro Cell Dev Biol 26,
335, 1990 |
Rat
|
Rat, Long-Evans, female, 150-200 g |
Glomerulosa |
Collagenase: 0.2%
|
MEM-d-Val |
Payet, N., Deziel, Y., and Lehoux, J.-G.: Vasopressin: A Potent Growth Factor in Adrenal Glomerulosa Cells in Culture, J Steroid Biochem 20,
449, 1984 |
Rat
|
Rat, Fischer, male, 1-10 months |
Adrenocortical |
Deoxyribonuclease I: 0.005%
|
BSS |
Leonard, R.K., Auersperg, N., and Parkes, C.O.: Ascorbic Acid Accumulation by Cultured Rat Adrenocortical Cells, In Vitro 19,
46, 1983 |
Rat
|
Rat, SD, male, 400-450 g |
Decapular Capsular Glomerulosa |
Deoxyribonuclease I: 0.01%
|
Medium 199 |
Li, C.H., Ng, T.B., and Cheng, C.H.K.: Melanotropins: Aldosterone- and Corticosterone-Stimulating Activity in Isolated Rat Adrenal Cells, Int J Pept Protein Res 19,
361, 1982 |
Rat
|
Rat |
Chromaffin |
Trypsin: 0.10%
|
Ham's F-12 w/HEPES |
Englert, D.F.: An Optical Study of Isolated Rat Adrenal Chromaffin Cells, Exp Cell Res
125,
369, 1980 |
Rat
|
Rat, Hanover-Wistar, 2nd postnatal week (also guinea-pig, cattle) |
Chromaffin |
Collagenase: 0.5%
Collagenase: 0.5%
|
HBSS |
Unsicker, K., Rieffert, B., and Ziegler, W.: Effects of Cell Culture Conditions, Nerve Growth Factor, Dexamethasone, and Cyclic AMP on Adrenal Chromaffin Cells In Vitro, Adv Biochem Psychopharmacol 255,
51, 1980 |
Rat
|
Rat , SD, female, 200 g |
Glomerulosa |
Deoxyribonuclease I: 0.05%
|
Kreb's |
Braley, L., Williams, G., and Bradwin, G.: The Effect of Unit Gravity Sedimentation on Adrenal Steroidogenesis by Isolated Rat Glomerulosa and Fasciculata Cells, Endocrinology 106 (1),
50, 1980 |
Rat
|
Rat |
Foreskin |
Collagenase: 0.5%
|
Dulbecco's MEM w/10% calf serum |
Folkman, J., Haudenschild, C. C., and Zetter, B. R.: Long-term Culture of Capillary Endothlial Cells, Proc Natl Acad Sci U S A 76,
5217, 1979 |
Rat
|
Rat, Wistar-Hanover, 7-12 day |
Medullary |
Trypsin: 0.125%
|
HBSS |
Unsicker, K., Krisch, B., Otten, U., and Thoenen, H.: Nerve Growth Factor-Induced Fiber Outgrowth From Isolated Rat Adrenal Chromaffin Cells: Impairment by Glucocorticoids, Proc Natl Acad Sci U S A 75 (7),
3498, 1978 |
Rat
|
Rat, SD, male |
Cortical |
Trypsin: 0.25%
|
Kreb's Ringer bicarbonate buffer |
Barofsky, A., Feinstein, M., and Halkerston, I.: Enzymatic and Mechanical Requirements for the Dissociation of Cortical Cells From Rat Adrenal Glands, Exp Cell Res
79,
263, 1973 |
Rat
|
Rat, Holtzman, male, 180-250 g |
Adrenal |
Collagenase Type 1: 0.5%
|
Kreb's Ringer bicarbonate buffer |
Kloppenborg, P., Island, D., Liddle, G., Michelakis, A., and Nicholson, W.: A Method of Preparing Adrenal Cell Suspensions and Its Applicability to the In Vitro Study of Adrenal Metabolism, Endocrinology 82,
1053, 1968 |
|
|