Tissue Dissociation References | |
Tissue Type: Aorta |
Aorta: Bovine | |
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Warshaw, D., Szarek, J., Hubbard, M., and Evans, J.: Pharmacology and Force Development of Single Freshly Isolated Bovine Cartoid Artery Smooth Muscle Cells, Circ Res 58, 399, 1986 | |
Cells: | Vascular smooth muscle cells |
Enzyme(s) | Collagenase Type I: 500 U/ml Elastase Type III: 50 U/ml Deoxyribonuclease: 60 U/ml |
Medium | PSS |
Aorta: Human | |
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Eskin, S., Sybers, H., Lester, J., Navarro, L., Gotto, A., and DeBakey, M.: Human Smooth Muscle Cells Cultured From Atherosclerotic Plaques and Uninvolved Vessel Wall, In Vitro 17 (8), 713, 1981 | |
Cells: | Smooth muscle cells |
Enzyme(s) | Collagenase: 10 ml 0.1% Elastase: 0.05% Trypsin: 0.25% |
Medium | DMEM |
Aorta: Rat | |
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Cornwell, T., and Lincoln, T.: Regulation of Intracellular Ca2+ Levels in Cultured Vascular Smooth Muscle Cells, J Biol Chem 264 (2), 1146, 1989 | |
Cells: | Aortic smooth muscle cells |
Enzyme(s) | Collagenase Type III: 200 units/ml Elastase: 0.1 mg Soybean Trypsin Inhibitor: 0.5 mg/ml |
Medium | DMEM |
Aorta: Rat | |
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Cole, O., Fan, T., and Lewis, G.: Isolation, Characterization, Growth and Culture of Endothelial Cells From the Rat Aorta, Cell Biol Int Rep 10 (6), 399, 1986 | |
Cells: | Endothelial cells |
Enzyme(s) | Collagenase Dispase II Trypsin Elastase Hyaluronidase |
Medium | RPMI 1640 |
Aorta: Rat | |
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Gordon, D., Mohai, L., and Schwartz, S.: Induction of Polyploidy in Cultures of Neonatal Rat Aortic Smooth Muscle Cells, Circ Res 59, 633, 1986 | |
Cells: | Endothelial cells |
Enzyme(s) | Collagenase Type I: 0.1% Elastase: 0.05% |
Medium | Waymouth's culture medium |
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