Tissue Dissociation References


	Tissue Dissociation References
	Tissue Type: Aortae

Aortae: Bovine
Bovine(also rat)
	Steinberg, S., Bilezikian, J., and Al-Awqati, Q.: Fura-2 Flourescence is Localized to Mitochondria in Endothelial Cells, Am J Physiol 253, C744, 1987
Cells:	Endothelial cells
Enzyme(s)
Medium	HEPES
Aortae: Bovine
Bovine
	Davies, P., Remuzzi, A., Gordon, E., Dewey, C., and Gimbrone, M.: Turbulent Fluid Shear Stress Induces Vascular Endothelial Cell Turnover In Vitro, Proc Natl Acad Sci U S A 83, 2114, 1986
Cells:	Endothelial cells
Enzyme(s)
Medium	(see reference)
Aortae: Calf
Calf
	Howard, B., Macarak, E., Gunson, D., and Kefalides, N.: Characterization of the Collagen Synthesized by Endothelial Cells in Culture, Proc Natl Acad Sci U S A 73 (7), 2361, 1976
Cells:	Endothelial cells
Enzyme(s)	Collagenase Type I: 10-15 ml
Medium	DMEM
Aortae: Pig
Pigs
	Vischer, P., and Buddecke, E.: Alteration of Glycosytransferase Activities during Proliferation of Cultivated Arterial Endothelial Cells and Smooth Muscle Cells, Exp Cell Res 156, 15, 1985
Cells:	Endothelial cells
Enzyme(s)	Collagenase Trypsin: 0.05%
Medium	DMEM
Aortae: Rat
10 wk old female Wistar rats
	Boulanger-Saunier, C., Kattenburg, D., and Stoclet, J.: Cyclic AMP-Dependent Phosphorylation of a 16 kDa Protein in a Plasma Membrane-Enriched Fraction of Rat Aortic Myocytes, FEBS Lett 193 (2), 283, 1985
Cells:	Myocytes
Enzyme(s)	Collagenase
Medium	(see reference)