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Tissue Dissociation References
Tissue Type: Blood


Blood: Human
  • Fifteen patients, ages 6-58 yrs.
Pastorelli, G., Roncarolo, M., Touraine, J., Peronne, G., Tovo, P., and Vries, J.: Peripheral Blood Lymphocytes of Patients With Common Variable Immunodeficiency (CVI) Produce Reduced Levels of Interleukin-4, Interleukin-2 and Interferon-Gamma, but Proliferate Normally Upon Activation by Mitogens, Clin Exp Immunol 78, 334, 1989
Cells:     Peripheral Blood Lymphocytes
Enzyme(s)    Alkaline Phosphatase
Medium    RPMI 1640


Blood: Human
  • HIV positive humans
Production of Tumour Necrosis Factor-Alpha (TNF-a) and Interleukin-1 (IL-1) in Patients with AIDS. Enhanced Level of TNF-a is Related to a Higher Cytotoxic Activity: , Clin Exp Immunol 78, 329, 1989
Cells:     Mononuclear cells
Enzyme(s)    
Medium    RPMI 1640


Blood: Human
  • Human
Scanlon, M., Williams, D., and Fay, F.: A Ca2+-Insensitive Form of Fura -2 Associated with Polymorphonuclear Leukocytes, J Biol Chem 262 (13), 6308, 1987
Cells:     Polymorphnuclear leukocytes
Enzyme(s)    
Medium    HBSS


Blood: Human
  • Human
Traganos, F., Gorski, A., Darzynkiewicz, Z., Sharpless, T., and Melamed, M.: Rapid Multiparameter Analysis of Cell Stimulation in Mixed Lymphocyte Culture Reactions, J Histochem Cytochem 25 (7), 881, 1977
Cells:     Lymphocytes
Enzyme(s)    Ribonuclease A: 1000 units/ml
Deoxyribonuclease: 1000 units/ml
Medium    HBSS


Blood: Human
  • Human
Darzynkiewicz, Z., Traganos, F., Sharpless, T., and Melamed, M.: Lymphocyte Stimulation: A Rapid Multiparameter Analysis, Proc Natl Acad Sci U S A 73 (8), 2881, 1976
Cells:     Mononuclear cells
Enzyme(s)    
Medium    (see reference)

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