Bone - Tissue Tables
Species | Species Detail | Cells | Enzyme(s) | Medium |
Reference |
Bovine
|
Bovine |
Chondrocytes |
Collagenase Type 2: 0.4%
|
DMEM |
Buschmann, M., Gluzband, Y., Grodzinsky, A., and Hunziker, E.: Mechanical Compression Modulates Matrix Biosynthesis in Chondrocyte/Agarose Culture, J Cell Sci
108,
1497, 1995 |
Chicken
|
Chick, day old |
Osteoblasts |
Trypsin: 0.03%
|
DMEM |
Gay, C., Lloyd, Q., and Gilman, V.: Characteristics and Culture of Osteoblasts Derived From Avian Long Bone, In Vitro Cell Dev Biol 30A,
379, 1994 |
Chicken
|
Chick, Peterson/Arbor Acre, male, 4 weeks old (Gallus domesticus) |
Chondrocytes |
Trypsin: 0.25%
|
Ham's F12 |
Rosselot, G., Reginato, A.M., and Leach, R.M.: Development of a Serum-Free System to Study the Effect of Growth Hormone and Insulinlike Growth Factor-1 on Cultured Postembryonic Growth Plate Chondrocytes, In Vitro Cell Dev Biol 28A,
235, 1992 |
Chicken
|
Chick, embryo |
Vertebrae chondroblasts |
Trypsin: 0.25%
|
Simm's, CMF |
Schiltz, J.R., Mayne R., and Holtzer, H.: The Synthesis of Collagen and Glycosaminoglycans by Dedifferentiated Chondroblasts in Culture, Differentiation
1,
97, 1973 |
Human
|
Human |
Bone-cartilage-stromal |
Collagenase Type 2: 0.75%
|
HBSS |
Agarwal, S., Loder, S., Sorkin, M., Li, S., Shrestha, S., Zhao, B., Mishina, Y., James, A. and Levi, B.: Analysis of Bone-Cartilage-Stromal Progenitor Populations in Trauma Induced and Genetic Models of Heterotopic Ossification., Stem Cells 34,
1692-701, 2016 |
Human
|
Human |
Osteoblasts |
Collagenase Type 2: 0.2%
Trypsin: 0.25%-1.0%
|
DMEM |
Taylor, S., Shah, M. and Orriss, I.: Generation of Rodent and Human Osteoblasts., Bonekey Rep
3,
585, 2014 |
Human
|
Human |
Osteoblasts |
Collagenase Type 3: 0.2%
|
MEM |
Kode, A.,Manavalan1, J.,Mosialou1, I., Bhagat G., Rathinam, C, Luo, N et al.: Leukemogenesis Induced by an Activating β-catenin Mutation in Osteoblasts, Nature
506,
240, 2014 |
Human
|
Human, 22-73 year |
Osteoblasts |
Trypsin: 0.5%
|
Basal Medium |
Kneser U, Voogd A, Ohnolz J, Buettner O, Stangenberg L, Zhang YH, Stark GB, Schaefer DJ: Fibrin gel-immobilized primary osteoblasts in calcium phosphate bone cement: in vivo evaluation with regard to application as injectable biological bone substitute, Cells Tissues Organs
179,
158-69, 2005 |
Human
|
Human, 60+ year |
Bone Cells, Osteoblasts |
Collagenase Type 2: 200-250 u/ml
|
DMEM |
Chen X., Qian H., Neff L., Satomura K., and Horowitz M.: Thy-1 antigen Expression by Cells in the Osteoblast Lineage, J Bone Miner Res 14,
362, 1999 |
Human
|
Human |
Osteoblasts |
Trypsin: 0.1%
|
DMEM |
Meikle, M., Boyd, S., Hembry, R., Compston, J., Croucher, P., and Reynolds, J.: Human Osteoblasts in Culture Synthesize Collagenase and Other Matrix Metalloproteinases in Response to Osteotropic Hormones and Cytokines, J Cell Sci
103,
1093, 1992 |
Human
|
Human |
Osteoblasts |
Collagenase Type 4: 250 u/ml
|
DMEM |
Fedarko, N.S., Termine, J.D., Young, M.F. and Robey, P.G.: Temporal Regulation of Hyaluronan and Proteoglycan Metabolism by Human Bone Cells in Vitro, J Biol Chem 265,
12200, 1990 |
Mouse
|
Mouse |
Bone and bone marrow stromal |
Stemxyme 1: 0.1%
Neutral Protease: 0.1%
|
PBS |
Mohrin, D., Koukos, G., Alabdulaaly, L., Paw, J., Hake, K., Kolumam, G., Bouxsein, M., Baron, R., Kutskova, Y., Freund, A., Liu, J., Zavala-Solorio, J., Bhargava, S., Maxwell, Trumble, J., Brito, A., Hu, D. and Brooks, D.: Inhibition of Longevity Regulator PAPP-A Modulates Tissue Homeostasis via Restraint of Mesenchymal Stromal Cells.,Aging Cell Vol. 20,,
,
e13313, 2021 |
Mouse
|
Mouse |
Bone and bone marrow stroma |
Stemxyme 1: 0.1%
Neutral Protease: 0.1%
|
Media 199 |
Baryawno, N., Przybylski, D., Kowalczyk, M., Kfoury, Y., Severe, N., Gustafsson, K., Kokkaliaris, K., Mercier, F., Tabaka, M., Hofree, M., Dionne, D., Papazian, A., Lee, D., Ashenberg, O. and Subramanian, A.: A Cellular Taxonomy of the Bone Marrow Stroma in Homeostasis and Leukemia., Cell
177,
1915-1932.e16, 2019 |
Mouse
|
Mouse |
Osteoblast |
Collagenase Type 1: 0.3%
|
HBSS |
Wang, W., Majihail, G., Lui, C. and Zhou, L.: Osteoblast Sorting and Intracellular Staining of CXCL12., Bio Protoc
8,
, 2018 |
Mouse
|
Mouse |
Bone-cartilage-stromal |
Collagenase Type 2: 0.75%
|
HBSS |
Agarwal, S., Loder, S., Sorkin, M., Li, S., Shrestha, S., Zhao, B., Mishina, Y., James, A. and Levi, B.: Analysis of Bone-Cartilage-Stromal Progenitor Populations in Trauma Induced and Genetic Models of Heterotopic Ossification., Stem Cells 34,
1692-701, 2016 |
Mouse
|
Mouse |
Bone stromal |
Collagenase Type 1: 0.3%
|
DMEM |
Igarashi, H., Akahoshi, N., Ohto-Nakanishi, T., Yasuda, D. and Ishii, S.: The Lysophosphatidic Acid Receptor LPA4 Regulates Hematopoiesis-Supporting Activity of Bone Marrow Stromal Cells., Sci Rep
5,
11410, 2015 |
Mouse
|
Mouse |
Osteoblasts |
Collagenase Type 1: 1.0%
Neutral Protease: 1.6%
|
MEM |
Lee, D., Choung, H., Kim, H., Gronostajski, R., Yang, Y., Ryoo, H., Lee, Z., Kim, H., Cho, E. and Park, J.: NFI-C Regulates Osteoblast Differentiation Via Control of Osterix Expression., Stem Cells 32,
2467-79, 2014 |
Mouse
|
Mouse, 2-5 day |
Osteoblasts |
Collagenase Type 2: 0.2%
Trypsin: 0.25%-1.0%
|
DMEM |
Taylor, S., Shah, M. and Orriss, I.: Generation of Rodent and Human Osteoblasts., Bonekey Rep
3,
585, 2014 |
Mouse
|
Mouse |
Osteoblasts |
Collagenase Type 3: 0.2%
|
MEM |
Kode, A.,Manavalan1, J.,Mosialou1, I., Bhagat G., Rathinam, C, Luo, N et al.: Leukemogenesis Induced by an Activating β-catenin Mutation in Osteoblasts, Nature
506,
240, 2014 |
Mouse
|
Mouse, 4-22 month |
Osteocytes |
Collagenase Type 1: 300 u/ml
|
MEM |
Stern, A., Stern, M., Van Dyke, M., Jahn, K., Prideaux, M. and Bonewald, L.: Isolation and Culture of Primary Osteocytes from the Long Bones of Skeletally Mature and Aged Mice., Biotechniques
52,
361, 2012 |
Mouse
|
Mouse |
Endosteal cells |
Collagenase Type 1: 0.3%
|
DMEM |
Nakamura, Y., Arai, F., Iwasaki, H., Hosokawa, K., Kobayashi, I., Gomei, Y., Matsumoto, Y., Yoshihara, H. and Suda, T.: Isolation and Characterization of Endosteal Niche Cell Populations that Regulate Hematopoietic Stem Cells., Blood 116,
1422, 2010 |
Mouse
|
Mouse |
Bone marrow |
Collagenase: 0.2%
|
DMEM |
Morikawa, S., Mabuchi, Y., Kubota, Y., Nagai, Y., Niibe, K., Hiratsu, E., Suzuki, S., Miyauchi-Hara, C., Nagoshi, N., Sunabori, T., Shimmura, S., Miyawaki, A., Nakagawa, T., Suda, T., Okano, H. and Matsuzaki, Y.: Prospective Identification, Isolation, and Systemic Transplantation of Multipotent Mesenchymal Stem Cells in Murine Bone Marrow., J Exp Med 206,
2483-96, 2009 |
Mouse
|
Mouse, male, 6-8 week old |
Osteoclasts |
Collagenase Type 3: 0.1%
|
DMEM |
Sakai, E., Miyamoto, H., Okamoto, K., Kato, Y., Yamamoto, K., and Sakai, H.: Characterization of Phagosomal Subpopulations Along Endocytic Routes in Osteoclasts and Macrophages, J. Biochem.
130,
823, 2001 |
Mouse
|
Mouse, 3-5 day, 6-8 week |
Bone Cells, Osteoblasts |
Collagenase Type 2: 200-250 u/ml
|
DMEM |
Chen X., Qian H., Neff L., Satomura K., and Horowitz M.: Thy-1 antigen Expression by Cells in the Osteoblast Lineage, J Bone Miner Res 14,
362, 1999 |
Mouse
|
Mouse, BALB/c |
Osteoblast-like Cells Stromal Cell Lines Hematopoietic Blast Cells |
Trypsin: 0.1%
|
Eagle's MEM |
Takanashi, H., Matsuishi, T., and Yoshizato, K.: Establishment and Characterization of Stromal Cell Lines That Support Differentiation of Murine Hematopoietic Blast Cells into Osteoblast-like Cells, In Vitro Cell Dev Biol 30A,
384, 1994 |
Mouse
|
Mouse, Swiss-Webster |
Neonatal bone |
Collagenase Type 2: 0.20%
|
Tris-buffered saline |
Chen,T. and Feldman,D.: Regulation of 1,25-Dihydroxyvitamin D3 Receptors in Cultured Mouse Bone Cells, J Biol Chem 256,
5561, 1981 |
Rat
|
Rat, 2 day |
Osteoblasts |
Collagenase Type 2: 0.1%
Trypsin: 0.25%
|
DMEM |
Jeon, J., Lee, M. and Yang, H.: Differentiated Osteoblasts Derived Decellularized Extracellular Matrix to Promote Osteogenic Differentiation., Biomater Res
22,
4, 2018 |
Rat
|
Rat, Wistar, 250g |
Proximal femur stem |
Collagenase Type 1: 0.075%
|
HBSS |
Jacobs, F., Gijsen, H., Van de Vyver, M. and Ferris, W.: Vanadate Impedes Adipogenesis in Mesenchymal Stem Cells Derived from Different Depots within Bone., Front Endocrinol
7,
108, 2016 |
Rat
|
Rat, 2-3 day |
Osteoblasts |
Collagenase Type 2: 0.2%
Trypsin: 0.25%-1.0%
|
DMEM |
Taylor, S., Shah, M. and Orriss, I.: Generation of Rodent and Human Osteoblasts., Bonekey Rep
3,
585, 2014 |
Rat
|
Rat, fetal, 21 days of gestation |
Calvaria |
Collagenase: 0.2%
|
MEM |
Owen, T., Aronow, M., Shalhoub, V., Barone, L., Wilming, L., Tassinari, M., Kennedy, M., Pockwinse, S., Lian, J., and Stein, G.: Progressive Development of the Rat Oseoblast Phenotype In Vitro: Reciprocal Relationships in Expression of Genes Associated with Osteoblast Proliferation and Differentiation During Formation of the Bone Extracellular Matrix, J Cell Physiol 143,
420, 1990 |
Rat
|
Rat, newborn |
Osteoblastlike cells |
Collagenase Type 2: 0.3%
|
MEM |
Ernst, M., and Froesch, E.: Osteoblastlike Cells in a Serum-Free Methylcellulose Medium Form Colonies: Effects of Insulin and Insulinlike Growth Factor I, Calcif Tissue Int 40,
27, 1987 |
Rat
|
Rat, fetus, 17-21 day |
Calvaria |
Collagenase: 0.01%-0.6%
|
Tris-buffered saline |
Peck, W., Birge, S., and Fedak, S.: Bone Cells: Biochemical and Biological Studies After Enzymatic Isolation, Science 146,
1476, 1964 |
|
|