Brain - Tissue Tables
Species | Species Detail | Cells | Enzyme(s) | Medium |
Reference |
Bovine
|
Bovine |
Microvascular endothelial |
Neutral Protease: 0.005%
|
Medium 199 |
Kanda, T., Yoshino, H., Ariga, T., Yamawaki, M., and Yu, R.: Glycosphingolipid Antigens in Cultured Microvasular Bovine Brain Endothelial Cells: Sulfoglucuronosyl Paragloboside as a Target of Monoclonal IgM in Demyelinative Neuropathy, J Cell Biol
126 (1),
235, 1994 |
Bovine
|
Bovine |
Brain endothelial cells |
Collagenase Type 2: 0.35%
|
DMEM |
Wolburg H, Neuhaus J, Kniesel U, Krauss B, Schmid EM, Ocalan M, Farrell C, Risau W: Modulation of tight junction structure in blood-brain barrier endothelial cells. Effects of tissue culture, second messengers and cocultured astrocytes, J Cell Sci
107,
1347, 1994 |
Bovine
|
Bovine |
Endothelial |
Neutral Protease: 0.125%
|
MEM |
Miller, D., Audus, K., and Borchardt, R.: Application of Cultured Endothelial Cells of the Brain Microvasculature in the Study of the Blood-Brain Barrier, J Tiss Cul Meth 14,
217, 1992 |
Bovine
|
Bovine, adult |
Cerebral artery Endothelial |
Collagenase: 0.2%
|
HBSS |
Machi, T., Kassell, N.F., and Scheld, W.M.: Isolation and Characterization of Endothelial Cells From Bovine Cerebral Arteries, In Vitro Cell Dev Biol 26,
291, 1990 |
Bovine
|
Bovine |
Capillary endothelial |
Collagenase: 0.1%
|
DMEM |
Estrada, C., Bready, J., Berliner, J., and Cancilla, P.: Choline Uptake by Cerebral Capillary Endothelial Cells in Culture, J Neurochem 54,
1467, 1990 |
Bovine
|
Bovine |
Endothelial |
Neutral Protease: 0.5%
|
MEM |
Audus, K., and Borchardt, R.: Characterization of an In Vitro Blood-Brain Barrier Model System for Studying Drug Transport and Metabolism, Pharm Res 3 (2),
81, 1986 |
Bovine
|
Bovine |
Endothelial Brain arteries |
Collagenase Type 2: 0.2%
|
Dulbecco's PBS |
Goetz, I.., Warren, J., Estrada, C., Roberts, E., and Krause, D.: Long-Term Cultivation of Arterial and Capillary Endothelium From Adult Bovine Brain, In Vitro Cell Dev Biol 21,
172, 1985 |
Bovine
|
Calf (also lamb) |
Oligodendroglia Neural |
Trypsin: 0.1%
|
(see reference) |
Poduslo, S., Miller, K., and McKhann, G.: Metabolic Properties of Maintained Oligodendroglia Purified from Brain, J Biol Chem 253,
1592, 1978 |
Guinea-Pig
|
Guinea-pig, 200-400 g |
Neurons |
Trypsin: 0.06-0.08%
|
PIPES saline |
Kay, A.R., and Wong, R.K.S.: Isolation of Neurons Suitable for Patch-Clamping from Adult Mammalian Central Nervous Systems, J Neurosci Methods 16,
227, 1986 |
Human
|
Human |
Microglia |
Collagenase Type 1: 300 u/ml
Trypsin: 0.125%
|
DMEM |
Mizee, M., Miedema, S., van der Poel, M., Adelia , S., van Strien, M., Melief, J., Smolders, J., Hendrickx, D., Heutinck, K., Hamann, J. and Huitinga, I.: Isolation of Primary Microglia from the Human Post-Mortem Brain: Effects of Ante- and Post-Mortem Variables., Acta Neuropathol
5,
16, 2017 |
Human
|
Human, adult |
Neuronal |
Papain: 20 u/ml
|
Neurobasal |
Spaethling, J., Na, Y., Lee, J., Ulyanova, A., Baltuch, G., Bell, T., Brem, S., Chen, H., Dueck, H., Fisher, S., Garcia, M., Khaladkar, M., Kung, D., Lucas, T., O'Rourke, D. and Stefanik, D.: Primary Cell Culture of Live Neurosurgically Resected Aged Adult Human Brain Cells and Single Cell Transcriptomics., Cell Rep
18,
791-803, 2017 |
Human
|
Human, fetal and mature |
Astrocytes and neurons |
Papain: 7.5-20 u/ml
|
RPMI |
Zhang, Y., Sloan, S., Clarke, L., Caneda, C., Plaza, C., Blumenthal, P., Vogel, H., Steinberg, G., Edwards, M., Li, G., Duncan, J., Cheshier, S., Shuer, L., Chang, E., Grant, G., Gephart, M. and Barres, B.: Purification and Characterization of Progenitor and Mature Human Astrocytes Reveals Transcriptional and Functional Differences with Mouse., Neuron 89,
37-53, 2016 |
Human
|
Human |
Microglia |
Neutral Protease: 10 u/ml
Papain: 2.5 u/ml
|
Hibernate A |
Rustenhoven, J., Park Thomas, I., Schweder, P., Scotter, J., Correia, J., Smith, A., Gibbons, H., Oldfield, R., Bergin, P., Mee, E., Faull, R., Curtis, M., Scott, G. and Dragunow, M.: Isolation of Highly Enriched Primary Human Microglia for Functional Studies., Sci Rep
6,
19371, 2016 |
Human
|
Human, 25-81 yr |
Brain tumor |
Neutral Protease: 0.11 u/ml
Collagenase Type 4: 0.05%
Hyaluronidase: 1,000 u/ml
Deoxyribonuclease I: 5 u/ml
|
HBSS |
Volovitz, I, Shapira, N., Ezer, H., Gafni, A., Lustgarten, M., Alter, T., Ben-Horin, I., Barzilai, O., Shahar, T., Kanner, A., Fried, I., Veshchev, I., Grossman, R. and Ram, Z,: A Non-Aggressive, Highly Efficient, Enzymatic Method for Dissociation of Human Brain-Tumors and Brain-Tissues to Viable Single-Cells., BMC Neurosci
17,
30, 2016 |
Human
|
Human |
Amyloid Fibrils |
Collagenase Type 3: 0.02%
Deoxyribonuclease I: 0.001%
|
50mM Tris |
Scherpelz, K., Lu, J.,Tycko, R. and Meredith, S.: Preparation of Amyloid Fibrils Seeded from Brain and Meninges, Methods Mol Biol 1345,
299, 2016 |
Human
|
|
Brain tumor |
Collagenase Type 1: 0.04%
Hyaluronidase: 0.01%
Deoxyribonuclease I: 0.02%
Neutral Protease: 0.008%
|
DMEM/F12 |
Hussein, D., Punjaruk, W., Storer, L., Shaw, L., Othman, R., Ottoman, R., Peet, A., Miller, S., Bandopadhyay, G., Heath, R., Kumari, R., Bowman, K., Braker, P., Rahman, R., Jones, G., Watson, S. and Lowe, J.: Pediatric Brain Tumor Cancer Stem Cells: Cell Cycle Dynamics, DNA Repair, and Etoposide Extrusion., Neuro Oncol
13,
70-83, 2011 |
Human
|
Human |
Tumor |
Collagenase Type 4: 0.1%
Hyaluronidase: 0.07%
Deoxyribonuclease I: 0.04%
|
see reference |
Sauvageot, C., Weatherbee, J., Kesari, S., Winters, S., Barnes, J., Dellagatta, J., Ramakrishna, N., Stiles, C., Kung, A., Kieran, M. and Wen, P.: Efficacy of the HSP90 Inhibitor 17-AAG in Human Glioma Cell Lines and Tumorigenic Glioma Stem Cells.,Neuro Oncol Vol. 11,,
,
109, 2009 |
Human
|
Human |
Microglia |
Trypsin: 0.25%
Deoxyribonuclease I: .005%
|
DMEM/F12 |
Klegeris Andis, McGeer PatrickL: Chymotrypsin-like proteases contribute to human monocytic THP-1 cell as well as human microglial neurotoxicity, Glia 51,
56-64, 2005 |
Human
|
Human |
Microvessels |
Collagenase Type 4: 0.1%
|
DMEM |
Gerhart, D. Z., Broderius, M. A., and Drewes, L. R.: Cutlured Human and Canine Endothelial Cells from Brain Microvessels, Brain Res Bull 21,
785, 1988 |
Human
|
Human |
Neuronal |
Deoxyribonuclease I: 10 µg/ml
|
Tris-HCl, 50 mM, CaCl2, 2 mM |
Roher, A.E., Palmer, K.C., Chau, V., and Ball, M.J.: Isolation and Chemical Characterization of Alzheimer's Disease Paired Helical Filament Cytoskeletons:Differentiation from Amyloid Plaque Core Protein, J Cell Biol
107,
2703, 1988 |
Human
|
Human, 15-54 years |
Microvessels |
Collagenase: 0.1%
|
Serum-free modified Lewis medium |
Vinters, H.V., Reave, S., Costello, P., Girvin, J.P., and Moore, S.A.: Isolation and Culture of Cells Derived From Human Cerebral Microvessels, Cell Tissue Res 249,
657, 1987 |
Insect
|
Drosophilia |
Neurons |
Papain: 50 u/ml
|
Modified Dissecting Saline |
Hadzic, T., Park, D., Abruzzi, K., Yang, L., Trigg, J., Rohs, R., Rosbash, M. and Taghert, P.: Genome-Wide Features of Neuroendocrine Regulation in Drosophila by the Basic Helix-Loop-Helix Transcription Factor DIMMED., Nucleic Acids Res
43,
2199-215, 2015 |
Insect
|
Drosophila |
Neurons |
Papain: 20 u/ml
|
Saline |
Gu, H. and O'Dowd, D.: Whole Cell Recordings from Brain of Adult Drosophila., J Vis Exp
6,
248, 2007 |
Insect
|
Drosophila |
Neurons |
Papain: 4 u/ml
|
DMEM |
Sicaeros, B. Campusano, J. and O'Dowd, D.: Primary Neuronal Cultures from the Brains of Late Stage Drosophila Pupae., J Vis Exp
4,
200, 2007 |
Insect
|
Drosophilia |
Kenyon |
Papain: 20 u/ml
|
Recording saline |
Gu, H. and O'Dowd, D.: Cholinergic Synaptic Transmission in Adult Drosophila Kenyon Cells in Situ., J Neurosci 26,
265-72, 2006 |
Monkey
|
Monkey, rhesus |
Brain cells |
Collagenase Type 2: 500 u/ml
Deoxyribonuclease I: 28 u/ml
|
HBSS |
Marcondes MC, Burudi EM, Huitron-Resendiz S, Sanchez-Alavez M, Watry D, Zandonatti M, Henriksen SJ, Fox HS: Highly activated CD8(+) T cells in the brain correlate with early central nervous system dysfunction in simian immunodeficiency virus infection, J Immunol 167,
5429-38, 2001 |
Mouse
|
Adult and neonatal |
Microglia, astrocytes, lymphocytes |
Collagenase Type 2: 120 u/ml
Papain: 20 u/ml
Neutral Protease: 1.2 u/ml
Deoxyribonuclease I: 20 u/ml
|
EBSS |
Calvo, Belen, Rubio, F., Fernandez, M. and Tranque, P.: Dissociation of Neonatal and Adult Mice Brain for Simultaneous Analysis of Microglia, Astrocytes and Infiltrating Lymphocytes by Flow Cytometry.,IBRO Rep Vol. 8,,
,
36-47, 2020 |
Mouse
|
Mouse, adult |
Astrocytes, microglia, endothelia, oligodendrocytes |
Papain: 20 u/ml
Collagenase Type 1: 0.01%
|
HBSS |
Swartzlander, D., Propson, N., Roy, E., Saito, T., Saido, T., Wang, B. and Zheng, H.: Concurrent Cell Type-Specific Isolation and Profiling of Mouse Brains in Inflammation and Alzheimer's Disease., JCI Insight
3,
, 2018 |
Mouse
|
Mouse |
Neurons |
Papain: 10 u/ml
Deoxyribonuclease I: 10 u/ml
|
Artificial CSF |
Kramer, D., Risso, D., Kosillo, ., Ngai, J. and Bateup, H.: Combinatorial Expression of Grp and Neurod6 Defines Dopamine Neuron Populations with Distinct Projection Patterns and Disease Vulnerability,eNeuro Vol. 5,,
,
1, 2018 |
Mouse
|
Mouse, fetal and mature |
Astrocytes and neurons |
Papain: 7.5-20 u/ml
|
RPMI |
Zhang, Y., Sloan, S., Clarke, L., Caneda, C., Plaza, C., Blumenthal, P., Vogel, H., Steinberg, G., Edwards, M., Li, G., Duncan, J., Cheshier, S., Shuer, L., Chang, E., Grant, G., Gephart, M. and Barres, B.: Purification and Characterization of Progenitor and Mature Human Astrocytes Reveals Transcriptional and Functional Differences with Mouse., Neuron 89,
37-53, 2016 |
Mouse
|
Mouse |
Chloroid plexus |
Collagenase Type 4: 400 u/ml
|
PBS |
Baruch, K., Rosenzweig, N., Kertser, A., Deczkowska, A., Sharif, A., Spinrad, A., Tsitsou-Kampeli, A., Sarel, A., Cahalon, L. and Schwartz, M.: Breaking Immune Tolerance by Targeting Foxp3(+) Regulatory T Cells Mitigates Alzheimer's Disease Pathology., Nat Commun
6,
7967, 2015 |
Mouse
|
Mouse, 3 week |
Neural |
Papain: 20 u/ml
|
PBS |
Luo,Y.,Coskun, V., Liang, A.,Yu, J., Cheng, L.,Ge, W., Shi, Z., Zhang, K., Li, C., Lin, H., Luo, D., Wang, J., Lin, C., Dai, Z., Zhu, H., Zhang, J., Liu, J., Liu, H.,deVellis, J., Horvath, S., Sun, Y. and Li, S.: Different Patterns of Molecular Forms of Somatostatin are Released by the Rat Median Eminence and Hypothalamus., Cell
161,
1175, 2015 |
Mouse
|
Mouse |
Brain-sequestered leukocytes |
Collagenase Type 4: 0.05%
Deoxyribonuclease I: 2 u/ml
|
RPMI |
Ryg-Cornejo, V., Ioannidis, L. and Hansen, D.: Isolation and Analysis of Brain-Sequestered Leukocytes from Plasmodium Berghei ANKA-Infected Mice., J Vis Exp
71,
e50112, 2013 |
Mouse
|
Mouse |
Cerebral pericytes |
Collagenase Type 2: 0.1%
Deoxyribonuclease I: 30 u/ml
|
DMEM |
Shah. G., Price, T., Banks, W., Morofuji, Y., Kovac, A., Ercal, N., Sorenson, C., Shin, E., Sheibani, N.: Pharmacological Inhibition of Mitochondrial Carbonic Anhydrases Protects Mouse Cerebral Pericytes from High Glucose-Induced Oxidative Stress and Apoptosis., J Pharmacol Exp Ther 344,
637-45, 2013 |
Mouse
|
Mouse, P13-15 |
Glial |
Papain: 20 u/ml
Deoxyribonuclease I: 0.0005%
|
EBSS |
Haseleu, J., Anlauf, E., Blaess, S., Endl, E. and Derouiche, A.: Studying Subcellular Detail in Fixed Astrocytes: Dissociation of Morphologically Intact Glial Cells (DIMIGs)., Front Cell Neurosci
7,
54, 2013 |
Mouse
|
Mouse, P0-P15 |
Astrocytes |
Papain: 5 u/ml
Deoxyribonuclease I: 0.004%
|
DPBS |
Foo, L.: Purification of Rat and Mouse Astrocytes by Immunopanning., Cold Spring Harb. Protoc. 2013,
421-32, 2013 |
Mouse
|
Mouse, adult |
Neurons |
PDS kit: with modifications
|
DMEM/F12 |
Saxena, A., Wagatsuma, A., Noro, Y., Kuji, T., Asaka-Oba, A., Watahiki, A., Gurnot, C., Fagiolini, M., Hensch, T. and Carninci, P.: Trehalose-Enhanced Isolation of Neuronal Sub-Types from Adult Mouse Brain., Biotechniques
52,
381, 2012 |
Mouse
|
Mouse, 1-2 day |
Oligodendrocytes, dorsal root ganglia |
Papain: 0.15%
Deoxyribonuclease I: 0.006%
|
DMEM |
O'Meara, R., Ryan, S., Colognato, H. and Kothary, R.: Derivation of Enriched Oligodendrocyte Cultures and Oligodendrocyte/Neuron Myelinating Co-Cultures from Post-Natal Murine Tissues., J Vis Exp
54,
3324, 2011 |
Mouse
|
Mouse, 6-12 week |
Vascular smooth muscle cells |
Papain: 0.05%
Collagenase Type 4: 0.15%
Elastase: 0.05%
|
PBS |
Chung, W., Farley, J., Swenson, A., Barnard, J., Hamilton, G., Chiposi, R. and Drummond, H.: Extracellular Acidosis Activates ASIC-like Channels in Freshly Isolated Cerebral Artery Smooth Muscle Cells., Am J Physiol Cell Physiol
298,
C1198, 2010 |
Mouse
|
Mouse, 4-6 day |
Neurons |
PDS kit: with modifications
|
HBSS |
Lee, H., Greene, L., Mason, C. and Manzini, M.: Isolation and Culture of Post-Natal Mouse Cerebellar Granule Neuron Progenitor Cells and Neurons., J Vis Exp
23,
990, 2009 |
Mouse
|
Mouse, postnatal |
Astrocytes |
Trypsin: 0.25%
Deoxyribonuclease I: 1,000 u/ml
|
HBSS |
Sher, F., Rossler, R., Brouwer, N., Balasubramaniyan, V., Boddeke, E. and Copray, S.: Differentiation of Neural Stem Cells Into Oligodendrocytes: Involvement of the Polycomb Group Protein Ezh2., Stem Cells 26,
2875, 2008 |
Mouse
|
Mouse, neonatal |
Neurons |
Papain: 20 u/ml
|
Neurobasal |
Fasano, C., Thibault, D. and Trudeau, L.: Culture of Postnatal Mesencephalic Dopamine Neurons on an Astrocyte Monolayer,Current Protocols in Neuroscience Vol. 44,,
,
3.21.1, 2008 |
Mouse
|
Mouse, embryonic and postnatal |
Cortical neurons |
Papain: 4-10 u/ml
|
Neurobasal |
Hilgenberg, L. and Smith, M.: Preparation of Dissociated Mouse Cortical Neuron Cultures., J Vis Exp
10,
562, 2007 |
Mouse
|
Mouse |
Neurosperes |
PDS kit: per instructions
|
DMEM/F12 |
Klein C, Butt SJ, Machold RP, Johnson JE, and Fishell G.: Cerebellum- and forebrain-derived stem cells possess intrinsic regional character, Development
132,
4497, 2005 |
Mouse
|
Mouse, 1 day |
Neural progenitor |
PDS kit: per instructions
|
see reference |
Seaberg, R., Smukler, S. and Van der Kooy, D.: Intrinsic Differences Distinguish Transiently Neurogenic Progenitors from Neural Stem Cells in the Early Postnatal Brain., Dev Biol 278,
71, 2005 |
Mouse
|
Mouse |
Granule cell precursors, pre-neoplastic and tumor cells |
Papain: 10 u/ml
Deoxyribonuclease I: 250 u/ml
|
Neurobasal/B27 |
Oliver, T., Read, T., Kessler, J., Mehmeti, A., Wells, J., Huynh, T., Lin, S. and Wechsler-Reya, R.: Loss of Patched and Disruption of Granule Cell Development in a Pre-Neoplastic Stage of Medulloblastoma., Development
132,
2425, 2005 |
Mouse
|
Mouse |
Cortical neurons |
PDS kit: per instructions
|
Neurobasal |
Hernandez, F., Perez, M., Lucas, J., Mata, A., Bhat, R. and Avila, J.: Glycogen Synthase Kinase-3 plays a Crucial Role in Tau Exon 10 Splicing and Intranuclear Distribution of SC35. Implications for Alzheimer's Disease., J Biol Chem 279,
3801, 2004 |
Mouse
|
Mouse, 3 day |
Microglia |
Papain: 90 u/ml
Deoxyribonuclease I: 2000 u/ml
|
Eagle's MEM |
Nishioku T, Hashimoto K, Yamashita K, Liou SY, Kagamiishi Y, Maegawa H, Katsube N, Peters C, von Figura K, Saftig P, Katunuma N, Yamamoto K, Nakanishi H: Involvement of cathepsin E in exogenous antigen processing in primary cultured murine microglia, J Biol Chem 277,
4816, 2002 |
Mouse
|
Mouse, SD, 8-12 week |
Microglia |
Trypsin: 0.125%
Collagenase Type 2: 0.01%
Deoxyribonuclease I: .005%
|
RPMI-1640 |
O'Donnell SL, Frederick TJ, Krady JK, Vannucci SJ, Wood TL: IGF-I and microglia/macrophage proliferation in the ischemic mouse brain, Glia 39,
85, 2002 |
Mouse
|
Mouse, 2-5 day |
Postnal substantia nigra |
PDS kit: per instructions
|
see reference |
Smeyne Michelle, Smeyne RichardJ: Method for culturing postnatal substantia nigra as an in vitro model of experimental Parkinson's disease, Brain Res Brain Res Protoc 9,
105-11, 2002 |
Mouse
|
Mouse, embryonic |
Cortical progenitors |
PDS kit: per instructions
|
Serum free medium |
Estivill-Torrus, G., Pearson, H., Van Heyningen, V., Price, D. and Rashbass, P.: Pax6 is Required to Regulate the Cell Cycle and the Rate of Progression from Symmetrical to Asymmetrical Division in Mammalian Cortical Progenitors., Development
129,
455, 2002 |
Mouse
|
Mouse, 1 day |
Neurons and glia |
PDS kit: per instructions
|
Neurobasal |
Martin-Aparicio, E., Yamamoto, A., Hernandez, F., Hen, R., Avila, J. and Lucas, J.: Proteasomal-Dependent Aggregate Reversal and Absence of Cell Death in a Conditional Mouse Model of Huntington's Disease., J Neurosci 21,
8772, 2001 |
Mouse
|
Mouse |
Cerebellar granule cell precursors |
Papain: 0.435%
Deoxyribonuclease I: 0.05%
|
EBSS |
Miyazawa K, Himi T, Garcia V, Yamagishi H, Sato S, and Ishizaki Y.: A role for p27/Kip1 in the control of cerebellar granule cell precursor proliferation, J Neurosci 20,
5756, 2000 |
Mouse
|
Mouse |
Astrocytes |
Trypsin: 0.25%
|
DMEM |
Lim, D, and Alvarez-Buylla, A.: Interaction Between Astrocytes and Adult Subventricular Zone Precursors Stimulates Neurogenesis, Proc Natl Acad Sci U S A 96,
7526, 1999 |
Mouse
|
Mouse, newborn |
Hippocampal cells |
Papain: 10 u/ml
|
DMKM |
Jun K, Choi G, Yang SG, Choi KY, Kim H, Chan GC, Storm DR, Albert C, Mayr GW, Lee CJ, Shin HS: Enhanced hippocampal CA1 LTP but normal spatial learning in inositol 1,4,5-trisphosphate 3-kinase(A)-deficient mice, Learn Mem
5,
317-30, 1998 |
Mouse
|
Mouse, SWR or CF1, 1-3 months |
Papillae, taste receptor |
Pronase E: 0.15%
|
Carbonate-Phosphate buffer (see reference) |
Spielman, A., Mody, I., Brand, J., Whitney, G., MacDonald, J., and Salter, M.: A Method for Isolating and Patch-Clamping Single Mammalian Taste Receptor Cells, Brain Res 503,
326, 1989 |
Mouse
|
Mouse, 0-30 day |
Neural |
Trypsin NF 1:250: 0.25%
|
BSS |
Shrier, B., Wilson, S., and Nirenberg, M.: Cultured Cell Systems and Methods for Neurobiology, Vol. 32,,
,
765, 1974 |
Ovine
|
Ovine |
Neurons |
Papain: 0.2%
Deoxyribonuclease I: 0.1%
|
Hibernate |
Reddy, R., Amodei, R., Estill, C., Stormshak, F., Meaker, M. and Roselli, C.: Effect of Testosterone on Neuronal Morphology and Neuritic Growth of Fetal Lamb Hypothalamus-Preoptic Area and Cerebral Cortex in Primary Culture., PLoS ONE
10,
e0129521, 2015 |
Ovine
|
Ovine |
Neurons |
PDS kit: per instructions
|
DMEM |
Lepore G, Gadau S, Mura A, Zedda M, Farina V: Aromatase Immunoreactivity in Fetal Ovine Neuronal Cell Cultures Exposed to Oxidative Injury., Eur J Histochem 53,
e28, 2009 |
Ovine
|
Lamb (also calf) |
Oligodendroglia Neural |
Trypsin: 0.1%
|
(see reference) |
Poduslo, S., Miller, K., and McKhann, G.: Metabolic Properties of Maintained Oligodendroglia Purified from Brain, J Biol Chem 253,
1592, 1978 |
Porcine
|
Mini pigs, Yucatan (Susscrofa Yucatan), 4-6 months |
Microvascular |
Collagenase: 0.1%
|
HBSS |
Robinson, D.H., Kang,Y., Deschner, S.H., and Nielsen, T.B.: Morphologic Plasticity and Periodicity : Porcine Cerebral Microvascular Cells in Culture, In Vitro Cell Dev Biol 26,
169, 1990 |
Rat
|
Rat, SD, 1-2 day |
Oligodendrocyte precursor cells |
Papain: 16.5 u/ml
|
HBSS |
Kurachi, M., Mikuni, M. and Ishizaki, Y.: Extracellular Vesicles from Vascular Endothelial Cells Promote Survival, Proliferation and Motility of Oligodendrocyte Precursor Cells., PLoS ONE
11,
e0159158, 2016 |
Rat
|
Rat, SD, E18 |
Neurons |
Papain: 20 u/ml
|
HBSS |
Folts, C., Scott-Hewitt, N., Proschel, C., Mayer-Proschel, M. and Noble, M.: Lysosomal Re-acidification Prevents Lysosphingolipid-Induced Lysosomal Impairment and Cellular Toxicity., PLoS Biol
14,
e1002583, 2016 |
Rat
|
Rat, P0-P15 |
Astrocytes |
Papain: 5 u/ml
Deoxyribonuclease I: 0.004%
|
DPBS |
Foo, L.: Purification of Rat and Mouse Astrocytes by Immunopanning., Cold Spring Harb. Protoc. 2013,
421-32, 2013 |
Rat
|
Rat, SD, neonatal |
Neurons |
Papain: 20 u/ml
|
Neuro medium |
Kaiser, O., Aliuos, P., Wissel, K., Lenarz, T., Werner, D., Reuter, G., Kral, A. and Warnecke, A.: Dissociated Neurons and Glial Cells Derived from Rat Inferior Colliculi After Digestion with Papain., PLoS ONE
8,
e80490, 2013 |
Rat
|
Rat, SD, 19-21 day |
Suprachiasmatic nucleus neurons |
Papain: 100 u/ml
|
MEM |
Cao, R., Li, A., Cho, H., Lee, B. and Obrietan, K.: Mammalian Target of Rapamycin Signaling Modulates Photic Entrainment of the Suprachiasmatic Circadian Clock., J Neurosci 30,
6302, 2010 |
Rat
|
Rat, Fisher, 7-21 month |
Hippocampal neurons |
Papain: 0.2%
|
Hibernate A |
Chen, N., Newcomb, J., Garbuzova-Davis, S., Davis Sanberg, C., Sanberg, P. and Willing, A.: Human Umbilical Cord Blood Cells Have Trophic Effects on Young and Aging Hippocampal Neurons in Vitro., Aging Dis 1,
173, 2010 |
Rat
|
Rat, SD, 7 day |
Cerebellar granule neurons |
PDS kit: per instructions
|
PBS |
Tanaka, S., Shaikh, I., Chiocca, E. and Saeki, Y.: The Gs-Linked Receptor GPR3 Inhibits the Proliferation of Cerebellar Granule Cells During Postnatal Development., PLoS ONE
4,
e5922, 2009 |
Rat
|
Rat, neonatal |
Astrocytes |
Papain: 20 u/ml
|
EBSS |
Shigetomi, E. and Khakh, B.: Measuring Near Plasma Membrane and Global Intracellular Calcium Dynamics in Astrocytes., J Vis Exp
26,
1142, 2009 |
Rat
|
Rat, neonatal |
Hippocampal neurons |
Papain: 10 u/ml
|
EBSS |
Richler Esther, Chaumont Severine, Shigetomi Eiji, Sagasti Alvaro, Khakh Baljit S: Tracking transmitter-gated P2X cation channel activation in vitro and in vivo, Nat Methods
5,
87-93, 2008 |
Rat
|
Rat, SD |
Hippocampal |
Trypsin: 0.1%
|
Neurobasal |
Akanda, N., Tofighi, R., Brask, J., Tamm, C., Elinder, F. and Ceccatelli, S.: Voltage-Dependent Anion Channels (VDAC) in the Plasma Membrane Play a Critical Role in Apoptosis in Differentiated Hippocampal Neurons but not in Neural Stem Cells., Cell Cycle 7,
3225, 2008 |
Rat
|
Rat, neonatal |
Dura mater |
Collagenase Type 1: 0.075%
|
DMEM |
Peptan, I., Hong, L. and Evans, C.: Multiple Differentiation Potentials of Neonatal Dura Mater-Derived Cells., Neurosurgery
60,
346, 2007 |
Rat
|
Rat, E18 |
Hippocampal neurons |
Papain: 0.2%
|
Hibernate |
Jekabsons MB, Nicholls DG: Bioenergetic analysis of cerebellar granule neurons undergoing apoptosis by potassium/serum deprivation, Cell Death Differ
13,
1595-610, 2006 |
Rat
|
Rat, Wistar, 1-3 day |
Hippocampal neurons |
Trypsin: 0.05%
|
DMEM |
Velasco Myrian, Garcia Esperanza, Onetti Carlos G: Glucose deprivation activates diversity of potassium channels in cultured rat hippocampal neurons, Cell Mol Neurobiol 26,
307-19, 2006 |
Rat
|
Rat, E19 |
Hippocampal neuron |
Papain: 10 u/ml
|
MEM |
Khakh Baljit S, Fisher James A, Nashmi Raad, Bowser David N, Lester Henry A: An angstrom scale interaction between plasma membrane ATP-gated P2X2 and alpha4beta2 nicotinic channels measured with fluorescence resonance energy transfer and total internal reflection fluorescence microscopy, J Neurosci 25,
6911-20, 2005 |
Rat
|
Rat, Wistar, 14 day |
Visual cortical |
PDS kit: per instructions
|
EBSS |
Mizoguchi Y, Kanematsu T, Hirata M, Nabekura J: A rapid increase in the total number of cell surface functional GABAA receptors induced by brain-derived neurotrophic factor in rat visual cortex, J Biol Chem 278,
44097, 2003 |
Rat
|
Rat, Wistar, male |
Cerebral endothelial |
Collagenase Type 3: 0.2%
|
MEM |
Floris S, Van den Born J, van der Pol SM, Dijkstra CD, De Vries HE: Heparan sulfate proteoglycans modulate monocyte migration across cerebral endothelium, J Neuropathol Exp Neurol 62,
780, 2003 |
Rat
|
Rat, SD, E18 |
Cortical cells |
Papain: 20 u/ml
Deoxyribonuclease I: .005%
|
EBSS |
Behar, T, Smith, S, Kennedy, R, Mckenzie, J, Maric, I and Barker, J: GABA(B) receptors mediate motility signals for migrating embryonic cortical cells, Cereb Cortex 11,
744-53, 2001 |
Rat
|
Rat, wistar, 250-300 g |
Cerebral artery smooth muscle cells |
Papain: 1.5 mg/ml
Collagenase Type 4: 1.5 mg/ml
|
Physiological Salt Solution |
Brzezinska AK, Gebremedhin D, Chilian WM, Kalyanaraman B, Elliott SJ.: Peroxynitrite reversibly inhibits Ca(2+)-activated K(+) channels in rat cerebral artery smooth muscle cells, Am J Physiol Heart Circ Physiol
278,
H1883, 2000 |
Rat
|
Rat, SD, E16 |
Cortical neurons |
PDS kit: with modifications
|
Neurobasal Medium |
Varney MA, Cosford ND, Jachec C, Rao SP, Sacaan A, Lin FF, Bleicher L, Santori EM, Flor PJ, Allgeier H, Gasparini F, Kuhn R, Hess SD, Velicelebi G, Johnson EC: SIB-1757 and SIB-1893: selective, noncompetitive antagonists of metabotropic glutamate receptor type 5, J Pharmacol Exp Ther 290,
170, 1999 |
Rat
|
Rat, wistar, neonatal |
Hippocampal cells |
Papain: 1 mg/ml
|
DMEM |
Boehm S, Betz H: Somatostatin inhibits excitatory transmission at rat hippocampal synapses via presynaptic receptors, J Neurosci 17,
4066, 1997 |
Rat
|
Rat, SD, Fisher |
Hippocampal neurons |
Papain: 0.2%
|
HibernateA/B27 |
Brewer, G.J.: Isolation and culture of adult rat hippocampal neurons of any age, J Neurosci Methods 71,
143, 1997 |
Rat
|
Rat, E18 |
Hippocampal and cortical neurons |
PDS kit: per instructions
|
Neurobasal media |
Naeve GS, Ramakrishnan M, Kramer R, Hevroni D, Citri Y, Theill LE: Neuritin: a gene induced by neural activity and neurotrophins that promotes neuritogenesis, Proc Natl Acad Sci U S A 94,
2648-53, 1997 |
Rat
|
Rat, SD, newborn |
Glial |
Trypsin: 0.0625%
|
MEM, sterile |
Pixley, S.K.: The Olfactory Nerve Contains Two Populations of Glia, In Vitro 5,
269, 1992 |
Rat
|
Rat, newborn |
Astrocytes |
Trypsin: 0.25%
|
DMEM, HBSS |
Holzwarth, J., Glaum, S., and Miller, R.: Activation of Endothelin Receptors by Sarafotoxin Regulates Ca2+ Homeostasis in Cerebellar Astrocytes, Glia 5,
239, 1992 |
Rat
|
Rat, 60-72 hours old |
Fibroblasts |
Trypsin: 0.2%
|
HEPES buffered DMEM |
Acheson, A., Barker, P., Alderson, R., Miller, F., and Murphy, R.: Detection of Brain-Derived Neurotrophic Factor-like Activity in Fibroblasts and Schwann Cells: Inhibition by Antibodies to NGF, Neuron 7,
265, 1991 |
Rat
|
Rat, SD, adult |
Endothelial |
Collagenase Type 2: 0.5%
|
Medium 199 |
Doron, D., Jacobowitz, D., Heldman, E., Feurerstein, G., Pollard, H., and Hallenbeck, J.: Extracellular Matrix Permits the Expression of Von Willebrand's Factor, Uptake of Di-I-Acetylated Low Density Lipoprotein and Secretion of Prostacyclin in Cultures of Endothelial Cells from Rat Brain Microvessels, In Vitro Cell Dev Biol 27A,
689, 1991 |
Rat
|
Rat, SD, 5 day |
Hippocampal neurons |
Trypsin: 0.2%
Deoxyribonuclease I: 10 ug/ml
|
HBSS |
Mattson, M., and Kater, S.: Development and Selective Neurodegeneration in Cell Cultures From Different Hippocampal Regions, Brain Res 490,
110, 1989 |
Rat
|
Rat, SD, female |
Fetal rat brain |
Collagenase Type 4: 0.1%
|
DMEM |
Matsuda, M.: Serum Proteins Enhance Aggregate Formation of Dissociated Fetal Rat Brain Cells in an Aggregating Culture, In Vitro Cell Dev Biol 24 (10),
1031, 1988 |
Rat
|
Rat, albino, adult and newborn |
Cerebral cortices |
Trypsin: 0.25%
|
PBS |
Giulian, D. and Baker, T.J.: Characterization of Ameboid Microglia Isolated From Developing Mammalian Brain, J Neurosci 6,
2163, 1986 |
Rat
|
Rat, Wistar, newborn |
Germinal matrix |
Trypsin: 0.25%
|
HBSS |
Goldman, J.E., Geier, S.S., and Hirano, M.: Differentiation of Astrocytes and Oligodendrocytes From Germinal Matrix Cells in Primary Culture, J Neurosci 6,
52, 1986 |
Rat
|
Rat, fetus, 18-20 day |
Hippocampal neurons |
Trypsin: 0.25%
|
HBSS, CMF |
Bartlett, W. and Banker, G.: An Electron Microscopic Study of the Development of Axons and Dendrites by Hippocampal Neurons in Culture. I. Cells Which Develop Without Intercellular Contacts, J Neurosci 4,
1944, 1984 |
Rat
|
Rat, fetus, 18 day |
Hippocampi |
Trypsin: 0.1%
|
HBSS |
Rothman, S.: Synaptic Release of Excitatory Amino Acid Neurotransmitter Mediates Anoxic Neuronal Death, J Neurosci 4 (7),
1884, 1984 |
Rat
|
Rat, SD, 19-20 days pregnant |
Neural |
Trypsin: 0.25%
|
DMEM |
Ahmed, Z., Walker, P., and Fellows, R.: Properties of Neurons from Dissociated Fetal Rat Brain in Serum-Free Culture, J Neurosci 3 (12),
2448, 1983 |
Rat
|
Rat, SD, fetal |
Cerebral cortex Hypothalmus |
Deoxyribonuclease I: 0.001%
|
HEPES |
Peterfreund, R. and Vale, W.: High Molecular Weight Somatostatin Secretion by Cultured Rat Brain Cell, Brain Res 239,
463, 1982 |
Rat
|
Rat, Wistar-Kyoto, male, 100 - 200 g |
Endothelial Cerebral |
Collagenase Type 2: 0.05%
|
HBSS |
Diglio, C.A., Grammas, P., Filiberto Giacomelli, M.S., and Wiener, J.: Primary Culture of Rat Cerebral Microvascular Endothelial Cells, Lab Invest 46,
554, 1982 |
Rat
|
Rat, fetus |
Cortical |
Trypsin: 0.25%
|
Puck's D1 (see reference) |
Swaiman, K., Neale, E., Fitzgerald, S., and Nelson, P.: A Method for Large-scale Production of Mouse Brain Cortical Cultures, Brain Res 255,
361, 1982 |
Rat
|
Rat, Wistar, pregnant |
Glial |
Trypsin: 0.05%
|
Eagle's MEM/DMEM |
Abney, E., Bartlett, P., and Raff, M.: Astrocytes, Ependymal Cells, and Oligodendrocytes Develop on Schedule in Dissociated Cell Cultures of Embryonic Rat Brain, Dev Biol 83,
301, 1981 |
Rat
|
Rat, SD, one month old |
Capillary endothelium Pericytes |
Neutral Protease: 0.1%
|
Medium 199 |
Bowman, P., Betz, A., Ar, D., Wolinsky, J., Penney, J., Shivers, R., and Goldstein, G.: Primary Culture of Capillary Endothelium From Rat Brain, In Vitro 17 (4),
353, 1981 |
Rat
|
Rat, Wistar, male, 300-500 g |
Microvessels Endothelial |
Collagenase Type 2: 0.75%
|
Ringers-HEPES buffer |
Williams, S., Gillis, J., Matthews, M., Wagner, R., and Bitensky, M.: Isolation and Characterization of Brain Endothelial Cells: Morphology and Enzyme Activity, J Neurochem 35 (2),
374, 1980 |
Rat
|
Rat, Wistar, adult, 170 g |
Endothelial |
Trypsin: 0.5%
|
BSS |
Phillips, P., Kumar, P., Kumar, S., and Waghe, M.: Isolation And Characterization of Endothelial Cells From Rat And Cow Brain White Matter, J Anat 129,
261, 1979 |
Rat
|
Rat, Holtzmann, 18 day |
Hippocampal neurons |
Trypsin: 0.1%
|
HBSS |
Banker, G., and Cowan, M.: Rat Hippocampal Neurons in Dispersed Cell Culture, Brain Res 126,
397, 1977 |
Rat
|
Rat, SD, 2 days |
Pineal |
Trypsin: 0.25%
|
DMEM, MEM |
Rowe, V., Neale, E., Avins, L., Guroff, G., and Schrier, B.: Pineal gland cells in culture. Morphology, Biochemistry, Differentiation, and co-culture with sympathetic neurons, Exp Cell Res
104,
345, 1977 |
Shellfish
|
Helisoma trivolvis |
Neurons Buccal ganglia |
Trypsin: 0.2%
|
Saline, Sterile |
Hadley, R.D., Bodnar, D.A., and Kater, S.B.: Formation of Electrical Synapses Between Isolated, Cultured Helisoma Neurons Requires Mutual Neurite Elongation, J Neurosci 5,
3145, 1985 |
|
|