Cartilage - Tissue Tables
Species | Species Detail | Cells | Enzyme(s) | Medium |
Reference |
Bovine
|
Bovine, 18-36 month |
Chondrocytes |
Collagenase Type 1: 0.1%
|
DMEM |
White, R. and Gibson, J.: The Effect of Oxygen Tension on Calcium Homeostasis in Bovine Articular Chondrocytes., J Orthop Surg Res
5,
27, 2010 |
Bovine
|
Bovine, 6-8 week |
Chondrocytes |
Collagenase Type 2: 0.2%
|
DMEM |
Hwang, Y., Sangaj, N. and Varghese, S.: Interconnected Macroporous Poly(ethylene glycol) Cryogels as a Cell Scaffold for Cartilage Tissue Engineering., Tissue Eng Part A
16,
3033-41, 2010 |
Bovine
|
Bovine, 1-3 month |
Chondrocytes |
Collagenase Type 4: 390 u/ml
|
DMEM |
Lima, E., Tan, A., Tai, T., Marra, K., DeFail, A., Ateshian, G. and Hung, C.: Genipin Enhances the Mechanical Properties of Tissue-Engineered Cartilage and Protects Against Inflammatory Degradation When Used as a Medium Supplement., J Biomed Mater Res 91,
692, 2009 |
Bovine
|
Steers, 1-2 years |
Chondrocytes |
Trypsin: 0.20%
|
HBSS |
Mackintosh, D., and Mason, R.: Pharmacological Actions of 17 Beta-oestradiol on Articular Cartilage Chondrocytes and Chondrosarcoma Chondrocytes in the Absence of Oestrogen Receptors, Biochim Biophys Acta 964,
295, 1988 |
Bovine
|
Calf, 1-14 days |
Chondrocytes |
Collagenase Type 2: 0.20%
|
PBS |
Klagsbrun, M.: Large Scale Preparation of Chondrocytes,Methods of Enzymology Vol. 58,,
,
560, 1979 |
Canine
|
Canine |
Chondrocytes |
Collagenase Type 2: 0.3%
Trypsin: 0.25%
|
Ham's F12 |
Lee, J., Kim, H., Kim, J., Bae, S., Joo, D., Huh, K., Fang, Y., Jeong, J., Kim, M. and Kim, Y.: Microencapsulation of Pancreatic Islets with Canine Ear Cartilage for Immunoisolation., Transplant Proc 44,
1091-4, 2012 |
Chicken
|
Chick embryos, HH stage |
Mesenchymal |
Trypsin: 0.1%
|
DMEM (see reference) |
Wong, M., and Tuan, R.: Nuserum, A Synthetic Serum Replacement, Supports Chondrogenesis of Embryonic Chick Limb Bud Mesenchymal Cells in Micromass Culture, In Vitro Cell Dev Biol 29A,
917, 1993 |
Chicken
|
Chicken, broiler strain, 8-10 weeks |
Matrix vesicles Epiphyseal growth plate |
Trypsin: 0.1%
|
Tris-buffered saline |
Genge, B., Wu, L. and Wuthier, R.: Differential Fractionation of Matrix Vesicle Proteins: Further Characterization of the Acidic Phospholipid-dependent Ca2+-Binding Proteins, J Biol Chem 265,
4703, 1990 |
Chicken
|
Chicken, broiler strain, 8-10 week |
Matrix vesicles |
Trypsin: 0.1%
|
(see reference) |
Genge, B.R., Wu, L.N.Y., and Wuthier, R.E.: Identification of Phospholipid-dependent Calcium-binding Proteins as Constituents of Matrix Vesicles, J Biol Chem 264,
10917, 1989 |
Chicken
|
Chick embryos, White Leghorn, 19 day old |
Fibroblasts Epithelial-like |
Trypsin: 0.25%
|
E 199 medium |
Gionti, E., Capasso, O., and Cancedda, R.: The Culture of Chick Embryo Chondrocytes and the Control of Their Differentiated Functions in Vitro, J Biol Chem 258 (11),
7190, 1983 |
Chicken
|
Chick embryos, 19 day old |
Chondrocytes |
Trypsin: 0.75%
|
Coon's modified F-12 |
Capasso, O., Gionti, E., Pontarelli, G., Ambesi-Impiobato, F., Nitsch, L., Tajana, G., and Cancedda, R.: The Culture of Chick Embryo Chondrocytes and the Control of Their Differentiated Functions In Vitro, Exp Cell Res
142,
197, 1982 |
Chicken
|
Chick embryos, White Leghorn |
Wing buds |
Trypsin: 0.1%
|
Saline G |
Ahrens, P., Solursh, M., and Reiter, R.: Stage-Related Capacity for Limb Chondrogenesis in Cell Culture, Dev Biol 60,
69, 1977 |
Equine
|
Equine |
Chondrocytes |
Collagenase Type 2: 0.15%
|
DMEM |
Visser, J., Levett, P., te Moller, N., Besems, J., Boere, K., van Rijen, M., de Grauw, J., Dhert, W., van Weeren, P. and Malda, J.: Crosslinkable Hydrogels Derived from Cartilage, Meniscus, and Tendon Tissue., Tissue Eng Part A
21,
1195-206, 2015 |
Equine
|
Equine |
Chondrocytes |
Collagenase Type 2: 0.08%
|
DMEM/F12 |
Ley, C., Svala, E., Nilton, A., Lindahl, A., Eloranta, M., Ekman, S. and Skioldebrand, E.: Effects of High Mobility Group Box Protein-1, Interleukin-1B, and Interleukin-6 on Cartilage Matrix Metabolism in Three-Dimensional Equine Chondrocyte Cultures., Conn Tissue Res 52,
290-300, 2011 |
Goat
|
Goat |
Chondrocytes |
Collagenase Type 2: 2%
|
DMEM |
Bekkers,J, Creemers, L, Tsuchida, A, van Rijen, M, Custers, R, Dhert, W and Saris, D.: One-Stage Focal Cartilage Defect Treatment with Bone Marrow Mononuclear Cells and Chondrocytes Leads to Better Macroscopic Cartilage Regeneration Compared to Microfracture in Goats., Osteoarthritis Cartilage
21,
950-6, 2013 |
Human
|
Human |
Chondrocytes |
Trypsin: 0.25%
Hyaluronidase: 0.1%
Collagenase Type 2: 1000 u/ml
Collagenase Type 1: 400 u/ml
|
Ham's F12 |
Muraglia, A., Nguyen, V., Nardini, M., Mogni, M., Coviello, D., Dozin, B., Strada, P., Baldelli, I., Formica, M., Cancedda, R. and Mastrogiacomo, M.: Culture Medium Supplements Derived from Human Platelet and Plasma: Cell Commitment and Proliferation Support., Front Bioeng Biotechnol
5,
66, 2017 |
Human
|
Human |
Chondrocytes |
Collagenase Type 2: 0.15%
|
DMEM |
Andrews, S., Kunze, M., Mulet-Sierra, A., Williams, L., Ansari, K., Osswald, M. and Adesida, A.: Strategies to Mitigate Variability in Engineering Human Nasal Cartilage., Sci Rep
7,
6490, 2017 |
Human
|
Human |
Chondrocytes |
Collagenase Type 2: 0.15%
|
DMEM |
Capsoni, F., Ongari, A., Lonati, C., Accetta, R., Gatti, S. and Catania, A.: Alpha-Melanocyte-Stimulating-Hormone (a-MSH) Modulates Human Chondrocyte Activation Induced by Proinflammatory Cytokines., BMC Musculoskelet Disord
16,
154, 2015 |
Human
|
Human |
Chondrocytes |
Collagenase: 0.3%
|
DMEM |
Tamai, M., Nagasao, T., Yanaga, H., Hamamoto, Y., Kogure, T. and Tanaka, Y.: Correction of Secondary Deformity after Nuss Procedure for Pectus Excavatum by Means of Cultured autologous Cartilage Cell Injection., Int J Surg Case Rep
15,
70-3, 2015 |
Human
|
Human, 52-82 years |
Chondrocytes |
Collagenase Type 2: 0.2%
Pronase: 0.15%
|
DMEM/F12 |
Pallu, S., Francin, P., Guillaume, C., Gegout-Pottie, P., Netter, P., Mainard, D., Terlain, B. and Presle, N.: Obesity Affects the Chondrocyte Responsiveness to Leptin in Patients with Osteoarthritis., Arthritis Res Ther 12,
R112, 2010 |
Human
|
Human |
Synoviocytes |
Collagenase Type 1: 0.4%
|
DMEM |
Kim Wan-Uk, Kwok Seung-Ki, Hong Kyung-Hee, Yoo Seung-Ah, Kong Jin-Sun, Choe Jongseon, Cho Chul-Soo: Soluble Fas ligand inhibits angiogenesis in rheumatoid arthritis, Arthritis Res Ther 9,
R42, 2007 |
Human
|
Human, 26-68 year |
Meniscus and cartilage |
Collagenase Type 2: 0.15%
|
DMEM |
Marsano A, Millward-Sadler SJ, Salter DM, Adesida A, Hardingham T, Tognana E, Kon E, Chiari-Grisar C, Nehrer S, Jakob M, Martin I: Differential cartilaginous tissue formation by human synovial membrane, fat pad, meniscus cells and articular chondrocytes, Osteoarthritis Cartilage
15,
48-58, 2007 |
Human
|
Human, 64-83 yr |
Chondrocytes |
Collagenase Type 2: 0.08%
|
DMEM/F12 |
Tallheden T, Bengtsson C, Brantsing C, Sjogren-Jansson E, Carlsson L, Peterson L, Brittberg M, and Lindahl A.: Proliferation and Differentiation Potential of Chondrocytes from Osteoarthritic Patients, Arthritis Res Ther 7(3),
R560, 2005 |
Human
|
Human, adult |
Synovial |
Collagenase: 0.15%
Hyaluronidase: 0.1%
Deoxyribonuclease I: 0.015%
|
DMEM |
Liagre B, Vergne-Salle P, Corbiere C, Charissoux JL, and Beneytout JL.: Diosgenin, a Plant Steroid, Induces Apoptosis in Human Rheumatoid Arthritis Synoviocytes with Cyclooxygenase-2 Overexpression, Arthritis Res Ther 6(4),
R373, 2004 |
Human
|
Human, adult |
Chondrocytes |
Collagenase Type 2: 0.15%
|
DMEM |
Jakob M, Demarteau O, Schafer D, Stumm M, Heberer M, and Martin I.: Enzymatic Digestion of Adult Human Articular Cartilage Yields a Small Fraction of the Total Available Cells, Conn Tissue Res 44,
173, 2003 |
Human
|
Human, adults |
Synoviocytes |
Collagenase Type 1: 0.1%
|
RPMI |
McEvoy A., Murphy E., Ponnio T., Conneely O., Bresnihan B., FitzGerald O., and Murphy E.: Activation of Nuclear Orphan Receptor NURR1 Transcription by NF-kappa B and Cyclic Adenosine 5'-Monophosphate Response Element-binding Protein in Rheumatoid Arthritis Synovial Tissue, J Immunol 168(6),
2979, 2002 |
Human
|
Human, 15-60 years |
Chondrocytes |
Collagenase Type 2: 0.2%
|
DMEM |
Rotter N, Bonassar LJ, Tobias G, Lebl M, Roy AK, Vacanti CA: Age dependence of cellular properties of human septal cartilage: implications for tissue engineering, Arch Otolaryngol Head Neck Surg 127,
1248-52, 2001 |
Human
|
Human |
Synovial fibroblasts |
Collagenase: 0.4%
|
DMEM |
Sarkissian M, Lafyatis R: Integrin engagement regulates proliferation and collagenase expression of rheumatoid synovial fibroblasts, J Immunol 162,
1772-9, 1999 |
Human
|
Human |
Septal chondrocytes |
Collagenase Type 2: 0.2%
Hyaluronidase: 0.01%
Deoxyribonuclease I: 0.015%
|
DMEM/F12 |
Dunham B P, Koch R J: Basic fibroblast growth factor and insulinlike growth factor I support the growth of human septal chondrocytes in a serum-free environment, Arch Otolaryngol Head Neck Surg 124,
1325-30, 1998 |
Human
|
Human w/ rheumatoid arthritis |
Synovial tissue |
Trypsin: 0.05%
|
DMEM (see reference) |
Dayer, J., Krane, S., Russell, R., and Robinson, D.: Production of Collagenase and Prostaglandins by Isolated Adherent Rheumatoid Synovial Cells, Proc Natl Acad Sci U S A 73 (3),
945, 1976 |
Human
|
Human, 13-62 years |
Articular chondrocytes |
Trypsin: 0.2%
|
BSS |
Srivastava, V.M.L., Malemud, C.J., Hough, A.J., Bland, J.H., and Sokoloff, L.: Preliminary Experience with Cell Culture of Human Articular Chondrocytes, Arthritis Rheum 17,
165, 1974 |
Human
|
Human, 26-84 years |
Chondrocytes |
Collagenase:
|
GBSS |
Manning, W.K., and Bonner, W.M.: Isolation and Culture of Chondrocytes From Human Adult Articular Cartilage, Arthritis Rheum 10,
235, 1967 |
Mouse
|
Mouse, 2-5 day |
Chondrocytes |
Trypsin: 0.25%
Collagenase Type 1: 86.5 u/ml
|
DMEM |
Otsuru, S., Hofmann, T., Raman, P., Olson, T., Guess, A., Dominici, M. and Horwitz, E.: Genomic and Functional Comparison of Mesenchymal Stromal Cells Prepared Using Two Isolation Methods., Cytotherapy
17,
262-70, 2015 |
Mouse
|
Mouse, 1 day |
Chondrocytes |
Collagenase: 0.2%
|
DMEM |
Terpstra, L, Prud'homme, J, Arabian, A, Takeda, S, Karsenty, G, Dedhar, S, and St-Arnaud, R.: Reduced Chondrocyte Proliferation and Chondrodysplasia in Mice Lacking the Integrin-linked Kinase in Chondrocytes, J Cell Biol
162,
139, 2003 |
Ovine
|
Sheep, 2 month |
Chondrocytes |
Collagenase Type 2: 0.3%
|
Ham's F-12 |
Kojima Koji, Bonassar Lawrence J, Roy Amit K, Mizuno Hirokazu, Cortiella Joaquin, Vacanti Charles A: A Composite Tissue-Engineered Trachea Using Sheep Nasal Chondrocyte and Epithelial Cells, FASEB J
17,
823-8, 2003 |
Porcine
|
Porcine, 1 year |
Chondrocytes |
Collagenase Type 1: 0.2%
|
DEMEM |
Chowdhury, T., Schulz, R., Rai, S., Thuemmler, C., Wuestneck, N., Bader, A and Homandberg, G: Biomechanical Modulation of Collagen Fragment-Induced Anabolic and Catabolic Activities in Chondrocyte/Agarose Constructs., Arthritis Res Ther 12,
R82, 2010 |
Porcine
|
Porcine, 2-4 month |
Chondrons |
Neutral Protease: 0.3%
Collagenase: 0.2%
|
PBS |
Graff RD, Lazarowski ER, Banes AJ, Lee GM: ATP release by mechanically loaded porcine chondrons in pellet culture, Arthritis Rheum 43,
1571-9, 2000 |
Rabbit
|
Rabbit, New Zealand, 1.2-1.4 kg |
Chondrocytes |
Collagenase Type 2: 0.025%
Pronase: 0.2%
|
DMEM |
Ju, X., Deng, M., Ao, Y., Yu, C., Wang, J., Yu, J., Cui, G. and Hu, Y.: Protective Effect of Sinomenine on Cartilage Degradation and Chondrocytes Apoptosis., Yakugaku Zasshi
130,
1053-60, 2010 |
Rabbit
|
Rabbit, New Zealand, 1.8-2.3kg |
Chondrocytes |
Hyaluronidase: .05%
Collagenase Type 2: 0.2%
Trypsin: 0.2%
|
Gey's solution |
Mehraban F, Tindal MH, Proffitt MM, Moskowitz RW.: Temporal pattern of cysteine endopeptidase (cathepsin B) expression in cartilage and synovium from rabbit knees with experimental osteoarthritis: gene expression in chondrocytes in response to interleukin-1 and matrix depletion, Ann Rheum Dis 56,
108, 1997 |
Rabbit
|
Rabbit, New Zealand, white, 4-6 wk & 22-25 wk |
Chondrocytes |
Protease XIV: 5 mg/g of tissue
|
Ham's F-12 |
Plaas, A., Sandy, J., and Kimura, J.: Biosynthesis of Cartilage Proteoglycan & Link Protein Articular Chondrocytes, J Biol Chem 263,
7560, 1988 |
Rabbit
|
Rabbit, white, male, 8 weeks |
Chondrocytes |
Trypsin: 0.2%
|
Gey's BSS |
Benya, P.D., Padilla, S.R., and Nimni, M.E.: The Progeny of Rabbit Articular Chondrocytes Synthesize Collagen Types I and III and Type I Trimer, but Not Type II, Biochemistry
16,
865, 1977 |
Rabbit
|
Rabbit, New Zealand white or Dutch, 1 week (also human, newborn) |
Chondrocytes |
Trypsin: 0.1%
|
Saline G, CMF |
Schindler, F.H., Ose, M.A., and Solursh, M.: The Synthesis of Cartilage Collagen by Rabbit and Human Chondrocytes in Primary Cell Culture, In Vitro 12,
44, 1976 |
Rabbit
|
Rabbit, New Zealand white, immature, 2.25 - 3.3 Kg |
Articular chondrocytes Hyaline |
Trypsin: 0.2%
|
Gey's BSS |
Green, J.R., and William, T.: Articular Cartilage Repair. Behavior of Rabbit Chondrocytes During Tissue Culture and Subsequent Allografting, Clin Orthop Relat Res
,
237, 1976 |
Rabbit
|
Rabbit, New Zealand white, male, 250-350 g |
Epiphyseal Articular cartilage |
Trypsin: 0.25%
|
Eagle's basal medium |
Bentley, G., and Greer , R.: Homotransplantation of Isolated Epiphyseal and Articular Cartilage Chondrocytes into Joint Surfaces of Rabbits, Nature
230,
385, 1971 |
Rat
|
Rat |
Chondrocytes |
Collagenase Type 2: 0.15%
Trypsin: 0.25%
|
DMEM/F12 |
Mei, L., Shen, B., Ling, P., Liu, S., Xue, J., Liu, F., Shao, H., Chen, J., Ma, A. and Liu, X.: Culture-expanded Allogenic Adipose Tissue-derived Stem Cells Attenuate Cartilage Degeneration in an Experimental Rat Osteoarthritis Model., PLoS ONE
12,
e0176107, 2017 |
Rat
|
Rat, SD, young, 100-120 g |
Chondrocytes |
Trypsin: 0.2%
|
Ham's F-12 medium |
Shimomura, Y., Yoneda, T., and Suzuki, F.: Osteogenesis by Chondrocytes from Growth Cartilage of Rat Rib, Calcif Tissue Res 19,
179, 1975 |
|
|