Colon - Tissue Tables
Species | Species Detail | Cells | Enzyme(s) | Medium |
Reference |
Guinea-Pig
|
Guinea-pig, adult |
Enterochromaffin |
Trypsin: 0.05%
Collagenase Type 1: 0.1%
|
DMEM |
Raghupathi, R., Duffield, M., Zelkas, L., Meedeniya, A., Brookes, S., Sia, T., Wattchow, D., Spencer, N. and Keating, D.: Identification of Unique Release Kinetics of Serotonin From Guinea-pig and Human Enterochromaffin Cells., J Physiol
591,
5959-75, 2013 |
Guinea-Pig
|
Guinea-Pig, male, 200-250g |
Myenteric ganglia |
Collagenase Type 4: 0.2%
Protease: 0.1%
|
Kreb's solution |
Kang, M., Maguma, H., Smith, T., Ross, G., Dewey, W. and Akbarali, H.: The Role of β-arrestin2 in the Mechanism of Morphine Tolerance in the Mouse and Guinea Pig Gastrointestinal Tract., J Pharmacol Exp Ther 340,
567-76, 2012 |
Human
|
Human |
Lamina propria mononuclear |
Collagenase Type 1: 0.1%
Deoxyribonuclease I: 5 u/ml
|
HBSS |
Moser, A., Spindelboeck, W., Strohmaier, H., Enzinger, C., Gattringer, T., Fuchs, S., Fazekas, F., Gorkiewicz, G., Wurm, P., Hogenauer, C. and Khalil, M.: Mucosal Biopsy Shows Immunologic Changes of the Colon in Patients with Early MS, Neurol Neuroimmunol Neuroinflamm
4,
e362, 2017 |
Human
|
Human |
Colon enteric neural |
Neutral Protease: 0.5%
Collagenase animal free: 0.1%
Hyaluronidase: 0.1%
Deoxyribonuclease I: 0.01%
|
Ham's F-12 |
Rollo, B., Zhang, D., Stamp, L., Menheniott, T., Stathopoulos, L., Denham, M., Dottori, M., King, S., Hutson, J. and Newgreen, D.: Enteric Neural Cells from Hirschsprung Disease Patients Form Ganglia in Autologous Aneuronal Colon, Cell Mol Gastroenterol Hepatol 2,
92, 2016 |
Human
|
Human |
Gastrointestinal epithelial |
Collagenase Type 1: 0.2%
|
DMEM/F12 |
VanDussen, K., Marinshaw, J., Shaikh, N., Miyoshi, H., Moon, C., Tarr, P., Ciorba, M. and Stappenbeck, T.: Development of an Enhanced Human Gastrointestinal Epithelial Culture System to Facilitate Patient-Based Assays., Gut 64,
911-20, 2015 |
Human
|
Human |
Colon |
Trypsin: 0.1%
Collagenase: 0.1%
|
HBSS |
Ali, M., Anand, S., Tangella, K., Ramkumar, D. and Saif, T.: Isolation of Primary Human Colon Tumor Cells from Surgical Tissues and Culturing Them Directly on Soft Elastic Substrates for Traction Cytometry., J Vis Exp
,
e52532, 2015 |
Human
|
Human |
Enterochromaffin |
Trypsin: 0.05%
Collagenase Type 1: 0.1%
|
DMEM |
Raghupathi, R., Duffield, M., Zelkas, L., Meedeniya, A., Brookes, S., Sia, T., Wattchow, D., Spencer, N. and Keating, D.: Identification of Unique Release Kinetics of Serotonin From Guinea-pig and Human Enterochromaffin Cells., J Physiol
591,
5959-75, 2013 |
Human
|
Human |
Colonic epithelial |
Collagenase: 150 u/ml
Neutral Protease: 0.04 mg/ml
|
Basal X media |
Roig, A., Eskiocak, U., Hight, S., Kim, S., Delgado, O., Souza, R., Spechler, S., Wright, W. and Shay, J.: Immortalized Epithelial Cells Derived from Human Colon Biopsies Express Stem Cell Markers and Differentiate In Vitro., Gastroenterol 138,
1012, 2010 |
Human
|
Human |
Colorectal cancer |
Collagenase Type 4: 1%
Deoxyribonuclease I: 0.2%
|
HBSS |
Zhou, J., Belov, L., Huang, P., Shin, J., Solomon, M., Chapuis, P., Bokey, L., Chan, C., Clarke, C., Clarke, S. and Christopherson, R.: Surface Antigen Profiling of Colorectal Cancer Using Antibody Microarrays With Fluorescence Multiplexing., J Immunol Methods 355,
40, 2010 |
Human
|
Human |
Colonic epithelial |
Collagenase Type 4: 0.1%
|
not listed |
Huang, E., Hynes, M., Zhang, T., Ginestier, C., Dontu, G., Appelman, H., Fields, J., Wicha, M. and Boman, B.: Aldehyde Dehydrogenase 1 is a Marker for Normal and Malignant Human Colonic Stem Cells (SC) and Tracks SC Overpopulation During Colon Tumorigenesis., Cancer Res 69,
3382-9, 2009 |
Human
|
Human |
Colon cancer |
Collagenase Type 1: 300 u/ml
Hyaluronidase: 100 u/ml
|
DMEM/F12 |
Varnat, F., Duquet, A., Malerba, M., Zbinden, M., Mas, C., Gervaz, P. and Ruiz i Altaba, A.: Human Colon Cancer Epithelial Cells Harbour Active HEDGEHOG-GLI Signalling that is Essential for Tumour Growth, Recurrence, Metastasis and Stem Cell Survival and Expansion., EMBO Mol Med
1,
338-51, 2009 |
Human
|
Human |
Cancer stem cell |
Collagenase Type 3: 200 u/ml
Deoxyribonuclease I: 100 u/ml
|
RPMI-1640 |
Dalerba Piero, Dylla Scott J, Park In-Kyung, Liu Rui, Wang Xinhao, Cho Robert W, Hoey Timothy, Gurney Austin, Huang Emina H, Simeone Diane M, Shelton Andrew A, Parmiani Giorgio, Castelli Chiara, Clarke Michael F: Phenotypic characterization of human colorectal cancer stem cells, Proc Natl Acad Sci U S A 104,
10158-63, 2007 |
Human
|
Human |
Colonic epithelial |
Collagenase:
Neutral Protease: 0.3%
Deoxyribonuclease I: 0.05%
|
RPMI 1640 |
Fukushima, K. and Fiocchi, C.: Paradoxical Decrease of Mitochondrial DNA Deletions in Epithelial Cells of Active Ulcerative Colitis Patients., Am J Physiol Gastrointest Liver Physiol
286,
G804-13, 2004 |
Human
|
Human |
Colonic endothelial cells |
Collagenase Type 2: 0.25%
|
HBSS/5%FBS |
Wang D., Lehman R., Donner D., Matli M., Warren R., and Welton M.: Expression and Endocytosis of VEGF and Its Receptors in Human Colonic Vascular Endothelial Cells, Am J Physiol/Gastro 282,
G1088, 2002 |
Human
|
Human |
Colonocytes |
Collagenase: 0.15%
|
DMEM/F12 |
Emenaker N, Calaf G, Cox D, Basson M and Qureshi N: Short Chain Fatty Acids Differentially Modulate Cellular Phenotype and c-myc Protein Levels in Primary Human Nonmalignant and Malignant Colonocytes, J Nutr 46,
96-105, 2001 |
Human
|
Human |
Epithelial and mucosal lymphocytes |
Neutral Protease: 0.1%
CLSPA: 0.02%
Deoxyribonuclease I: 0.01%
|
RPMI 1640 |
Hisamatsu, T., Watanabe, M., Ogata, H., Ezaki, T., Hozawa, S., Ishii, H., Kanai, T. and Hibi, T.: Interferon-Inducible Gene Family 1-8U Expression in Colitis-Associated Colon Cancer and Severely Inflamed Mucosa in Ulcerative Colitis., Cancer Res 59,
5927-31, 1999 |
Human
|
Human |
Colonic epithelial |
Neutral Protease: 1.2 u/ml
Collagenase Type 4: 50 u/ml
|
HBSS |
Gibson, P., Rosella, O., Wilson, A., Mariadason, J., Rickard, K., Byron, K. and Barkla, D.: Colonic Epithelial Cell Activation and the Paradoxical Effects of Butyrate., Carcinogenesis
20,
539, 1999 |
Human
|
Human |
Lamina propria lymphocytes |
Collagenase: 25 u/ml
|
HBSS |
Ueyama H, Kiyohara T, Sawada N, Isozaki K, Kitamura S, Kondo S, Miyagawa J, Kanayama S, Shinomura Y, Ishikawa H, Ohtani T, Nezu R, Nagata S, Matsuzawa Y: High Fas ligand expression on lymphocytes in lesions of ulcerative colitis, Gut 43,
48-55, 1998 |
Mouse
|
Mouse |
Lamina propria |
Collagenase: 100 u/ml
Deoxyribonuclease I: 0.004%
|
RPMI 1640 |
Larmonier, C., Shehab, K., Laubitz, D., Jamwal, D., Ghishan, F. and Kiela, P.: Transcriptional Reprogramming and Resistance to Colonic Mucosal Injury in Poly(ADP-ribose) Polymerase 1 (PARP1)-deficient Mice., J Biol Chem 291,
8918-30, 2016 |
Mouse
|
Mouse |
Colon organoid |
Collagenase Type 1: 800 u/ml
Neutral Protease: 0.013%
|
DMEM |
Fattahi, F., Steinbeck, J., Kriks, S., Tchieu, J., Zimmer, B., Kishinevsky, S., Zeltner, N., Mica, Y., El-Nachef, W., Zhao, H., de Stanchina, E., Gershon, M., Grikscheit, T., Chen, S. and Studer, L.: Deriving Human ENS Lineages for Cell Therapy and Drug Discovery in Hirschsprung Disease., Nature
531,
105-9, 2016 |
Mouse
|
Mouse, 8-10 week |
Lamina propria lymphocytes |
Neutral Protease: 3 u/ml
Collagenase Type 4: 0.05%
|
HBSS |
Round, J. and Mazmanian, S.: Inducible Foxp3+ Regulatory T-cell Development by a Commensal Bacterium of the Intestinal Microbiota., Proc Natl Acad Sci U S A 107,
12204-9, 2010 |
Mouse
|
Mouse |
Lamina propria |
Collagenase Type 2: 0.1%
Neutral Protease: 0.1%
Deoxyribonuclease I: 0.004%
|
RPMI 1640 |
Atarashi, K., Nishimura, J., Shima, T., Umesaki, Y., Yamamoto, M., Onoue, M., Yagita, H., Ishii, N., Evans, R., Honda, K. and Takeda, K.: ATP Drives Lamina Propria T(H)17 Cell Differentiation., Nature
455,
808, 2008 |
Mouse
|
Mouse, 6-8 week |
Cancer stem cell |
Collagenase Type 3: 200 u/ml
Deoxyribonuclease I: 100 u/ml
|
RPMI-1640 |
Dalerba Piero, Dylla Scott J, Park In-Kyung, Liu Rui, Wang Xinhao, Cho Robert W, Hoey Timothy, Gurney Austin, Huang Emina H, Simeone Diane M, Shelton Andrew A, Parmiani Giorgio, Castelli Chiara, Clarke Michael F: Phenotypic characterization of human colorectal cancer stem cells, Proc Natl Acad Sci U S A 104,
10158-63, 2007 |
Mouse
|
Mouse |
Lamina propria mononuclear cells |
Collagenase: 0.05%
Deoxyribonuclease I: 0.05%
Neutral Protease: 0.3%
|
HBSS |
Weigmann Benno, Tubbe Ingrid, Seidel Daniel, Nicolaev Alex, Becker Christoph, Neurath Markus F: Isolation and subsequent analysis of murine lamina propria mononuclear cells from colonic tissue, Nat Protoc
2,
2307-11, 2007 |
Mouse
|
Mouse |
Dentritic |
Collagenase: 300 u/ml
Deoxyribonuclease I: 0.002%
|
RPMI 1640 |
Abe, K., Nguyen, K., Fine, S., Mo, J., Shen, C., Shenouda, S., Corr, M., Jung, S., Lee, J., Eckmann, L. and Raz, E.: Conventional Dendritic Cells Regulate the Outcome of Colonic Inflammation Independently of T Cells., Proc Natl Acad Sci U S A 104,
17022, 2007 |
Mouse
|
Mouse |
Lympocytes |
Collagenase/Dispase: 100 u/ml
|
RPMI 1640 |
Annacker O, Coombes JL, Malmstrom V, Uhlig HH, Bourne T, Johansson-Lindbom B, Agace WW, Parker CM, Powrie F.: Essential role for CD103 in the T cell-mediated regulation of experimental colitis, J Exp Med 202,
1051, 2005 |
Mouse
|
Mouse, 6-8 week |
Lamina propria lymphocytes |
Collagenase Type 1: 0.2%
Deoxyribonuclease I: 0.01%
|
HBSS |
Totsuka T, Kanai T, Uraushihara K, Iiyama R, Yamazaki M, Akiba H, Yagita H, Okumura K, Watanabe M: Therapeutic effect of anti-OX40L and anti-TNF-alpha MAbs in a murine model of chronic colitis, Am J Physiol/Gastro 284,
G595-603, 2003 |
Mouse
|
Mouse, 6-8 week |
Lamina propria mononuclear cells |
Collagenase Type 2: 0.015%
Deoxyribonuclease I: 0.01%
|
RPMI |
Wirtz S., Becker C., Blumberg R., Galle P., and Neurath M.: Treatment of T cell-dependent experimental colitis in SCID mice by local administration of an adenovirus expressing IL-18 antisense mRNA, J Immunol 168(1),
411, 2002 |
Rat
|
Rat, adult |
Colon smooth muscle |
Soybean Trypsin Inhibitor: 0.01%
Collagenase Type 2: 0.1%
|
DMEM |
Somara, S., Bashllari, D., Gilmont, R. and Bitar, K.: Real-Time Dynamic Movement of Caveolin-1 During Smooth Muscle Contraction of Human Colon and Aged Rat Colon Transfected with Caveolin-1 cDNA., Am J Physiol Gastrointest Liver Physiol
300,
G1022, 2011 |
|
|