Tissue Dissociation References


	Tissue Dissociation References
	Tissue Type: Embryo

Embryo: Chicken
HH stage 23-24 chick embryos
	Wong, M., and Tuan, R.: Nuserum, A Synthetic Serum Replacement, Supports Chondrogenesis of Embryonic Chick Limb Bud Mesenchymal Cells in Micromass Culture, In Vitro Cell Dev Biol 29A, 917, 1993
Cells:	Mesenchymal cells
Enzyme(s)	Collagenase Type II: 1 mg/ml Trypsin: 1 mg/ml
Medium	DMEM(see reference)
Embryo: Chicken
19 day old White Leghorn chick embryos
	Gionti, E., Capasso, O., and Cancedda, R.: The Culture of Chick Embryo Chondrocytes and the Control of Their Differentiated Functions in Vitro, J Biol Chem 258 (11), 7190, 1983
Cells:	FibroblastsEpithelial-like cells
Enzyme(s)	Trypsin: 0.25% Collagenase Type II: 100 units/ml
Medium	E 199 medium
Embryo: Chicken
19 day old chick embryos
	Capasso, O., Gionti, E., Pontarelli, G., Ambesi-Impiobato, F., Nitsch, L., Tajana, G., and Cancedda, R.: The Culture of Chick Embryo Chondrocytes and the Control of Their Differentiated Functions In Vitro, Exp Cell Res 142, 197, 1982
Cells:	Chondrocytes
Enzyme(s)	Collagenase Type I: 7 U/ml Collagenase Type II: 100 U/ml Trypsin: 0.75 mg/ml
Medium	Coon's modified F-12
Embryo: Chicken
White Leghorn chick embryos
	Ahrens, P., Solursh, M., and Reiter, R.: Stage-Related Capacity for Limb Chondrogenesis in Cell Culture, Dev Biol 60, 69, 1977
Cells:	Wing buds
Enzyme(s)	Trypsin: 0.1% Collagenase: 0.1%
Medium	Saline G
Embryo: Mouse
17-19 day old Swiss mouse embryos
	Todaro, G., and Green, H.: Quantitative Studies of the Growth of Mouse Embryo Cells in Culture and Their Development into Established Lines, J Cell Biol 17, 299, 1963
Cells:	Mouse embryo cells
Enzyme(s)	Trypsin: 0.25%
Medium	DMEM