Endothelial - Tissue Tables
Species | Species Detail | Cells | Enzyme(s) | Medium |
Reference |
Bovine
|
Bovine |
Bovine umbilical cord (BUVEC) |
Collagenase: 0.1%
|
Dulbecco's/Ham F-12 |
Ricken Albert M, Traenkner Anja, Merkwitz Claudia, Hummitzsch Katja, Grosche Jens, Spanel-Borowski Katharina: The short prolactin receptor predominates in endothelial cells of micro- and macrovascular origin, J Vasc Res 44,
19-30, 2007 |
Bovine
|
Bovine |
Pulmonary artery endothelial and smooth muscle cells |
Collagenase: 0.04-0.05%
Soybean Trypsin Inhibitor: 0.04%
|
RPMI-1640 |
Yu, M., McAndrew, R., Al-Saghir, R., Maier, K., Medhora, M., Roman, R. and Jacobs, E.: Nitric Oxide Contributes to 20-HETE-Induced Relaxation of Pulmonary Arteries., J Appl Physiol 93,
1391, 2002 |
Bovine
|
Bovine, (Bos taurus), calf |
Endothelial Pulmonary |
Collagenase: 1000 u/ml
Collagenase Type 2: 1000 u/ml
|
PBS, CMF |
Del Vecchio, P., Siflinger-Birnboim, A., Belloni, P., Holleran, L., Lum, H., and Malik, A.: Culture and Characterization of Pulmonary Microvascular Endothelial Cells, In Vitro Cell Dev Biol 28A,
711, 1992 |
Bovine
|
Calf |
Endothelial |
Trypsin: 0.25%
|
HEPES |
Vender, R.: Role of Endothelial Cells in the Proliferative Response of Cultured Pulmonary Vascular Smooth Muscle Cells to Reduced Oxygen Tension, In Vitro Cell Dev Biol 28A,
403, 1992 |
Bovine
|
Bovine, adult |
Cerebral artery Endothelial |
Collagenase: 0.2%
|
HBSS |
Machi, T., Kassell, N.F., and Scheld, W.M.: Isolation and Characterization of Endothelial Cells From Bovine Cerebral Arteries, In Vitro Cell Dev Biol 26,
291, 1990 |
Bovine
|
Bovine |
Endothelial Aortic |
Trypsin: 0.05%
|
Krebs Ringer solution |
DeNucci, G., Gryglewski, R.J., Warner, T.D., and Vane, J.R.: Receptor-Mediated Release of Endothelium-Derived Relaxing Factor and Prostacyclin From Bovine Aortic Endothelial Cells Is Coupled, Proc Natl Acad Sci U S A 85,
2334, 1988 |
Bovine
|
Bovine |
Endothelial, pulmonary artery |
Collagenase: 0.1%
|
CMF-Dulbecco's PBS |
Martin, T.: Formation of Diacylglycerol by a Phospholipase D-phosphatidate Phosphatase Pathway Specific for Phosphatidylcholine in Endothelial Cells, Biochim Biophys Acta 962,
282, 1988 |
Bovine
|
Bovine |
Endothelial Aorta |
Collagenase Type 2: 0.1%
|
PBS |
Carson, M.P. and Haudenschild, C.C.: Microvascular Endothelium and Pericytes: High Yield, Low Passage Cultures, In Vitro Cell Dev Biol 22,
344, 1986 |
Bovine
|
Bovine |
Endothelial Aortic |
Collagenase Type 2: 0.1%
|
DMEM |
Kinsella, Michael G. and Wight, Thomas N.: Modulation of Sulfated Proteoglycan Synthesis by Bovine Aortic Endothelial Cells During Migration, J Cell Biol
102,
679, 1986 |
Bovine
|
Bovine |
Endothelial |
Collagenase: 0.5%
|
DMEM/Ham's F-12 |
Gospodarowicz, D., Massoglia, S., Cheng, J., and Fujii, D.: Effect of Fibroblast Growth Factor and Lipoproteins on the Proliferation of Endothelial Cells Derived From Bovine Adrenal Cortex, Brain Cortex, and Corpus Luteum Capillaries, J Cell Physiol 127,
121, 1986 |
Bovine
|
Bovine |
Endothelial Brain arteries |
Collagenase Type 2: 0.2%
|
Dulbecco's PBS |
Goetz, I.., Warren, J., Estrada, C., Roberts, E., and Krause, D.: Long-Term Cultivation of Arterial and Capillary Endothelium From Adult Bovine Brain, In Vitro Cell Dev Biol 21,
172, 1985 |
Bovine
|
Calf |
Endothelial Smooth muscle |
Collagenase: 0.75%
|
DMEM |
Voyta, J., Via, D., Butterfield, C., and Zetter, B.: Identification and Isolation of Endothelial Cells Based on Their Increased Uptake of Acetylated-Low Density Lipoprotein, J Cell Biol
99,
2034, 1984 |
Bovine
|
Bovine |
Endothelial Corneal |
Trypsin: 0.2%
|
PBS: DMEM |
Scott, D., Murray, J., and Barnes, M.: Investigation of the Attachment of Bovine Corneal Endothelial Cells, Exp Cell Res
144,
472, 1983 |
Bovine
|
Bovine, 2-3 weeks |
Endothelial Pulmonary artery |
Collagenase Type 1: 0.2%
|
RPMI 1640 w/1% Fetal Bovine Serum |
Lee, S., Douglas, W., Deneke, S., and Fanburg, B.: Ultrastructural Changes in Bovine Pulmonary Artery Endothelial Cells Exposed to 80% O2 In Vitro, In Vitro 19,
714, 1983 |
Bovine
|
Bovine |
Endothelial, Corneal |
Trypsin: 0.05%
|
0.01M Phosphate buffer with 0.02% EDTA 0.9% NaCl (See Reference) |
Robinson, J. and Gospodarowicz, D.: Glycosaminoglycans Synthesized by Cultured Bovine Corneal Endothelial Cells, J Cell Physiol 117,
368, 1983 |
Bovine
|
Bovine |
Endothelial Subclavian vein |
Collagenase Type 1: 0.10%
|
PBS |
Olander, J., Marasa, J., Kimes, R., Johnston, G., and Feder, J.: An assay measuring the stimulation of several types of bovine endothelial cells by growth factor(s) derived from cultured human tumor cells, In Vitro 18,
99, 1982 |
Bovine
|
Bovine |
Aortic Pulmonary artery |
Collagenase Type 2: 0.10%
|
PBS |
Makarski, J. S.: Stimulation of cyclic AMP production by vasoactive agents in cultured bovine aortic and pulmonary artery endothelial cells, In Vitro 17,
450, 1981 |
Bovine
|
Calf, fetal, 4-9 months |
Endothelial |
Collagenase Type 1: 0.25%
|
PBS |
Rosen, E., Mueller, S., Noveral, J., and Levine, E.: Proliferative Characteristics of Clonal Endothelial Cell Strains, J Cell Physiol 107,
123, 1981 |
Bovine
|
Bovine, adult |
Aorta |
Collagenase Type 1: 125 u/ml
|
Dulbecco's PBS with calcium and magnesium |
Cotta-Pereira, G., Sage, H., Bornstein, P., Ross, R., and Schwartz, S.: Studies of Morphologically Atypical ("Sprouting") Cultures of Bovine Aortic Endothelial Cells. Growth Characteristics and Connective Tissue Protein Synthesis, J Cell Physiol 102,
183, 1980 |
Bovine
|
Bovine, young |
Pulmonary artery |
Collagenase: 0.1%
|
Medium 199 |
Ryan, U., Mortara, M., and Whitaker, C.: Methods for Microcarrier Culture of Bovine Pulmonary Artery Endothelial Cells Avoiding the Use of Enzymes, Tissue Cell
12,
619, 1980 |
Bovine
|
Bovine |
Foreskin |
Collagenase: 0.5%
|
Dulbecco's MEM w/10% calf serum |
Folkman, J., Haudenschild, C. C., and Zetter, B. R.: Long-term Culture of Capillary Endothlial Cells, Proc Natl Acad Sci U S A 76,
5217, 1979 |
Bovine
|
Bovine |
Saphenous Vein Aorta |
Collagenase: 0.01%
|
PBS |
Eskin, S., Sybers, H., Trevino, L., Lie, J., and Chimoskey, J.: Comparison of Tissue-Cultured Bovine Endothelial Cells from Aorta and Saphenous Vein, In Vitro 14,
903, 1978 |
Bovine
|
Bovine |
Endothelial Thoracic aorta Saphenous veins |
Collagenase Type 2: 0.1%
|
PBS |
Schwartz, S.M.: Selection and Characterization of Bovine Aortic Endothelial Cells, In Vitro 14,
966, 1978 |
Bovine
|
Bovine |
Pulmonary artery |
Collagenase Type 2: 0.25%
|
Puck's solution |
Ryan, U.S., Clements, E., Habliston, D., and Ryan, J.W.: Isolation And Culture of Pulmonary Artery Endothelial Cells, Tissue Cell
10,
535, 1978 |
Bovine
|
Calf |
Endothelial |
Collagenase Type 2: 0.1%
|
DMEM |
Howard, B., Macarak, E., Gunson, D., and Kefalides, N.: Characterization of the Collagen Synthesized by Endothelial Cells in Culture, Proc Natl Acad Sci U S A 73 (7),
2361, 1976 |
Canine
|
Dog (also human) |
Microvessels |
Collagenase Type 4: 0.1%
|
DMEM |
Gerhart, D. Z., Broderius, M. A., and Drewes, L. R.: Cutlured Human and Canine Endothelial Cells from Brain Microvessels, Brain Res Bull 21,
785, 1988 |
Canine
|
Dog, mongrel, adult |
Endothelium Jugular vein |
Trypsin: 0.1%
|
Earle's PBS, CMF |
Ford, J., Burkel, W., and Kahn, R.: Isolation of Adult Canine Venous Endothelium for Tissue Culture, In Vitro 17,
44, 1981 |
Guinea-Pig
|
Guinea pig, female, 300-350g |
Coronary endothelial |
Collagenase Type 2: 0.1%
|
see reference |
Buxton I L, Kaiser R A, Oxhorn B C, Cheek D J: Evidence supporting the Nucleotide Axis Hypothesis: ATP release and metabolism by coronary endothelium, Am J Physiol Heart Circ Physiol
281,
H1657-66, 2001 |
Human
|
Human |
Vascular endothelial |
Collagenase Type 1: 0.1%
|
HBSS |
Ganguly, A., Zhang, H., Sharma, R., Parsons, S. and Patel, K.: Isolation of Human Umbilical Vein Endothelial Cells and Their Use in the Study of Neutrophil Transmigration Under Flow Conditions., J Vis Exp
66,
e4032, 2012 |
Human
|
Human, 18-68 yr |
Corneal endothelial |
Collagenase: 0.2%
Neutral Protease: 1.0%
|
DMEM/F12 |
Li Wei, Sabater Alfonso L, Chen Ying-Ting, Hayashida Yasutaka, Chen Szu-Yu, He Hua, Tseng Scheffer C G: A novel method of isolation, preservation, and expansion of human corneal endothelial cells, Inv Ophthal Visual Sci 48,
614-20, 2007 |
Human
|
Human |
Endothelial and vascular smooth muscle |
Collagenase Type 1: 0.2%
|
HBSS |
Moss, S., Bates, M., Parrino, P. and Woods, TC.: Isolation of Endothelial Cells and Vascular Smooth Muscle Cells from Internal Mammary Artery Tissue., Ochsner J
7,
133, 2007 |
Human
|
Human |
HUVEC |
Collagenase Type 1: 0.1%
|
HBSS |
Davis, J., Crampton, S. and Hughes. C.: Isolation of Human Umbilical Vein Endothelial Cells (HUVEC)., J Vis Exp
3,
183, 2007 |
Human
|
Human |
HUVEC |
Collagenase Type 4: 0.1%
|
RPMI 1640 |
Silva AP, Kaufmann JE, Vivancos C, Fakan S, Cavadas C, Shaw P, Brunner HR, Vischer U, and Grouzmann E.: Neuropeptide Y expression, localization and cellular transducing effects in HUVEC, Biol Cell 97(6),
457, 2005 |
Human
|
Human |
Endothelial |
Collagenase Type 2: 0.1%
|
DMEM |
Patel, V., Logan, A., Watkinson, J., Uz-Zaman, S., Sheppard, M., Ramsden, J. and Eggo, M.: Isolation and Characterization of Human Thyroid Endothelial Cells., Am J Physiol Endocrinol Metab
284,
E168, 2003 |
Human
|
Human |
Esophageal microvascular endothelial |
Collagenase Type 2: 0.2%
|
MCDB-131 |
Rafiee, P., Ogawa, H., Heidemann, J., Li, M., Aslam, M., Lamirand, T., Fisher, P., Graewin, S., Dwinell, M., Johnson, C., Shaker, R. and Binion, D.: Isolation and Characterization of Human Esophageal Microvascular Endothelial Cells: Mechanisms of Inflammatory Activation., Am J Physiol Gastrointest Liver Physiol
285,
G1277, 2003 |
Human
|
Human |
Colonic endothelial cells |
Collagenase Type 2: 0.25%
|
HBSS/5%FBS |
Wang D., Lehman R., Donner D., Matli M., Warren R., and Welton M.: Expression and Endocytosis of VEGF and Its Receptors in Human Colonic Vascular Endothelial Cells, Am J Physiol/Gastro 282,
G1088, 2002 |
Human
|
Human |
Pulmonary vascular endothelial cells |
Neutral Protease: 1.18 u/ml
Elastase: 10 u/ml
|
M199 |
Muller AM, Hermanns MI, Skrzynski C, Nesslinger M, Muller KM, and Kirkpatrick CJ.: Expression of the endothelial markers PECAM-1, vWf, and CD34 in vivo and in vitro, Exp Mol Pathol 72,
221, 2002 |
Human
|
Human |
HUVEC |
Collagenase Type 2: 0.1%
|
PBS |
Takano Manabu, Meneshian Avedis, Sheikh Emran, Yamakawa Yasuhiko, Wilkins Kirsten Bass, Hopkins Elise A, Bulkley Gregory B: Rapid upregulation of endothelial P-selectin expression via reactive oxygen species generation, Am J Physiol Heart Circ Physiol
283,
H2054-61, 2002 |
Human
|
Human |
Endothelial |
Trypsin: 2%
|
PBS |
Goolcharran, C., Cleland, J., Keck, R., Jones, A., and Borchardt, R.: Comparsion of the Rates of Deamidation, Diketopiperazine Formation and Oxidation in Recombinant Human Vascular Endothelial Growth Factor and Model Peptides, AAPS PharmSci 2 (1),
5, 2000 |
Human
|
Human |
HUVEC, porcine pulmonary arterial endothelial cells |
Collagenase Type 2: 0.2%
|
DMEM |
Kwak HJ, Lee SJ, Lee YH, Ryu CH, Koh KN, Choi HY, and Koh GY.: Angiopoietin-1 inhibits irradiation- and mannitol-induced apoptosis in endothelial cells, Circulation 101(19),
2317, 2000 |
Human
|
Human |
Vascular endothelial cells |
Collagenase: 0.1%
|
DMEM |
Schonbeck U, Sukhova GK, Graber P, Coulter S, Libby P: Augmented expression of cyclooxygenase-2 in human atherosclerotic lesions, Am J Pathol 155,
1281-91, 1999 |
Human
|
Human |
Hepatic endothelial cells |
Collagenase: 0.2%
|
DMEM |
Sanyal AJ, and Mirshahi F.: A simplified method for the isolation and culture of endothelial cells from pseudointima of transjugular intrahepatic portasystemic shunts, Lab Invest 78(11),
1469, 1998 |
Human
|
Human |
Foreskin microvascular endothelial |
Trypsin: 0.3%
|
HBSS |
Wojta J, Gallicchio M, Zoellner H, Filonzi E, Hamilton J, McGrath K: Interleukin-4 stimulates expression of urokinase-type-plasminogen activator in cultured human foreskin microvascular endothelial cells, Blood 81,
3285-92, 1993 |
Human
|
Human |
Endothelial |
Trypsin: 0.3.%
|
HBSS (see reference) |
Lee, K, Lawley, T, Xu, Y, and Swerlick, R.: VCAM-1-, ELAM-1-, and ICAM-1-Independent Adhesion of Melanoma Cells to Cultured Human Dermal Microvascular Endothelial Cells, J Invest Dermatol 98,
79, 1992 |
Human
|
Human |
Vascular endothelial |
Neutral Protease: 0.15%
Trypsin: 0.25%
|
M-199 |
Farber HW, Antonov AS, Romanov YA, Smirnov VN, Scarfo LM, Beer DJ: Cytokine secretion by human aortic endothelial cells is related to degree of atherosclerosis, Am J Physiol 262,
H1088-95, 1992 |
Human
|
Human |
Umbilical vein HUVEC |
Collagenase: 0.1%
|
Cord Buffer (See Reference) |
Grant, D.S., Lelkes, P.I., Fukuda, K., Kleinman, H.K.: Intracellular Mechanisms Involved in Basement Membrane Induced Blood Vessel Differentiation In Vitro, In Vitro Cell Dev Biol 27,
327, 1991 |
Human
|
Human |
Crypt cells |
Collagenase: 125 u/ml
|
RMPI 1640 |
Whitehead, R., and Eeden, P.: A Method For the Prolonged Culture of Colonic Epithelial Cells, J Tiss Cul Meth 13,
103, 1991 |
Human
|
Human |
Endothelial |
Collagenase Type 1: 0.2%
|
Medium 199 |
Fischer, E., Stingl, A., and Kirkpatrick, C.: Migration Assay for Endothelial Cells in Multiwells Application to Studies on the Effect of Opioids, J Immunol Methods 128,
235, 1990 |
Human
|
Human |
Human umbilical vein endothelial cells |
Collagenase Type 2: 75 u/ml
|
M199 |
Muller WA, Ratti CM, McDonnell SL, Cohn ZA: A human endothelial cell-restricted, externally disposed plasmalemmal protein enriched in intercellular junctions, J Exp Med 170,
399-414, 1989 |
Human
|
Human |
Microvessels |
Collagenase Type 4: 0.1%
|
DMEM |
Gerhart, D. Z., Broderius, M. A., and Drewes, L. R.: Cutlured Human and Canine Endothelial Cells from Brain Microvessels, Brain Res Bull 21,
785, 1988 |
Human
|
Human |
Endothelial/HUVEC Foreskin & umbilical cord |
Trypsin: 0.3%
|
HBSS/PBS, Medium 199 (see reference) |
Kubota, Y., Kleinman, H., Martin, G., and Lawley, T.: Role of Laminin and Basement Membrane in Morphological Differentiation of Human Endothelial Cells into Capillary-like Structures, J Cell Biol
107,
1589, 1988 |
Human
|
Human |
Umbilical cord Smooth muscle |
Collagenase: 0.1%
|
HEPES |
Hoshi, H., Kan, M., Chen, J., and McKeehan, W.: Comparative Endocrinology-Paracrinology-Autocrinology of Human Adult Large Vessel Endothelial and Smooth Muscle Cells, In Vitro Cell Dev Biol 24 (4),
309, 1988 |
Human
|
Human |
Endothelial Saphenous vein |
Collagenase Type 2: 0.1%
|
PBS, CMF |
Sharefkin, J.B., Fairchild, K.D., Albus, R.A., Cruess, D.F., and Rich, N.M.: The Cytoxic Effect of Surgical Glove Powder Particles on Adult Human Vascular Endothelial Cell Cultures: Implications for Clinical Uses of Tissue Culture Techniques, J Surg Res 41,
463, 1986 |
Human
|
Human |
Endothelial |
Collagenase: 0.1%
|
HEPES |
Hoshi, H., and McKeehan, W.: Isolation, Growth Requirements, Cloning, Prostacylclin Production and Life-Span of Human Adult Endothelial Cells in Low Serum Culture Medium, In Vitro Cell Dev Biol 22 (1),
51, 1986 |
Human
|
Human |
Endothelial Dermal |
Trypsin: 0.3%
|
PBS |
Marks, R.M., Czerniecki, M., and Penny, R.: Human Dermal Microvascular Endothelial Cells: An Improved Method for Tissue Culture and Description of Some Singular Properties in Culture, In Vitro Cell Dev Biol 21,
627, 1985 |
Human
|
Human |
Fibroblasts Foreskin |
Hyaluronidase: 0.10%
|
DMEM |
Gordon, P., Sussman, I., and Hatcher, V.: Long-Term Culture of Human Endothelial Cells, In Vitro 19,
661, 1983 |
Human
|
Human |
Iliac arteries |
Collagenase: 0.25%
|
PBS w/Ca++, Mg++, & BSA (see reference) |
Glassberg, M., Bern, M., Coughlin, S., Haudenschild, C., Hoyer, L., and Antoniades, H.: Cultured Endothelial Cells Derived from the Human Iliac Arteries, In Vitro 18,
859, 1982 |
Human
|
Human, adult |
Peripheral blood mononuclear Monocytes T cells Endothelial |
Collagenase: 0.25%
|
RPMI 1640 |
Ashida, E., Johnson, A., and Lipsky, P.: Human endothelial cell-lymphocyte interaction. Endothelial cells function as accessory cells necessary for mitogen-induced human T lymphocyte activation in vitro, J Clin Invest 67,
1490, 1981 |
Human
|
Human |
Microvascular endothelial Neonatal foreskins |
Neutral Protease: at 1000 u/ml
|
Konigsberg's modification of HBSS (See Reference) |
Sherer, G., Fitzharris, T., Faulk, W., and LeRoy, E.: Cultivation of Microvascular Endothelial Cells from Human Preputial Skin, In Vitro 16,
675, 1980 |
Human
|
Human (adult and child) |
Foreskin |
Collagenase: 0.5%
|
Dulbecco's MEM w/10% calf serum |
Folkman, J., Haudenschild, C. C., and Zetter, B. R.: Long-term Culture of Capillary Endothlial Cells, Proc Natl Acad Sci U S A 76,
5217, 1979 |
Human
|
Human |
Umbilical vein
|
Trypsin: 100 µg/ml
|
Tris-HCl,0.2 M |
Jaffe, E.A., Minick, C.R., Adelman, B., Becker, C.G., and Nachman, R.: Synthesis of Basement Membrane Collagen By Cultured Human Endothelial Cells, J Exp Med 144,
209, 1976 |
Human
|
Human |
Umbilical vein |
Collagenase Type 1: 125 u/ml
|
Dulbecco's PBS |
Gimbrone Jr., M.A.: Culture of Vascular Endothelium, Prog Hemost Thromb 3,
1, 1976 |
Human
|
Human |
Umbilical cord |
Collagenase: 0.1%
|
Dulbecco's PBS |
Gimbrone, M.A., Cotran, R.S., and Folkman, J.: Human Vascular Endothelial Cells in Culture: Growth and DNA Synthesis, J Cell Biol
60,
673, 1974 |
Human
|
Human |
Umbilical cord |
Collagenase: 0.2%
|
Cord buffer (See Reference) |
Jaffe, E., Nachman, R., Becker, C., and Minick, C.: Culture of Human Endothelial Cells Derived from Umbilical Veins. Identification by Morphologic and Immunologic Criteria, J Clin Invest 52,
2745, 1973 |
Human
|
Human |
Umbilical cord |
Trypsin NF 1:250: 0.125%
|
Saline, normal |
Lewis, L.J., Haok, J.C., Maca, R.D., and Fry, G.L.: Replication of Human Endothelial Cells in Culture, Science 181,
452, 1973 |
Human
|
Human |
Umbilical cord |
Trypsin NF 1:250: 0.25%
|
CMF solution |
Fryer, D.G., Birnbaum, G., and Luttrell, C.N.: Human Endothelium in Cell Culture, J Atheroscler Res 6,
151, 1966 |
Mouse
|
Mouse, 2 month |
Endothelial, CNS |
PDS kit: with modifications
Stemxyme 1: 0.1%
|
EC growth medium |
Sabbagh, M. and Nathans, J.: A Genome-wide View of the De-differentiation of Central Nervous System Endothelial Cells in Culture, Elife
,
1, 2020 |
Mouse
|
Mouse, 4 week |
Aortic endothelial |
Collagenase Type 1: 0.6%
Elastase: 0.25%
|
DMEM |
Esteban, S., Clemente, C., Koziol, A., Gonzalo, P., Rius, C., Martinez, F., Linares, P., Chaparro, M., Urzainqui, A., Andres, V., Seiki, M., Gisbert, J and Arroyo, A.: Endothelial MT1-MMP Targeting Limits Intussusceptive Angiogenesis and Colitis via TSP1/Nitric Oxide Axis., EMBO Mol Med
12,
e10862, 2020 |
Mouse
|
Mouse |
Coronary endothelial |
Collagenase Type 2: 0.1%
Neutral Protease: 0.6 u/ml
|
Kreb's |
Luo, S., Truong, A. and Makino, A.: Isolation of Mouse Coronary Endothelial Cells., J Vis Exp
,
, 2016 |
Mouse
|
Mouse |
Endothelial lung |
Collagenase Type 1: 0.2%
|
DMEM |
Wang, J., Sun, C., Gerdes, N., Liu, C., Liao, M., Liu, J., Shi, M., He, A., Zhou, Y., Sukhova, G., Chen, H., Cheng, X., Kuzuya, M., Murohara, T., Zhang, J., Cheng, X., Jiang, M., Shull, G. and Rogers, S.: Interleukin 18 Function in Atherosclerosis is Mediated by the Interleukin 18 Receptor and the Na-Cl Co-Transporter., Nat Med 21,
820-6, 2015 |
Mouse
|
Mouse |
Endothelial |
Collagenase Type 2: 0.1%
Neutral Protease: 0.25 u/ml
Deoxyribonuclease I: 0.0075%
|
DMEM |
Xiao, L., McCann, J. and Dudley, A.: Isolation and Culture Expansion of Tumor-specific Endothelial Cells., J Vis Exp
,
e53072, 2015 |
Mouse
|
Mouse |
Cerebral artery endothelial |
Neutral Protease: 4 u/ml
Elastase: 1 u/ml
Collagenase Type 1: 120 u/ml
|
DMEM |
Kochukov, M., Balasubramanian, A., Abramowitz, J., Birnbaumer, L. and Marrelli, S.: Activation of Endothelial Transient Receptor Potential C3 Channel is Required for Small Conductance Calcium-Activated Potassium Channel Activation and Sustained Endothelial Hyperpolarization and Vasodilation of Cerebral Artery., J Am Heart Assoc
3,
, 2014 |
Mouse
|
Mouse |
Tumor endothelial |
Collagenase Type 1: 0.2%
|
HBSS |
Kazerounian, S., Gerald, D., Huang, M., Chin, R, Udayakumar, D, Zheng, N., O'Donnell, R., Perruzzi, C., Mangiante, L., Pourat, J., Phung, T., Bravo-Nuevo, A., Shechter, S., McNamara, S. and Duhadaway, K.: RhoB Differentially Controls Akt Function in Tumor Cells and Stromal Endothelial Cells During Breast Tumorigenesis., Cancer Res 73,
50, 2013 |
Mouse
|
Mouse, 8-14 week |
Endothelial |
Collagenase Type 4: 0.2%
|
HBSS |
Imoukhuede, P. and Popel, A.: Expression of VEGF Receptors on Endothelial Cells in Mouse Skeletal Muscle., PLoS ONE
7,
e44791, 2012 |
Mouse
|
Mouse |
Endothelial |
Papain: 40 u/ml
Deoxyribonuclease I: 125 u/ml
|
DPBS |
Daneman, R., Zhou, L., Agalliu, D., Cahoy, J., Kaushal, A. and Barres, B.: The Mouse Blood-Brain Barrier Transcriptome: a New Resource for Understanding the Development and Function of Brain Endothelial Cells., PLoS ONE
5,
e13741, 2010 |
Mouse
|
Mouse, 7-10 week |
Liver endothelial |
Collagenase: 0.03%
|
DMEM |
Follenzi, A., Benten, D., Novikoff, P., Faulkner, L., Raut, S. and Gupta, S.: Transplanted Endothelial Cells Repopulate the Liver Endothelium and Correct the Phenotype of Hemophilia A Mice., J Clin Invest 118,
935, 2008 |
Mouse
|
Mouse, 4 week |
Endothelial kidney |
Collagenase Type 1: 0.1%
|
DMEM |
Kondo, S., Scheef, E., Sheibani, N. and Sorenson, C.: PECAM-1 Isoform-Specific Regulation of Kidney Endothelial Cell Migration and Capillary Morphogenesis., Am J Physiol Cell Physiol
292,
C2070, 2007 |
Mouse
|
Mouse, embryonic or yolk sac |
Endothelial |
Collagenase Type 3: 200 u/ml
Deoxyribonuclease I: 0.001%
|
PBS |
Braren Rickmer, Hu Huiqing, Kim YungHae, Beggs HilaryE, Reichardt LouisF, Wang Rong: Endothelial FAK is essential for vascular network stability, cell survival, and lamellipodial formation, J Cell Biol
172,
151-62, 2006 |
Mouse
|
Mouse, neonatal |
Microvascular endothelial |
Neutral Protease: 0.005%
Collagenase Type 1: 4%
|
DMEM |
Cha, S., Talavera, D., Demir, E., Nath, A. and Sierra-Honigmann, M.: A Method of Isolation and Culture of Microvascular Endothelial Cells from Mouse Skin., Microvasc Res 70,
198, 2005 |
Mouse
|
Mouse, 4 week |
Retinal endothelial cells |
Collagenase Type 1: 0.1%
|
DMEM |
Su X, Sorenson CM, and Sheibani N.: Isolation and characterization of murine retinal endothelial cells, Mol Vis
9,
171, 2003 |
Mouse
|
Mouse, male |
Endothelial cells from lymph node |
Collagenase Type 1: 0.1%
|
PBS |
Izawa D, Tanaka T, Saito K, Ogihara H, Usui T, Kawamoto S, Matsubara K, Okubo K, and Miyasaka M: Expression profile of active genes in mouse lymph node high endothelial cells, Int Immunol 11(12),
1989, 1999 |
Mouse
|
Mouse, 2-4 week |
Cerebrovascular Endothelial |
Collagenase/Dispase: 0.1%
|
PBS |
Sapatino, B., Welsh, C., Smith, C., Bebo, B., and Linticum, D.: Cloned Mouse Cerebrovascular Endothelial Cells That Maintain Their Differentiation Markers for Factor VIII, Low Density Lipoprotein, and Angiotensin-Converting Enzyme, In Vitro Cell Dev Biol 29A,
923, 1993 |
Porcine
|
Porcine, 6 month |
Valvular endothelial |
Neutral Protease: 2 u/ml
Collagenase Type 2: 60 u/ml
|
PBS |
Balaoing, L., Post, A., Lin, A., Tseng, H., Moake, J. and Grande-Allen, K.: Laminin Peptide-Immobilized Hydrogels Modulate Valve Endothelial Cell Hemostatic Regulation., PLoS ONE
10,
e0130749, 2015 |
Porcine
|
Porcine |
Valvular endothelial |
Collagenase Type 2: 300-600 u/ml
|
DMEM |
Gould, R. and Butcher, J.: Isolation of Valvular Endothelial Cells., J Vis Exp
46,
e2158, 2010 |
Porcine
|
Porcine (also bovine) |
Endothelial |
Collagenase Type 1:
|
DMEM w/ 10% calf serum |
Nugent, H., and Edelman, E.: Endothelial Implants Provide Long-Term Control of Vascular Repair in a Porcine Model of Arterial Injury, J Surg Res 99,
228, 2001 |
Porcine
|
Porcine |
HUVEC, porcine pulmonary arterial endothelial cells |
Collagenase Type 2: 0.2%
|
DMEM |
Kwak HJ, Lee SJ, Lee YH, Ryu CH, Koh KN, Choi HY, and Koh GY.: Angiopoietin-1 inhibits irradiation- and mannitol-induced apoptosis in endothelial cells, Circulation 101(19),
2317, 2000 |
Porcine
|
Porcine, 6-7 month |
Porcine pulmonary endothelial |
Collagenase Type 1: 0.3%
|
RPMI 1640 |
Hill-Kapturczak N, Kapturczak MH, Block ER, Patel JM, Malinski T, Madsen KM, and Tisher CC.: Angiotensin II-stimulated nitric oxide release from porcine pulmonary endothelium is mediated by angiotensin IV, J Am Soc Nephrol
10(3),
481, 1999 |
Porcine
|
Porcine |
Endothelial |
Trypsin: 0.25%
|
Medium 199 |
Shasby, S.: Endothelial Cells Grown On Permeable Membrane Supports, J Tiss Cul Meth 14,
247, 1992 |
Porcine
|
Porcine |
Endothelial |
Trypsin: 0.05%
|
DMEM |
Vischer, P., and Buddecke, E.: Alteration of Glycosytransferase Activities during Proliferation of Cultivated Arterial Endothelial Cells and Smooth Muscle Cells, Exp Cell Res
158,
15, 1985 |
Porcine
|
Porcine |
Endothelial Aortic |
Collagenase Type 2: 0.1%
|
Dulbecco-Vogt MEM w/o serum |
Dickinson, E. and Slakey, L.: Plasma-derived Serum as a Selective Agent to Obtain Endothelial Cultures from Swine Aorta, In Vitro 18,
63, 1982 |
Porcine
|
Porcine, 30-40 kg |
Endothelial Thoracic aorta |
Collagenase Type 4: 0.025%
Trypsin: 0.05%
|
Medium 199 and 0.01M EDTA |
Merrilees, M.J., and Scott, L.: Interaction of Aortic Endothelial and Smooth Muscle Cells in Culture, Atherosclerosis 39,
147, 1981 |
Porcine
|
Porcine, 20-30 week |
Endothelial Aortas Veins |
Collagenase: 0.1%
|
Medium 199 w/BSS and HEPES or NaHCO3 |
Slater, D.N., and Sloan, J.M.: The Porcine Endothelial Cell in Tissue Culture, Atherosclerosis 21,
259, 1975 |
Porcine
|
Porcine, 60-100 days |
Aorta |
Trypsin: 0.1%
|
Phosphate buffer (see reference) |
Coulson, W.F.: The Effect Of Proteolytic Enzymes on the Tensile Strength of Whole Aorta and Isolated Aortic Elastin, Biochim Biophys Acta 237,
378, 1971 |
Rabbit
|
Rabbit |
Corneal endothelial cells (CEC) |
Hyaluronidase: 0.05%
|
DMEM |
Choi, J., Ko, M., and Kay, E.: Subcellular Localization of the Expressed 18 kDa FGF-2 Isoform in Corneal Endothelial Cells, Mol Vis
6,
222, 2000 |
Rabbit
|
Rabbit, 2-3Kg |
Endothelial, aortic |
Elastase: 0.2%
|
Hanks solution |
Haley, N., Shio, H., Fowler, S.: Characterization of lipid-laden aortic cells from cholesterol-fed rabbits. I. Resolution of aortic cell populations by metrizamide density gradient centrifugation, Lab Invest 37,
287, 1977 |
Rat
|
Rat, SD, male, 250-300 g |
Smooth muscle, aorta |
Soybean Trypsin Inhibitor: 0.25%
|
HBSS with 0.2 mM Ca++ |
Schwertschlag, U.S., and Whorton, A.R.: Platelet-Activating Factor-Induced Homologous and Heterologous Desensitization In Cultured Vascular Smooth Muscle Cells, J Biol Chem 263,
13791, 1988 |
Rat
|
Rat, SD, male, 350 - 450 g |
Lipocytes Kupffer Sinusoidal endothelial |
Collagenase: 0.015%
|
DMEM/Ham's F-12 |
Friedman, S. and Roll, F.: Isolation and Culture of Hepatic Lipocytes,Kupffer Cells, and Sinusoidal Endothelial Cells by Density Gradient Centrifugation with Stractan, Anal Biochem
161,
207, 1987 |
Rat
|
Rat, Wistar, male, 3 mo |
Endothelial Kupffer Parenchymal |
Collagenase: 0.05%
Pronase: 0.25%
|
HBSS |
Nagelkenke, J., Barto, K., and VanBerkel, T.: In vivo and in vitro uptake and degradation of acetylated low density lipoprotein by rat liver endothelial, Kupffer, and parenchymal cells, J Biol Chem 258,
12221, 1983 |
Rat
|
Rat, Wistar-Kyoto, male, 100 - 200 g |
Endothelial Cerebral |
Collagenase Type 2: 0.05%
|
HBSS |
Diglio, C.A., Grammas, P., Filiberto Giacomelli, M.S., and Wiener, J.: Primary Culture of Rat Cerebral Microvascular Endothelial Cells, Lab Invest 46,
554, 1982 |
Rat
|
Rat, 300 G, and pig, 30-40 Kg |
Endothelial Thoracic aorta |
Collagenase Type 4: 0.025%
Trypsin: 0.05%
|
Medium 199 and 0.01M EDTA |
Merrilees, M.J., and Scott, L.: Interaction of Aortic Endothelial and Smooth Muscle Cells in Culture, Atherosclerosis 39,
147, 1981 |
Rat
|
Rat |
Foreskin |
Collagenase: 0.5%
|
Dulbecco's MEM w/10% calf serum |
Folkman, J., Haudenschild, C. C., and Zetter, B. R.: Long-term Culture of Capillary Endothlial Cells, Proc Natl Acad Sci U S A 76,
5217, 1979 |
Rat
|
Rat, Wistar, adult, 170 g |
Endothelial |
Trypsin: 0.5%
|
BSS |
Phillips, P., Kumar, P., Kumar, S., and Waghe, M.: Isolation And Characterization of Endothelial Cells From Rat And Cow Brain White Matter, J Anat 129,
261, 1979 |
|
|