Epithelial - Tissue Tables
Species | Species Detail | Cells | Enzyme(s) | Medium |
Reference |
Bovine
|
Bovine, fetal |
Epithelial Tracheal |
Neutral Protease: 2%
|
Dissociation medium, CMF |
Schumann, B.L., Cody, T.E., Miller, M.L., Leikauf, G.D.: Isolation, Characterization, and Long-Term Culture of Fetal Bovine Tracheal Epithelial Cells, In Vitro Cell Dev Biol 24,
211, 1988 |
Canine
|
Dog |
Tracheal |
Pronase: 0.1%
|
DMEM |
Virmani, A., Naziruddin, B., Desai, V., Lowry, J., Graves, D., and Sachdev, G.: Evidence for Secretion of High Molecular Weight Mucins by Canine Tracheal Epithelial Cells in Primary Culture: Effects of Select Secretagogues on Mucin Secretion, In Vitro Cell Dev Biol 28A,
120, 1992 |
Chicken
|
Chick, 5 day old |
Intestinal mesenchymal and epithelial |
Collagenase: 0.03%
|
DMEM |
Simon-Assmann, P and Kedinger, M: Embryonic Gut-Dissagregated Culture,Cell & Tissue Culture: Laboratory Procedures Vol. 1,Doyle, A., Griffiths, J., and Newell, D.,
John Wiley and Sons, Ltd.,
12A:3.1, 1995 |
Fish
|
Shark (Squalus acanthias) |
Rectal gland |
Collagenase: 0.2%
|
Ringer's solution |
Karnaky, Jr., K.J., Valentich, J.D., Currie, M.G., Oehlenschlager, W.F., and Kennedy, M.P.: Atriopeptin Stimulates Chloride Secretion in Cultured Shark Rectal Gland Cells, Am J Physiol 260,
1125, 1991 |
Fish
|
Shark (Squalus acanthias) |
Rectal gland |
Collagenase: 0.2%
|
Ringer's solution |
Valentich, J: Primary Cultures of Shark Rectal Gland Epithelial Cells: A Model for Hormone-Sensitive Chloride Transport, J Tiss Cul Meth 13,
149, 1991 |
Fish
|
Winter flounder, 200-500 g (Pseudopleuronectes americanus) |
Renal tubule |
Trypsin: 0.2%
Trypsin: 0.2%
|
CMF solution |
Dickman, K. and Renfro, J.: Primary Culture of Flounder Renal Tubule Cells:Transepithelial Transport, Am J Physiol 251,
424, 1986 |
Frog
|
Frog, Xenopus laevis, adult, female |
Colonic epithelial |
Collagenase Type 4: 0.1%
|
Kreb's |
Heinke, B, and Clauss, W.: Potassium Conductances in Isolated Single Cells from Xenopus Laevis Colonic Epithelium, J Comp Physiol [B] 169,
148, 1999 |
Guinea-Pig
|
Guinea-pig, Hartley, female, 200 g |
Epithelial |
Collagenase Type 1: 0.1%
|
DMEM |
Rutten, M.: Use of Commerically Available Cell Culture Inserts for Primary Culture and Electrophysiologic Studies of Guinea Pig Gastric Mucous Epithelial Cells, J Tiss Cul Meth 14,
235, 1992 |
Guinea-Pig
|
Guinea-pig, Hartley albino, 500-600 g |
Endometrial |
Collagenase: 0.25%
|
HBSS |
Chaminadas, G., Alkhalaf, M., Remy-Martin, J.P., Propper, A.Y., and Adessi, G.L.: Specific Effect of Oestrone Sulphate on Protein Synthesis and Secretion by Cultured Epithelial Cells from Guinea-pig Endometrium, J Endocrinol 123,
233, 1989 |
Hamster
|
Hamster, Syrian gold, male, 100-120 g |
Tracheal |
Pronase: 0.1%
|
MEM with Hepes, CMF |
Niles,R., Kim, K.C., Hyman, B., Christensen, T., Wasano, K., Brody, J.: Characterization Of Extended Primary And Secondary Cultures Of Hamster Tracheal Epithelial Cells, In Vitro Cell Dev Biol 24,
457, 1988 |
Hamster
|
Hamster, Syrian golden (strain CR:RGH) |
Tracheal |
Trypsin: 0.1%
|
Ham's F-12 |
McDowell, E., et al.: Differentiation of Tracheal Mucociliary Epithelium in Primary Cell Culture Recapitulates Normal Fetal Development and Regeneration Following Injury in Hamsters, Am J Pathol 129,
511, 1987 |
Hamster
|
Hamster (strain 1516 EHS and Lakeview), 8-12 weeks |
Tracheal |
Trypsin: 0.05%
|
Medium 199 |
Lee, T., Wu, R., Brody, A., Barrett, J., and Nettesheim, P.: Growth and Differentiation of Hamster Tracheal Epithelial Cells in Culture, Exp Lung Res 6,
27, 1984 |
Hamster
|
Hamster, Syrian gold, male, 6 weeks- 4 months |
Tracheal |
Trypsin: 0.25%
|
PBS with EDTA |
Goldman, W.E., Baseman, J.B.: Selective Isolation and Culture of a Proliferating Epithelial Cell Population from the Hamster Trachea, In Vitro 16,
313, 1980 |
Human
|
Human |
Epithelial |
Collagenase Type 4: 0.1%
Neutral Protease: 0.1%
|
PBS |
Gottipamula, S., Saraswat, S. and Sridhar, K.: Comparative Study of Isolation, Expansion and Characterization of Epithelial Cells., Cytotherapy
19,
263-271, 2017 |
Human
|
Human |
Oral mucosal epithelial |
Collagenase Type 1: 0.05%
|
DMEM/F12 |
Hsueh, Y., Huang, S., Lai, J., Ma, S., Chen, H., Wu, S., Wang, T., Sun, C., Ma, K., Chen, J., Lai, C. and Ma, D.: Preservation of Epithelial Progenitor Cells from Collagenase-Digested Oral Mucosa During Ex Vivo Cultivation., Sci Rep
6,
36266, 2016 |
Human
|
Human |
Gastrointestinal epithelial |
Collagenase Type 1: 0.2%
|
DMEM/F12 |
VanDussen, K., Marinshaw, J., Shaikh, N., Miyoshi, H., Moon, C., Tarr, P., Ciorba, M. and Stappenbeck, T.: Development of an Enhanced Human Gastrointestinal Epithelial Culture System to Facilitate Patient-Based Assays., Gut 64,
911-20, 2015 |
Human
|
Human |
Human tracheal epithelium |
Protease Type XIV: 0.04%
|
DMEM/F12 |
Widdicombe JH, Sachs LA, Morrow JL, and Finkbeiner WE.: Expansion of cultures of human tracheal epithelium with maintenance of differentiated structure and function, Biotechniques
39(2),
249, 2005 |
Human
|
Human |
Colonic epithelial |
Collagenase:
Neutral Protease: 0.3%
Deoxyribonuclease I: 0.05%
|
RPMI 1640 |
Fukushima, K. and Fiocchi, C.: Paradoxical Decrease of Mitochondrial DNA Deletions in Epithelial Cells of Active Ulcerative Colitis Patients., Am J Physiol Gastrointest Liver Physiol
286,
G804-13, 2004 |
Human
|
Human |
Corneal limbal epithelial sheet |
Neutral Protease: 5%
|
see reference |
Espana EM, Romano AC, Kawakita T, Di Pascuale M, Smiddy R, and Tseng SC: Novel enzymatic isolation of an entire viable human limbal epithelial sheet, Inv Ophthal Visual Sci 44(10),
4275, 2003 |
Human
|
Human |
Intestinal epithelial |
Collagenase Type 4: 72.5 u/ml
|
HBSS |
Fahlgren, A., Hammarstrom, S., Danielsson, A. and Hammarstrom, M.: Increased Expression of Antimicrobial Peptides and Lysozyme in Colonic Epithelial Cells of Patients with Ulcerative Colitis., Clin Exp Immunol 131,
90, 2003 |
Human
|
Human |
Gastric epithelial cells |
Collagenase Type 2: 200 u/ml
Neutral Protease: 1.2 u/ml
Soybean Trypsin Inhibitor: 0.125%
|
L-15 |
Smoot, D., Sewchand, J., Young, K., Desbrodes, B., Allen, C. and Naab, T.: A Method for Establishing Primary Cultures of Human Gastric Epithelial Cells, Meth Cell Sci 22,
133, 2000 |
Human
|
Human |
Gastric |
Collagenase Type 4: 0.01%
|
F-12 medium |
Sarosiek, J, Marshall, B, Peura, D, Guerrant, L, McCallum, R and Little, C: The Isolation and Maintenance of Human Gastric Epithelial Cells in Primary Culture,Cell & Tissue Culture: Laboratory Procedures Vol. 1,Doyle, A., Griffiths, J., and Newell, D.,
John Wiley and Sons, Ltd.,
12B:10.1, 1995 |
Human
|
Human |
Nasal polyp epithelial |
Neutral Protease: .004%
Trypsin: 0.1%
|
see reference |
Halbert CL, Alexander IE, Wolgamot GM, Miller AD: Adeno-associated virus vectors transduce primary cells much less efficiently than immortalized cells, J Virol 69,
1473-9, 1995 |
Human
|
Human |
Epithelial |
Trypsin: 0.05%
|
DMEM |
Sabatini, L., Allen-Hoffmann, B, Warner, T., and Azen, E.: Serial Cultivation of Epithelial Cells from Human and Macaque Salivary Glands, In Vitro Cell Dev Biol 27A,
939, 1991 |
Human
|
Human |
Epithelial |
Trypsin: 0.2%
|
MEM, PBS |
Robinson, C., and Wu, R.: Culture of Conducting Airway Epithelial Cells in Serum-Free Medium, J Tiss Cul Meth 13,
95, 1991 |
Human
|
Human |
Epithelial |
Collagenase: 2.0%
|
DMEM/Ham's F-12 |
Emerman, J. and Wilkinson, D.: Routine Culturing of Normal, Dysplastic and Malignant Human Mammary Epithelial Cells from Small Tissue Samples, In Vitro Cell Dev Biol 26,
1186, 1990 |
Human
|
Human |
Epithelial |
Pronase: 0.1%
|
PBS |
Gruenert, D.C., Basbaum, C.B., and Widdicombe, J.H.: Long-Term Culture of Normal and Cystic Fibrosis Epithelial Cells Grown Under Serum-Free Conditions, In Vitro Cell Dev Biol 26,
411, 1990 |
Human
|
Human |
Epithelial Sweat gland |
Collagenase Type 2: 0.2%
|
(see reference) |
Wood, L. and Neufeld, E.: A Cystic Fibrosis Phenotype in Cells Cultured from Sweat Gland Secretory Coil. Altered Kinetics of 36Cl Efflux, J Biol Chem 265,
12796, 1990 |
Human
|
Human (also bovine) |
Endometrial epithelial |
Trypsin:
|
DMEM/Ham's F-12 |
Munson, L., Chandler, S., and Schlafer, D.: Cultivation of Bovine Fetal and Adult Endometrial Epithelial Cells, J Tiss Cul Meth 11 (3),
129, 1988 |
Human
|
Human |
Epithelial |
Deoxyribonuclease I: 0.01%
|
HEPES with 5.9mM Glucose,5mM DTT |
Widdicombe, J.H., Coleman, D.L., Finkbeiner, W.E., and Tuet, I.K.: Electrical Properties of Monolayers Cultured From Cells of Human Tracheal Mucosa, J Appl Physiol 58,
1729, 1985 |
Human
|
Human |
Epithelial |
Protease Type XIV: 0.1%
|
Eagle's MEM |
Yankaskas, J., Cotton, C., Knowles, M., Gatzy, J., and Boucher, R.: Culture of Human Nasal Epithelial Cells on Collagen Matrix Supports, Am Rev Respir Dis 132,
1281, 1985 |
Human
|
Human, women, 27-49 years |
Epithelial Ovary |
Trypsin: 0.125%
|
HBSS, CMF |
Auersperg, N., Siemens, C.H., and Myrdal, S.E.: Human Ovarian Surface Epithelium In Primary Culture, In Vitro 20,
743, 1984 |
Human
|
Human, infant and neonate |
Epithelial Prostate |
Trypsin: 0.1%
|
HBSS |
Lechner, J., Babcock, M., Marnell, M., Narayan, K., and Kaighn, M.: Normal Human Prostate Epithelial Cell Cultures, Methods Cell Biol 21,
195, 1980 |
Mouse
|
Mouse, 8-12 week |
Nasal epithelium |
Papain: 20 u/ml
Deoxyribonuclease I: 0.005%
|
EBSS |
Brann, D., Tsukahara, T., Weinreb, C., Lipovsek, M., Van den Berge, K., Gong, B., Chance, R., Macaulay, I., Chou, H., Fletcher, R., Das, D., Street, K., de Bezieux, H., Choi, Y., Risso, D. and Dudoit, S.: Non-Neuronal Expression of SARS-CoV-2 Entry Genes in the Olfactory System Suggests Mechanisms Underlying COVID-19-Associated Anosmia., Sci. Adv.
6,
, 2020 |
Mouse
|
Mouse |
Alveolar epithelial |
Neutral Protease: 0.1%
Deoxyribonuclease I: 0.01%
|
DMEM |
Sun, F., Xiao, G. and Qu, Z.: Isolation of Murine Alveolar Type II Epithelial Cells., Bio-protocol
7,
, 2017 |
Mouse
|
Mouse |
Tracheal epithelial |
Papain: 50 u/ml
|
See reference |
Pardo-Saganta, A., Tata, P., Law, B., Saez, B., Chow, R., Prabhu, M., Gridley, T. and Rajagopal, J.: Parent Stem Cells Can Serve as Niches for Their Daughter Cells., Nature
523,
597-601, 2015 |
Mouse
|
Mouse, adult |
Cilary epithelial |
PDS kit: per instructions
|
EBSS |
Gualdoni, S., Baron, M., Lakowski, J., Decembrini, S., Pearson, R., Ali, R. and Sowden, J.: Isolation and Culture of Adult Ciliary Epithelial Cells, Previously Identified as Retinal Stem Cells, and Retinal Progenitor Cells., Curr Protoc Stem Cell Biol
Chapter 1,
Unit 1H.4, 2011 |
Mouse
|
Mouse, male, 8-16 week |
Renal tubular epithelial |
Collagenase: 200 u/ml
Soybean Trypsin Inhibitor: see reference
|
HBSS |
Breggia, A. and Himmelfarb, J.: Primary Mouse Renal Tubular Epithelial Cells have Variable Injury Tolerance to Ischemic and Chemical Mediators of Oxidative Stress., Oxid Med Cell Longev
1,
33, 2008 |
Mouse
|
Mouse |
Epithelial |
Collagenase: 0.025%
Neutral Protease: 0.25%
|
DMEM |
Mathew, R., Degenhard,t K., Haramaty, L., Karp, C. and White, E.: Immortalized Mouse Epithelial Cell Models to Study the Role of Apoptosis in Cancer., Methods Enzymol
446,
77-106, 2008 |
Mouse
|
Mouse, 6-8 week |
Lamina propria mononuclear cells |
Collagenase Type 2: 0.015%
Deoxyribonuclease I: 0.01%
|
RPMI |
Wirtz S., Becker C., Blumberg R., Galle P., and Neurath M.: Treatment of T cell-dependent experimental colitis in SCID mice by local administration of an adenovirus expressing IL-18 antisense mRNA, J Immunol 168(1),
411, 2002 |
Mouse
|
Mouse, 11 week |
Epithelial |
Collagenase Type 3: 25 u/ml
Hyaluronidase: 0.1%
Protease XIV: 0.05%
Deoxyribonuclease I: 0.04%
|
DMEM/F12 |
Mueller, S., Clark, J., Myers, P. and Korach, K.: Mammary Gland Development in Adult Mice Requires Epithelial and Stromal Estrogen Receptor Alpha., Endocrinology 143,
2357, 2002 |
Mouse
|
Mouse, female |
Salivary gland epithelial |
Collagenase Type 1: 750 u/ml
Hyaluronidase: 500 u/ml
|
DMEM/F12 |
Ishimaru N, Saegusa K, Yanagi K, Haneji N, Saito I, Hayashi Y: Estrogen deficiency accelerates autoimmune exocrinopathy in murine Sjogren's syndrome through fas-mediated apoptosis, Am J Pathol 155,
173-81, 1999 |
Mouse
|
Mouse, fetal 12-13 day |
Intestinal mesenchymal and epithelial |
Collagenase: 0.03%
|
DMEM |
Simon-Assmann, P and Kedinger, M: Embryonic Gut-Dissagregated Culture,Cell & Tissue Culture: Laboratory Procedures Vol. 1,Doyle, A., Griffiths, J., and Newell, D.,
John Wiley and Sons, Ltd.,
12A:3.1, 1995 |
Mouse
|
Mouse |
Submandibular salivary |
Collagenase Type 2 or 3: 0.16%
|
DMEM |
Durban, E: Submandibular Salivary Epithelial Cells,Cell & Tissue Culture: Laboratory Procedures Vol. 1,Doyle, A., Griffiths, J., and Newell, D.,
John Wiley and Sons, Ltd.,
12B:2.1, 1995 |
Mouse
|
Mouse |
Esophageal |
Trypsin: 0.25%
|
PBS, CMF |
Katayama, M., Kan, M.: Heparin-Binding (Fibroblast) Growth Factors re Potential Autocrine Regulators of Esophageal Epithelial Cell Proliferation, In Vitro Cell Dev Biol 27,
533, 1991 |
Mouse
|
Mouse, mature, female, 6-8-wk-old |
Uterine |
Trypsin: 0.2%
Trypsin: 0.2%
|
HBSS |
Ghosh, D., Danielson, K., Alston, J., and Heyner, S.: Functional Differentiation of Mouse Uterine Epithelial Cells Grown on Collagen Gels or Reconstituted Basement Membranes, In Vitro Cell Dev Biol 27A,
713, 1991 |
Mouse
|
Mouse, female, 18 - 20 days (also 20 - 22 days) |
Uterine |
Trypsin: 0.25%
|
HBSS |
Fukamachi, H., and McLachlan, J.: Proliferation and Differentiation of Mouse Uterine Epithelial Cells in Primary Serum-Free Culture: Estradiol-17B Suppresses Uterine Epithelial Proliferation Cultured on a Basement Membrane-Like Substratum, In Vitro Cell Dev Biol 27A,
907, 1991 |
Mouse
|
Mouse, (BALB/c), male, 3-5 months |
Epithelial Submandibular salivary gland |
Collagenase Type 3: 0.16% , 1:1 v/v
|
DMEM with 15 mM HEPES |
Durban, E.M.: Mouse Submandibular Salivary Epithelial Cell Growth and Differentiation in Long-Term Culture:Influence of the Extracellular Matrix, In Vitro Cell Dev Biol 26,
33, 1990 |
Mouse
|
Mouse, female |
Epithelial |
Pepsin: 0.1% and 0.05%
Pepsin: 0.1%
|
HBSS with 0.2% EDTA, CMF |
Riser, M., Huff, B., and Medina, D.: Pepsin Can Be Used To Subculture Viable Mammary Epithelial Cells, In Vitro 19,
730, 1983 |
Mouse
|
Mouse, (BALB/cfC3H or BALB/c) 8-12 day mid-pregnant |
Epithelial Submandibular gland |
Collagenase Type 3: 0.1%
|
HBSS |
Yang, J., Flynn, D., Larson, L., and Hamamoto, S.: Growth in Primary Culture of Mouse Submandibular Epithelial Cells, In Vitro 18,
435, 1982 |
Mouse
|
Mouse (BALB/cfC3H) |
Mammary tumors Epithelial |
Collagenase: 1.0%
|
HBSS |
Yang, J., Guzman, R., Richards, J., and Nandi, S.: Primary Cultures of Mouse Mammary Tumor Epithelial Cells Embedded in Collagen Gels, In Vitro 16,
502, 1980 |
Mouse
|
Mouse, C3H, 6-8 weeks |
Epithelial |
Collagenase: 0.10%
|
DMEM |
Lillehaug, J., Mondal, S., and Heidelberger, C.: Establishment of Epithelial Cell Lines from Mouse Regenerating Liver, In Vitro 15,
910, 1979 |
Porcine
|
Porcine, 5-60 kg |
Retinal pigment epithelial cells |
Collagenase: 2%
|
DMEM |
Wiencke, A., Kiilgaard, J., Nicolini, J., Bundgaard, M., Ropke, C., and La Cour, M.: Growth of Cultured Porcine Retinal Pigment Epithelial Cells, Acta Opthalmol Scand 81(2),
170, 2003 |
Porcine
|
Porcine |
Trachea |
Neutral Protease: 0.2%
|
HBSS |
De Buysscher, E., Kennedy, J., and Mendicino, J.: Synthesis of Mucin Glycoproteins by Epithelial Cells Isolated from Swine Trachea by Specific Proteolysis, In Vitro 20,
433, 1984 |
Rabbit
|
Rabbit, New Zealand white, adult |
Colon |
Neutral Protease: 0.3%
|
PBS |
Vidrich, A., Racindranath, R., Farsi, K., and Targan, S.: A Method for the Rapid Establishment of Normal Adult Mammalian Colonic Epithelial Cell Cultures, In Vitro Cell Dev Biol 24 (3),
188, 1988 |
Rabbit
|
Rabbit, New Zealand white, male, 4-5lb. |
Gastric Parietal and chief |
Collagenase Type 2: 0.08%
|
Sodium phosphate buffer |
Chew, C.S., Brown,M.R.: Release of Intracellular Ca2+ and Elevation of Inositol Triphosphate by Secretagogues in Parietal and Chief Cells Isolated from Rabbit Gastric Mucosa, Biochim Biophys Acta 888,
116, 1986 |
Rabbit
|
Rabbit, New Zealand white estrous, female, 4-5 months |
Mesothelial and surface epithelial Ovaries |
Trypsin: 0.125%-0.5%
|
Medium 199 |
Nicosia, S., Johnson, J., and Streibel, E.: Isolation and Ultrastructure of Rabbit Ovarian Mesothelium(Surface Epithelium), Int J Gynecol Pathol 3,
348, 1984 |
Rabbit
|
Rabbit, fetal |
Epithelial Gastric |
Collagenase Type 3: 0.10%
|
HBSS |
Logsdon, C.D., Bisbee, C.A., Rutten, M.J. and Machen, T.E.: Fetal Rabbit Gastric Epithelial Cells Cultured on Floating Collagen Gel, In Vitro 18,
233, 1981 |
Rat
|
Rat |
Mammary epithelial |
Collagenase Type 3: 0.35%
|
HBSS |
Mei, N., McDaniel, L., Dobrovolsky, V., Guo, X., Shaddock, J., Mittelstaedt, R., Azuma, M., Shelton, S., McGarrity, L., Doerge, D. and Heflich, R.: The Genotoxicity of Acrylamide and Glycidamide in Big Blue Rats., Toxicol Sci 115,
412, 2010 |
Rat
|
Rat, SD, adult, male, 8-10 weeks old |
Seminiferous tubules |
Trypsin: 0.05%
|
Krebs-Ringer bicarbonate buffer (see reference) |
Abou-Haila, A., and Tulsiani, D.: Acid Glycohydrolases in Rat Spermatocytes, Spermatids and Spermatozoa: Enzyme Activities, Biosynthesis and Immunolocalization, Biol Proced Online 3 (1),
35, 2001 |
Rat
|
Rat, male |
Alveolar epithelial |
Elastase: 40 u/ml
|
DMEM |
Planus, E., Galiacy, S., Matthay, M., Laurent, V., Gavrilovic, J., Murphy, G., Clerici, C., Isabey, D., Lafuma, C., and d'Ortho, M.: Role of Collagenase in Mediating in Vitro Alveolar Epithelial Wound Repair, J Cell Sci
112,
243, 1999 |
Rat
|
Rat, embryonic |
Tracheal epithelial |
Collagenase Type 4: 0.05%
Neutral Protease:
Deoxyribonuclease I:
|
DMEM/F12 |
Shannon JM, Gebb SA, and Nielsen LD: Induction of alveolar type II cell differentiation in embryonic tracheal epithelium in mesenchyme-free culture, Development
126,
1675, 1999 |
Rat
|
Rat, fetal, 14-15 day |
Intestinal mesenchymal and epithelial |
Collagenase: 0.03%
|
DMEM |
Simon-Assmann, P and Kedinger, M: Embryonic Gut-Dissagregated Culture,Cell & Tissue Culture: Laboratory Procedures Vol. 1,Doyle, A., Griffiths, J., and Newell, D.,
John Wiley and Sons, Ltd.,
12A:3.1, 1995 |
Rat
|
Rat, 4-14 week |
Retinal pigment epithelial cells |
Collagenase Type 1: 65 u/ml
Hyaluronidase: 220 u/ml
|
CF Hanks with EDTA |
Wang N, Koutz CA, and Anderson RE.: A method for the isolation of retinal pigment epithelial cells from adult rats, Inv Ophthal Visual Sci 34(1),
101, 1993 |
Rat
|
Rat, SD, adult, 90-120 day old, 350-450 g |
Epididymal epithelial |
Collagenase Type 2: 0.1%
|
HBSS |
Klinefelter, G.: A Novel System for the Co-Culture of Epididymal Epithelial Cells and Sperm From Adult Rats, J Tiss Cul Meth 14,
195, 1992 |
Rat
|
Rat, 6 day old |
Rat intestinal epithelial |
Neutral Protease: 0.01%
Collagenase: 300 u/ml
|
DMEM |
Evans GS, Flint N, Somers AS, Eyden B, and Potten CS.: The development of a method for the preparation of rat intestinal epithelial cell primary cultures, J Cell Sci
101,
219, 1992 |
Rat
|
Rat, SD, male, 150 - 250 g |
Epithelial Stomach |
Pronase: 0.15%
|
Medium 199 |
Dial, E., Kao, Y., and Lichtenberger, L.: Effects of 16,16-Dimethyl Prostaglandin E2 On Glycoprotein And Lipid Synthesis of Gastric Epithelial Cells Grown in a Primary Culture, In Vitro Cell Dev Biol 27,
39, 1991 |
Rat
|
Rat, adult (also hamster) |
Interlobular duct fragments |
Papain:
|
DMEM/Ham's F-12 |
Heimann, T., and Githens, S.: Rat Pancreatic Duct Epithelium Cultured on a Porous Support Coated with Extracellular Matrix, Pancreas 6 (5),
514, 1991 |
Rat
|
Rat, SD, 200 g |
Colon |
Deoxyribonuclease I: 10 µg/ml
|
(see reference) |
Yassin, R., Clearfield, H., Katz, S., and Murthy, S.: Gastrin Induction of mRNA Expression in Rat Colonic Epithelium In Vitro, Peptides
12,
63, 1991 |
Rat
|
Rat, Wistar, neonatal |
Epithelial |
Trypsin: 0.1%
|
HBSS |
Jassal, D., Han, R., Caniggia, I., Post, M., and Tanswell, A.: Growth of Distal Fetal Rat Lung Epithelial Cells in a Defined Serum-Free Medium, In Vitro Cell Dev Biol 27A,
625, 1991 |
Rat
|
Rodent, various (see reference) |
Epithelial |
Trypsin: 0.2%
|
MEM, PBS |
Robinson, C., and Wu, R.: Culture of Conducting Airway Epithelial Cells in Serum-Free Medium, J Tiss Cul Meth 13,
95, 1991 |
Rat
|
Rat, 6-8 day |
Retinal pigment epithelial |
Neutral Protease: 2%
|
DMEM |
Chang CW, Roque RS, Defoe DM, and Caldwell RB.: An improved method for isolation and culture of pigment epithelial cells from rat retina, Curr Eye Res 10(11),
1081, 1991 |
Rat
|
Rat (ACI/NMs X BUF/Mna) F1, male, 28 months Rat (ACI/MNs) male, 8 weeks |
Epthelial |
Collagenase Type 3: 0.1%
|
Eagle's MEM Serum-free |
Masuda, A., Ohtsuka, K., and Matsuyama, M.: Establishment of Functional Epithelial Cell Lines from a Rat Thyoma and a Rat Thymus, In Vitro Cell Dev Biol 26,
713, 1990 |
Rat
|
Rat, Fisher 344, male, 8 wks old, 250-300 g |
Tracheal epithelial |
Pronase: 0.5%
|
DMEM |
Chang, L., Wu, R., and Nettesheim, P.: Morphological Changes in Rat Tracheal Cells During The Adaptive and Early Growth Phase in Primary Cell Culture, J Cell Sci
74,
283, 1985 |
Rat
|
Rat, Fischer, male, 4-6 weeks |
Epithelial Esophagus |
Hyaluronidase: 0.1%
|
HEPES BSS |
Babcock, M., Marino, M., Gunning, W., and Stoner, G.: Clonal Growth and Serial Propagation of Rat Esophageal Epithelial Cells, In Vitro 19,
403, 1983 |
Rat
|
Rat, Fischer, Lewis and SD, male, 10-18 months |
Epithelial |
Trypsin: 0.05%
|
HBSS CMF |
Herring, A., Raychaudhuri, R., Kelley, S., and Iybe, P.: Repeated Establishment of Diploid Epithelial Cell Cultures from Normal and Partially Hepatectomized Rats, In Vitro 19,
576, 1983 |
Rat
|
Rat, SD, female |
Epithelial, cancer and tumor |
Collagenase: 0.1%
|
Eagles's MEM |
Cohen, L.: Isolation and Characterization of a Serially Cultivated, Neoplastic, Epithelial Cell Line from the N-nitrosomethylurea Induced Rat Mammary Adenocarcinoma, In Vitro 18,
565, 1982 |
Rat
|
Rat, Wistar, 12 day |
Epithelial |
Trypsin: 0.05%
|
HBSS, CMF |
Malan-Shibley, L., and Iype, P.: Influence of Cultures on Cell Morphology/Tyrosine Aminotransferase Levels, Exp Cell Res
131,
363, 1981 |
Rat
|
Rat, Fischer, adult, 200-250 g |
Epithelial |
Hyaluronidase: 0.0075%
|
KCl-NaCl HEPES Buffer |
Williams, G., and Gunn, J.: Long-Term Culture of Adult Rat Liver Epithelial Cells, Exp Cell Res
89,
139, 1974 |
Rat
|
Rat, Fischer, 10 day |
Epithelial-like |
Trypsin: 0.25%
|
PBS |
Williams, G., Weisburger, E., and Weisburger, J.: Isolation and Long-Term Cell Culture of Epithelial-Like Cells from Rat Liver, Exp Cell Res
69,
106, 1971 |
|
|