Eye - Tissue Tables
Species | Species Detail | Cells | Enzyme(s) | Medium |
Reference |
Bovine
|
Bovine |
Retinal endothelial |
Collagenase Type 4: 0.05%
Deoxyribonuclease I: 0.02%
Pronase: 0.02%
|
DMEM |
Terlizzi, V., Kolibabka, M., Burgess, J., Hammes, H. and Harmsen, M.: The Pericytic Phenotype of Adipose Tissue-Derived Stromal Cells Is Promoted by NOTCH2., Stem Cells 36,
240-251, 2018 |
Bovine
|
Bovine |
Pericyte |
Collagenase: 0.2%
|
DMEM |
Bryan, B. and D'Amore, P.: Pericyte Isolation and USe is Endothelial/Pericyte Coculture Models, Meth Enzymol 443,
315, 2008 |
Bovine
|
Bovine |
Microvascular endothelial |
Collagenase/Dispase: 0.1%
|
MEM |
Bowman, P., Betz, A., and Goldstein, G.: Primary Culture of Microvascular Endothelial Cells From Bovine Retina, In Vitro 18 (7),
626, 1982 |
Chicken
|
Chick, embryo, 6 day |
Retinal cells |
Trypsin: 0.005%
Deoxyribonuclease I: 0.005%
|
DMEM/F12 |
Jacob Vanessa, Rothermel Andrae, Wolf Peter, Layer Paul G: Rhodopsin, violet and blue opsin expressions in the chick are highly dependent on tissue and serum conditions, Cell Death Differ
180,
159-68, 2005 |
Chicken
|
Chick embryo |
Corneal epithelia |
Collagenase: 0.08%
Trypsin: 0.08%
|
HBSS |
Reenstra Wende R, Orlow Daniel L, Svoboda Kathy K H: ECM-stimulated signaling and actin reorganization in embryonic corneal epithelia are Rho dependent, Inv Ophthal Visual Sci 43,
3181-9, 2002 |
Chicken
|
Chick embryo (also rat) |
Retinal |
Trypsin: 0.6%
|
DMEM |
Seigel, G.: The Golden Age of Retinal Cell Culture, Mol Vis
5,
4, 1999 |
Chicken
|
Chick, embryo, 10-14 day |
Flat, retina |
Trypsin: 0.1%
|
Tyrode's solution, CMF |
Moyer, M., Bullrich, F., and Sheffield, J.: Emergence of Flat Cells From Glia in Stationary Cultures of Embryonic Chick Neural Retina, In Vitro Cell Dev Biol 26,
1073, 1990 |
Fish
|
Fish, zebrafish |
Retinal |
Papain: 10 u/ml
|
L-15 |
Diekmann, H., Kalbhen, P. and Fischer, D.: Characterization of Optic Nerve Regeneration using Transgenic Zebrafish., Front Cell Neurosci
9,
118, 2015 |
Fish
|
Goldfish |
Retinal Bipolar |
Papain: 30 u/ml
|
HEPES |
Graffe, M., Zenisek, D. and Taraska, J.: A Marginal Band of Microtubules Transports and Organizes Mitochondria in Retinal Bipolar Synaptic Terminals., J Gen Physiol 146,
109-17, 2015 |
Human
|
Human |
Corneal stromal keratocytes |
Collagenase Type 1: 0.1%
|
DMEM/F-12 |
Yusoff,B, Riau, A., Yam, G., Halim, N. and Mehta, J.: Isolation and Propagation of Human Corneal Stromal Keratocytes for Tissue Engineering and Cell Therapy.,Cells Vol. 11,,
,
, 2022 |
Human
|
Human |
Muller glia |
PDS kit: per instructions
|
DMEM |
Xu, N., Chen, Y., Dean, K., Lu, X., Liu, X., Wang, W., Dean, D., Kaplan, H., Gao, L., Dong, F. and Liu, Y.: Sphere-Induced Rejuvenation of Swine and Human Muller Glia Is Primarily Caused by Telomere Elongation., Stem Cells 35,
1579-1591, 2017 |
Human
|
Human |
Corneal stroma stem |
Collagenase Type 1: 0.1%
|
DMEM |
Sidney, L., Branch, M., Dua, H. and Hopkinson, A.: Effect of Culture Medium on Propagation and Phenotype of Corneal Stroma-Derived Stem Cells., Cytotherapy
17,
1706-22, 2015 |
Human
|
Human |
Corneal stromal stem |
Neutral Protease: 1.2 u/ml
Collagenase: 0.1%
|
DMEM |
Du, Y., Roh, D., Funderburgh, M., Mann, M., Marra, K., Rubin, J., Li, X. and Funderburgh, J.: Adipose-Derived Stem Cells Differentiate to Keratocytes In Vitro., Mol Vis
16,
2680, 2010 |
Human
|
Human, 18-68 yr |
Corneal endothelial |
Collagenase: 0.2%
Neutral Protease: 1.0%
|
DMEM/F12 |
Li Wei, Sabater Alfonso L, Chen Ying-Ting, Hayashida Yasutaka, Chen Szu-Yu, He Hua, Tseng Scheffer C G: A novel method of isolation, preservation, and expansion of human corneal endothelial cells, Inv Ophthal Visual Sci 48,
614-20, 2007 |
Human
|
Human |
Corneal limbal epithelial sheet |
Neutral Protease: 5%
|
see reference |
Espana EM, Romano AC, Kawakita T, Di Pascuale M, Smiddy R, and Tseng SC: Novel enzymatic isolation of an entire viable human limbal epithelial sheet, Inv Ophthal Visual Sci 44(10),
4275, 2003 |
Human
|
Human, 5-65 years |
Retinal pigment epithelial (RPE) |
Trypsin: 0.25%
|
HBSS |
Von Recum, H., Okano, T., Kim, S, and Bernstein, P.: Maintenance of Retinoid Metabolism in Human Retinal Pigment Epithelium Cell Culture, Exp Eye Res 69,
97, 1999 |
Monkey
|
Macaques and baboon |
Retinal |
Papain: 20-40 u/ml
Deoxyribonuclease I: 400 u/ml
|
Ames' solution |
Han ,Y., Jacoby, R. and Wu, S.: Morphological and Electrophysiological Properties of Dissociated Primate Retinal Cells., Brain Res 875,
175, 2000 |
Monkey
|
Monkey, cynomolgus, young adult |
Conjunctival lymphocytes |
Collagenase Type 1: 0.02%
|
RPMI 1640 |
Whittum-Hudson JA, Taylor HR: Antichlamydial specificity of conjunctival lymphocytes during experimental ocular infection, Infect Immun 57,
2977, 1989 |
Mouse
|
Mouse, 2-4 week |
Muller |
PDS kit: per instructions
|
DMEM |
Liu, X., Tang, L. and Liu Y.: Mouse Muller Cell Isolation and Culture., Bio-protocol
7,
, 2017 |
Mouse
|
Mouse |
Retinal |
Papain: 20 u/ml
Deoxyribonuclease I: 0.005%
|
PBS |
Ortin-Martinez, A., Tsai, E., Nickerson, P., Bergeret, M., Lu, Y., Smiley, S., Comanita, L. and Wallace, V.: A Reinterpretation of Cell Transplantation: GFP Transfer From Donor to Host Photoreceptors., Stem Cells 35,
932-939, 2017 |
Mouse
|
Mouse |
Retinal |
Papain: 20 u/ml
Deoxyribonuclease I: 0.005%
|
HBSS |
Wohl, S., Jorstad, N., Levine, E. and Reh, T.: Muller Glial microRNAs are Required for the Maintenance of Glial Homeostasis and Retinal Architecture., Nat Commun
8,
1603, 2017 |
Mouse
|
Mouse, C57BL/6 |
Retinal astrocytes |
Papain: 16.5 u/ml
Deoxyribonuclease I: 0.004%
|
HBSS |
Blandford, S. and Baldridge, W.: The Effect of Glutamate Receptor Agonists on Mouse Retinal Astrocyte [Ca(2+)]i., Biomed Res Int
2016,
8178162, 2016 |
Mouse
|
Mouse, adult |
Retinal photoreceptor |
PDS kit: with modifications
|
EBSS |
Feodorova, Y., Koch, M., Bultman, S., Michalakis, S. and Solovei, I.: Quick and Reliable Method for Retina Dissociation and Separation of Rod Photoreceptor Perikarya from Adult Mice., MethodsX
2,
39, 2015 |
Mouse
|
Mouse, 4-8 day |
Photoreceptors |
PDS kit: per instructions
|
Neurobasal |
Balmer, J., Zulliger, R., Roberti, S. and Enzmann, V.: Retinal Cell Death Caused by Sodium Iodate Involves Multiple Caspase-Dependent and Caspase-Independent Cell-Death Pathways., Int J Mol Sci 16,
15086-103, 2015 |
Mouse
|
Mouse, embryonic and P0 |
Retinal |
Papain: 8 u/ml
Deoxyribonuclease I: 124 u/ml
|
DPBS |
Dvoriantchikova, G., Perea-Martinez, I., Pappas, S., Barry, A., Danek, D., Dvoriantchikova, X., Pelaez, D. and Ivanov, D.: Molecular Characterization of Notch1 Positive Progenitor Cells in the Developing Retina., PLoS ONE
10,
e0131054, 2015 |
Mouse
|
Mouse |
Vitreous cells |
Collagenase: 0.1%
Hyaluronidase: 0.03%
|
DMEM |
Iqbal, N., Xu, L., Devitt, C. and Skapek, S.: Isolation and Characterization of Mammalian Cells Expressing the Arf Promoter During Eye Development., Biotechniques
56,
239-49, 2014 |
Mouse
|
Mouse, 6 week |
Retinal |
PDS kit: per instructions
|
DMEM |
Singh, M., Issa, P, Butler, R., Martin, C., Lipinski, D., Sekaran, S., Barnard, A. and Maclaren, R.: Reversal of End-Stage Retinal Degeneration and Restoration of Visual Bunction by Photoreceptor Transplantation., Proc Natl Acad Sci U S A ,
, 2013 |
Mouse
|
Mouse |
Retinal ganglion |
CLSPA: 240 u/ml
Hyaluronidase: 0.2%
|
Ames |
Schmidt, T. and Kofuji, P.: An Isolated Retinal Preparation to Record Light Response from Genetically Labeled Retinal Ganglion Cells., J Vis Exp
47,
2367, 2011 |
Mouse
|
Mouse, adult |
Cilary epithelial |
PDS kit: per instructions
|
EBSS |
Gualdoni, S., Baron, M., Lakowski, J., Decembrini, S., Pearson, R., Ali, R. and Sowden, J.: Isolation and Culture of Adult Ciliary Epithelial Cells, Previously Identified as Retinal Stem Cells, and Retinal Progenitor Cells., Curr Protoc Stem Cell Biol
Chapter 1,
Unit 1H.4, 2011 |
Mouse
|
Mouse, 4-5 week |
Lens Fiber |
Collagenase Type 4: 0.125%
Protease: 0.5%
|
PBS |
Ebihara, L., Tong, J., Vertel, B., White, T. and Chen, T.: Properties of Connexin 46 Hemichannels in Dissociated Lens Fiber Cells., Inv Ophthal Visual Sci 52,
882-9, 2011 |
Mouse
|
Mouse, postnatal day 1 |
Retinal progenitor |
Collagenase: 0.1%
|
HBSS |
Jiang, C. Klassen, H., Zhang, X. and Young, M.: Laser Injury Promotes Migration and Integration of Retinal Progenitor Cells into Host Retina., Mol Vis
16,
983, 2010 |
Mouse
|
Mouse, 4-8 week |
Corneal epithelial |
Neutral Protease: 1.5%
Trypsin: 0.25%
|
DMEM/F12 |
Kobayashi, T., Yoshioka, R., Shiraishi, A. and Ohashi, Y.: New Technique for Culturing Corneal Epithelial Cells of Normal Mice., Mol Vis
15,
1589-93, 2009 |
Mouse
|
Mouse |
Retinal |
Collagenase: 0.1%
Deoxyribonuclease I: 0.001%
|
RPMI |
Amadi-Obi, A., Yu, C., Liu, X., Mahdi, R., Clarke, G., Nussenblatt, R, Gery, I., Lee, Y. and Egwuagu, C.: TH17 Cells Contribute to Uveitis and Scleritis and are Expanded by IL-2 and Inhibited by IL-27/STAT1., Nat Med 13,
711, 2007 |
Mouse
|
mouse, adult |
Retinal |
Papain: 50 u/ml
|
DMEM/F-12 |
Jadhav AP, Cho SH and Cepko CL: Notch activity permits retinal cells to progress through multiple progenitor states and acquire a stem cell property, Proc Natl Acad Sci U S A 103,
18998, 2006 |
Mouse
|
Mouse, adult |
Stromal |
Trypsin: 0.05%
Collagenase: 78 u/ml
Hyaluronidase: 38 u/ml
|
DMEM/F-12 |
Yoshida, S., Shimmura, S., Nagoshi, N., Fukuda, K., Matsuzaki, Y., Okano, H. and Tsubota, K.: Isolation of Multipotent Neural Crest-Derived Stem Cells from the Adult Mouse Cornea., Stem Cells 24,
2714-22, 2006 |
Mouse
|
Mouse, 2-5 month |
Mouse retinal and bipolar |
Papain: 20 u/ml
Deoxyribonuclease I: 200 u/ml
|
HBSS |
Maxeiner Stephan, Dedek Karin, Janssen-Bienhold Ulrike, Ammermuller Josef, Brune Hendrik, Kirsch Taryn, Pieper Mario, Degen Joachim, Kruger Olaf, Willecke Klaus, Weiler Reto: Deletion of connexin45 in mouse retinal neurons disrupts the rod/cone signaling pathway between AII amacrine and ON cone bipolar cells and leads to impaired visual transmission, J Neurosci 25,
566-76, 2005 |
Mouse
|
Mouse, 4 week |
Retinal endothelial cells |
Collagenase Type 1: 0.1%
|
DMEM |
Su X, Sorenson CM, and Sheibani N.: Isolation and characterization of murine retinal endothelial cells, Mol Vis
9,
171, 2003 |
Porcine
|
Porcine, adult |
Muller glia |
PDS kit: per instructions
|
DMEM |
Xu, N., Chen, Y., Dean, K., Lu, X., Liu, X., Wang, W., Dean, D., Kaplan, H., Gao, L., Dong, F. and Liu, Y.: Sphere-Induced Rejuvenation of Swine and Human Muller Glia Is Primarily Caused by Telomere Elongation., Stem Cells 35,
1579-1591, 2017 |
Porcine
|
Porcine, 5-60 kg |
Retinal pigment epithelial cells |
Collagenase: 2%
|
DMEM |
Wiencke, A., Kiilgaard, J., Nicolini, J., Bundgaard, M., Ropke, C., and La Cour, M.: Growth of Cultured Porcine Retinal Pigment Epithelial Cells, Acta Opthalmol Scand 81(2),
170, 2003 |
Rabbit
|
Rabbit, New Zealand, Adult |
Corneal endothelial |
Collagenase Type 2: 0.2%
|
HBSS |
Lai, J., Cheng, H. and Ma, D.: Investigation of Overrun-Processed Porous Hyaluronic Acid Carriers in Corneal Endothelial Tissue Engineering, PLoS ONE
10,
1371, 2015 |
Rabbit
|
Rabbit |
Corneal keratocytes |
Hyaluronidase: 0.05%
Collagenase: 0.2%
|
DMEM |
Hao, M., Flynn, K., Nien-Shy, C., Jester, B., Winkler, M., Brown, D., La Schiazza, O., Bille, J., Jester, J.: In Vivo Non Linear Optical (NLO) Imaging in Live Rabbit Eyes Using the Heidelbert Two-Photon Laser Opthalmoscope, Exp Eye Res 91,
308, 2010 |
Rabbit
|
Rabbit, New Zealand |
Corneal keratocytes |
Trypsin: 0.25%
Collagenase: 0.5%
|
PBS |
Stramer Brian M, Kwok Michael G K, Farthing-Nayak Pamela J, Jung Jae-Chang, Fini M Elizabeth, Nayak Ramesh C: Monoclonal antibody (3G5)-defined ganglioside: cell surface marker of corneal keratocytes, Inv Ophthal Visual Sci 45,
807-12, 2004 |
Rabbit
|
Rabbit, adult |
Retinal neurons |
Papain: 26 u/ml
|
DMEM |
Brockway LM, Zhou Z-H, Bubien JK, Jovov B, Benos DJ, Keyser KT: Rabbit retinal neurons and glia express a variety of ENaC/DEG subunits, Am J Physiol Cell Physiol
283,
C126-34, 2002 |
Rabbit
|
Rabbit, New Zealand, adult, male, albino, 2 kg |
Epithelial |
Trypsin: 0.25%
|
HBSS/DMEM |
Johnson-Muller, B., and Gross, J.: Regulation of Corneal Collagenase Production: Epithelial-Stromal Cell Interactions, Proc Natl Acad Sci U S A 75 (9),
4417, 1978 |
Rat
|
Adult, female, 170-200 g |
Retinal |
PDS kit: with modifications
|
EBSS |
Mead, B. and Tomarev, S.: Bone Marrow-Derived Mesenchymal Stem Cells-Derived Exosomes Promote Survival of Retinal Ganglion Cells Through miRNA-Dependent Mechanisms., Stem Cells Transl Med 6,
1273-1285, 2017 |
Rat
|
Rat, Wister, 3-30 wk |
Retinal |
Deoxyribonuclease I: 0.005%
Papain: 30 u/ml
|
EBSS |
Cameron, M., Kekesi, O., Morley, J., Tapson, J., Breen, P., Van Schaik, A and Buskila, Y.: Calcium Imaging of AM Dyes Following Prolonged Incubation in Acute Neuronal Tissue., PLoS ONE
11,
e0155468, 2016 |
Rat
|
Rat, SD, 6-8 week |
Retinal |
PDS kit: per instructions
|
EBSS |
Vigneswara, V., Esmaeili, M., Deer, L., Berry, M., Logan, A. and Ahmed, Z.: Eye Drop Delivery of Pigment Epithelium-Derived Factor-34 Promotes Retinal Ganglion Cell Neuroprotection and Axon Regeneration., Mol Cell Neurosci 68,
212-21, 2015 |
Rat
|
Rat, SD |
Retinal ganglion |
PDS kit: with modifications
|
Neurobasal |
Tan, C., Andrews, M., Kwok, J., Heintz, T., Gumy, L., Fassler, R. and Fawcett, J.: Kindlin-1 Enhances Axon Growth on Inhibitory Chondroitin Sulfate Proteoglycans and Promotes Sensory Axon Regeneration., J Neurosci 32,
7325, 2012 |
Rat
|
Rat, SD, 180-220g |
Retinal ganglion |
Papain: 0.2%
|
Neurobasal |
Ma, J., Yu, W., Wang, Y., Cao, G., Cai, S., Chen, X., Yan, N., Yuan, Y., Zeng, H., Fleenor, D., Liu, X. and Pang, I.: Neuroprotective Effects of C-type Natriuretic Peptide on Rat Retinal Ganglion Cells., Inv Ophthal Visual Sci 51,
3544-53, 2010 |
Rat
|
Rat, Fisher, adult |
Retinal |
PDS kit: per instructions
|
MEM |
Suzuki, T., Mandai, M., Akimoto, M., Yoshimura, N. and Takahashi, M.: The Simultaneous Treatment of MMP-2 Stimulants in Retinal Transplantation Enhances Grafted Cell Migration into the Host Retina., Stem Cells 24,
2406, 2006 |
Rat
|
Rat, Dark Agouti, 3-4 week |
Ciliary-derived eye |
PDS kit: with modifications
|
DMEM/F12 |
Akagi T, Mandai M, Ooto S, Hirami Y, Osakada F, Kageyama R, Yoshimura N, Takahashi M.: Otx2 homeobox gene induces photoreceptor-specific phenotypes in cells derived from adult iris and ciliary tissue, Inv Ophthal Visual Sci 45,
4570, 2004 |
Rat
|
Rat, embryonic 17 day |
Retinal |
Trypsin: 0.6%
|
DMEM |
Seigel, G.: The Golden Age of Retinal Cell Culture, Mol Vis
5,
4, 1999 |
Rat
|
Rat, SD |
Retina |
Papain: 120 U
|
HBSS, PBS |
Jing, S., Wen, D., Yu, Y., Holst, P., Luo, Y., Fang, M., Tamir, R., Antonio, L., Hu, Z., et al.: GDNF-Induced Activation of the Ret Protein Tyrosine Kinase is Mediated by GDNFR-a, a Novel Receptor for GDNF, Cell
85,
1113, 1996 |
Rat
|
Rat, 4-14 week |
Retinal pigment epithelial cells |
Collagenase Type 1: 65 u/ml
Hyaluronidase: 220 u/ml
|
CF Hanks with EDTA |
Wang N, Koutz CA, and Anderson RE.: A method for the isolation of retinal pigment epithelial cells from adult rats, Inv Ophthal Visual Sci 34(1),
101, 1993 |
Rat
|
Rat, 6-8 day |
Retinal pigment epithelial |
Neutral Protease: 2%
|
DMEM |
Chang CW, Roque RS, Defoe DM, and Caldwell RB.: An improved method for isolation and culture of pigment epithelial cells from rat retina, Curr Eye Res 10(11),
1081, 1991 |
Rat
|
Rat, Long Evans, 1-15 days |
Neurons, visual cortex |
Papain: 20 u/ml
|
BSS (see reference) |
Huettner, J., and Baughman, R.: Primary Culture of Identified Neurons From the Visual Cortex of Postnatal Rats, J Neurosci 6,
3044, 1986 |
Rat
|
Rat, SD, pups |
Retina |
Trypsin: 0.25%
|
Ham's F-12 |
Sarthy PV, Curtis BM, and Catterall WA.: Retrograde Labeling, Enrichment, and Characerization of Retinal Ganglion Cells from the Neonatal Rat, J Neurosci 3 (12),
2532, 1983 |
Salamander
|
Salamander |
Neurons |
Papain: 14 u/ml
|
Ringers |
Clarke, R, Wang, J. and Townes-Anderson, E.: Using Laser Tweezers For Manipulating Isolated Neurons In Vitro., J Vis Exp
19,
911, 2008 |
Salamander
|
Salamander, 18-25 cm |
Retina |
Papain: 14 u/ml
|
Saline |
Townes-Anderson, E., MacLeish, P., and Raviola, E.: Rod Cells Dissociated from Mature Salamander Retina: Ultrastruture and Uptake of Horseradish Peroxidase, J Cell Biol
100,
175, 1985 |
Salamander
|
Salamander (A. tigrinum) |
Photoreceptors, retina |
Papain: 0.05%
|
(see reference) |
Bader, C., MacLeish, P., and Schwartz, E.: Responses to Light of Solitary Rod Photoreceptors Isolated From Tiger Salamander Retina, Proc Natl Acad Sci U S A 75,
3507, 1978 |
Turtle
|
Turtle (Pseudemys scripta elegans) |
Retinal |
Papain: 0.1% (13.5 u/mg)
|
Kreb's Ringer |
Lam, D.: Biosynthesis of Acetylcholine in Turtle Photoreceptors, Proc Natl Acad Sci U S A 69,
1987, 1972 |
|
|