Intestine - Tissue Tables
Species | Species Detail | Cells | Enzyme(s) | Medium |
Reference |
Canine
|
Dog, adult |
Intestinal L-cells |
Collagenase Type 1: 75 u/ml
|
HBSS |
Damholt AB, Buchan AM, Kofod H: Glucagon-like-peptide-1 secretion from canine L-cells is increased by glucose-dependent-insulinotropic peptide but unaffected by glucose, Endocrinology 139,
2085-91, 1998 |
Human
|
Human |
|
Collagenase Type 4: 0.3%
Deoxyribonuclease I: 5 ul/ml
|
|
Doyle, C., Vine, E, Bertram, K., Baharlou, H., Rhodes, J., Dervish, S., Gosselink, M., Di Re, A., Collins, G., Reza, F., Toh, J., Pathma-Nathan, N., Ahlenstiel, G., Ctercteko, G., Cunningham, A., Harm, A and Byrne, S.: Optimal Isolation Protocols for Examining and Interrogating Mononuclear Phagocytes From Human Intestinal Tissue.,Front Immunol Vol. 12,,
,
727952, 2021 |
Human
|
Human |
Lymphocytes |
CLSPA: 0.05%
Deoxyribonuclease I: 0.00075%
|
RPMI-1640 |
Trapecar, M., Khan, S., Roan, N., Chen, T., Telwatte, S., Deswal, M., Pao, M., Somsouk, M., Deeks, S., Hunt, P., Yukl, S. and Sanjabi, S.: An Optimized and Validated Method for Isolation and Characterization of Lymphocytes from HIV+ Human Gut Biopsies., AIDS Res Hum Retroviruses 33,
S31-S39, 2017 |
Human
|
Human |
Microvessel endothelial |
Collagenase Type 2: 0.2%
|
HBSS |
Kessler, S., Rho, H., West, G., Fiocchi, C., Drazba, J. and de la Motte, C.: Hyaluronan (HA) Deposition Precedes and Promotes Leukocyte Recruitment in Intestinal Inflammation., Clin Transl Sci 1,
57-61, 2008 |
Human
|
Human |
Lamina propria lymphocytes |
Collagenase: 20 u/ml
|
Medium |
Ebert, Ellen.: CD2 Activation of Human Lamina Propria Lymphocytes Reduces CD3 Responsiveness., Immunology 117,
71-7, 2006 |
Human
|
Human |
Lamina propria mononuclear cells |
Collagenase: 0.02%
|
HBSS |
Kanai Takanori, Totsuka Teruji, Uraushihara Koji, Makita Shin, Nakamura Tetsuya, Koganei Kazutaka, Fukushima Tsuneo, Akiba Hisaya, Yagita Hideo, Okumura Ko, Machida Utako, Iwai Hideyuki, Azuma Miyuki, Chen Lieping, Watanabe Mamoru: Blockade of B7-H1 suppresses the development of chronic intestinal inflammation, J Immunol 171,
4156-63, 2003 |
Human
|
Human |
Intestinal epithelial |
Collagenase Type 4: 72.5 u/ml
|
HBSS |
Fahlgren, A., Hammarstrom, S., Danielsson, A. and Hammarstrom, M.: Increased Expression of Antimicrobial Peptides and Lysozyme in Colonic Epithelial Cells of Patients with Ulcerative Colitis., Clin Exp Immunol 131,
90, 2003 |
Human
|
Human |
Mucosal mononuclear cells |
Collagenase Type 3: 0.01%
Deoxyribonuclease I: 0.01%
Soybean Trypsin Inhibitor: 0.01%
|
RPMI |
Stallmach A, Schaffer F, Hoffmann S, Weber S, Muller-Molaian I, Schneider T, Kohne G, Ecker KW, Feifel G, Zeitz M: Increased state of activation of CD4 positive T cells and elevated interferon gamma production in pouchitis, Gut 43,
499-505, 1998 |
Mouse
|
Mouse |
Lamina propria lymphocytes |
Collagenase Type 4: 0.02%
Deoxyribonuclease I: 0.01%
|
HBSS |
Emgard, J., Kammoun, H., Garcia-Cassani, B., Chesne, J., Parigi, S., Jacob, J., Cheng, H, Evren, E., Das, S., Czarnewski, P., Sleiers, N. Melo-Gonzalez, F., Kvedaraite, E. Svensson, M. and Scandella, E.: Oxysterol Sensing through the Receptor GPR183 Promotes the Lymphoid-Tissue-Inducing Function of Innate Lymphoid Cells and Colonic Inflammation., Immunity
48,
120-132.e8, 2018 |
Mouse
|
Mouse, embryonic |
Crypt |
Collagenase Type 1: 200 u/ml
Hyaluronidase: 100u/ml
|
DMEM |
Ren HJ, Zhang CL, Liu RD, Li N, Li XG, Xue HK, Guo Y, Wang ZQ, Cui J and Ming L: Primary Cultures of Mouse Small Intestinal Epithelial Cells using the Dissociating Enzyme Type I Collagenase and Hyaluronidase., Braz J Med Biol Res
50,
e5831, 2017 |
Mouse
|
Mouse |
Intestinal organoids |
Collagenase Type 4: 500 u/ml
Deoxyribonuclease I: 15 u/ml
|
Basal Medium |
O'Rourke, K., Ackerman, S., Dow, L. and Lowe, S.: Isolation, Culture, and Maintenance of Mouse Intestinal Stem Cells., Bio Protoc
6,
, 2016 |
Mouse
|
Mouse |
Lamina propria mononuclear cells |
Collagenase Type 3: 400 u/ml
Deoxyribonuclease I: 0.001%
|
HBSS |
Seo, S., Kuffa, P., Kitamoto, S., Nagao-Kitamoto, H., Rousseau, J., Kim, Y., Nunez, G. and Kamada, N.: Intestinal Macrophages Arising from CCR2(+) Monocytes Control Pathogen Infection by Activating Innate Lymphoid Cells., Nat Commun
6,
8010, 2015 |
Mouse
|
Mouse |
Lymphocytes |
Collagenase Type 1: 100 u/ml
|
RPMI-1640 |
Beura, L., Anderson, K., Schenkel, J., Locquiao, J., Fraser, K., Vezys, V., Pepper, M. and Masopust, D.: Lymphocytic Choriomeningitis Virus Persistence Promotes Effector-Like Memory Differentiation and Enhances Mucosal T cell Distribution., J Leukoc Biol 97,
217, 2015 |
Mouse
|
Mouse, P4 |
Enteric neural crest progenitors |
Neutral Protease: 0.5%
Collagenase animal free: 0.05%
|
DMEM/F12 |
Hotta, R., Stamp, L., Foong, J., McConnell, S., Bergner, A., Anderson, R., Enomoto, H., Newgreen, D., Obermayr, F., Furness, J. and Young, H.: Transplanted Progenitors Generate Functional Enteric Neurons in the Postnatal Colon., J Clin Invest 123,
1182, 2013 |
Mouse
|
Mouse |
Colon tumor organoids |
Collagenase Type 4: 200 u/ml
Neutral Protease: 0.01%
|
DMEM |
Xue, X. and Shah, Y.: In Vitro Organoid Culture of Primary Mouse Colon Tumors., J Vis Exp
,
e50210, 2013 |
Mouse
|
|
Enteric neurons |
Collagenase Type 4: 0.1%
Trypsin: 0.05%
Deoxyribonuclease I: 400 u/ml
|
DMEM/F12 |
Zhang, Y. and Hu, W.: Mouse Enteric Neuronal Cell Culture., Methods Mol Biol 1078,
55-63, 2013 |
Mouse
|
Mouse |
Intestinal organoids |
Collagenase Type 1: 800 u/ml
Neutral Protease: 0.013%
|
DMEM |
Barthel, E., Speer, A., Levin, D., Sala, F., Hou, X., Torashima, Y., Wigfall, C. and Grikscheit, T.: Tissue Engineering of the Intestine in a Murine Model., J Vis Exp
70,
e4279, 2012 |
Mouse
|
Mouse |
Dendritic, macrophages |
Collagenase: 0.15%
Deoxyribonuclease I: 0.004%
|
HBSS |
Geem, D., Medina-Contreras, O., Kim, W., Huang, C. and Denning T.: Isolation and Characterization of Dendritic Cells and Macrophages from the Mouse Intestine., J Vis Exp
63,
e4040, 2012 |
Mouse
|
Mouse |
Lyphocytes |
Collagenase Type 4: 0.05%
Neutral Protease: 0.1%
Deoxyribonuclease I: 200 ug/ml
|
RPMI 1640 |
Ruiz, V., Sachdev, M., Zhang, S., Wen, S. and Moss, S.: Isolating, Immunophenotyping and Ex vivo Stimulation of CD4+ and CD8+ Gastric Lymphocytes During Murine Helicobacter Pylori Infection., J Immunol Methods 384,
157-63, 2012 |
Mouse
|
Mouse, 2-3 month |
Enteric neural stem cells |
Collagenase: 0.11%
Neutral Protease: 0.1%
Deoxyribonuclease I: 50 u/ml
|
M199 |
Becker, L., Kulkarni, S., Tiwari, G., Micci, M. and Pasricha, P.: Divergent Fate and Origin of Neurosphere-Like Bodies from Different Layers of the Gut., Am J Physiol Gastrointest Liver Physiol
302,
G958-65, 2012 |
Mouse
|
Mouse, neonatal and adult |
Myenteric plexus |
Collagenase Type 4: 0.025-0.1%
Papain: 10 u/ml
Deoxyribonuclease I: 100 u/ml
|
HBSS |
Joseph, N., He, S., Quintana, E., Kim, Y., Nunez, G. and Morrison, S.: Enteric Glia are Multipotent in Culture but Primarily Form Glia in the Adult Rodent Gut., J Clin Invest 121,
3398, 2011 |
Mouse
|
Mouse |
Lamina propria lymphocytes |
Collagenase Type 1: 0.3%
Deoxyribonuclease I: 0.01%
|
RPMI 1640 |
Ito, Y., Kanai, T., Totsuka, T., Okamoto, R., Tsuchiya, K., Nemoto, Y., Yoshioka, A., Tomita, T., Nagaishi, T., Sakamoto, N., Sakanishi, T., Okumura, K., Yagita, H. and Watanabe, M.: Blockade of NKG2D Signaling Prevents the Development of Murine CD4+ T Cell-Mediated Colitis., Am J Physiol Gastrointest Liver Physiol
294,
G199, 2008 |
Mouse
|
Mouse |
Intestinal mononuclear |
Collagenase Type 4: 0.1%
|
RPMI 1640 |
Forbes, E., Groschwitz, K., Abonia, J.P., Brandt, E., Cohen, E., Blanchard, C., Ahrens, R., Seidu, L., McKenzie, A., Strait, R., Finkelman, F., Foster, P., Matthaei, K., Rothenberg, M. and Hogan, S.: IL-9- and Mast Cell-Mediated Intestinal Permeability Predisposes to Oral Antigen Hypersensitivity., J Exp Med 205,
897, 2008 |
Mouse
|
Mouse, Mastomys natalensis |
Enterochromaffin cells |
Collagenase: 0.025%
Pronase E: 0.07%
|
HBSS |
Kidd, M., Modlin, I., Eick, G. and Champaneria, M.: Isolation, Functional Characterization, and Transcriptome of Mastomys Ileal Enterochromaffin Cells., Am J Physiol Gastrointest Liver Physiol
291,
G778-91, 2006 |
Mouse
|
Mouse, 7 week |
Interstitial cells |
Collagenase: 0.04%
Trypsin: 0.02%
|
see reference |
Goto Kazunori, Matsuoka Satoshi, Noma Akinori: Two types of spontaneous depolarizations in the interstitial cells freshly prepared from the murine small intestine, J Physiol
559,
411-22, 2004 |
Mouse
|
Mouse, 9-13 day |
Interstitial cells of Cajal |
Collagenase Type 2: 0.13%
|
HBSS |
Ordag Tamas, Redelman Doug, Miller Lisa J, Horvath Viktor J, Zhong Qiao, Almeida-Porada Graca, Zanjani Esmail D, Horowitz Burton, Sanders Kenton M: Purification of interstitial cells of Cajal by fluorescence-activated cell sorting, Am J Physiol Cell Physiol
286,
C448-56, 2004 |
Mouse
|
Mouse, 0-15 day |
Interstitial cells of Cajal |
Collagenase Type 2: 0.13%
|
Hanks |
Lee Young Mee, Kim Byung Joo, Kim Hyun Jin, Yang Dong Ki, Zhu Mei Hong, Lee Kyu Pil, So Insuk, Kim Ki Whan: TRPC5 as a candidate for the nonselective cation channel activated by muscarinic stimulation in murine stomach, Am J Physiol/Gastro 284,
G604-16, 2003 |
Mouse
|
Mouse |
Lamina propria lymphocytes |
Collagenase Type 4: 300 u/ml
|
PBS |
Wu Y, Wang X, Csencsits KL, Haddad A, Walters N, Pascual DW: M cell-targeted DNA vaccination, Proc Natl Acad Sci U S A 98,
9318-23, 2001 |
Mouse
|
Mouse, F14 |
Intestinal mesenchymal |
Collagenase Type 2: 0.03%
|
HBSS |
Sakagami Y, Inaguma Y, Sakakura T, Nishizuka Y: Intestine-like remodeling of adult mouse glandular stomach by implanting of fetal intestinal mesenchyme, Cancer Res 44,
5845-9, 1984 |
Rat
|
Rat, postnatal, adult |
Gut neural crest stem |
Collagenase Type 4: 0.1%
Trypsin: 0.025%
|
HBSS |
Kruger, G., Mosher, J., Bixby, S., Joseph, N., Iwashita, T. and Morrison, S.: Neural Crest Stem Cells Persist in the Adult Gut but Undergo Changes in Self-Renewal, Neuronal Subtype Potential, and Factor Responsiveness., Neuron 35,
657, 2002 |
Rat
|
Rat, SD, embryonic |
Sciatic nerve and gut neural crest stem |
Collagenase Type 4: 0.025%
Trypsin: 0.005%
Deoxyribonuclease I: 0.05%
|
HBSS |
Bixby, S., Kruger, G., Mosher, J., Joseph, N. and Morrison, S.: Cell-Intrinsic Differences Between Stem Cells from Different Regions of the Peripheral Nervous System Regulate the Generation of Neural Diversity., Neuron 35,
643, 2002 |
Rat
|
Rat, male, 18-24 day |
Intestinal epithelial |
Collagenase Type 1: 0.1%
|
DMEM |
Quaroni A, Wands J, Trelstad RL, Isselbacher KJ: Epithelioid cell cultures from rat small intestine. Characterization by morphologic and immunologic criteria, J Cell Biol
80,
248-65, 1979 |
|
|