Kidney - Tissue Tables
Species | Species Detail | Cells | Enzyme(s) | Medium |
Reference |
Avian
|
Avian, house sparrows |
Tubule segments |
Collagenase Type 2: 0.1%
|
HBSS |
Goldstein D., Reddy V., and Plaga K.: Second Messenger Production in Avian Medullary Nephron Segments in Response to Peptide Hormones, Am J Physiol 276,
R847, 1999 |
Avian
|
Avian, chicken |
Tubule and glomeruli |
Collagenase Type 1: 0.1%
|
RPMI 1640 |
Reich, C. and Bonar, R.: Separation of Avian Kidney Tubules and Glomeruli for In Vitro Culture, Meth Cell Sci 7,
97, 1982 |
Bovine
|
Bovine |
Papillary duct |
Hyaluronidase: 0.2%
|
Keri's buffer HEPES buffered saline |
Husted, R., Hayashi, M., and Stokes, J.: Characteristics of Papillary Collecting Duct Cells in Primary Culture, Am J Physiol 255,
F1160, 1988 |
Canine
|
Canine |
Renal adipose derived cells |
Collagenase Type 1: 0.3%
|
DMEM |
Basu, J., Genheimer, C., Sangha, N., Quinlan, S., Guthrie, K., Kelley, R., Ilagan, R., Jain, D., Bertram, T. and Ludlow, J.: Organ Specific Regenerative Markers in Peri-Organ Adipose: Kidney., Lipids Health Dis
10,
171, 2011 |
Canine
|
Dog, 1-5 months |
Proximal tubules |
Collagenase Type 1: 0.15%
|
Krebs Ringer bicarbonate buffer |
States, B., Reynolds, R., Lee, J., and Segal, S.: Cystine Uptake By Cultured Cells Originating From Dog Proximal Tubule Segments, In Vitro Cell Dev Biol 26,
105, 1990 |
Canine
|
Dog, mongrel, 20 Kg |
Proximal tubular |
Deoxyribonuclease I: 0.0125%
|
(see reference) |
Yau, C., Rao, L., and Silverman, M.: Sugar Uptake Into a Primary Culture of Dog Kidney Proximal Tubular Cells, Can J Physiol Pharmacol 63,
417, 1985 |
Fish
|
Winter flounder (Pseudopleur-onectes americanus) 200 - 500 g |
Renal tubule |
Trypsin: 0.2%
Trypsin: 0.2%
|
CMF solution |
Dickman, K. and Renfro, J.: Primary Culture of Flounder Renal Tubule Cells:Transepithelial Transport, Am J Physiol 251,
424, 1986 |
Guinea-Pig
|
Guinea-pig, male, 100 g |
Single cells |
Trypsin: 0.25%
|
CF salt solution |
Phillips, H.: Dissociation of Single Cells from Lung or Kidney Tissue with Elastase, In Vitro 8,
101, 1972 |
Hamster
|
Hamster (also rat, SD and Wistar, 150-225 g, rabbit, bovine) |
Papillary duct |
Hyaluronidase: 0.2%
|
Keri's buffer HEPES buffered saline |
Husted, R., Hayashi, M., and Stokes, J.: Characteristics of Papillary Collecting Duct Cells in Primary Culture, Am J Physiol 255,
F1160, 1988 |
Human
|
Human |
Renal adipose derived cells |
Collagenase Type 1: 0.3%
|
DMEM |
Basu, J., Genheimer, C., Sangha, N., Quinlan, S., Guthrie, K., Kelley, R., Ilagan, R., Jain, D., Bertram, T. and Ludlow, J.: Organ Specific Regenerative Markers in Peri-Organ Adipose: Kidney., Lipids Health Dis
10,
171, 2011 |
Human
|
Human, adult |
Renal tumor cells and proximal tubular epithelial |
Collagenase Type 2: 0.1%
|
DMEM/F12 |
Valente, M., Henrique, R., Costa, V., Jeronimo, C., Carvalho, F., Bastos, M., Guedes de Pinho, P. and Carvalho, M.: A Rapid and Simple Procedure for the Establishment of Human Normal and Cancer Renal Primary Cell Cultures from Surgical Specimens., PLoS ONE
6,
e19337, 2011 |
Human
|
Human |
Renal cells |
Collagenase Type 4: 300 u/ml
Neutral Protease: see reference
|
HBSS |
Presnell, S., Bruce, A., Wallace, S., Choudhury, S., Genheimer, C., Cox, B., Guthrie, K., Werdin, E., Tatsumi-Ficht, P., Ilagan, R., Kelley, R., Rivera, E., Ludlow, J., Wagner, B., Jayo, M. and Bertram, T.: Isolation, Characterization, and Expansion Methods for Defined Primary Renal Cell Populations from Rodent, Canine, and Human Normal and Diseased Kidneys., Tiss Eng
17,
261, 2011 |
Human
|
Human, adult |
Renal proximal tubule and cortical fibroblasts |
Collagenase Type 2: 383 u/ml
|
DMEM/F-12 |
Johnson, D., Saunders, H., Johnson, F., Huq, S., Field, M., and Pollock, C.: Cyclosporin exerts a direct fibrogenic effect on human tubulointerstitial cells: roles of insulin-like growth factor I, transforming growth factor beta1, and platelet-derived growth factor, J Pharmacol Exp Ther 289,
535-42, 1999 |
Human
|
Human |
Renal cortex |
Trypsin: 0.1%
|
Tissue Culture Grade Water |
McAteer, J, Kempson, S., and Evan, A: Culture of Human Renal Cortex Epithelial Cells, J Tiss Cul Meth 13,
143, 1991 |
Human
|
Human |
Papillary duct |
Collagenase: 400 u/ml
|
Eagle's MEM-HEPES buffer w/L-glutamine |
Trifillis, A. and Kahng, M.: Characterization of an In Vitro System of Human Renal Papillary Collecting Duct Cells, In Vitro Cell Dev Biol 26,
441, 1990 |
Human
|
Human |
Mesangial |
Trypsin: 0.25%
|
DMEM/Ham's F-12 |
Heieren, M., van der Woude, F., and Balfour Jr., H.: Cytomegalovirus Replicates Efficiently in Human Kidney Mesangial Cells, Proc Natl Acad Sci U S A 85,
1642, 1988 |
Human
|
Human, adult |
Tubular |
Collagenase: 250 u/ml
|
PBS |
Yang, A., Gould-Kostka, J., and Oberley, T.: In Vitro Growth and Differentiation of Human Kidney Tubular Cells on a Basement Membrane Substrate, In Vitro Cell Dev Biol 23 (1),
34, 1987 |
Human
|
Human, adult, 14-66 years |
Tubular |
Collagenase: 100 u/ml
|
Joklik's MEM |
Trifillis, A., Regec, A., and Trump, B.: Isolation, Culture, and Charaterization of Human Renal Tubular Cells, J Urol 133,
324, 1985 |
Human
|
Human |
Malignant Stromal |
Papain: 0.009%
|
Sacks solution |
Hemstreet, G., Enoch, P., and Pretlow, T.: Tissue Disaggregation of Human Renal Cell Carcinoma with Further Isopyknic and Isokinetic Gradient Purification, Cancer Res 40,
1043, 1980 |
Human
|
Human, 24 newborn and stillborn (also rabbit) |
Renal |
Trypsin: 0.25%
|
See reference |
De Oca, H., and Malinin, T.: Dispersion and Cultivation of Renal Cells After Short-Term Storage of Kidneys, J Clin Microbiol 2,
243, 1975 |
Monkey
|
Monkey |
Kidney |
Trypsin: 0.01% - 0.00001%
|
Eagle's MEM |
Melnick, J., and Wallis, C.: Problems Related to the Use of Serum and Trypsin in the Growth of Monkey Kidney Cells, Dev Biol 37,
77, 1976 |
Mouse
|
Mouse |
Mactrophages |
Collagenase Type 4: 0.1%
Deoxyribonuclease I: 0.01%
|
DMEM |
Yuan, Y., Li, L., Zhu, L., Liu, F., Tang, X., Liao, G., Liu, J., Cheng, J., Chen, Y. and Lu, Y.: Mesenchymal Stem Cells Elicit Macrophages into M2 Phenotype Via Improving Transcription Factor EB-Mediated Autophagy to Alleviate Diabetic Nephropathy., Stem Cells 38,
639-652, 2020 |
Mouse
|
Mouse |
Kidney |
Deoxyribonuclease I: 125 u/ml
Protease, Bacillus Licheniformis: 1%
|
DPBS |
Adam, M., Potter, A. and Potter, S.: Psychrophilic Proteases Dramatically Reduce Single-Cell RNA-Seq Artifacts: A Molecular Atlas of Kidney Development., Development
144,
3625-3632, 2017 |
Mouse
|
Mouse |
Collecting duct epithelial |
Collagenase Type 2: 0.1%
|
DMEM |
DiRocco, D., Kobayashi, A., Taketo, M., McMahon, A. and Humphreys, B.: Wnt4/β-catenin Signaling in Medullary Kidney Myofibroblasts., J Am Soc Nephrol
24,
1399, 2013 |
Mouse
|
Mouse |
Glomeruli |
Collagenase Type 4: 0.1%
|
RPMI 1640 |
Kabgani, N., Grigoleit, T., Schulte, K., Sechi, A., Sauer-Lehnen, S., Tag, C., Boor, P., Kuppe, C., Warsow, G., Schordan, S., Mostertz, J., Chilukoti, R., Homuth, G., Endlich, N., Tacke, F. and Weiskirchen, R.: Primary Cultures of Glomerular Parietal Epithelial Cells or Podocytes with Proven Origin., PLoS ONE
7,
e34907, 2012 |
Mouse
|
Mouse |
Glomeruli |
Collagenase Type 1: 0.1%
Deoxyribonuclease I: 100 u/ml
|
DMEM |
Sedrakyan, S., Da Sacco, S., Milanesi, A., Shiri, L., Petrosyan, A., Varimezova, R., Warburton, D., Lemley, K., De Filippo, R. and Perin, L.: Injection of Amniotic Fluid Stem Cells Delays Progression of Renal Fibrosis., J Am Soc Nephrol
23,
661-73, 2012 |
Mouse
|
Mouse, 10-14 week |
Proximal tubule |
Collagenase Type 1: 0.1%
|
See reference |
Park, J., Pasupulati, R., Feldkamp, T., Roeser, N. and Weinberg, J.: Cyclophilin D and the Mitochondrial Permeability Transition in Kidney Proximal Tubules after Hypoxic and Ischemic Injury., Am J Physiol/Renal 301,
F134, 2011 |
Mouse
|
Mouse, embryonic |
Kidney |
Collagenase Type 1: 0.25%
Pancreatin: 1.0%
Deoxyribonuclease I: 1 u/ml
|
HBSS |
Brown, A., Blank, U., Adams, D., Karolak, M., Fetting, J., Hill, B. and Oxburgh, L.: Isolation and Culture of Cells from the Nephrogenic Zone of the Embryonic Mouse Kidney., J Vis Exp
50,
e2555, 2011 |
Mouse
|
Mouse, adult |
Kidney sphere-derived |
Collagenase Type 4: 0.1%
|
Iscove's Modified Dulbecco's |
Huang, Y., Johnston, P., Zhang, B., Zakari, A., Chowdhry, T., Smith, R., Marban, E., Rabb, H. and Womer, K.: Kidney-Derived Stromal Cells Modulate Dendritic and T Cell Responses., J Am Soc Nephrol
20,
831-41, 2009 |
Mouse
|
Mouse, male, 8-16 week |
Renal tubular epithelial |
Collagenase: 200 u/ml
Soybean Trypsin Inhibitor: see reference
|
HBSS |
Breggia, A. and Himmelfarb, J.: Primary Mouse Renal Tubular Epithelial Cells have Variable Injury Tolerance to Ischemic and Chemical Mediators of Oxidative Stress., Oxid Med Cell Longev
1,
33, 2008 |
Mouse
|
Mouse |
Proximal tubule |
Collagenase Type 2: 0.013%
|
see reference |
Wright, J., Morales, M., Sousa-Menzes, J., Ornellas, D., Sipes, J., Cui, Y., Cui, I., Hulamm, P., Cebotaru, V., Cebotaru, L., Guggino, W. and Guggino, S.: Transcriptional Adaptation to Clcn5 Knockout in Proximal Tubules of Mouse Kidney., Physiol Genomics
33,
341, 2008 |
Mouse
|
Mouse, 4 week |
Endothelial kidney |
Collagenase Type 1: 0.1%
|
DMEM |
Kondo, S., Scheef, E., Sheibani, N. and Sorenson, C.: PECAM-1 Isoform-Specific Regulation of Kidney Endothelial Cell Migration and Capillary Morphogenesis., Am J Physiol Cell Physiol
292,
C2070, 2007 |
Mouse
|
Mouse, adult |
Cortex, proximal tubule |
Collagenase: 0.15%
|
DMEM |
Syal Ashu, Schiavi Susan, Chakravarty Sumana, Dwarakanath Vangipuram, Quigley Raymond, Baum Michel: Fibroblast growth factor-23 increases mouse PGE2 production in vivo and in vitro, Am J Physiol/Renal 290,
F450-5, 2006 |
Mouse
|
Mouse |
Kidney mesenchymal |
Collagenase: 250 u/ml
Neutral Protease: 33.3 u/ml
|
DMEM/F12 |
Plotkin, M. and Goligorsky, M.: Mesenchymal Cells from Adult Kidney Support Angiogenesis and Differentiate into Multiple Interstitial Cell Types including Erythropoietin-Producing Fibroblasts., Am J Physiol Renal Physiol
291,
F902-12, 2006 |
Mouse
|
Mouse, 10-12 week |
Kidney |
Collagenase Type 4: 0.075%
|
DMEM |
Dekel, B., Zangi, L., Shezen, E., Reich-Zeliger, S., Eventov-Friedman, S., Katchman, H., Jacob-Hirsch, J., Amariglio, N., Rechavi, G., Margalit, R. and Reisner, Y.: Isolation and Characterization of Nontubular sca-1+lin- Multipotent Stem/Progenitor Cells from Adult Mouse Kidney., J Am Soc Nephrol
17,
3300, 2006 |
Mouse
|
Mouse, male, 12-16 week |
Renal proximal tubule cells |
Collagenase Type 2: 0.1%
Soybean Trypsin Inhibitor: 0.25%
|
DMEM/F-12 |
Cunningham, R., Xiaofei, E., Steplock, D., Shenolikar, S. and Weinman, E.: Defective PTH Regulation of Sodium-Dependent Phosphate Transport in NHERF-1-/- Renal Proximal Tubule Cells and Wild-Type Cells Adapted to Low-Phosphate Media., Am J Physiol Renal Physiol
289,
F933, 2005 |
Mouse
|
Mouse, male, 2 month |
Cortical collecting duct |
Collagenase: 54-178 u/ml
Protease: see reference
|
MEM |
Sindic, A., Velic, A., Basoglu, C., Hirsch, J., Edemir, B., Kuhn, M. and Schlatter, E.: Uroguanylin and Guanylin Regulate Transport of Mouse Cortical Collecting Duct Independent of Guanylate Cyclase C., Kidney Int
68,
1008, 2005 |
Mouse
|
Mouse, 6 week |
Glomerular mesangial cells |
Collagenase Type 4: 0.1%
|
RPMI 1640 |
Radeke HH, Janssen-Graalfs I, Sowa EN, Chouchakova N, Skokowa J, Loscher F, Schmidt RE, Heeringa P, Gessner JE.: Opposite regulation of type II and III receptors for immunoglobulin G in mouse glomerular mesangial cells and in the induction of anti-glomerular basement membrane (GBM) nephritis, J Biol Chem 277(30),
27535, 2002 |
Mouse
|
Mouse, 3-7 month |
Fibroblasts, mesangial, smooth muscle |
Trypsin: 0.25%
Collagenase: see reference
Soybean Trypsin Inhibitor: 0.05%
|
DMEM |
Bradshaw AD, Francki A, Motamed K, Howe C, Sage EH: Primary mesenchymal cells isolated from SPARC-null mice exhibit altered morphology and rates of proliferation, Mol Biol Cell
10,
1569-79, 1999 |
Mouse
|
Mouse |
Epithelial |
Collagenase Type 4: 1%
|
DMEM /F-12 |
Taub, M: Renal Tubule Cells,Cell & Tissue Culture: Laboratory Procedures Vol. 2,Doyle, A., Griffiths, J., and Newell, D.,
John Wiley & Sons, Ltd.,
14B:6.1, 1995 |
Mouse
|
Mouse, naive SJL (H-2S) |
Proximal tubular epithelial |
Deoxyribonuclease I: 15 µg/ml
|
RPMI 1640 |
Haverty, T., Kelly, C., Hines, W., Amenta, P., Watanabe, M., Harper, R., Kefalides, N., and Neilson, E.: Characterization of a Renal Tubular Epithelial Cell Line Which Secretes the Autologous Target Antigen of Autoimmune Experimental Interstitial Nephritis, J Cell Biol
107,
1359, 1988 |
Porcine
|
Porcine, hybrid |
Kidney |
Collagenase Type 1: 0.1%
|
HBSS |
Heussner, A. and Dietrich, D.: Primary Porcine Proximal Tubular Cells as an Alternative to Human Primary Renal Cells in Vitro: an Initial Characterization., BMC Cell Biol 14,
55, 2013 |
Rabbit
|
Rabbit |
Renal proximal tubular |
Collagenase Type 1: 60 u/ml
|
DMEM/F12 |
Nowak, G. and Bakajsova, D.: Assessment of Mitochondrial Functions and Cell Viability in Renal Cells Overexpressing Protein Kinase C Isozymes., J Vis Exp
71,
e4301, 2013 |
Rabbit
|
Rabbit, New Zealand |
Proximal tubule cells |
Collagenase Type 4: 0.005%
Soybean Trypsin Inhibitor: 0.00025%
|
DMEM/F-12 |
Taub, M.: Primary Kidney Proximal Tubule Cells., Methods Mol Biol 290,
231, 2005 |
Rabbit
|
Rabbit, New Zealand White, female |
Nephron segments |
Collagenase Type 2: 0.05%
Deoxyribonuclease I: 50 u/ml
|
Eagle's MEM |
Schafer, J., Watkins, M., Li, L., Herter, P., Haxelmans, S., and Schlatter, E.: A Simplified Method for Isolation of Large Numbers of Defined Nephron Segments, Am J Physiol 273,
F650, 1997 |
Rabbit
|
Rabbit, New Zealand white, male, 1.5 - 2.0 Kg |
Duct |
Soybean Trypsin Inhibitor: 0.025%
|
Hank's Solution with calcium and HEPES |
Naray-Fejes-Toth, A., and Fejes-Toth, G.: Immunoelection and Culture of Cortical Collecting Duct Cells, J Tiss Cul Meth 13,
179, 1991 |
Rabbit
|
Rabbit, New Zealand white, female, 2-3 Kg |
Renal proximal tubules |
Deoxyribonuclease I: 70 u/ml
|
Modified DME-F12 |
Rodeheaver, D., Aleo, M., and Schnellmann, R.: Differences in Enzymatic and Mechanical Isolated Rabbit Renal Proximal Tubules: Comparison in Long-Term Incubation, In Vitro Cell Dev Biol 26,
898, 1990 |
Rabbit
|
Rabbit (also rat, SD and Wistar, 150-225 g, hamster, bovine) |
Papillary duct |
Hyaluronidase: 0.2%
|
Keri's buffer HEPES buffered saline |
Husted, R., Hayashi, M., and Stokes, J.: Characteristics of Papillary Collecting Duct Cells in Primary Culture, Am J Physiol 255,
F1160, 1988 |
Rabbit
|
Rabbit, New Zealand, white, 2-3 kg |
MTALH cells RCCT cells |
Collagenase: 0.1%
|
DMEM |
Allen, M., Nakao, A., Sonnenburg, W., Burnatowska-Hledin, M., Speilman, W., and Smith, W.: Immunodissection of Cortical and Medullary Thick Ascending Limb Cells From Rabbit Kidney, Am J Physiol 255,
F704, 1988 |
Rabbit
|
Rabbit, New Zealand, white |
Collecting tubule |
Trypsin: 0.05%
|
Kreb's Ringer buffer |
Grenier, F., and Smith, W.: Formation of 6-keto-PGF-1aby Collecting Tubule Cells Isolated from Rabbit Renal Papillae, Prostaglandins 16,
759, 1978 |
Rabbit
|
Rabbit, New Zealand, white, female, 2.5 Kg |
Papillary collecting duct |
Collagenase: 0.2%
|
(see reference) |
Dworzack, D., and Grantham, J.: Preparation of Renal Papillary Collecting Duct Cells for Study, Nat Neurosci 8,
191, 1975 |
Rat
|
Rat, SD, fetal |
Fetal kidney |
Collagenase Type 4: 0.1%
|
MEM |
Gupta, A., Jadhav, S., Tripathy, N. and Nityanand, S.: Fetal Kidney Cells Can Ameliorate Ischemic Acute Renal Failure in Rats through Their Anti-Inflammatory, Anti-Apoptotic and Anti-Oxidative Effects., PLoS ONE
10,
e0131057, 2015 |
Rat
|
Rat, Lewis, male |
Renal adipose derived cells |
Collagenase Type 1: 0.3%
|
DMEM |
Basu, J., Genheimer, C., Sangha, N., Quinlan, S., Guthrie, K., Kelley, R., Ilagan, R., Jain, D., Bertram, T. and Ludlow, J.: Organ Specific Regenerative Markers in Peri-Organ Adipose: Kidney., Lipids Health Dis
10,
171, 2011 |
Rat
|
Rat |
Proximal tubule |
Collagenase Type 2: 0.1%
|
see reference |
Panico, C., Luo, Z., Damiano, S., Artigiano, F., Gill, P. and Welch, W.: Renal Proximal Tubular Reabsorption is Reduced in Adult Spontaneously Hypertensive Rats: Roles of Superoxide and Na+/H+ Exchanger 3., Hypertension 54,
1291, 2009 |
Rat
|
Rat, SD, male, 200-250g |
Medullary thick ascending limb |
Collagenase: 0.1%
|
HEPES-saline |
Silva, G., Beierwaltes, W., and Garvin, J.: Extracellular ATP stimulates NO production in rat thick ascending limb, Hypertension 47,
563-7, 2006 |
Rat
|
Rat, Wistar, 300g |
Renal proximal tubules |
Collagenase Type 2: 0.2%
|
DMEM/F12 |
Deng Aihua, Miracle Cynthia M, Lortie Mark, Satriano Joseph, Gabbai Francis B, Munger Karen A, Thomson Scott C, Blantz Roland C: Kidney oxygen consumption, carbonic anhydrase, and proton secretion, Am J Physiol/Renal 290,
F1009-15, 2006 |
Rat
|
Rat, Wistar, male, 300-350 g |
Renal proximal tubules |
Collagenase Type 2: 0.2%
|
DMEM/F-12 |
Deng, A., Miracle, C., Suarez, J., Lortie, M., Satriano, J., Thomson, S., Munger, K. and Blantz, R.: Oxygen Consumption in the Kidney: Effects of Nitric Oxide Synthase Isoforms and Angiotensin II., Kidney Int
68,
723, 2005 |
Rat
|
Rat |
Cortical tubule cells |
Collagenase: 0.6%
|
DMEM |
Arystarkhova, E., Wetzel R., and Sweadner K.: Distribution and Oligomeric Association of Splice Forms of Na(+)-K(+)-ATPase Regulatory Gamma-subunit in Rat Kidney, Am J Physiol/Renal 282(3),
F393, 2002 |
Rat
|
Rat, SD, male 275-300 g |
Glomerular mesangial cells |
Collagenase Type 1: 250 u/ml
|
DMEM |
Amiri Farhad, Shaw Sean, Wang Xiaodan, Tang Jie, Waller Jennifer L, Eaton Douglas C, Marrero Mario B: Angiotensin II activation of the JAK/STAT pathway in mesangial cells is altered by high glucose, Kidney Int Suppl 61,
1605-16, 2002 |
Rat
|
Rat, SD, 1 week |
Cortical collection duct |
Collagenase Type 2: 0.15%
|
DMEM/Ham's F12 |
Valencia L., Bidet M., Martial S., Sanchez E., Melendez E., Tauc M., Poujeol C., Martin D., Namorado M., Reyes J., and Poujeol P.: Nifedipine-activated Ca(2+) Permeability in Newborn Rat Cortical Collecting Duct Cells in Primary Culture, Am J Physiol Cell Physiol
280(5),
C1193, 2001 |
Rat
|
Rat, SD, 250-300 g |
Microvessels |
Collagenase Type 2: 0.1%
|
PSS |
Li, N., Teggatz, E., Li, P., Allaire, R., and Zou, A.: Formation and Actions of Cyclic ADP-Ribose in Renal Microvessels, Microvasc Res 60,
149, 2000 |
Rat
|
Rat, SD, 250-300 g |
Renal |
Collagenase Type 2: 0.2%
|
see reference |
Mattson D., and Wu F.: Nitric Oxide Synthase Activity and Isoforms in Rat Renal Vasculature, Hypertension 35,
337, 2000 |
Rat
|
Rat, SD, male, 5-6 week |
Renal tubules |
Collagenase: 0.1%
|
see reference |
Miyata, N., Park, F., Li, X., and Cowley, A.: Distribution of Angiotensin AT1 and AT2 Receptor Subtypes in the Rat Kidney, Am J Physiol 277,
F437, 1999 |
Rat
|
Rat, SD, 225-250 g |
Glomerular mesangial cells |
Collagenase Type 1: 250 u/ml
|
DMEM |
Amiri F, Garcia R: Regulation of angiotensin II receptors and PKC isoforms by glucose in rat mesangial cells, Am J Physiol 276,
F691-9, 1999 |
Rat
|
Rat, SD, male |
Proximal tubule suspensions |
Collagenase Type 4: 0.1%
Pronase E: 2 u/ml
|
HEPES buffer |
Eitle E, Hiranyachattada S, Wang H, Harris PJ: Inhibition of proximal tubular fluid absorption by nitric oxide and atrial natriuretic peptide in rat kidney, Am J Physiol 274,
C1075-80, 1998 |
Rat
|
Rat, SD, male |
Nephron segments |
Collagenase Type 2: 0.05%
Deoxyribonuclease I: 50 u/ml
|
Eagle's MEM |
Schafer, J., Watkins, M., Li, L., Herter, P., Haxelmans, S., and Schlatter, E.: A Simplified Method for Isolation of Large Numbers of Defined Nephron Segments, Am J Physiol 273,
F650, 1997 |
Rat
|
Rat, 12-weeks-old |
Renal |
Collagenase: 0.1%
|
DMEM |
Ishikawa, S., Kusaka, I., Higashiyama, M., Nagasaka, S., Saito, T., Honda, K., and Saito, T.: Cellular Signaling and Proliferative Action of AVP in Mesangium of SHR: Effect of Low Density Lipoprotein, Nat Neurosci 50,
1506, 1996 |
Rat
|
Rat, male, 150-200 g |
Epithelial Proximal tubule |
Protease: 0.1%
|
HBSS/CMF |
Elliget, K., and Trump, B.: Primary Cultures of Normal Rat Kidney Proximal Tubule Epithelial Cells for Studies of Renal Cell Injury, In Vitro Cell Dev Biol 27,
739, 1991 |
Rat
|
Rat, adult |
Inner medullary collecting duct Papillae |
Collagenase Type 2: 0.1%
|
PBS |
Brion, L., Schwartz, J., Lachman, H., Zavilowitz, B., and Schwartz, G.: Development of H+ Secretion by Cultured Renal Inner Medullary Collecting Duct Cells, Am J Physiol 257,
F486, 1989 |
Rat
|
Rat, Wistar, female, 100-150 g |
Glomerular mesangial |
Collagenase: 0.025%
|
RPMI 1640 |
Wang, J., Kester, M., and Dunn, M.: The Effects of Endotoxin on Platelet-Activating Factor Synthesis in Cultured Rat Glomerular Mesangila Cells, Biochim Biophys Acta 969,
217, 1988 |
Rat
|
Rat, Wistar, male, 150 - 200 g |
Renal target |
Collagenase: 1.0% (also 0.7%)
|
Eagle's MEM |
Barlet-Bas, C., Khadouri, C., Marsey, S., and Doucet, A.: Sodium-Independent In Vitro Induction of Na+,K+-ATPase by Aldosterone in Renal Target Cells: Permissive Effect of Triiodothyronine, Proc Natl Acad Sci U S A 85,
1707, 1988 |
Rat
|
Rat, Wistar, male, adult |
Proximal tubules |
Collagenase: 0.2%
|
Krebs-Henseleit buffer |
Gesek, F., Wolff, D., and Strandhov, J.: Improved Separation Method for Rat Proximal and Distal Renal Tubules, Am J Physiol 253,
F358, 1987 |
Rat
|
Rat,Wistar, 300-400 gm |
Proximal tubules |
Collagenase: 0.15 %
|
Krebs Henseleit solution |
Vinay, P., Gougoux, A., and Lemieux, G.: Isolation of a Pure Suspension of Rat Proximal Tubules, Am J Physiol 241,
F403, 1981 |
Rat
|
Rat |
Fibroblasts Kidney |
Trypsin: up to 0.25%
|
Dulbecco-Vogt MEM |
Wallach, D., Anderson, W., and Pastan, I.: Activation of Adenylate Cyclase in Cultured Fibroblasts by Trypsin, J Biol Chem 253,
24, 1978 |
|
|