Liver - Tissue Tables

SpeciesSpecies DetailCellsEnzyme(s)Medium Reference
Avian Avian, various Hepatocytes Collagenase Type 1: 0.1%
 see reference Else, P., Brand, M., Turner, N. and Hulbert, A: Respiration Rate of Hepatocytes Varies With Body Mass in Birds, J Exp Biol 207, 2305, 2004
Avian Ducklings, 7 day Hepatocytes Collagenase: 0.05%
Hyaluronidase: 0.05%
 DMEM/ F12 Lee J., Culvenor J., Angus P., Smallwood R., Nicoll A., and Locarnini S.: Duck Hepatitis B Virus Replication in Primary Bile Duct Epithelial Cells, J Virol 75(16), 7651, 2001
Canine Dog, 13 years old
(also rat, guinea-pig, rabbit, human)		 Hepatocytes Collagenase: 90 u/ml
 CF EGTA perfusate Reese, J. and Byard, J.: Isolation And Culture of Adult Hepatocytes from Liver Biopsies, In Vitro 17, 935, 1981
Canine Dog, adult Hepatocytes Trypsin: 0.1%
 HBSS, CMF Vickrey, H., Ramon, J., and McCann, D.: Continuous Culture of Normal Adult Mammalian Hepatocytes Exhibiting Parenchymal Functions, In Vitro 15, 120, 1979
Chicken Chicken, adult Hepatocytes Collagenase: 0.02%
 HEPES, CF Fraslin, J., Touquette, L., Douaire, M., Menezo, Y., Guillemot, J., and Mallard, J.: Isolation and Long Term Maintenance of Differentiated Adult Chicken Hepatocytes in Primary Culture, In Vitro Cell Dev Biol 28, 615, 1992
Chicken Chicken
(also rat, SD, 150-250 g)		 Hepatocytes Collagenase Type 4: 6000 units
Collagenase Type 3 & 4:
HEPES Weigel, P., Schnaar, R., Kuhlenschmidt, M., Schmell, E., Lee, R., Lee, Y., and Roseman, S.: Adhesion of Hepatocytes to Immobilized Sugars, J Biol Chem 254 (21), 10830, 1979
Chicken Chicken, Leghorn, white, 10-15 day Hepatocytes Deoxyribonuclease I: 0.00125%
 PBS Tarlow, D., Watkins, P., Reed, R., Miller, R., Zwergel, E., and Lane, M.: Lipogenesis and the Synthesis and Secretion of Very Low Density Lipoprotein by Avian Liver Cells in Nonproliferating Monolayer Culture, J Cell Biol 73, 332, 1974
Chicken Chick embryos, 5 day Heart
Liver		 Trypsin: 3.0%
 Tyrode's solution, CMF Steinberg, M.: "ECM": Its Nature, Origin, And Function in Cell Aggregation, Exp Cell Res 30, 257, 1963
Chicken Chick embryonic Various tissues (heart, liver, skeletal, cardiac) Trypsin: various grades
 CMF Tyrode's solution Rinaldini, L.: An Improved Method for the Isolation and Quantitative Cultivation of Embryonic Cells, Exp Cell Res 16, 477, 1959
Equine Equine, 4-13 yr Hepatocytes Collagenase Type 4: 0.1%
 HBSS Bakala A., Karlik W., and Wiechetek M.: Preparation of Equine Isolated Hepatocytes, Toxicol In Vitro 17(5-6), 615, 2003
Fish Rainbow trout (Oncorhynchus mykiss), male & female,
120-600 g		 Hepatocytes Collagenase Type 2: 0.045%
 Kreb's-Ringer bicarbonate buffer, CF Blair, J.B., Miller, M.R., Pack, D., Barnes, R., Teh, S.J. and Hinton, D.E.: Isolated Trout Liver Cells:Establishing Short-Term Primary Cultures Exhibiting Cell-to-Cell Interactions, In Vitro Cell Dev Biol 26, 237, 1990
Fish Trout, male, 100 - 200 g Hepatocytes Collagenase Type 2: 0.045%
 HBSS with 0.05M HEPES Lipsky, M., Sheridan, T., Bennett, R., and May, E.: Comparison of Trout Hepatocyte Culture on Different Substrates, In Vitro Cell Dev Biol 22, 360, 1986
Fish Rainbow trout, (Salmo gairdneri), male, 150-200 g Hepatocytes Collagenase: 100 u/ml
 HBSS/CMF Klaunig, J., Ruch, R., and Goldblah, P.: Trout Hepatocyte Culture: Isolation and Primary Culture, In Vitro Cell Dev Biol 21, 221, 1985
Fish Trout 100g - 5kg Hepatocytes Hyaluronidase: 0.08%
 See reference Bailey, G., Taylor, M., and Selivonchick, D.: Aflatoxin B1 Metabolism and DNA Binding in Isolated Hepatocytes From Rainbow Trout Salmo gairdner, Carcinogenesis 3, 511, 1982
Fish Trout (Salmo gairdneri), male, 150-200 g
Catfish (Ictalurus punctatus), male, 100 - 150 g		 Hepatocytes Collagenase: 100u/ml
 HBSS Klaunig, J.: Establishment of Fish Hepatocyte Cultures For Use in In Vitro Carcinogenicity Studies, Natl Cancer Inst Monogr 65, 163, 1981
Guinea-Pig Guinea-pig, Hartley, male, 250-300 g Hepatocytes Collagenase Type 2:
Kreb's Ringer bicarbonate buffer Arinze, I. and Kawai, Y.: Adrenergic Regulation of Glycogenolysis in Isolated Guinea-Pig Hepatocytes: Evidence that B2-Receptors Mediate Catecholamine Stimulation of Glycogenolysis, Arch Biochem Biophys 225, 196, 1983
Guinea-Pig Guinea-pig, young Hepatocytes Collagenase: 90 u/ml
 CF EGTA perfusate Reese, J. and Byard, J.: Isolation And Culture of Adult Hepatocytes from Liver Biopsies, In Vitro 17, 935, 1981
Human Human Hepatocytes Collagenase Type 1: 0.05%
 HBSS Reuschel, E, Jilg, W., Seelbach-Goebel, B. and Demi, L.: Comparative Purification and Characterization of Hepatitis B Virus-like Particles Produced by Recombinant Vaccinia Viruses in Human Hepatoma Cells and Human Primary Hepatocytes, PLoS ONE , , 2019
Human Human Hepatocytes Collagenase Type 4: 0.06%
 DMEM Werner, M., Driftmann, S., Kleinehr, K., Kaiser, G., Mathe, Z., Treckmann, J., Paul, A., Skibbe, K., Timm, J., Canbay, A., Gerken, G., Schlaak, J. and Broering, R.: All-In-One: Advanced Preparation of Human Parenchymal and Non-Parenchymal Liver Cells., PLoS ONE 10, e0138655, 2015
Human Human Hepatocytes and non-parenchymal cells Collagenase Type 1: 0.1%
 HBSS Pfeiffer, E., Kegel, V., Zeilinger, K., Hengstler, J., Nussler, A., Seehofer, D. and Damm, G.: Featured Article: Isolation, Characterization, and Cultivation of Human Hepatocytes and Non-Parenchymal Liver Cells., Exp Biol Med (Maywood) 240, 645-56, 2015
Human Human Hepatocytes Collagenase: 0.1-0.15%
 HEPES Lee, S., Schelcher, C., Demmel, M., Hauner, M. and Thasler, W.: Isolation of Human Hepatocytes by a Two-Step Collagenase Perfusion Procedure., J Vis Exp , , 2013
Human Human, fetal Liver hematopoietic Collagenase Type 4: 0.1%
Hyaluronidase: 0.1%
Deoxyribonuclease I: 2 u/ml
 RPMI Vatakis, D., Bristol, G., Kim, S., Levin, B., Liu, W., Radu, C., Kitchen, S. and Zack, J.: Using the BLT Humanized Mouse as a Stem Cell Based Gene Therapy Tumor Model., J Vis Exp 70, e4181, 2012
Human Human Hepatocytes Collagenase Type 1: 0.05%
 Williams E Torok, E., Lutgehetmann, M., Bierwolf, J., Melbeck, S., Dullmann, J., Nashan, B., Ma, P. and Pollok, J.: Primary Human Hepatocytes on Biodegradable Poly(l-lactic acid) Matrices: A Promising Model for Improving Transplantation Efficiency with Tissue Engineering., Liver Transpl 17, 104, 2011
Human Human Hepatic stem cells and heptoblasts Collagenase Type 4: 0.014-0.06%
 various Wauthier, E., Schmelzer, E., Turner, W., Zhang, L., LeCluyse, E., Ruiz, J., Turner, R., Furth, M., Kubota, H., Lozoya, O., Barbier, C., McClelland, R., Yao, H., Moss, N., Bruce, A., Ludlow, J. and Reid, L.: Hepatic Stem Cells and Hepatoblasts: Identification, Isolation, and Ex Vivo Maintenance., Methods Cell Biol 86, 137, 2008
Human Human Hepatocytes Collagenase: 0.05%
 HEPES Pichard L, Raulet E, Fabre G, Ferrini JB, Ourlin JC, and Maurel P: Human hepatocyte culture, Methods Mol Biol 320, 283, 2006
Human Human Hepatocytes Collagenase: 0.05%
 EBSS Hughes, R., Mitry, R., Dhawan, A., Lehec, S., Girlanda, R., Rela, M., Heaton, N., and Muiesan, P.: Isolation of Hepatocytes from Livers from Non-Heart-Beating Donors for Cell Transplantation, Liver Transpl 12, 713, 2006
Human Human Hepatic side population Collagenase: 0.02-0.05%
 HBSS Hussain, S., Strom, S., Kirby, M., Burns, S., Langemeijer, S., Ueda, T., Hsieh, M. and Tisdale, J.: Side Population Cells Derived from Adult Human Liver Generate Hepatocyte-Like Cells In Vitro., Dig Dis Sci 50, 1755, 2005
Human Human, adult Hepatocytes Collagenase: 0.6%
 RPMI 1640 Cho JJ, Joseph B, Sappal BS, Giri RK, Wang R, Ludlow JW, Furth ME, Susick R, and Gupta S.:   Analysis of the functional integrity of cryopreserved human liver cells including xenografting in immunodeficient mice to address suitability for clinical applications, Liver Int 24, 361, 2004
Human Human Hepatocytes Collagenase Type 4: 0.05%
 Williams E Duanmu Z., Locke D., Smigelski J., Wu W., Dahn M., Falany C., Kocarek T., and Runge-Morris M.: Effects of Dexamethasone on Aryl (SULT1A1)- and hydroxysteroid (SULT2A1)-Sulfotransferase Gene Expression in Primary Cultured Human Hepatocytes, Drug Metab Dispos 30(9), 997, 2002
Human Human, fetal Epithelial progenitor Collagenase: 0.03%
 DMEM Malhi, H., Irani, A., Gagandeep, S. and Gupta, S.: Isolation of Human Progenitor Liver Epithelial Cells with Extensive Replication Capacity and Differentiation into Mature Hepatocytes., J Cell Sci 115, 2679, 2002
Human Human
(also rat)		 Hepatocytes Collagenase Type 1: 0.05%
 Leffert's buffer Dandri, M., Burda, M., Torok, E., Pollok, J., Iwanska, A., Sommer, G., et al.: Repopulation of Mouse Liver with Human Hepatocytes and In Vivo Infection with Hepatitis B Virus, Hepatol 33, 981, 2001
Human Human Hepatocytes Collagenase: 0.05%
 Williams E Donato M, Viitala P, Rodriguez-Antona C, Lindfors A, Castell J, Raunio H, Gomez-Lechon M, Pelkonen O: CYP2A5/CYP2A6 expression in mouse and human hepatocytes treated with various in vivo inducers, Drug Metab Dispos 28, 1321-6, 2000
Human Human Hepatocytes Collagenase Type 1: 0.025%
 Williams E Parzefall, W., Erber, E., Sedivy, R., and Schulte-Hermann, R.: Testing for Induction of DNA Synthesis in Human Hepatocyte Primary Cultures by Rat Liver Tumor Promoters, Cancer Res 51, 1143, 1991
Human Human Hepatocytes Collagenase: 0.05%
 HEPES buffer
(see reference)		 Gomez-Lechon, M., Lopez, P., Donato, T., Montoya, A., Larrauri, A., Gimenez, P., Trullenque, R., Fabra, R., and Castell, J.: Culture of Human Hepatocytes From Small Surgical Liver Biopsies: Biochemical Characterization And Comparison With in vivo, In Vitro Cell Dev Biol 26, 67, 1990
Human Human, male, 17-40 yrs. Hepatocytes Collagenase: 0.05%
 HEPES buffer Begue, J., Baffet, G., Campion, J., and Guillouzo, A.: Differential Response of Primary Cultures of Human and Rat Hepatocytes to Aflatoxin B1-Induced Cytotoxicity and Protection by the Hepatoprotective agent(+)-Cyanidanol-3, Biol Cell 63, 327, 1988
Human Human, 4 male kidney donors, age between 17 and 31
(also adult rats, male, SD, 180-200 g)		 Hepatocytes Collagenase: 0.05% & 0.025%
 HEPES
(see reference)		 LeBot, M., Begue, J., Kernaleguen, D., Robert, J., Ratanasavanh, D., Airiau, J., Riche, C., and Guillouzo, A.: Different Cytotoxicity and Metabolism of Doxorubicin, Daunorubicin, Epirubicin, Esorubicin, and Idarubicin in Cultured Human and Rat Hepatocytes, Biochem Pharmacol 37 (20), 3877, 1988
Human Human, 3 human kidney donors, age 20, 23, 25 Hepatocytes Collagenase: 0.05%
 HEPES Gugen-Guillouzo, C., Campion, J., Brissot, P., Glaise, D., Launois, B., Bourel, M., and Guillouzo, A.: High Yield Preparation of Isolated Human Adult Hepatocytes by Enzymatic Perfusion of the Liver, Cell Biol Int Rep 6 (6), 625, 1982
Human Human, 51-75 years old
(also rat, guinea-pig, rabbit, dog)		 Hepatocytes Collagenase: 90 u/ml
 CF EGTA perfusate Reese, J. and Byard, J.: Isolation And Culture of Adult Hepatocytes from Liver Biopsies, In Vitro 17, 935, 1981
Human Human Hepatocytes Trypsin: 0.1%
 HBSS, CMF Kaighn, M.: Human Liver Cells,Tissue Culture Methods / Applications,(Kruse, P., Patterson, M. eds), , 54, 1973
Monkey Monkey, Macaca mulatta, 3-5.5 kg Hepatocytes Collagenase Type 1: 129 u/ml
 DMEM/F12 Weber, A., Groyer-Picard, M.and Dagher, I.: Hepatocyte Transplantation Techniques: Large Animal Models., Methods Mol Biol 481, 83, 2009
Monkey Monkey (Macaca fascicularis), adult, 5-6 Kg Hepatocytes Trypsin: 160 u/ml
 HEPES buffer Ulrich, R., Aspar, D., Cramer, C., Kletzien, R., and Ginsberg, L.: Isolation and Culture of Hepatocytes from the Cynogmolgus Monkey (Macaca fascicularis), In Vitro Cell Dev Biol 26, 815, 1990
Mouse Mouse Hepatocytes Collagenase Type 4: 0.05%
 DMEM Cabral, F., Miller, C., Kudrna, K., Hass, B., Daubendiek, J., Kellar, B. and Harris, E.: Purification of Hepatocytes and Sinusoidal Endothelial Cells from Mouse Liver Perfusion., J Vis Exp , , 2018
Mouse Mouse, 10-13 week Hepatocytes Collagenase Type 2: 0.2 u/ml
 William's Medium E Sewing, S., Boess, F., Moisan, A., Bertinetti-Lapatki, C., Minz, T., Hedtjaern, M., Tessier, Y., Schuler, F., Singer, T. and Roth, A.: Establishment of a Predictive In Vitro Assay for Assessment of the Hepatotoxic Potential of Oligonucleotide Drugs., PLoS ONE 11, e0159431, 2016
Mouse Mouse Hepatocytes Collagenase Type 2: 150 u/ml
 PBS Weerasinghe, S., Park, M., Portney, D. and Omary, M.: Mouse Genetic Background Contributes to Hepatocyte Susceptibility to Fas-mediated Apoptosis., Mol Biol Cell 27, 3005-3012, 2016
Mouse Mouse Hepatic Collagenase Type 4: 0.05%
 HBSS Bigorgne, A., John, B., Ebrahimkhani, M., Shimizu-Albergine, M., Campbell, J. and Crispe, I.: TLR4-Dependent Secretion by Hepatic Stellate Cells of the Neutrophil-Chemoattractant CXCL1 Mediates Liver Response to Gut Microbiota., PLoS ONE 11, e0151063, 2016
Mouse Mouse, 12 week Hepatic stellate Collagenase Type 4: 0.05%
Pronase: 0.04%
 DMEM Mederacke, I., Dapito, D., Affo, S., Uchinami, H. and Schwabe, R.: High-Yield and High-Purity Isolation of Hepatic Stellate Cells from Normal and Fibrotic Mouse Livers., Nat Protoc 10, 305-15, 2015
Mouse Mouse Hepatocytes Collagenase Type 3: 100 u/ml
Collagenase Type 4: 100 u/ml
 DMEM Guenthner, C., Luitje, M., Pyle, L., Molyneux, P., Yu, J., Li, A., Leise, T. and Harrington, M.: Circadian Rhythms of Per2::Luc in Individual Primary Mouse Hepatocytes and Cultures., PLoS ONE 9, e87573, 2014
Mouse Mouse Hepatocytes and sinusoidal endothelial Collagenase Type 4: 0.05%
Deoxyribonuclease I: 0.003%
 HBSS Perepelyuk, M., Terajima, M., Wang, A., Georges, P., Janmey, P., Yamauchi, M. and Wells, R.: Hepatic Stellate Cells and Portal Fibroblasts are the Major Cellular Sources of Collagens and Lysyl Oxidases in Normal Liver and Early After Injury., Am J Physiol Gastrointest Liver Physiol 304, G605, 2013
Mouse Mouse hepatocytes Collagenase Type 4: 100 u/ml
 DMEM Sin, Y., Ballantyne, L., Mukherjee, K., St Amand, T., Kyriakopoulou, L., Schulze, A. and Funk, C.: Inducible Arginase 1 Deficiency in Mice Leads to Hyperargininemia and Altered Amino Acid Metabolism., PLoS ONE 8, e80001, 2013
Mouse Mouse, female, 8-10 week Hepatocytes Collagenase Type 1: 0.08%
 DMEM Severgnini, M., Sherman, J., Sehgal, A., Jayaprakash, N., Aubin, J., Wang, G., Zhang, L., Peng, C., Yucius, K., Butler, J. and Fitzgerald, K.: A Rapid Two-Step Method for Isolation of Functional Primary Mouse Hepatocytes: Cell Characterization and Asialoglycoprotein Receptor Based Assay Development., Cytotechnology 64, 187-95, 2012
Mouse Mouse Hepatocytes & non-parenchymal liver cells Collagenase: 0.03-0.05%
 DMEM Brundert, M., Heeren, J., Merkel, M., Carambia, A., Herkel, J., Groitl, P., Dobner, T., Ramakrishnan, R., Moore, K. and Rinninger, F.: Scavenger Receptor CD36 Mediates Uptake of High Density Lipoproteins in Mice and by Cultured Cells., J Lipid Res 52, 745, 2011
Mouse Mouse Hepatocytes HIS kit: with modifications
 DMEM Kang, H., Okamoto, K., Kim, Y., Takeda, Y., Bortner, C., Dang, H., Wada, T., Xie, W., Yang, X., Liao, G. and Jetten, A.: Nuclear Orphan Receptor TAK1/TR4-Deficient Mice are Protected Against Obesity-Linked Inflammation, Hepatic Steatosis, and Insulin Resistance., Diabetes 60, 177, 2011
Mouse Mouse Hepatacytes Collagenase Type 1: 0.05%
 Williams E Holl, D., Kuckenberg, P., Woynecki, T., Egert, A., Becker, A., Huss, S., Stabenow, D., Zimmer, A., Knolle, P., Tolba, R., Fischer, H. and Schorle, H.: Transgenic Overexpression of Tcfap2c/AP-2gamma Results in Liver Failure and Intestinal Dysplasia., PLoS ONE 6, e22034, 2011
Mouse Mouse CD133+ stem Collagenase Type 4: 0.05%
Pronase: 0.05%
Deoxyribonuclease I: 0.01%
 DMEM/F12 Rountree, C., Ding, W., Dang, H., Vankirk, C. and Crooks, G.: Isolation of CD133+ Liver Stem Cells for Clonal Expansion., J Vis Exp 56, e3183, 2011
Mouse Mouse, male, 12-14 week Hepatocytes Collagenase Type 1: 0.03%
 Williams E Chung, S., Timmins, J., Duong, M., Degirolamo, C., Rong, S., Sawyer, J., Singaraja, R., Hayden, M., Maeda, N., Rudel, L., Shelness, G. and Parks, J.: Targeted Deletion of Hepatocyte ABCA1 Leads to VLDL Triglyceride Over-Production and LDL Hypercatabolism, J Biol Chem 285, 12197, 2010
Mouse Mouse Liver non-parenchymal Collagenase Type 3: 100 u/ml
 HBSS Bosschaerts, T., Guilliams, M., Stijlemans, B., Morias, Y., Engel, D., Tacke, F., Herin, M., De Baetselier, P. and Beschin, A.: Tip-DC Development During Parasitic Infection is Regulated by IL-10 and Requires CCL2/CCR2, IFN-gamma and MyD88 Signaling., PLoS Pathog 6, e1001045, 2010
Mouse Mouse Hepatocytes Collagenase Type 1: 0.075%
 HBSS Shmarakov, I., Fleshman, M., D'Ambrosio, D., Piantedosi, R., Riedl, K., Schwartz, S., Curley, R., von Lintig, J., Rubin, L., Harrison, E. and Blaner, W.: Hepatic Stellate Cells are an Important Cellular Site for β-Carotene Conversion to Retinoid., Arch Biochem Biophys 504, 3-10, 2010
Mouse Mouse, male, adult Hepatocytes Collagenase Type 4: 0.05%
 HBSS Mathijs, K., Kienhuis, A., Brauers, K. J, Jennen, D. , Lahoz, A., Kleinjans, J. and van Delft, J.: Assessing the Metabolic Competence of Sandwich-Cultured Mouse Primary Hepatocytes., Drug Metab Dispos 37, 1305, 2009
Mouse Mouse, male Hepatocytes Collagenase Type 1: 100 u/ml
Elastase: 0.1 u/ml
 Williams E Oliva, J., Bardag-Gorce, F., French, B., Li, J., McPhaul, L., Amidi, F., Dedes, J., Habibi, A., Nguyen, S. and French, S.: Fat10 is an Epigenetic Marker for Liver Preneoplasia in a Drug-Primed Mouse Model of Tumorigenesis., Exp Mol Pathol 84, 102, 2008
Mouse Mouse, 7-10 week Liver endothelial Collagenase: 0.03%
 DMEM Follenzi, A., Benten, D., Novikoff, P., Faulkner, L., Raut, S. and Gupta, S.: Transplanted Endothelial Cells Repopulate the Liver Endothelium and Correct the Phenotype of Hemophilia A Mice., J Clin Invest 118, 935, 2008
Mouse Mouse, 10-12 week Liver sinusoidal endothelial cells Collagenase Type 1: 0.05%
Neutral Protease: 0.025%
 HEPES Beldi, G., Wu, Y., Sun, X., Imai, M., Enjyoji, K., Csizmadia, E., Candinas, D., Erb, L. and Robson, S.: Regulated Catalysis of Extracellular Nucleotides by Vascular CD39/ENTPD1 is Required for Liver Regeneration., Gastroenterol 135, 1751, 2008
Mouse Mouse Liver epithelial progenitor cells Collagenase Type 4: 0.1%
Deoxyribonuclease I: 0.05%
 DMEM Li Wen-Lin, Su Juan, Yao Yu-Cheng, Tao Xin-Rong, Yan Yong-Bi, Yu Hong-Yu, Wang Xin-Min, Li Jian-Xiu, Yang Yong-Ji, Lau Joseph T Y, Hu Yi-Ping: Isolation and characterization of bipotent liver progenitor cells from adult mouse, Stem Cells 24, 322-32, 2006
Mouse Mouse Liver sinusodial endothelial Collagenase: 0.03%
 DMEM Benten Daniel, Follenzi Antonia, Bhargava Kuldeep K, Kumaran Vinay, Palestro Christopher J, Gupta Sanjeev: Hepatic targeting of transplanted liver sinusoidal endothelial cells in intact mice, Hepatol 42, 140-8, 2005
Mouse Mouse Hepatocytes Collagenase Type 1: 0.033%
 Leffert's buffer Jiang Guoqiang, Li Zhihua, Liu Franklin, Ellsworth Kenneth, Dallas-Yang Qing, Wu Margaret, Ronan John, Esau Christine, Murphy Cain, Szalkowski Deborah, Bergeron Raynald, Doebber Thomas, Zhang Bei: Prevention of obesity in mice by antisense oligonucleotide inhibitors of stearoyl-CoA desaturase-1, J Clin Invest 115, 1030-8, 2005
Mouse Mouse, 8-10 week Liver derived stem cells Collagenase Type 1: 0.1%
Neutral Protease: 2.4 u/ml
 HBSS Kotton, D., Fabian, A. and Mulligan, R.: A Novel Stem-Cell Population in Adult Liver with Potent Hematopoietic-Reconstitution Activity., Blood 106, 1574, 2005
Mouse Mouse Hepatocytes Collagenase Type 1: 0.053%
 DMEM/ F-12 Sazani P., Gemignani F., Kang S., Maier M., Manoharan M., Persmark M., Bortner D., and Kole R.: Systemically Delivered Antisense Oligomers Up-regulate Gene Expression in Mouse Tissues, Nat Biotechnol 20, 1228, 2002
Mouse Mouse, male, 4-10 week Hepatocytes Collagenase Type 2: 100 u/ml
 HBSS Lingohr Melissa K, Bull Richard J, Kato-Weinstein Junko, Thrall Brian D: Dichloroacetate stimulates glycogen accumulation in primary hepatocytes through an insulin-independent mechanism, Toxicol Sci 68, 508-15, 2002
Mouse Mouse, male, 8 week Hepatocytes Collagenase Type 1: 0.04%
 Waymouth's medium Hatano E, Brenner DA: Akt protects mouse hepatocytes from TNF-alpha- and Fas-mediated apoptosis through NK-kappa B activation., Am J Physiol/Gastro 282, G1357, 2002
Mouse Mouse Hepatocytes Collagenase: 0.05%
 Williams E Donato M, Viitala P, Rodriguez-Antona C, Lindfors A, Castell J, Raunio H, Gomez-Lechon M, Pelkonen O: CYP2A5/CYP2A6 expression in mouse and human hepatocytes treated with various in vivo inducers, Drug Metab Dispos 28, 1321-6, 2000
Mouse Mouse, 7 week Kupffer cells Collagenase Type 4: 0.05%
 HBSS Angele MK, Knoferl MW, Schwacha MG, Ayala A, Cioffi WG, Bland KI, and Chaudry IH.: Sex steroids regulate pro- and anti-inflammatory cytokine release by macrophages after trauma-hemorrhage, Am J Physiol 277, C35, 1999
Mouse Mouse Nonparenchymal liver Collagenase Type 1: 0.05%
 Hanks Ling W, Lougheed M, Suzuki H, Buchan A, Kodama T, Steinbrecher UP: Oxidized or acetylated low density lipoproteins are rapidly cleared by the liver in mice with disruption of the scavenger receptor class A type I/II gene, J Clin Invest 100, 244-52, 1997
Mouse Mouse, C3H, 6-8 weeks Epithelial Collagenase: 0.10%
 DMEM Lillehaug, J., Mondal, S., and Heidelberger, C.: Establishment of Epithelial Cell Lines from Mouse Regenerating Liver, In Vitro 15, 910, 1979
Mouse Mouse, 20-30 g Parenchymal and non-parenchymal Hyaluronidase: 0.1%
 Hank's w/ Insulin, CMF Crisp, D., and Pogson, C.: Glycolytic and Gluconeogenic Enzyme Activities in Parenchymal and Non-parenchymal Cells from Mouse Liver, Biochem J 126, 1009, 1972
Porcine Porcine, mini-pig, 13 kg Hepatocytes Collagenase Type 4: 0.05%
Neutral Protease: 0.84%
Deoxyribonuclease I: see reference
 Williams E Meng, F., Chen, Z., Han, M., Hu, X., He, X., Liu, Y., He, W., Huang, W., Guo, H. and Zhou, P.: Porcine Hepatocyte Isolation and Reversible Immortalization Mediated by Retroviral Transfer and Site-Specific Recombination., World J Gastroenterol 16, 1660, 2010
Porcine Porcine, Yorkshire, male, 21 day Hepatocytes Collagenase Type 1: 0.07%
 Williams E Terner, M., Gilmore, W.J., Lou, Y. and Squires, E.J.: The Role of CYP2A and CYP2E1 in the Metabolism of 3-Methylindole in Primary Cultured Porcine Hepatocytes., Drug Metab Dispos 34, 848, 2006
Porcine Porcine, Chinese mini pig, 6-10 kg Hepatocytes Collagenase Type 4: 125 u/ml
 Williams E Li, J, Li, L., Chao, H., Yang, Q., Liu, X., Sheng, J., Yu, H. and Huang, J: Isolation and Short Term Cultivation of Swine Hepatocytes for Bioartificial Liver Support System., Hepatobiliary Pancreat Dis Int 4, 249, 2005
Porcine Porcine, one week Hepatocytes Collagenase Type 4: 0.05%
 RPMI 1640 Wang Y., Liu H., Guo H., Wen H., and Liu J.: Primary Hepatocyte Culture in Collagen Gel Mixture and Collagen Sandwich, World J Gastroenterol 10, 699, 2004
Porcine Porcine, male, 2-3 week Hepatocytes Collagenase: 0.05%
 DMEM Raman Priya, Donkin Shawn S, Spurlock Michael E: Regulation of hepatic glucose metabolism by leptin in pig and rat primary hepatocyte cultures, Am J Physiol Regul Integr Comp Physiol 286, R206-16, 2004
Porcine Porcine, adult Hepatocytes Collagenase: 0.05%
Neutral Protease:
MEM Zhou X, Liu L, Kano J, Mukaiyama T, and Tokiwa T.: Isolation and Cultivation of Porcine Hepatocytes for Extracorporeal Artificial Liver Support System, Chin Med J 114, 946, 2001
Porcine Porcine, male, 6-40 kg Hepatocytes Collagenase: 0.8%
 PBS Gerlach, J., Brombacher, J., Smith, M., Neuhaus, P.: High Yield Hepatocyte Isolation from Pig Livers for Investigation of Hybrid Liver Support Systems: Influence of Collagenase Concentration and Body Weight, J Surg Res 62 (1), 85, 1996
Rabbit Rabbit, New Zealand white Hepatocytes Collagenase: 90 u/ml
 CF EGTA perfusate Reese, J. and Byard, J.: Isolation And Culture of Adult Hepatocytes from Liver Biopsies, In Vitro 17, 935, 1981
Rat Rat, SD, 500-700g Hepatocytes and sinusoidal endothelial Collagenase Type 4: 0.05%
Deoxyribonuclease I: 0.003%
 HBSS Perepelyuk, M., Terajima, M., Wang, A., Georges, P., Janmey, P., Yamauchi, M. and Wells, R.: Hepatic Stellate Cells and Portal Fibroblasts are the Major Cellular Sources of Collagens and Lysyl Oxidases in Normal Liver and Early After Injury., Am J Physiol Gastrointest Liver Physiol 304, G605, 2013
Rat Rat, adult Hepatocytes Collagenase Type 2: 1000 U
 William's Shen, L., Hillebrand, A., Wang, D. and Liu, M.: Isolation and Primary Culture of Rat Hepatic Cells., J Vis Exp 64, e3917, 2012
Rat Rat, adult Portal fibroblasts Collagenase Type 2: 0.3%
 DMEM/F-12 Wen, J., Olsen, A., Perepelyuk, M. and Wells, R.: Isolation of Rat Portal Fibroblasts by In Situ Liver Perfusion., J Vis Exp 64, e3669, 2012
Rat Rat Hepatocytes Collagenase Type 2: 0.05%
 DMEM Budick-Harmelin, N., Anavi, S., Madar, Z. and Tirosh, O.: Fatty Acids-Stress Attenuates Gluconeogenesis Induction and Glucose Production in Primary Hepatocytes., Lipids Health Dis 11, 66, 2012
Rat Rat, SD, 240-320g Hepatocytes HIS kit: per instructions
 Waymouth's MB Pillai, V., and Mehvar, R.: Inhibition of NADPH-Cytochrome P450 Reductase by Tannic Acid in Rat Liver Microsomes and Primary Hepatocytes: Methodological Artifacts and Application to Ischemia-Reperfusion Injury, J Pharm Sci 100, 3495, 2011
Rat Rat Hepatocytes and SEC's Collagenase Type 1: 0.1-0.2%
 RPMI Gopalakrishnan, S. and Harris, E.: In Vivo Liver Endocytosis Followed by Purification of Liver Cells by Liver Perfusion., J Vis Exp 57, e3138, 2011
Rat Rat, Wistar, female Hepatic stellate cells Collagenase Type 1: 0.025-0.1%
Pronase: 0.025-0.13%
 DMEM Zvibel, I., Atias, D., Phillips, A., Halpern, Z. and Oren, R.: Thyroid Hormones Induce Activation of Rat Hepatic Stellate Cells Through Increased Expression of p75 Neurotrophin Receptor and Direct Activation of Rho., Lab Invest 90, 674, 2010
Rat Rat, SD Hepatic stellate cells Pronase: 0.02%
Collagenase: see reference
 Medium 199 Handy, J., Saxena, N., Fu, P., Lin, S., Mells, J., Gupta, N. and Anania, F.: Adiponectin Activation of AMPK Disrupts Leptin-Mediated Hepatic Fibrosis via Suppressors of Cytokine Signaling (SOCS-3)., J Cell Biochem 110, 1195, 2010
Rat Rat, SD, 250-300g Hepatocytes HIS kit: per instructions
 Krebs-Henseleit Parasrampuria, R. and Mehvar, R.: Dose-Dependent Inhibition of Transporter-Mediated Hepatic Uptake and Biliary Excretion of Methotrexate by Cyclosporine A in an Isolated Perfused Rat Liver Model., J Pharm Sci 99, 5060, 2010
Rat Rat, SD Hepatocytes Collagenase: 0.05%
 HBSS Chung, C., Shugrue, C., Nagar, A., Doll, J., Cornwell, M., Gattu, A., Kolodecik, T., Pandol, S. and Gorelick, F.: Ethanol Exposure Depletes Hepatic Pigment Epithelium-Derived Factor, a Novel Lipid Regulator., Gastroenterol 136, 331, 2009
Rat Rat, Lewis, male Hepatocytes Collagenase Type 2: 0.1%
 DPBS Bettinger, C., Kulig, K., Vacanti, J., Langer, R. and Borenstein, J.: Nanofabricated Collagen-Inspired Synthetic Elastomers for Primary Rat Hepatocyte Culture., Tissue Eng Part A 15, 1321, 2009
Rat Rat, 200-300 g Hepatocytes Collagenase Type 1: 0.067%
 HBSS Mula, N., Cubero, F., Codesal, J., de Andres, S., Escudero, C., Garcia-Barrutia, S., Millan, I., Arahuetes, R. and Maganto, P.: Survival of Allogeneic Hepatocytes Transplanted into the Thymus., Cells Tissues Organs 188, 270, 2008
Rat Rat, SD, male, 230-250g Hepatocytes Collagenase Type 2: 0.1%
 HEPES Doleh, L. and Romani, A.: Biphasic Effect of Extra-Reticular Mg2+ on Hepatic G6P Transport and Hydrolysis., Arch Biochem Biophys 467, 283, 2007
Rat Rat, Lewis, 150-200g Hepatocytes Collagenase Type 2: 0.05%
 Williams E Smith, M. and Mooney, D.: Hypoxia Leads to Necrotic Hepatocyte Death., J Biomed Mater Res A 80, 520, 2007
Rat Rat, SD, 180-200g Hepatocytes Collagenase Type 1: 0.1%
 see reference Charbonneau, A., Unson, C. and Lavoie, J.: High-Fat Diet-Induced Hepatic Steatosis Reduces Glucagon Receptor Content in Rat Hepatocytes: Potential Interaction with Acute Exercise., J Physiol 579, 255, 2007
Rat Rat, Wistar, 270-330g Hepatocytes Collagenase Type 2: 0.033%
 Williams E Li, W., Ralphs, K., Slack, J. and Tosh, D.: Keratinocyte Serum-Free Medium Maintains Long-Term Liver Gene Expression and Function in Cultured Rat Hepatocytes by Preventing the Loss of Liver-Enriched Transcription Factors., Int J Biochem Cell Biol 39, 541-54, 2007
Rat Rat, SD, 500-750 g Portal fibroblasts Collagenase: 0.03%
Pronase: 0.033%
Hyaluronidase: 0.036%
 DMEM/F-12 Li, Z., Dranoff, J., Chan, E., Uemura, M., Sevigny, J.and Wells, R.: Transforming Growth Factor-beta and Substrate Stiffness Regulate Portal Fibroblast Activation in Culture., Hepatol 46, 1246-56, 2007
Rat Rat, Fisher, E14 Hepatocytes Collagenase Type 1: 0.22%
 HBSS Isabel Zvibel, Miri Bronstein, Einav Hubel, Ella Bar-Lev, Zamir Halpern, Ran Oren: Isolation, characterization and culture of Thy1-positive cells from fetal rat livers, World J Gastroenterol 12, 3841-7, 2006
Rat Rat, Lewis, female, 150-200 g Hepatocytes Collagenase: see references
 DMEM Sosef MN, Baust JM, Sugimachi K, Fowler A, Tompkins RG, and Toner M.: Cryopreservation of isolated primary rat hepatocytes: enhanced survival and long-term hepatospecific function, Ann Surg 241, 125, 2005
Rat Rat Liver sinusodial endothelial Collagenase: 0.03%
 DMEM Benten Daniel, Follenzi Antonia, Bhargava Kuldeep K, Kumaran Vinay, Palestro Christopher J, Gupta Sanjeev: Hepatic targeting of transplanted liver sinusoidal endothelial cells in intact mice, Hepatol 42, 140-8, 2005
Rat Rat, Wistar, 200-300 g Hepatocytes Collagenase Type 2: 0.05%
 Williams E Putz G, Schmider W, Nitschke R, Kurz G, Blum HE: Synthesis of phospholipid-conjugated bile salts and interaction of bile salt-coated liposomes with cultured hepatocytes, J Lipid Res 46, 2325-38, 2005
Rat Rat, adult Hepatocytes Collagenase Type 1: 0.1%
Pronase: 1%
Deoxyribonuclease I: 0.007%
 Williams E Jensen C., Jauho E., Santoni-Rugiu E., Holmskov U., Teisner B., Tygstrup N., and Bisgaard H.: Transit-amplifying Ductular (oval) Cells and Their Hepatocytic Progeny are Characterized by a Novel and Distinctive Expression of Delta-like Protein/preadipocyte Factor 1/fetal Antigen 1, Am J Pathol 164(4), 1347, 2004
Rat Rat, SD, male, 200-250 g Hepatocytes Collagenase: 0.05%
 DMEM Raman Priya, Donkin Shawn S, Spurlock Michael E: Regulation of hepatic glucose metabolism by leptin in pig and rat primary hepatocyte cultures, Am J Physiol Regul Integr Comp Physiol 286, R206-16, 2004
Rat Rat, Wistar Hepatocytes Collagenase Type 4: 0.05%
 RPMI 1640 Wang Y., Liu H., Guo H., Wen H., and Liu J.: Primary Hepatocyte Culture in Collagen Gel Mixture and Collagen Sandwich, World J Gastroenterol 10, 699, 2004
Rat Rat, Wistar, 220-270g Hepatocytes Collagenase Type 1: 200 u/ml
 DMEM Annaert, P., Turncliff, R., Booth, C., Thakker, D. and Brouwer, K.: P-Glycoprotein-Mediated In Vitro Biliary Excretion in Sandwich-Cultured Rat Hepatocytes., Drug Metab Dispos 29, 1277-83, 2001
Rat Rat, SD, male Hepatocytes Collagenase Type 2: 0.025%
 Williams E Burczynski, M., McMillian, M., Parker, J., Bryant, S., Leone, A., Grant, E., Thorne, J., Zhong, Z., Zivin, R. and Johnson, M.: Cytochrome P450 Induction in Rat Hepatocytes Assessed by Quantitative Real-Time Reverse-Transcription Polymerase Chain Reaction and the RNA Invasive Cleavage Assay., Drug Metab Dispos 29, 1243, 2001
Rat Rat, SD, male, 230-250 g Hepatocytes Collagenase Type 1: 0.1%
 HBSS Kuddus, R., Nalesnik, M., Subbotin, V., Rao, A., and Gandhi, C.: Enhanced Sythesis and Reduced Metabolism of Endothelin-1 (ET-1) by Hepatocytes - An Important Mechanism of Increased Endogenous Levels of ET-1 in Liver Cirrhosis, J Hepatol 33, 725, 2000
Rat Rat, male, 3 week Hepatocytes Collagenase Type 4: 0.02%
 Williams E Low-Baselli A., Hufnagl K., Parzefall W., Schulte-Hermann R., and Grasl-Kraupp B.: Initiated Rat Hepatocytes in Primary Culture: A Novel Tool to Study Alterations in Growth Control During the First Stage of Carcinogenesis, Carcinogenesis 21, 79, 2000
Rat Rat, SD, male, 450-500 g Stellate Protease: 0.02%
 HBSS Gabriel, A., Kuddus, R., Rao, A., and Gandhi, C.: Down-Regulation of Endothelin Receptors by Transforming Growth Factor B1 in Hepatic Stellate Cells, J Hepatol 30, 440, 1999
Rat Rat, Wistar, male Hepatocytes Collagenase Type 2: 120 u/ml
 HBSS Wolz E, Liechti H, Notter B, Oesterhelt G, Kistler A: Characterization of metabolites of astaxanthin in primary cultures of rat hepatocytes, Drug Metab Dispos 27, 456-62, 1999
Rat Rat, Lewis, adult Hepatocytes Collagenase: 60 u/ml
 HEPES Matsuura T, Gad MZ, Harrison EH, and Ross AC: Lecithin:retinol acyltransferase and retinyl ester hydrolase activities are differentially regulated by retinoids and have distinct distributions between hepatocyte and nonparenchymal cell fractions of rat liver, J Nutr 127, 218, 1997
Rat Rat Hepatocytes Collagenase: 0.04% - 0.06%
 PBS Alston-Smith, J and Pertoft, H: Isolation of Liver Cells: a System for Obtaining Pure Cells in Monolayer Cultures from a Single Rat Liver,Cell & Tissue Culture: Laboratory Procedures Vol. 1,Doyle, A., Griffiths, J., and Newell, D., John Wiley and Sons, Ltd., 12B:14.1, 1995
Rat Rat Hepatocytes Collagenase: 0.05%
 DMEM Matsumoto, K and Nakamura, T: Techniques for the Isolation and Cultivation of Hepatocytes using Collagenase,Cell & Tissue Culture: Laboratory Procedures Vol. 1,Doyle, A., Griffiths, J., and Newell, D., John Wiley and Sons, Ltd., 12B:16.1, 1995
Rat Rat, SD, male, 200 g Hepatocytes Collagenase: 0.05%
 Medium 199 Rana, B., Mischoulon, D., Xie, Y., Bucher, N., and Farmer, S.: Cell-Extracellular Matrix Interactions Can Regulate the Switch between Growth and Differentiation in Rat Hepatocytes: Reciprocal Expression of C/EBPa and Immediate-Early Growth Response Transcription Factors, Mol Cell Biol 14 (9), 5858, 1994
Rat Rat, Wistar, adult, male, 100-125 g Hepatocytes Collagenase Type 2: 0.05%
 Serum-free medium Liu, J., McKim, J., Liu, Y., and Klaassen, C.: Effects of Butyrate Homologes on Metallothionein Induction in Rat Primary Hepatocyte Cultures, In Vitro Cell Dev Biol 28, 320, 1992
Rat Rat, SD, male, 250-350 g Hepatocytes Collagenase Type 4: 200 u/ml
 RPMI 1640 Dixit, V., Arthur, M., and Gitnick, G.: Repeated Transplantation of Microencapsulated Hepatocytes for Sustained Correction of Hyperbilirubinemia in Gunn Rats, Cell Transplant 1, 275, 1992
Rat Rat, Fisher Epithelial Collagenase: 0.05%
Neutral Protease: 0.48%
Hyaluronidase: 0.075%
 Eagles's MEM Blouin, M., and Marceau, N.: Primary Culture of Fetal Rat Liver Bipotential Progenitor Cells, J Tiss Cul Meth 13, 117, 1991
Rat Rat, SD, 8 - 12 day Parenchymal hepatocytes Collagenase Type 4: 80 u/ml
 HBSS Davila, J., Reddy, C., Davis, P. and Acosta, D.: Toxicity Assessment of Papaverine Hydrochloride and Papaverine-Derived Metabolites in Primary Cultures of Rat Hepatocytes, In Vitro Cell Dev Biol 26, 515, 1990
Rat Rat, SD, male, 250-350 g Hepatocytes Collagenase Type 2: 0.05%
 HBSS, CMF Liu, J., Kershaw, W., and Klaassen, C.: Rat Primary Hepatocyte Cultures are a Good Model for Examining Metallothionein-Induced Tolerance to Cadmium Toxicity, In Vitro Cell Dev Biol 26, 75, 1990
Rat Rat, Wistar, male, 200 g Parenchymal Collagenase Type 1:
HEPES, modified Kindberg, G., Gudmundsen, O., and Berg, T.: The Effect of Vandate on Receptor-mediated Endocytosis of Asialoorosomucoid in Rat Liver Parenchymal Cells, J Biol Chem 265, 8999, 1990
Rat Rat, SD, male, 175-225 g Hepatocytes Collagenase:
Kreb's Ringer bicarbonate buffer Reddy, S., Amick, G., Cooper, R., and Damun, Z.: Insulin Stimulates the Activity of a Protamine in Isolated Rat Hepatocytes, J Biol Chem 265, 7748, 1990
Rat Rat, SD, neonatal, 8 - 10 days Hepatocytes Collagenase Type 4: 0.05%
 Hanks' BSS, CF Davila, J., Lenherr, A., and Acosta, D.: Protective Effect of Flavonoids on Drug-Induced Hepatotoxicity In Vitro, Toxicology 57, 267, 1989
Rat Rat, Fisher, male, 125-250 g Hepatocytes Collagenase Type 1: 0.5%
 RPMI 1640 Chan, K., Kost, D., and Michalopoulos, G.: Multiple Sequential Periods of DNA Synthesis and Quiescence in Primary Hepatocyte Cultures Maintained on the DMSO-EGF On/Off Protocol, J Cell Physiol 141, 584, 1989
Rat Rat, Fischer 344, male, 14 months Hepatocytes Collagenase: 0.05%
 Ringer's biocarbonate buffer Nagy, I., Ohno-Iwashita, Y., Ohta, M., Nagy, V., Kitani, K., Ando, S., and Imahori, K.: Effect of Perfringolysin O on the Lateral Diffusion Constant of Membrane Proteins of Hepatocytes as Revealed by Fluorescence Recovery After Photobleaching, Biochim Biophys Acta 939, 551, 1988
Rat Rat, SD, male, 200-300 g Hepatocytes Collagenase: 0.05%
 HEPES Voss, A. and Sprecher, H.: Metabolism of 6,9,12-Octadecatetraenoic and 6,9,12,15-Octadecatetraenoic Acid, Biochim Biophys Acta 958, 153, 1988
Rat Rat, Wistar, male,3 month, 200 g Parenchymal
Endothelial
Kupffer		 Collagenase Type 1: 0.05%
 Krebs Henseleit Kuiper, J., Zijlstra, F., Kamps, J, and vanBerkel, T.: Identification of Prostaglandin D2 as the Major Eiconsanoid from Liver Endothelial and Kupffer Cells, Biochim Biophys Acta 959, 143, 1988
Rat Rat, Wistar, male, 200 - 300 g Parenchymal
Kupffer		 Collagenase Type 2: 0.05%
 HBSS with CaCl2 Cai, H., He, Z., and Ding, Y.: Effects of Monocyte Macrophages Stimulation on Hepatic Lipoprotein Receptors, Biochim Biophys Acta 958, 334, 1988
Rat Rat, Wistar, female, 17 days Hepatocytes Collagenase: 0.05%
 HBSS Cotariu, D., Barr-Nea, L., Papo, N., and Zaidman, J.: Induction of gamma-Glutamyl Transferase by Dexamethasone in Cultured Rat Hepatocytes, Enzyme 40, 212, 1988
Rat Rat, CD strain, albino, male, 140 -180 g Hepatocytes and Nonparenchymal Pronase: 0.1%
 HEPES buffer with calcium Braun, L., Mead, J., Panzica, M., Mikumo, R., Bell, G., and Fausto, N.: Transforming Growth Factor B mRNA Increases During Liver Regeneration:A Possible Paracrine Mechanism Of Growth Regulation, Proc Natl Acad Sci U S A 85, 1539, 1988
Rat Rat, 250-350 g
(also guinea-pig & rabbit)		 Hepatocytes Collagenase Type 2: 166 u/ml
 DMEM Chang, T., and Chang, C.: Hepatic Uptake of Asialoglycoprotein is Different Among Mammalian Species Due to Different Receptor Distribution, Biochim Biophys Acta 942, 57, 1988
Rat Rat, SD, male, 180-200 g Hepatocytes Collagenase: 0.05%
 MEM Gupta, C., Hattori, A., Betschart, J., Virji, M., and Shinozuka, H.: Modulation of Epidermal Growth Factor Receptors in Rat Hepatocytes by Two Liver-Promoting Regimens, a Choline-Deficient and a Phenobarbital Diet, Cancer Res 48, 1162, 1988
Rat Rat, Fisher 344, 150-200 g Bile ductular epithelial Collagenase Type 1: 220 u/ml
 L-15 Mathis, G., Walls, S., and Sirica, A.: Biochemical Characteristics of Hyperplastic Rat Bile Ductular Epithelial Cells Cultured "on Top" and "Inside" Different Extracellular Matrix Substitutes, Cancer Res 48, 6145, 1988
Rat Rat, Wistar, male, 260-310 g Hepatocytes Collagenase Type 1: 0.05%
 HEPES McAbee DD and Weigel PH: ATP-dependent inactivation and reactivation of constitutively recycling galactosyl receptors in isolated rat hepatocytes, Biochemistry 27, 2061, 1988
Rat Rat, SD, male, 350-400 g Hepatocytes Collagenase Type 2:
HBSS Rodriguez de Turco, E., and Spitzer, J.: Kinetics of Diacylglycerol Accumulation in Response to Vasopressin Stimulation in Hepatocytes of Continuously Endotoxaemic Rats, Biochem J 253, 73, 1988
Rat Rat, SD, male, 350 - 450 g Lipocytes
Kupffer
Sinusoidal endothelial		 Collagenase: 0.015%
 DMEM/Ham's F-12 Friedman, S. and Roll, F.: Isolation and Culture of Hepatic Lipocytes,Kupffer Cells, and Sinusoidal Endothelial Cells by Density Gradient Centrifugation with Stractan, Anal Biochem 161, 207, 1987
Rat Rat, SD, male Hepatocytes Collagenase: 100 - 200 µg/g body weight
 Eagle's
Eagle's w/HEPES
HBSS		 Oka, J. and Weigel, P.: Monensin Inhibits Ligand Dissociation Only Transiently and Partially and distinguishes two galactosyl receptor pathways in isolated rat hepatocytes, J Cell Physiol 133, 243, 1987
Rat Rat, Fischer, male, 150-250 g Hepatocytes Collagenase Type 1: 125 - 250 u/ml
 MEM
(see reference)		 Francavilla, A., Ove, P., Polimeno, L., Sciascia, C., Coetzee, M., and Starzi, T.: Epidermal Growth Factor and Proliferation in Rat Hepatocytes in Primary Culture Isolation at Different Times after Partial Hepatectomy, Cancer Res 46, 1318, 1986
Rat Rat, SD, male, 200-250 g Hepatocytes Collagenase: 0.05%
 HBSS Kreamer, B., Staecker, J., Sawada, N., Sattler, G., Hsia, M., and Pitot, H.: Use of Low-Speed, Iso-Density Percoll Centrifugation Method to Increase the Viability of Isolated Rat Hepatocyte Preparations, In Vitro Cell Dev Biol 22 (4), 201, 1986
Rat Rat, SD, female, 100-180 g Hepatocytes Collagenase: 0.5%
 Krebs Ringer bicarbonate buffer Schwarz, K., Lanier, S., Carter, E., Homcy, C., and Graham, R.: Rapid Reciprocal Changes in Adrenergic Receptors in Intact Isolated Hepatocytes During Primary Cell Culture, Mol Pharmacol 27, 200, 1985
Rat Rat, Lewis, female, 200-250 g Hepatocytes, Kupffer, endothelial Collagenase: 0.05%
 Gey's BSS Holstege A, Leser HG, Pausch J, Gerok W: Uridine catabolism in Kupffer cells, endothelial cells, and hepatocytes, Eur J Biochem 149, 169-73, 1985
Rat Rat, SD, male, 270 - 320 g Hepatocytes Collagenase: 0.04%
 Bicarbonate buffer with calcium added Brass, E., Garrity, M., and Robertson, R.: Inhibition of Glucagon-Stimulated Hepatic Glycogenolysis by E-Series Prostaglandins, F.E.B.S. Lett. 169, 293, 1984
Rat Rat, Wistar, adult, male, 150-200 g Hepatocytes Trypsin: 0.005%
 Williams E Okumura, T. and Saito, K.: Degradation of Prostaglandin E2 in a Primary Culture of Adult Rat Hepatocytes, J. Biochem. 96, 429, 1984
Rat Rat, Fischer, Lewis and SD, male 10-18 months Epithelial Trypsin: 0.05%
 HBSS CMF Herring, A., Raychaudhuri, R., Kelley, S., and Iybe, P.: Repeated Establishment of Diploid Epithelial Cell Cultures from Normal and Partially Hepatectomized Rats, In Vitro 19, 576, 1983
Rat Rat, Wistar, male, 3 month old Endothelial
Kupffer
Parenchymal		 Collagenase: 0.05%
Pronase: 0.25%
 HBSS Nagelkenke, J., Barto, K., and VanBerkel, T.: In vivo and in vitro uptake and degradation of acetylated low density lipoprotein by rat liver endothelial, Kupffer, and parenchymal cells, J Biol Chem 258, 12221, 1983
Rat Rat, SD, male/female, 250-300 g Hepatocytes Collagenase: 100 u/ml
 Krebs Henseleit bicarbonate buffer Studer, R. and Borle, A.: Differences between Male and Female Rats in the Regulation of Hepatic Glycogenolysis. The Relative Role of Calcium and cAMP in Phosphorylase Activation by Catecholamines, J Biol Chem 257, 7987, 1982
Rat Rat, Wistar, 12 day Epithelial Trypsin: 0.05%
 HBSS, CMF Malan-Shibley, L., and Iype, P.: Influence of Cultures on Cell Morphology/Tyrosine Aminotransferase Levels, Exp Cell Res 131, 363, 1981
Rat Rat, Zucker, obese Hepatocytes Collagenase Type 2: 0.30%
 Dulbecco-Vogt arginine free Eagle's Goldstein, A., Palmer, J., and Johnson, P.: Primary Cultures of Fetal Hepatocytes from the Genetically Obese Zucker Rat: Protein Synthesis, In Vitro 17, 651, 1981
Rat Rat, SD Hepatocytes Collagenase: 90 u/ml
 CF EGTA perfusate Reese, J. and Byard, J.: Isolation And Culture of Adult Hepatocytes from Liver Biopsies, In Vitro 17, 935, 1981
Rat Rat, Wistar, female, fetus Hepatocytes Collagenase: 0.025%
 HEPES buffer Gugen-Guillouzo, C., Tichonicky, L., Szajnert, M., and Kruh, J.: Changes in Some Chromatin and Cytoplastic Enzymes of Perinatal Rat Hepatocytes, In Vitro 16, 1, 1980
Rat Rat, SD, male, 250-300 g Parenchymal Collagenase Type 2: 0.05%
 Kreb's Henseleit bicarbonate buffer Yamada, S., Otto, P., Kennedy, D., and Whayne, T.: The Effects of Dexamethasone on Metabolic Activity of Hepatocytes in Primary Monolayer Culture, In Vitro 16, 559, 1980
Rat Rat, Wistar, male, 180-210 g Hepatocytes Hyaluronidase: 0.02%
 Kreb's buffer De Gerlache, J., Lans, M., Taper, H., and Roberfroid, M.: Separate Isolation of Cells from Nodules and Surrounding Parenchyma of the Same Precancerous Rat Liver: Biochemical and Cytochemical Characterization, Toxicology 18, 225, 1980
Rat Rat, SD, 7-10 day Liver Collagenase: 0.05%
 HBSS modified (see reference) Acosta, D., Anuforo, D., and Smith, R: Preparation of Primary Monolayer Cultures of Postnatal Rat Liver Cells, J Tiss Cul Meth 6, 35, 1980
Rat Rat, Wistar, male, 200-250 g Hepatocytes Hyaluronidase: 460 u/ml
 Saline Poli, G., Gravela, E., Albano, E., and Dianzani, M.: Studies on Fatty Liver with Isolated Hepatocytes: II., The Action of Carbon Tetrachloride on Lipid Peroxidation, Protein and Triglyceride Synthesis and Secretion, Exp Mol Pathol 30, 116, 1979
Rat Rat, SD, female, 100-150 g Hepatocytes Collagenase Type 1: 0.065%
 DMEM
(see reference)		 Davis, R., Engelhorn, S., Pangburn, S., Weinstein, D., and Steinberg, D.: Very Low Density Lipoprotein Synthesis and Secretion by Cultured Rat Hepatocytes, J Biol Chem 254 (6), 2010, 1979
Rat Rat
(also chicken)		 Hepatocytes Collagenase Type 4: 6000 units
Collagenase Type 3 & 4:
HEPES Weigel, P., Schnaar, R., Kuhlenschmidt, M., Schmell, E., Lee, R., Lee, Y., and Roseman, S.: Adhesion of Hepatocytes to Immobilized Sugars, J Biol Chem 254 (21), 10830, 1979
Rat Rat, SD, male, 200 g Hepatocytes Collagenase Type 1: 100 u/ml
 Buffers 1 & 2
(see reference)		 Rubin, K., Kjellen, L., and Oslashbrink, B.: Intercellular Adhesion between Juvenile Liver Cells.. A Method to Measure the Formation of Stable Lateral Contacts Between Cells Attached to a Collagen Gel, Exp Cell Res 109, 413, 1977
Rat Rat (WAG/RIJ), female, 24, 30, and 37 months Parenchymal Collagenase Type 1: 0.05% - 0.06%
 HEPES buffer VanBezodijen, C., Grell, T., and Knook, D.: Effect of Age on Protein Synthesis by Isolated Liver Parenchymal Cells, Mech Ageing Dev 6, 293, 1977
Rat Rat, Fisher, adult, male Hepatocytes Collagenase Type 1: 100 u/ml
 HBSS
(see reference)		 Williams, G., Bermudez, E., and Scaramuzzino, D.: Rat Hepatocytes Primary Cell Cultures III. Improved Dissociation and Attachment Techniques and the Enchancement of Survival by Culture Medium, In Vitro 13 (12), 809, 1977
Rat Rat , Wistar, male, 250-300 g Hepatocytes Collagenase: 0.05%
 Hank's solution, CF Gravela, E., Poli, G., Albano, E., and Dianzani, M.: Studies of Fatty Liver with Isolated Hepatocytes, Exp Mol Pathol 27, 339, 1977
Rat Rat, Fischer, male, adult, 170-265 g Hepatocytes Collagenase: 0.05%-0.10%
 Williams E Laishes, B., and Williams, G.: Conditions Affecting Primary Cell Cultures of Functional Adult Rat Hepatocytes. I.. The Effect of Insulin, In Vitro 12, 521, 1976
Rat Rat, SD, male, 200-250 g Hepatocytes Collagenase Type 1: 0.05%
 MEM Witters, L., Alberico, L., and Acruch, J.: Insulin Regulation of Glycogen Synthase in the Isolated Rat Hepatocyte, Biochem Biophys Res Commun 69 (4), 997, 1976
Rat Rat, Fischer, adult, 200-250 g Epithelial Hyaluronidase: 0.0075%
 KCl-NaCl HEPES Buffer Williams, G., and Gunn, J.: Long-Term Culture of Adult Rat Liver Epithelial Cells, Exp Cell Res 89, 139, 1974
Rat Rat, neonate, 3 day Hepatocytes Trypsin: 0.25%
 PBS, CMF Bausher, J., and Schaeffer, W.: A Diploid Rat Liver Cell Culture. 1. Characterization and Sensitivity to Aflatoxin B1, In Vitro 9, 286, 1974
Rat Rat, SD, albino, male Parenchymal Hyaluronidase: 0.1%
 Hank's solution, CMF Bonney, R., Becker, J., Walker, P., and Potter, V.: Primary Monolayer Cultures of Adult Rat Liver Parenchymal Cells Suitable for Study of the Regulation of Enzyme Synthesis, In Vitro 9, 399, 1974
Rat Rat, embryos, 1-3 days Hepatocytes Hyaluronidase: 0.10%
 HBSS Gerschenson, L., Berliner, J., and Davidson, M.: The Isolation and Culture of Liver Cells, Vol. 32,, , 733, 1974
Rat Rat, SD, adult, male, 200-250 g Parenchymal Hyaluronidase: 0.10%
 HBSS, CF Howard, R., Lee, J., and Pesch, L.: The fine structure, potassium content, and respiratory activity of isolated rat liver parenchymal cells prepared by improved enzymatic techniques, J Cell Biol 57, 642, 1973
Rat Rat, SD, adult, male, 180-300 g Parenchymal Collagenase Type 1: 0.05%
 Hank's solution, CF Bissell, D., Hammaker, L., and Meyer, U.: Parenchymal Cells from Adult Rat Liver in Nonproliferating Monolayer Culture. I. Functional Studies, J Cell Biol 59, 722, 1973
Rat Rat, Wistar, 200-250 g Hepatocytes Hyaluronidase: 0.10%
 HBSS, CF Berg, T., Boman, D., and Seglen, P.O.: Induction of Tryptophan Oxygenase in Primary Rat Liver Cell Suspensions by Glucocorticoid Hormone, Exp Cell Res 72, 571, 1972
Rat Rat, SD, female, 130-160 g Hepatocytes Hyaluronidase: 0.08%
 HBSS, CF Johnson, M., Das, N., Butcher, F., and Fain, J.: The Regulation of Gluconeogenesis in Isolated Rat Liver Cells by Glucagon, Insulin, Dibutyryl Cyclic Adenosine Monophosphate, and Fatty Acids, J Biol Chem 247, 3229, 1972
Rat Rat, Fisher 344, pregnant (19-21 days gestation),
200-250 g		 Hepatocytes Collagenase: 0.3%
 Modified Eagle's w/ Serum Leffert, H., and Paul, D.: Studies on Primary Cultures of Differentiated Fetal Liver Cells, J Cell Biol 52, 559, 1972
Rat Rat, Wistar, male, 260-310 g Hepatocytes Collagenase: 0.01 - 0.08%
 HEPES Seglen, P.: Preparation of Rat Liver Cells, Exp Cell Res 74, 450, 1972
Rat Rat, Fisher, 10 day Epithelial-like Trypsin: 0.25%
 PBS Williams, G., Weisburger, E., and Weisburger, J.: Isolation and Long-Term Cell Culture of Epithelial-Like Cells from Rat Liver, Exp Cell Res 69, 106, 1971
Rat Rat, Wistar, male, 6-8 weeks, 80-160 G Hepatocytes Hyaluronidase: 1.0%
 HBSS, CMF Iype, P.: Cultures from Adult Rat Liver Cells. 1.Establishment of Monolayer Cell-Cultures from Normal Lver, J Cell Physiol 78, 281, 1971
Rat Rat, 100-200 g Hepatocytes Hyaluronidase: 0.10%
 HBSS, CF Haung, Y., and Ebner, K.: Induction of Tyrosine Aminotransferase in Isolated Liver Cells, Biochim Biophys Acta 191, 161, 1969
Rat Rat, SD, adult, 200-300 g Parenchymal Hyaluronidase: 0.10%
Collagenase Type 1: 0.05%
 HBSS, CF Berry, M., and Friend, D.: High Yield Preparation of Isolated Rat Liver Parenchymal Cells, J Cell Biol 43, 506, 1969