Mammary - Tissue Tables
Species | Species Detail | Cells | Enzyme(s) | Medium |
Reference |
Bovine
|
Bovine, lactating Holstein |
Mammary epithelial |
Collagenase Type 3: 400 u/ml
Hyaluronidase: 100 u/ml
Deoxyribonuclease I: 2 u/ml
|
HBSS |
Miranda, S., Wang, Y., Purdie, N., Osborne, V., Coomber, B. and Cant, J.: Selenomethionine Stimulates Expression of Glutathione Peroxidase 1 and 3 and Growth of Bovine Mammary Epithelial Cells in Primary Culture., J Dairy Sci 92,
2670, 2009 |
Bovine
|
Bovine, heifer, 200kg |
Mammary epithelial |
Collagenase Type 2: 1%
Hyaluronidase: 1%
Deoxyribonuclease I: 0.03%
|
M-199 |
Weber M., Purup S., Vestergaard M., Ellis S., Scndergard-Andersen J., Akers R., and Sejrsen K.: Contribution of Insulin-like Growth Factor (IGF)-I and IGF-binding Protein-3 to Mitogenic Activity in Bovine Mammary Extracts and Serum, J Endocrinol 161,
365, 1999 |
Bovine
|
Bovine |
Epithelial |
Hyaluronidase: 0.005%
|
HBSS |
Gibson, C., Vega, J., Baumrucker, C., Oakley, C., and Welsch, C.: Establishment And Characterization Of Bovine Mammary Epithelial Cell Lines, In Vitro Cell Dev Biol 27,
585, 1991 |
Bovine
|
Bovine |
Epithelial |
Deoxyribonuclease I: 0.04%
|
HBSS/Medium 199 |
Baumrucker, C., Deemer, K., Walsh, R., Riss, T., and Akers, R.: Primary Culture of Bovine Mammary Acini on a Collagen Matrix, Tissue Cell
20 (4),
541, 1988 |
Bovine
|
Bovine, young, lactating, female |
Mammary |
Collagenase: 0.30 %
|
HBSS or EBSS |
Anderson, C., and Larson, B.: Comparative Maintenance of Function in Dispersed Cell and Organ Cultures, Exp Cell Res
61,
24, 1970 |
Bovine
|
Bovine, dairy, purebred, (also rat, Holtzmann, albino, white) |
Secretory Mammary gland |
Collagenase: 0.02 - 0.03%
|
HBSS or EBSS |
Schingoethe, D., Hageman, E., and Larson, B.: Essential Amino Acids for Milk Protein Synthesis in the In Vitro Secretory Cell and Stimulation by Elevated Levels, Biochim Biophys Acta 148,
469, 1967 |
Goat
|
Goat, lactating, 1 month |
Mammary gland |
Collagenase: 0.02 - 0.03%
|
HBSS or EBSS |
Blanco, A., Rife, U., and Larson, B.: Lactate Dehydrogenase Isozymes during Dedifferentiation in Cultures of Mammary Secretory Cells, Nature
214,
1331, 1967 |
Guinea-Pig
|
Guinea-pig, pregnant, 4-10 day |
Mammary gland |
Trypsin NF 1:250: 0.25%
|
Dulbecco phosphate |
Turba, F., and Hilpert, N.: Secretion and Resorption of Proteins by Isolated Mammary Gland Cells. German, Biochem Z 334,
501, 1961 |
Human
|
Human |
Mammary epithelial |
Collagenase: 200 u/ml
Hyaluronidase: 100 u/ml
|
DMEM/F-12 |
Labarge, M., Garbe, J. and Stampfer, M.: Processing of Human Reduction Mammoplasty and Mastectomy Tissues for Cell Culture., J Vis Exp
71,
, 2013 |
Human
|
Human, normal biopsy |
Fibroblasts |
Collagenase Type 3: 900 u/ml
|
DMEM/F-12 |
Ronnov-Jessen L., Villadsen R., Edwards J., and Petersen O.: Differential Expression of a Chloride Intracellular Channel Gene, CLIC4, in Transforming Growth Factor-beta1-mediated Conversion of Fibroblasts to Myofibroblasts, Am J Pathol 161,
471, 2002 |
Human
|
Human |
Mammary epithelial cells |
Collagenase Type 1: 0.1%
|
DMEM |
Huss, F. and Kratz, G.: Mammary Epithelial Cell and Adipocyte Co-Culture in a 3-D Matrix: The First Step Towards Tissue-Engineered Human Breast Tissue., Cells Tissues Organs
169,
361-7, 2001 |
Human
|
Human |
Epithelial Fibroblasts |
Collagenase Type 1: 450 IU/ml
|
DMEM/Ham's F-12 |
Ogmundsdottir, H., Petursdottir, I., Gudmundsdottir, I., Amundadottir, L., Ronnov-Jessen, L., and Petersen, O.: Effects of Lymphocytes and Fibroblasts on the Growth of Human Mammary Carcinoma Cells Studied in Short-Term Primary Cultures, In Vitro Cell Dev Biol 29A,
936, 1993 |
Human
|
Human |
Myofibroblasts |
Collagenase: 900 IU/ml
|
DME - F12 |
Ronnov-Jessen, L., VanDeurs, B., Nielsen, M., and Petersen, O.W.: Identification, Paracrine Generation, and Possible Function of Human Breast Carcinoma Myofibroblasts in Culture, In Vitro Cell Dev Biol 28,
273, 1992 |
Human
|
Human, female, 15-61 yrs old |
Epithelial |
Hyaluronidase: 150 IU/ml
|
DMEM |
Berthon, P., Pancino, G., Cremoux, P., Roseto, A., Gespach, C., and Calvo, F.: Characterization of Normal Breast Epithelial Cells in Primary Cultures: Differentiation and Growth Factor Receptors Studies, J Tiss Cul Meth 28A,
716, 1992 |
Human
|
Human |
Epithelial |
Collagenase: 2.0%
|
DMEM/Ham's F-12 |
Emerman, J. and Wilkinson, D.: Routine Culturing of Normal, Dysplastic and Malignant Human Mammary Epithelial Cells from Small Tissue Samples, In Vitro Cell Dev Biol 26,
1186, 1990 |
Human
|
Human |
Tumor, breast |
Neuraminidase: 0.8 u/ml
|
HBSS |
Leung, C., and Shiu, R.: Morphological and Proliferative Characteristics of Human Breast Tumor Cells Cultured on Plastic and in Collagen Matrix, In Vitro 18,
476, 1981 |
Human
|
Human |
Epithelial |
Hyaluronidase: 100 u/ml
|
DMEM/Ham's F-12 |
Stampfer, M., Hallowes, R., and Hackett, A.: Growth of Normal Human Mammary Cells in Culture, In Vitro 16 (5),
415, 1980 |
Mouse
|
Mouse |
Mammary tumor |
Collagenase: 0.15%
Hyaluronidase: 0.020%
|
DMEM/F12 |
Liu, X., Johnson, S., Liu, S., Kanojia, D., Yue, W., Singh, U., Wang, Q, Wang Qi, Nie, Q. and Chen H.: Nonlinear Growth Kinetics of Breast Cancer Stem Cells: Implications for Cancer Stem Cell Targeted Therapy., Sci Rep
3,
2473, 2013 |
Mouse
|
Mouse, 10 week |
Fibroblasts, carcinoma |
Collagenase Type 4: 0.5%
Trypsin: 0.2%
Deoxyribonuclease I: 0.004
Hyaluronidase: 1,000 u/ml
|
DMEM |
Cheng, N. and Lambert, D.: Mammary Transplantation of Stromal Cells and Carcinoma Cells in C57BL/6J Mice., J Vis Exp
54,
e2716, 2011 |
Mouse
|
Mouse, 12 week |
Mammary epithelial |
Collagenase: 0.3%
Hyaluronidase: 100 u/ml
Trypsin: 0.25%
Neutral Protease: 0.5%
Deoxyribonuclease I: 0.01%
|
see reference |
Taddei, I., Deugnier, M., Faraldo, M., Petit, V., Bouvard, D., Medina, D., Fassler, R., Thiery, J. and Glukhova, M.: Beta1 Integrin Deletion from the Basal Compartment of the Mammary Epithelium Affects Stem Cells, Nat Cell Biol 10,
716-22, 2008 |
Mouse
|
Mouse, 11 week |
Epithelial |
Collagenase Type 3: 25 u/ml
Hyaluronidase: 0.1%
Protease XIV: 0.05%
Deoxyribonuclease I: 0.04%
|
DMEM/F12 |
Mueller, S., Clark, J., Myers, P. and Korach, K.: Mammary Gland Development in Adult Mice Requires Epithelial and Stromal Estrogen Receptor Alpha., Endocrinology 143,
2357, 2002 |
Mouse
|
Mouse |
Mammary epithelial stem |
Collagenase Type 3: 0.1%
Hyaluronidase: 0.1%
Pronase: 1.25%
Deoxyribonuclease I: 0.2%
|
DMEM |
Boulanger CA, Smith GH: Reducing mammary cancer risk through premature stem cell senescence, Oncogene
20,
2264-72, 2001 |
Mouse
|
Mouse, BALB/C |
Epithelium |
Collagenase: 250 u/ml
|
HBSS |
Kanazawa, T., and Hosick, H.: Transformed Growth Phenotype of Mouse Mammary Epithelium in Primary Culture Induced by Specific Fetal Mesenchymes, J Cell Physiol 153,
381, 1992 |
Mouse
|
Mouse, BALB/c, virgin, female, 4 month |
Adipocytes |
Trypsin: 50 µg/ml
|
DMEM |
Beck, J., Hosick, H., and Watkins, B.: Growth of Epithelium From a Preneoplastic Mammary Outgrowth on Response to Mammary Adipose Tissue, In Vitro Cell Dev Biol 25 (5),
409, 1989 |
Mouse
|
Mouse (BALB/cCrgl), mature |
Epithelial |
Pronase: 0.01%
|
Hepes buffered medium 199 |
Bandyopadhyay, G., Imagawa, W., Wallace, D., and Nandi, S.: Proliferative effects of insulin and epidermal growth factor on mouse mammary epithelial cells in primary culture, J Biol Chem 263,
7567, 1988 |
Mouse
|
Mouse, BALB/c, female, 6-8 week |
Epithelial |
Collagenase Type 3: 0.1%
|
DMEM |
Ehmann, U., Peterson, W., and Misfeldt, D.: To Grow Mouse Mammary Epithelial Cells in Culture, J Cell Biol
98,
1026, 1984 |
Mouse
|
Mouse (C3H/HeN), female |
Epithelial |
Deoxyribonuclease I: 0.0001%
|
Medium 199 |
Taketani, Y., and Oka, T.: EGF Stimulates Cell Proliferation and Inhibits Functional Differentiation of Mouse Mammalian Dipithelial Cells in Culture, Endocrinology 113,
871, 1983 |
Mouse
|
Mouse, (BALB/c/Crgl Me), female, pregnant |
Epithelial |
Pepsin: 0.1% and 0.05%
Pepsin: 0.1%
|
HBSS with 0.2% EDTA, CMF |
Riser, M., Huff, B., and Medina, D.: Pepsin Can Be Used To Subculture Viable Mammary Epithelial Cells, In Vitro 19,
730, 1983 |
Mouse
|
Mouse, female |
Epithelial |
Deoxyribonuclease I: 0.1%
|
DMEM |
Jones, W., and Hallowes, R.: Isolation of the Epithelial Subcomponents of the Mouse Mammary Gland for Tissue-Level Culture Studies, J Tiss Cul Meth 8 (1),
17, 1983 |
Mouse
|
Mouse (NMuMG), female, 2 months |
Epithelial |
Collagenase Type 3: 0.1%
|
DMEM |
Ehmann, U., and Misfeldt, D.: Mouse Mammary Cells in D-Valine Medium, In Vitro 18,
407, 1982 |
Mouse
|
Mouse, BALB/cCr1, female |
Epithelial |
Collagenase Type 2: 0.2%
|
HBSS/DMEM |
Asch, B., Burstein, N., Vidrich, A., and Sun, T.: Identification of Mouse Mammary Epithelial Cells by Immunofluorescence With Rabbit and Guinea-Pig Antikeratin Antisera, Proc Natl Acad Sci U S A 78,
5643, 1981 |
Mouse
|
Mouse (BALB/cfC3H) |
Mammary tumors Epithelial |
Collagenase: 1.0%
|
HBSS |
Yang, J., Guzman, R., Richards, J., and Nandi, S.: Primary Cultures of Mouse Mammary Tumor Epithelial Cells Embedded in Collagen Gels, In Vitro 16,
502, 1980 |
Mouse
|
Mouse, pregnant 8-12 day |
Mammary |
(see reference):
|
HBSS |
Yang J, Richards J, Guzman R, Imagawa W, and Nandi S.: Sustained growth in primary culture of normal mammary epithelial cells embedded in collagen gels, Proc Natl Acad Sci U S A 77,
2088, 1980 |
Mouse
|
Mouse (BALB/cCrgl), virgin, female, 4-8 month |
Epithelial Mammary gland |
Collagenase Type 3: 0.1%
|
HBSS |
White, M., Hu, A., Hamamoto, S. and Nandi: In vitro analysis of proliferating epithelial cell populations from the mouse mammary gland: fibroblast-free growth and serial passage, In Vitro 14,
271, 1978 |
Mouse
|
Mouse (BALB/c), pregnant, 60-80 days |
Mammary |
Hyaluronidase: 0.1%
|
CMF medium |
Ceriani, R., Peterson, J., and Abraham, S.: Removal of Cell Surface Material by Enzymes Used to Dissociate Mammary Gland Cells, In Vitro 14,
887, 1978 |
Mouse
|
Mouse, BALB/cfC3H/Crgl, virgin, female |
Nodule-transformed |
Hyaluronidase: 0.1%
|
Medium 199 |
DeOme, K., Miyamoto, M., Osborn, R., Guzman, R., and Lum, K.: Detection of Inaparent Nodule-transformed Cells in the Mammary Gland Tissues of Virgn Female BALB/cfC3H Mice, Cancer Res 38,
2103, 1978 |
Mouse
|
Mouse, BALB/cCrgl, female (also guinea-pig) |
Swiss 3T3 |
Collagenase Type 3: 0.2%
|
PBS CMF |
Asch, B., and Medina, D.: Concanavalin A-Induced Agglutinability of Normal, Preneoplastic, and Neoplastic Mouse Mammary Cells, J Natl Cancer Inst 61 (6),
1423, 1978 |
Mouse
|
Mouse, (C3H/Crgl or BALB/cCrgl), 8-10 day pregnant |
Epithelial |
Collagenase Type 3: 0.12%
|
HBSS |
Emerman, J., Enami, J., Pitelka, D., and Nandi, S.: Hormonal Effects on Intracellular and Secreted Casein in Cultures of Mouse Mammary Epithelial Cells on Floating Collagen Membranes, Proc Natl Acad Sci U S A 74 (10),
4466, 1977 |
Mouse
|
Mouse, lactating |
Parenchymal |
Collagenase Type 1: 0.3%
|
Kreb's Ringer bicarbonate buffer |
Kerkof, P., and Abraham, S.: Preparation of Adipose Cell-Free Suspensions of Mammary Gland Parenchymal Cells from Lactating Mice, Methods Enzymol
69,
693, 1976 |
Mouse
|
Mouse, female, early pregnancy <10 days |
Epithelial |
Collagenase: 0.1%
|
Eagle's MEM |
Moore, D., and Lasfargues, E.: Method for the Continuous Cultivation of Mammary Epithelium, In Vitro 7,
21, 1971 |
Mouse
|
Mouse, CBA, virgin, 9/10 wk |
Mammary |
Hyaluronidase: 0.1%
|
BSS, CMF |
Prop, F., and Wiepjes, G.: Improved method for preparation of single-cell suspensions from mammary glands of adult virgin mouse, Exp Cell Res
61,
451, 1970 |
Mouse
|
Mouse, lactating, 14-18 day |
Parenchymal |
Collagenase: 0.33%
|
Kreb's buffer |
Pitelka, D., Kerkof, P., Gagne, H., Smith, S., and Abraham, S.: Characteristics of Cells Dissociated from Mouse Mammary Glands. I. Method of Separation and Morphology of Parenchymal Cells from Lactating Glands, Exp Cell Res
57,
43, 1969 |
Mouse
|
Mouse, lactating, 14 day |
Mammary |
Trypsin NF 1:250: 0.25%
|
HBSS |
Kopelovich, L., Abraham, S., McGrath, H., DeOme, K., Chaikoff, I.: Metabolic Characteristics of a Naturally Ocurring Preneoplastic Tissue. I. Glycolytic Enzyme Activators of Hyperplastic Alveolar Nodule Outgrowths and Adenocarcinomas of Mouse Mammary Gland, Cancer Res 26,
1534, 1966 |
Mouse
|
Mouse, pregnant, 14-17 day |
Mammary |
Collagenase: 0.05% - 0.1%
|
HBSS |
Daniel, C., and DeOme, K.: Growth of Mouse Mammary Glands In Vivo After Monolayer Culture, Science 149,
634, 1965 |
Mouse
|
Mouse, adult, 1-10 days pregnant |
Epithelial Mammary |
Collagenase: 0.02%
|
Simm's |
Lasfargues, E.: Cultivation and Behavior In Vitro of the Normal Mammary Epithelium of the Adult Mouse. II. Observations on the Secretory Activity, Exp Cell Res
13,
553, 1957 |
Rat
|
Rat |
Mammary epithelial |
Collagenase Type 3: 0.35%
|
HBSS |
Mei, N., McDaniel, L., Dobrovolsky, V., Guo, X., Shaddock, J., Mittelstaedt, R., Azuma, M., Shelton, S., McGarrity, L., Doerge, D. and Heflich, R.: The Genotoxicity of Acrylamide and Glycidamide in Big Blue Rats., Toxicol Sci 115,
412, 2010 |
Rat
|
Rat, female, 50 day |
Mammary epithelial |
Collagenase Type 3: 0.15%
|
DMEM/F12 |
Maffini M., Soto A., Calabro J., Ucci A., and Sonnenschein C.: The Stroma as a Crucial Target in Rat Mammary Gland Carcinogenesis, J Cell Sci
117,
1495, 2004 |
Rat
|
Rat, female |
Mammary gland epithelial |
Collagenase Type 3: 0.35%
|
see reference |
Djuric Z., Lewis S., Lu M., Mayhugh M., Naegeli L., Tang N., and Hart R.: Effect of Varying Caloric Restriction Levels on Female Rat Growth and 5-hydroxymethyl-2'-deoxyuridine in DNA, Toxicol Sci 66,
125, 2002 |
Rat
|
Rat, SD |
Mammary fibroblasts |
Collagenase Type 3: 0.2%
Neutral Protease: 0.2%
Deoxyribonuclease I: 0.01%
|
DMEM/F-12 |
Brake P., Zhang L., and Jefcoate C.: Aryl Hydrocarbon Receptor Regulation of Cytochrome P4501B1 in Rat Mammary Fibroblasts: Evidence for Transcriptional Repression by Glucocorticoids, Mol Pharmacol 54,
825, 1998 |
Rat
|
Rat, SD, 50 day |
Mammary epithelial |
Collagenase Type 3: 0.2%
Neutral Protease: 0.2%
|
DMEM/F12 |
Varela L., Darcy K., and Ip M.: The Epidermal Growth Factor Receptor is not Required for Tumor Necrosis Factor-alpha Action in Normal Mammary Epithelial Cells, Endocrinology 138,
3891, 1997 |
Rat
|
Rat (also human) |
Epithelial |
Collagenase Type 1: 0.4%
|
DMEM |
Soriano, J., Pepper, M., Nakamura, T., Orci, L., and Montesano, R.: Hepatocyte Growth Factor Stimulates Extensive Development of Branching Duct-like Structures by Cloned Mammary Gland Epithelial Cells, J Cell Sci
108,
413-430, 1995 |
Rat
|
Rat, SD, female, 60-90 day |
Epithelial |
Collagenase Type 3: 0.5%
|
EBSS |
Laduca, J., and Sinha, D.: In Vitro Carcinogenesis of Mammary Epithelial Cells by N-Nitroso-N-Methylurea Using a Collagen Gel Matrix Culture, In Vitro Cell Dev Biol 29A,
789, 1993 |
Rat
|
Rat, Lewis, female, 90 days |
Epithelial Mammary |
Collagenase: 0.05%
|
Medium 199 |
Lin, T., Hom, Y.K., Richards, J. and Nandi, S.: Effects of Antioxidants and Reduced Oxygen Tension on Rat Mammary Epithelial Cells in Culture, In Vitro Cell Dev Biol 27A,
191, 1991 |
Rat
|
Rat, SD, female, 55 day |
Epithelial |
Neutral Protease: 3 u/ml
|
Medium 199 |
Ehmann, U., Osborn, R., Guzman, R., and Fajardo, L.: Cultured Proliferating Rat Mammary Epithelial Cells, In Vitro Cell Dev Biol 27,
749, 1991 |
Rat
|
Rat, 50-60 days |
Epithelial |
Neutral Protease: 0.2%
|
EBSS |
Hahm, H.A., Ip, M.M.: Primary Culture of Normal Rat Mammary Epithelial Cells Within a Basement Matrix. 1. Regulation of Proliferation by Hormones and Growth Factors, In Vitro Cell Dev Biol 26,
791, 1990 |
Rat
|
Rat, Fisher 344, virgin, 80-100 day |
Epithelial |
Hyaluronidase: 0.1%
|
Medium 199 |
McGrath, M., Palmer, S., and Nandi, S.: Differential Response of Normal Rat Mammary Epithelial Cells to Mammogenic Hormones and EGF, J Cell Physiol 125,
182, 1985 |
Rat
|
Rat, LEW, virgin, female, 45-50 day |
Epithelial |
Collagenase: 0.1%
|
Medium 199 |
Ethler, S.: Primary Culture and Serial Passage of Normal and Carcinogen-Treated Rat Mammary Epithelial Cells In Vitro, J Natl Cancer Inst 74 (6),
1307, 1985 |
Rat
|
Rat, SD, female |
Epithelial, cancer and tumor |
Collagenase: 0.1%
|
Eagles's MEM |
Cohen, L.: Isolation and Characterization of a Serially Cultivated, Neoplastic, Epithelial Cell Line from the N-nitrosomethylurea Induced Rat Mammary Adenocarcinoma, In Vitro 18,
565, 1982 |
Rat
|
Rat, inbred LEW, female, 50-60 day old |
Epithelial |
Collagenase Type 3: 0.1%
|
Medium 199 |
Richards, J., and Nandi, S.: Primary Culture of Rat Mammary Epithelial Cells. I. Effect of Plating Density, Horomones, and Serum on DNA Synthesis, J Natl Cancer Inst 61 (3),
765, 1978 |
Rat
|
Rat, Wistar, 13-18 day postpartum |
Acini |
Collagenase: 0.05%
|
HBSS |
Katz, J., Wals, P. and Van de Velde, R: Lipogenesis by Acini from Mammary Gland of Lactating Rats, J Biol Chem 249,
7348, 1974 |
Rat
|
Rat, SD, 15-20 day postpartum |
Mammary |
Collagenase: 0.2%
|
Kreb's Ringer bicarbonate buffer |
Martin, R., and Baldwin, R.: Effects of Insulin on Isolated Rat Mammary Cell Metabolism: Glucose Utilization and Metabolite Patterns, Endocrinology 89,
1263, 1971 |
Rat
|
Rat, virgin |
Mammary |
Collagenase: 0.35%
|
Medium 199 |
Moon, R., Janns, D., and Young, S.: Preparation of Fat Cell-"Free" Rat Mammary Gland, J Histochem Cytochem 17 (3),
182, 1969 |
|
|