Miscellaneous - Tissue Tables
Species | Species Detail | Cells | Enzyme(s) | Medium |
Reference |
Bovine
|
Bovine |
Nucleus pulposus |
Collagenase Type 2: 0.2%
|
DMEM |
Lee, J., Cheung, K. and Leung, V.: Systematic Study of Cell Isolation from Bovine Nucleus Pulposus: Improving Cell Yield and Experiment Reliability., J Orthop Res 33,
1743-55, 2015 |
Equine
|
Equine |
Tendon Cells |
Collagenase Type 1: 0.1%
|
DMEM |
Nemoto, M., Kizaki, K., Yamamoto, Y., Oonuma, T. and Hashizume, K.: Tenascin-C Expression in Equine Tendon-derived Cells During Proliferation and Migration., J Equine Sci
24,
17-24, 2013 |
Goat
|
Goat |
Infrapatellar fat pad mesenchymal stromal |
Collagenase: 0.15%
|
DMEM |
Arora, A., Sriram, M., Kothari, A. and Katti, D.: Co-culture of Infrapatellar Fat Pad-Derived Mesenchymal Stromal Cells and Articular Chondrocytes in Plasma Clot for Cartilage Tissue Engineering., Cytotherapy
19,
881-894, 2017 |
Human
|
Human |
Adhesion fibroblasts |
Collagenase Type 4: 0.2%
|
Medium 199 |
Foster, D., Marshall, C., Gulati, G., Chinta, M., Nguyen, A., Salhotra, A., Jones, R., Burcham, A., Lerbs, T., Cui, L., King, M., Titan, A., Ransom, R., Manjunath, A., Hu, M. and Blackshear, C.: Elucidating the Fundamental Fibrotic Processes Driving Abdominal Adhesion Formation., Nat Commun
11,
4061, 2020 |
Human
|
Human |
Nucleus pulposus |
Pronase: 0.25%
Collagenase Type 2: 600 u/ml
|
DMEM |
Sun, Y., Lv, M., Zhou, L., Tam, V., Lv, F., Chan, D., Wang, H., Zheng, Z., Cheung, K. and Leung, V.: Enrichment of Committed Human Nucleus Pulposus Cells Expressing Chondroitin Sulfate Proteoglycans under Alginate Encapsulation., Osteoarthritis Cartilage
23,
1194-203, 2015 |
Human
|
Human, 3-7 year |
Dental pulp stem cells |
Collagenase Type 1: 0.3%
Neutral Protease: 0.4%
|
DMEM |
Salmon, B., Bardet, C, Khaddam, M. Naji, J., Coyac, B., Baroukh, B., Letourneur, F., Lesieur, J., Decup, F., Le Denmat, D., Nicoletti, A., Poliard, A, Rowe, P., Huet, E., Vital, S. and Linglart, A.: MEPE-Derived ASARM Peptide Inhibits Odontogenic Differentiation of Dental Pulp Stem Cells and Impairs Mineralization in Tooth Models of X-linked Hypophosphatemia., PLoS ONE
8,
e56749, 2013 |
Human
|
Human |
Dental pulp stem |
Collagenase Type 1: 0.3%
Neutral Protease: 0.4%
|
DMEM/F12 |
Bonnamain, V., Thinard, R., Sergent-Tanguy, S., Huet, P., Bienvenu, G., Naveilhan, P., Farges, J. and Alliot-Licht, B.: Human Dental Pulp Stem Cells Cultured in Serum-Free Supplemented Medium., Front Physiol
4,
357, 2013 |
Human
|
Human |
Mesenchymal stromal |
Collagenase Type 1: 0.075%
|
PBS |
Lee, Y., Lee, J., Park, H., Lim, Y., Lee, J., Wang, S. and Lee, B.: Isolation of Mesenchymal Stromal Cells (MSCs) from Human Adenoid Tissue., Cell Physiol Biochem 31,
513-24, 2013 |
Human
|
Human |
Fungiform taste papillae |
Collagenase Type 1: 550 u/ml
Elastase: 10 u/ml
Soybean Trypsin Inhibitor: 0.09%
|
see reference |
Ozdener, H., Spielman, A. and Rawson, N.: Isolation and Culture of Human Fungiform Taste Papillae Cells, J Vis Exp
63,
e3730, 2012 |
Human
|
Human |
Tendon cells |
Collagenase Type 2: 0,3%
|
DMEM/Hams F12 |
Pauly, S., Klatte, F., Strobel, C., Schmidmaier, G., Greiner, S. and Scheibel, M. Wildemann B: Characterization of Tendon Cell Cultures of the Human Rotator Cuff., Eur Cell Mater
20,
84-97, 2010 |
Human
|
Human |
Adipose derived stromal |
Collagenase Type 2: 0.075%
|
DMEM |
Panetta, N., Gupta, D., Kwan, M., Wan, D., Commons, G. and Longaker, M.: Tissue Harvest by Means of Suction-Assisted or Third-Generation Ultrasound-Assisted Lipoaspiration Has No Effect on Osteogenic Potential of Human Adipose-Derived Stromal Cells., Plast Reconstr Surg
124,
65, 2009 |
Human
|
Human |
Oral epithelial |
Neutral Protease: 1.2 u/ml
Trypsin: 0.05%
|
PBS |
Nakamura, T., Endo, K. and Kinoshita, S.: Identification of Human Oral Keratinocyte Stem/Progenitor Cells by Neurotrophin Receptor p75 and the Role of Neurotrophin/p75 Signaling., Stem Cells 25,
628-38, 2007 |
Human
|
Human |
Carotid artery plaque macrophage |
Collagenase Type 4: 450 u/ml
Deoxyribonuclease I: 500 u/ml
Soybean Trypsin Inhibitor: 0.1%
|
HBSS |
Patino WillmarD, Kang Ju-Gyeong, Matoba Satoaki, Mian OmarY, Gochuico BernadetteR, Hwang PaulM: Atherosclerotic plaque macrophage transcriptional regulators are expressed in blood and modulated by tristetraprolin, Circ Res 98,
1282-9, 2006 |
Human
|
Human |
Esophageal microvascular endothelial |
Collagenase Type 2: 0.2%
|
MCDB-131 |
Rafiee, P., Ogawa, H., Heidemann, J., Li, M., Aslam, M., Lamirand, T., Fisher, P., Graewin, S., Dwinell, M., Johnson, C., Shaker, R. and Binion, D.: Isolation and Characterization of Human Esophageal Microvascular Endothelial Cells: Mechanisms of Inflammatory Activation., Am J Physiol Gastrointest Liver Physiol
285,
G1277, 2003 |
Human
|
|
Gastric epithelial cells |
Collagenase Type 2: 200 u/ml
Neutral Protease: 1.2 u/ml
Soybean Trypsin Inhibitor: 0.125%
|
L-15 |
Smoot, D., Sewchand, J., Young, K., Desbrodes, B., Allen, C. and Naab, T.: A Method for Establishing Primary Cultures of Human Gastric Epithelial Cells, Meth Cell Sci 22,
133, 2000 |
Human
|
Human |
Synoviocytes |
Collagenase: 0.2%
|
DMEM/F12 |
Chen V, Croft D, Purkis P, Kramer IM: Co-culture of synovial fibroblasts and differentiated U937 cells is sufficient for high interleukin-6 but not interleukin-1beta or tumour necrosis factor-alpha release., Br J Rheumatol 37,
148-56, 1998 |
Human
|
Human |
Peptic cells |
Collagenase Type 4: 0.1%
Soybean Trypsin Inhibitor: 0.2%
|
Ringer solution |
Lanas. A., Nerin, J., Esteva, F. and Sainz, R.: Non-Steroidal Anti-Inflammatory Drugs and Prostaglandin Effects on Pepsinogen Secretion by Dispersed Human Peptic Cells., Gut 36,
657-63, 1995 |
Human
|
Human |
Periapical granuloma |
CLSPA: 0.25%
|
RPMI-1640 |
Stern MH, Dreizen S, Mackler BF, Levy BM: Isolation and characterization of inflammatory cells from the human periapical granuloma, J Dent Res 61,
1408-12, 1982 |
Insect
|
Insect, Lepidoptera |
Lepitopteran |
Collagenase Type 3: 0.35%
Hyaluronidase: 0.01%
|
Dulbecco PBS |
Goodwin, R and McCawley, P: Initiating Attached Cell Lines From the Lepidoptera (Insecta), Meth Cell Sci 3,
567, 1977 |
Invertebrate
|
Squid |
Mantle |
Collagenase: see reference
Papain: see reference
|
Phosphate buffer |
Raman, M. and Mathew, S.: Study of Chemical Properties and Evaluation of Collagen in Mantle, Epidermal Connective Tissue and Tentacle of Indian Squid, Loligo duvauceli Orbigny., J Food Sci Technol
51,
1509-16, 2014 |
Mouse
|
Mouse |
Organoid cultures |
Stemxyme 1: 0.1%
|
PBS |
Huang, L., Desai, R., Conrad, D., Leite, N., Akshinthala, D., Lim, C., Gonzalez, R., Muthuswamy, L., Gartner, Z. and Muthuswamy, S.: Commitment and Oncogene-induced Plasticity of Human Stem Cell-derived Pancreatic Acinar and Ductal Organoids.,Cell Stem Cell Vol. 28,,
,
1090-1104.e6, 2021 |
Mouse
|
Mouse, embryonic |
Trachea |
Elastase: 0.1%
Neutral Protease: 0.25%
Deoxyribonuclease I: 0.01%
Trypsin: 0.125%
|
DMEM/F12 |
He, M., Wu, B., Ye, W., Le, D., Sinclair, A., Padovano, V., Chen, Y., Li, K., Sit, R., Tan, M., Caplan, M., Neff, N., Jan, Y., Darmanis, S. and Jan, L.: Chloride Channels Regulate Differentiation and Barrier Functions of the Mammalian Airway., Elife
9,
53085, 2020 |
Mouse
|
Mouse |
Adhesion fibroblasts |
Collagenase Type 4: 0.2%
|
Medium 199 |
Foster, D., Marshall, C., Gulati, G., Chinta, M., Nguyen, A., Salhotra, A., Jones, R., Burcham, A., Lerbs, T., Cui, L., King, M., Titan, A., Ransom, R., Manjunath, A., Hu, M. and Blackshear, C.: Elucidating the Fundamental Fibrotic Processes Driving Abdominal Adhesion Formation., Nat Commun
11,
4061, 2020 |
Mouse
|
Mouse, 8-12 week |
Urinary bladder |
Collagenase Type 1: 50 u/ml
Collagenase Type 3: 40 u/ml
Papain: 45 u/ml
Deoxyribonuclease I: 4 u/ml
Collagenase Type 6: 20 u/ml
|
Tyrode solution |
Atta, H., Gheinani, A., Wacker, A., Heshmati, Y., Bigger-Allen, A., Lambrinos, G., Gao, Y., Bielenberg, D. and Adam, R.: A Single Cell Dissociation Approach for Molecular Analysis of Urinary Bladder in the Mouse Following Spinal Cord Injury., J Vis Exp
,
, 2020 |
Mouse
|
Mouse |
Urothelial |
Collagenase Type 1: 0.5%
|
PBS |
Huang, C., Chen, C. and Shyr, C.: The Anti-Tumor Effect of Intravesical Administration of Normal Urothelial Cells on Bladder Cancer., Cytotherapy
19,
1233-1245, 2017 |
Mouse
|
Mouse |
Bone marrow stromal |
Collagenase Type 1: 0.2%
|
PBS |
Ishida, T., Suzuki, S., Lai, C., Yamazaki, S., Kakuta, S., Iwakura, Y., Nojima, M., Takeuchi, Y., Higashihara, M., Nakauchi, H. and Otsu, M.: Pre-Transplantation Blockade of TNF-α-Mediated Oxygen Species Accumulation Protects Hematopoietic Stem Cells., Stem Cells 35,
989-1002, 2017 |
Mouse
|
Mouse |
Cochlear |
Papain: 20 u/ml
Trypsin: 0.05%
|
DMEM |
Kim, Y., Wang, S., Tymanskyj, S., Ma, L., Tao, H. and Zhang, L.: Dcc Mediates Functional Assembly of Peripheral Auditory Circuits., Sci Rep
6,
23799, 2016 |
Mouse
|
Mouse |
Epithelial stem |
Collagenase: 2%
|
DMEM/F12 |
Chavez, M., Hu, J., Seidel, K., Li, C., Jheon, A., Naveau, A., Horst, O. and Klein, O.: Isolation and Culture of Dental Epithelial Stem Cells from the Adult Mouse Incisor., J Vis Exp
,
, 2014 |
Mouse
|
Mouse, 26-30 g |
Myenteric plexus |
Collagenase Type 2: 0.13%
|
Neurobasal A |
Smith, T., Grider, J., Dewey, W. and Akbarali, H.: Morphine Decreases Enteric Neuron Excitability via Inhibition of Sodium Channels., PLoS ONE
7,
e45251, 2012 |
Mouse
|
Mouse, 10 week |
Synovial mesenchymal |
Collagenase: 0.1%
Deoxyribonuclease I: 0.005%
|
DMEM |
Futami, I., Ishijima, M., Kaneko, H., Tsuji, K., Ichikawa-Tomikawa, N., Sadatsuki, R., Muneta, T., Arikawa-Hirasawa, E., Sekiya, I. and Kaneko, K.: Isolation and Characterization of Multipotential Mesenchymal Cells from the Mouse Synovium., PLoS ONE
7,
e45517, 2012 |
Mouse
|
Mouse |
Spleen, bone marrow endothelial |
Collagenase Type 4: 0.3-1.0%
Deoxyribonuclease I: 20 u/ml
|
PBS |
Shi, C., Jia, T., Mendez-Ferrer, S., Hohl, T., Serbina, N., Lipuma, L., Leiner, I., Li, M., Frenette, P. and Pamer, E.: Bone Marrow Mesenchymal Stem and Progenitor Cells Induce Monocyte Emigration in Response to Circulating Toll-Like Receptor Ligands., Immunity
34,
590, 2011 |
Mouse
|
Mouse |
Dentritic |
Collagenase Type 2: 0.1%
Deoxyribonuclease I: 0.001%
|
DME |
Stock, A., Booth, S. and Cerundolo, V.: Prostaglandin E2 Suppresses the Differentiation of Retinoic Acid-Producing Dendritic Cells in Mice and Humans., J Exp Med 208,
761, 2011 |
Mouse
|
Mouse |
Cochleaer |
Trypsin: 0.125%
|
DMEM/F-12 |
Jan, T., Chai, R., Sayyid, Z. and Cheng, A.: Isolating LacZ-Expressing Cells from Mouse Inner Ear Tissues Using Flow Cytometry., J Vis Exp
58,
e3432, 2011 |
Mouse
|
Mouse, 6-8 week |
Bone marrow mesenchymal stem |
Collagenase Type 1: 0.25%
|
RPMI 1640 |
Xu, S., De Becker, A., Van Camp, B., Vanderkerken, K. and Van Riet, I.: An Improved Harvest and In Vitro Expansion Protocol for Murine Bone Marrow-Derived Mesenchymal Stem Cells., J Biomed Biotechnol
2010,
105940, 2010 |
Mouse
|
Mouse, 3-6 week |
Ear mesenchymal stem |
Collagenase Type 1: 0.2%
|
DMEM/F12 |
Staszkiewicz, J., Gimble, J., Manuel, J. and Gawronska-Kozak, B.: IFATS Collection: Stem Cell Antigen-1-Positive Ear Mesenchymal Stem Cells Display Enhanced Adipogenic Potential., Stem Cells 26,
2666, 2008 |
Mouse
|
Mouse, 6-10 week |
Salivary gland and stomach |
Collagenase Type 4: 0.8%
Deoxyribonuclease I: 1.0%
|
RPMI-1640 |
Ji, H., Rintelen, F., Waltzinger, C., Meier, D, Bilancio, A., Pearce, W., Hirsch, E., Wymann, M., Ruckle, T., Camps, M., Vanhaesebroeck, B., Okkenhaug, K. and Rommel, C.: Inactivation of PI3Kgamma and PI3Kdelta Distorts T-cell Development and Causes Multiple Organ Inflammation., Blood 110,
2940, 2007 |
Mouse
|
Mouse |
Bone marrow |
Collagenase Type 1: 0.15%
Neutral Protease: 0.15%
|
PBS |
Bertoncello, I. and Williams, B.: Hematopoietic Stem Cell Characterization by Hoechst 33342 and Rhodamine 123 Staining., Methods Mol Biol 263,
181, 2004 |
Mouse
|
Mouse, 25-30 g |
Tracheal inflammatory cells |
Collagenase Type 4: 0.1%
Deoxyribonuclease I: 50 u/ml
Soybean Trypsin Inhibitor: 0.1%
|
RPMI 1640 |
Minamoto Kanji, Pinsky DavidJ: Recipient iNOS but not eNOS deficiency reduces luminal narrowing in tracheal allografts, J Exp Med 196,
1321-33, 2002 |
Porcine
|
Porcine, 6 month |
Synoviocytes |
Hyaluronidase: 660 u/ml
Trypsin: 0.25%
Collagenase Type 2: 583 u/ml
|
DMEM |
Kean, T and Dennis, J.: Synoviocyte Derived-Extracellular Matrix Enhances Human Articular Chondrocyte Proliferation and Maintains Re-Differentiation Capacity at Both Low and Atmospheric Oxygen Tensions., PLoS ONE
10,
e0129961, 2015 |
Porcine
|
Porcine, <10 week |
Nucleus pulposus |
Collagenase: 0.05%
Pronase: 0.3%
|
DMEM |
Potier, E. and Ito. K.: Can Notochordal Cells Promote Bone Marrow Stromal Cell Potential for Nucleus Pulposus Enrichment? A Simplified in Vitro System., Tissue Eng Part A
20,
3241-51, 2014 |
Porcine
|
Porcine, various ages |
Nucleous pulposus and AF |
Pronase: 0.05%
Collagenase: 0.2%
|
Modified F-12K |
Cho, H., Park, S., Lee, S., Kang, M., Hasty, K. and Kim, S.: Snapshot of Degenerative Aging of Porcine Intervertebral Disc: A Model to Unravel the Molecular Mechanisms., Exp Mol Med
43,
334-40, 2011 |
Rabbit
|
Rabbit, New Zealand, 8-10 week |
Tenocytes and tendon stem cells |
Collagenase Type 1: 0.3%
Neutral Protease: 0.4%
|
DMEM |
Zhang, J. and Wang, J.: Characterization of Differential Properties of Rabbit Tendon Stem Cells and Tenocytes., BMC Musculoskelet Disord
11,
10, 2010 |
Rat
|
Rat, Lewis, M |
Synovial macrophages |
Collagenase Type 1: 250 u/ml
|
DMEM |
Jain, S., Tran, T. and Amiji, M.: Macrophage Repolarization with Targeted Alginate Nanoparticles Containing IL-10 Plasmid DNA for the Treatment of Experimental Arthritis., Biomaterials 61,
162-77, 2015 |
Rat
|
Rat, SD, 6-8 month |
Pluripotent tendon |
Collagenase Type 1: 0.3%
Neutral Protease: 0.4%
|
DMEM |
Zhou, B., Zhou, Y. and Tang, K.: The Effects of Substance P on Pluripotent Tendon Cells: an In Vitro and In Vivo Study., J Musculoskelet Neuronal Interact
14,
349-58, 2014 |
Rat
|
Rat, Wistar, 7-9 week |
Gingival mitochondria |
Collagenase Type 1: 0.115-0.130 %
|
HBSS |
Kaneko, N., Rikimaru, T., Fujimura, T., Mori, S., Hidaka, S. and Kaya, H.: Preparation of Rat Gingival Mitochondria with an Improved Isolation Method., Int J Dent
2010,
275103, 2010 |
Rat
|
Rat, male, 250-350g |
Submandibular acinar |
Collagenase: 0.05%
|
PBS |
Silver, N., Proctor, GB, Arno, M. and Carpenter GH: Activation of mTOR Coincides with Autophagy During Ligation-induced Atrophy in the Rat Submandibular Gland., Cell Death Dis
1,
e14, 2010 |
Rat
|
Rat, SD, 8-10 week |
Oral fibroblasts |
Collagenase Type 1: 200 u/ml
|
DMEM |
Lin, A., Hokugo, A., Choi, J. and Nishimura, I.: Small Cytoskeleton-Associated Molecule, Fibroblast Growth Factor Receptor 1 Oncogene Partner 2/Wound Inducible Transcript-3.0 (FGFR1OP2/wit3.0), Facilitates Fibroblast-Driven Wound Closure., Am J Pathol 176,
108-21, 2010 |
Rat
|
Rat, 8-10 week |
Synovial cells |
Collagenase Type 1: 250 u/ml
|
RPMI 1640 |
Moghaddami, M., Cleland, L. and Mayrhofer, G.: MHC II+ CD45+ Cells from Synovium-Rich Tissues of Normal Rats: Phenotype, Comparison with Macrophage and Dendritic Cell Lineages and Differentiation into Mature Dendritic Cells In Vitro., Int Immunol 17,
1103, 2005 |
|
|