Pancreas - Tissue Tables
Species | Species Detail | Cells | Enzyme(s) | Medium |
Reference |
Bovine
|
Bovine |
Duct epithelial |
Collagenase: 0.1%
|
HEPES |
Cotton, C., and Al-Nakkash, L.: Isolation and Culture of Bovine Pancreatic Duct Epithelial cells, Am J Physiol 272,
G1328, 1997 |
Bovine
|
Bovine (Bos taurus) |
Ductal |
Neutral Protease: 0.05%
|
EBSS |
Sato, T., Sato, M., Hudson, E., and Jones, R.: Characterization of Bovine Pancreatic Ductal Cells Isolated by a Perfusion-Digestion Technique, In Vitro 19,
651, 1983 |
Bovine
|
Bovine |
Platelets |
Trypsin:
|
(see reference) |
Stiles, G., and Lefkowitz, R.: Hormone-Sensitive Adneylate Cyclase, J Biol Chem 257 (11),
6287, 1982 |
Canine
|
Canine |
Buccal ganglia; SLT muscle |
Trypsin: 0.2%
|
DMEM |
Zoran, M., Doyle, R. and Haydon, P.: Target Contact Regulates the Calcium Responsiveness of the Secretory Machinery During Synaptogenesis, Neuron 6,
145, 1991 |
Canine
|
Canine, adult mongrel, 15-25 kg |
Islets |
Collagenase Type 4: 600-1100 u/ml
Deoxyribonuclease I: 10 ug/ml
|
RPMI 1640 |
Noel, J., Rabinovitch, A., Olson, L., Kyriakides, G., Miller, J., and Mintz, D.: A Method for Large-Scale, High-Yield Isolation of Canine Pancreatic Islets of Langerhans, Metabolism 31 (2),
184, 1982 |
Fish
|
Fish, Osphronemus gourami, 3-month-old, 0.5 mg |
Islets |
Collagenase: 0.12 - 0.46 u/ml
|
RPMI 1640 |
Schrezenmeir, J., Laue, C., Sternheim,E., Wolbert, K., Darquy, S., Chicheportiche, D., Kirchgessner, J., and Reach, G.: Long-Term Function of Single-Cell Preparations of Piscine Principal Islets in Hollow Fibers, Transplant Proc 24 (6),
2941, 1992 |
Guinea-Pig
|
Guinea-pig |
Acinar |
Collagenase Type 3: 60 u/ml
|
Kreb's Ringer |
Schultz, G., Sarras, Jr, M., Gunther, G., Hull, B., Alicea, H., Gorelick, F., and Jamieson, J.: Guinea Pig Pancreatic Acini Prepared with Purified collagenase, Exp Cell Res
130,
49, 1980 |
Guinea-Pig
|
Guinea-pig, Hartley, albino, male, 350-400 g |
Acinar |
Soybean Trypsin Inhibitor: 0.01%
|
Kreb's Ringer |
Gardner, J., Conlon, T., Klaeveman, H., Adams, T., and Ondetti, M.: Action of Cholecystokinin and Cholinergic Agents on Calcium Transport in Isolated Pancreatic Acinar Cells, J Clin Invest 56,
366, 1975 |
Guinea-Pig
|
Guinea-pig |
Exocrine |
Hyaluronidase: 0.15% - 0.2%
|
Kreb's Ringer |
Amsterdam, J., and Jamieson, J.: Structural and Functional Characterization of Isolated Pancreatic Exocrine Cells, Proc Natl Acad Sci U S A 69 (10),
3028, 1972 |
Hamster
|
Hamster |
Islets |
Collagenase Type 4: 1.3% - 2.0%
|
HBSS |
Feldman, J., and Chapman, B.: Preparation of Islets of Langerhans from Rabbits and Hamsters by the Collagenase Digestion Technique, Acta Diabetol 12,
208, 1975 |
Human
|
Human |
Islets |
Collagenase Type 1: 1,600u/isolation
Neutral Protease: 200 u/isolation
Deoxyribonuclease I: 12,000 u/isolation
|
RPMI |
Brissova, M., Haliyur, R., Saunders, D., Shrestha, S., Dai, C., Blodgett, D., Bottino, R., Campbell-Thompson, M., Aramandla, R., Poffenberger, G., Lindner, J., Pan, F., von Herrath, M., Greiner, D. and Shultz, L.: α Cell Function and Gene Expression Are Compromised in Type 1 Diabetes., Cell Rep
22,
2667-2676, 2018 |
Human
|
Human |
Acinar |
CLSPA: 200 u/ml
Soybean Trypsin Inhibitor: 0.01%
|
See Reference |
Cane, M., Sutton, R. and Criddle, D.: Isolation of Human Pancreatic Acinar Cells From Rat and Human Pancreas, Pancreapedia
20,
1, 2011 |
Human
|
Human |
Pancreatic cancer stem cells |
Collagenase Type 4: 200 u/ml
|
medium 199 |
Li, C., Heidt, D., Dalerba, P., Burant, C., Zhang, L., Adsay, V., Wicha, M., Clarke, M. and Simeone, D.: Identification of Pancreatic Cancer Stem Cells., Cancer Res 67,
1030, 2007 |
Human
|
Human, 25-55 yrs old (also porcine) |
Islets |
Collagenase: 0.4%
|
HBSS |
Contractor, H., Johnson, P., Chadwick, D., Robertson, G., and London, N.: The Effect of UW Solution and its Components on the Collagenase Digestion of Human and Porcine Pancreas, Cell Transplant 4,
615, 1995 |
Human
|
Human |
Islets |
Trypsin: Trypsin: 0.125-0.25%
Collagenase: 0.2%
Deoxyribonuclease I: 200 u/ml
|
Eurocollins solution |
Watt, P., Mullen, Y., Benhamou, P., Hober, C., Nomura, Y., Watanabe, Y., Passaro, E., Zinner, M., and Brunicardi, F.: Simplified Semiautomated Method For Isolating Islets From the Human Pancreas, Transplant Proc 26 (2),
582, 1994 |
Human
|
Human, adult organ donors |
Islets |
Collagenase: 0.6%
|
Eurocollins solution |
Warnock, G., Rajotte, R., Evans, M., Ellis, D., DeGroot, T., and Dawidson, I.: Isolation of Islets of Langerhans Following Cold Storage of Human Pancreas, Transplant Proc XIX (4),
3466, 1987 |
Human
|
Human |
Islets |
Collagenase Type 4: 0.8%
|
HBSS |
Izumi, R., Konishi, K., Ueno, K., Shimizu, K., Hirosawa, H., Takahashi, N., and Miyazaki, I.: Isolation of Human Pancreatic Islets from Cryopreserved Pancreas, Transplant Proc XVII,
383, 1985 |
Human
|
Human |
Islets |
Collagenase (1 or 4): 0.60%
|
HBSS |
Gray, D., McShane, P., Grant, A., and Morris, P.: A Method for Isolation of Islets of Langerhans from the Human Pancreas, Diabetes 33,
1055, 1984 |
Human
|
Human, infant, age 1 day-1 year |
Islets |
Collagenase: 170-210 u/ml
|
HBSS |
Sutherland, D., Matas, A., Steffes, M., and Najarian, J.: Infant Human Pancreas: A Potential Source of Islet Tissue for Transplantation, Diabetes 25 (12),
1123, 1976 |
Monkey
|
Monkey, 3-5 Kg |
Islets |
Hyaluronidase: 0.05%
|
HBSS |
Scharp, D., Murphy, J., Newton, W., Ballinger, W., and Lacy, P.: Application of an Improved Isolation Technique for Islet Transplantation in Primates and Rats, Transplant Proc 7,
739, 1975 |
Mouse
|
Mouse |
Islets |
Collagenase Type 4: 0.1%
|
HBSS |
Low, L., Zavortink, M., Mitchell, J., Gan, W., Do, O., Schwiening, C., Gaisano, H. and Thorn, P.: Insulin Secretion from Beta Cells in Intact Mouse Islets is Targeted Towards the Vasculature, Diabetologia 57,
1655, 2014 |
Mouse
|
Mouse |
Islets |
Collagenase: 0.1-0.25%
|
HBSS |
Koh, D., Moody, M. and Jo, J.: Collection of Islets of Langerhans using an Equilibrium Method., Biotechniques
55,
34-7, 2013 |
Mouse
|
Mouse, 8-12 week |
Pancreas organoid |
Collagenase: 0.012%
Neutral Protease: 0.012%
|
DMEM |
Huch, M., Bonfanti, P., Boj, S., Sato, T., Loomans, C., Van de Wetering, M., Sojoodi, M., Li, V., Schuijers, J., Gracanin, A., Ringnalda, F., Begthel, H., Hamer, K., Mulder, J., Van Es, J. and De Koning, E.: Unlimited In Vitro Expansion of Adult Bi-Potent Pancreas Progenitors Through the Lgr5/R-Spondin Axis., EMBO J
32,
2708-21, 2013 |
Mouse
|
Mouse, embryonic |
Pancreatic progenitor |
Neutral Protease: 0.125%
|
DMEM |
Greggio, C., De Franceschi, F., Figueiredo-Larsen, M., Gobaa, S., Ranga, A., Semb, H., Lutolf, M. and Grapin-Botton, A.: Artificial Three-Dimensional Niches Deconstruct Pancreas Development In Vitro., Development
140,
4452-62, 2013 |
Mouse
|
Mouse |
Islets |
Collagenase: 0.2%
|
RPMI 1640 |
Bertera, S., Balamurugan, A., Bottino, R., He, J. and Trucco, M.: Increased Yield and Improved Transplantation Outcome of Mouse Islets with Bovine Serum Albumin., J Transplant
2012,
856386, 2012 |
Mouse
|
Mouse |
Islet |
Collagenase Type 4: 0.2%
|
RPMI-1640 |
Ding, G., Wang, F., Shu, J., Tian, S., Jiang, Y., Zhang, D., Wang, N., Luo, Q., Zhang, Y., Jin, F., Leung, P., Sheng, J. and Huang, H.: Transgenerational Glucose Intolerance with Igf2/H19 Epigenetic Alterations in Mouse Islet Induced by Intrauterine Hyperglycemia., Diabetes 61,
1133-42, 2012 |
Mouse
|
Mouse, 10 week |
Islets |
Collagenase: 0.2%
|
RPMI 1640 |
Kobayashi, T., Yamaguchi, T., Hamanaka, S., Kato-Itoh, M., Yamazaki, Y., Ibata, M., Sato, H., Lee, Y., Usui, J., Knisely, A., Hirabayashi, M. and Nakauchi, H.: Generation of Rat Pancreas in Mouse by Interspecific Blastocyst Injection of Pluripotent Stem Cells., Cell
142,
787, 2010 |
Mouse
|
Mouse, 6-8 month |
Islets |
Collagenase Type 4: 0.1%
|
HBSS |
Taguchi, Y., Tasaki, Y., Terakado, K., Kobayashi, K., Machida, T. and Kobayashi, T.: Impaired Insulin Secretion from the Pancreatic Islets of Hypothyroidal Growth-Retarded Mice., J Endocrinol 206,
195-204, 2010 |
Mouse
|
Mouse, adult, male |
Ancinar |
CLSPA: see reference
Soybean Trypsin Inhibitor: 0.001%
|
DMEM |
Ji, B., Gaiser, S., Chen, X., Ernst, S. and Logsdon, C.: Intracellular Trypsin Induces Pancreatic Acinar Cell Death but not NF-KappaB Activation., J Biol Chem 284,
17488, 2009 |
Mouse
|
Mouse, 3-4 week, 20-24 g |
Islets |
Collagenase Type 4: 0.2%
|
RPMI 1540 |
Huang, H., Xie, Q., Kang, M., Zhang, B., Zhang, H., Chen, J., Zhai, C., Yang, D., Jiang, B. and Wu, Y.: Labeling Transplanted Mice Islet with Polyvinylpyrrolidone Coated Superparamagnetic Iron Oxide Nanoparticles for In Vivo Detection By Magnetic Resonance Imaging., Nanotechnology
20,
365101, 2009 |
Mouse
|
Mouse |
Islets |
Collagenase: 1,000 u/ml
|
RPMI 1640 |
Li, D., Yuan, Y., Tu, H., Liang, Q. and Dai, L.: A Protocol for Islet Isolation from Mouse Pancreas., Nat Protoc
4,
1649, 2009 |
Mouse
|
Mouse |
Islets |
Collagenase: 0.14%
|
RPMI 1640 |
Carter, J., Dula, S., Corbin, K., Wu, R. and Nunemaker, C.: A Practical Guide to Rodent Islet Isolation and Assessment., Biol Proced Online 11,
3, 2009 |
Mouse
|
Mouse |
Islets |
Collagenase: 0.03-0.08%
|
RPMI 1640 |
Szot, G., Koudria, P. and Bluestone, J.: Murine Pancreatic Islet Isolation., J Vis Exp
7,
255, 2007 |
Mouse
|
Mouse, 6-10 month |
Pancreatic ductal |
CLSPA: 50 u/ml
Hyaluronidase: 400 u/ml
Soybean Trypsin Inhibitor: 0.02%
|
DMEM |
Wang, Y., Soyombo, A., Shcheynikov, N., Zeng, W., Dorwart, M., Marino, C., Thomas, P. and Muallem, S.: Slc26a6 Regulates CFTR Activity In Vivo to Determine Pancreatic Duct HCO3- Secretion: Relevance to Cystic Fibrosis., EMBO J
25,
5049, 2006 |
Mouse
|
Mouse, adult |
Acinar |
CLSPA: 200 u/ml
|
see reference |
Voronina, S., Barrow, S., Gerasimenko, O., Petersen, O. and Tepikin, A.: Effects of Secretagogues and Bile Acids on Mitochondrial Membrane Potential of Pancreatic Acinar Cells: Comparison of Different Modes of Evaluating Delta Psi., J Biol Chem 279,
27327, 2004 |
Mouse
|
Mouse, 11-12 week |
Islets |
Collagenase Type 4: 0.2%
|
RPMI |
Astrof, S., Crowley, D., George, E., Fukuda, T., Sekiguchi, K., Hanahan, D. and Hynes, R.: Direct Test of Potential Roles of EIIIA and EIIIB Alternatively Spliced Segments of Fibronectin in Physiological and Tumor Angiogenesis., Mol Cell Biol
24,
8662-70, 2004 |
Mouse
|
Mouse |
Pancreatic islet |
Collagenase Type 4: 0.2%
|
HBSS |
Haefliger Jacques-Antoine, Tawadros Thomas, Meylan Laure, Gurun SabineLe, Roehrich Marc-Estienne, Martin David, Thorens Bernard, Waeber Gerard: The scaffold protein IB1/JIP-1 is a critical mediator of cytokine-induced apoptosis in pancreatic beta cells, J Cell Sci
116,
1463-9, 2003 |
Mouse
|
Mouse |
Islets |
Collagenase Type 4: 0.2%
|
HBSS |
Wu Yulian, Han Bing, Luo Hongyu, Roduit Raphael, Salcedo TheodoraW, Moore PaulA, Zhang Jun, Wu Jiangping: DcR3/TR6 effectively prevents islet primary nonfunction after transplantation, Diabetes 52,
2279-86, 2003 |
Mouse
|
Mouse, BALB/c, 6-8 week-old, either sex |
Acinar |
Collagenase: 0.1%
|
Waymouth's MB |
Kurup, S., and Bhonde, R.: Analysis and Optimization of Nutritional Set-up for Murine Pancreatic Acinar Cells, JOP
3 (1),
8, 2002 |
Mouse
|
Mouse |
Islets |
Collagenase Type 2: 0.2%
|
CF Medium |
Koster, J., Marshall, B., Ensor, N., Corbett, J., and Nichols, C.: Targeted Overativity of Cell KATP Channels Induces Profound Neonatal Diabetes, Cell
100,
645, 2000 |
Mouse
|
Mouse |
Pancreatic islets |
Collagenase Type 4: 0.4%
|
Gey's BSS |
Strowski M, Parmar R, Blake A, Schaeffer J: Somatostatin inhibits insulin and glucagon secretion via two receptors subtypes: an in vitro study of pancreatic islets from somatostatin receptor 2 knockout mice, Endocrinology 141,
111-7, 2000 |
Mouse
|
Mouse |
Acinar cells and acini |
Collagenase Type 1: see reference
CLSPA: see reference
|
see reference |
Toivola, D., Ku, N., Ghori, N., Lowe, A., Michie, S. and Omary, M.: Effects of Keratin Filament Disruption on Exocrine Pancreas-Stimulated Secretion and Susceptibility to Injury., Exp Cell Res
255,
156, 2000 |
Mouse
|
Mouse, albino, 25 g |
Acinar |
CLSPA: see reference
|
see reference |
Fogarty, K., Kidd, J., Tuft, R. and Thorn, P.: A Bimodal Pattern of InsP(3)-Evoked Elementary Ca(2+) Signals in Pancreatic Acinar Cells., Biophys J
78,
2298, 2000 |
Mouse
|
Mouse |
Duct |
Papain: 25 u/ml
|
DMEM /F-12 |
Githens, S: Pancreatic Duct Epithelial Cells,Cell & Tissue Culture: Laboratory Procedures Vol. 1,Doyle, A., Griffiths, J., and Newell, D.,
John Wiley and Sons, Ltd.,
12B:12.1, 1995 |
Mouse
|
Mouse |
Acinar |
Collagenase: 100 u/ml
|
HEPES |
Jauch, P., Peterson, O., and Lauger, P.: Electrogenic Properties of the Na-Alanine Cotransporter in Acinar Cells, J Membr Biol 94,
99, 1986 |
Mouse
|
Mouse (C57BL/6J-ob/ob), 9-12 wks, male |
Islets |
Hyaluronidase: 0.5%
|
Kreb's Ringer bicarbonate buffer |
Dalpe-Scott, M., Heick, H., and Begin-Heick, N.: Secretion in the Obese (ob/ob) Mouse. The Effect of Oxytetracycline on Insulin Release, Diabetes 32,
932, 1983 |
Mouse
|
Mouse, male, 18-24 g |
Acinar |
CLSPA: 70-90 u/ml
Soybean Trypsin Inhibitor: 0.01%
|
Krebs-Henseleit |
Burnham DB, Williams JA: Effects of carbachol, cholecystokinin, and insulin on protein phosphorylation in isolated pancreatic acini, J Biol Chem 257,
10523-8, 1982 |
Mouse
|
Mouse, 7-10 week |
Islets |
Collagenase: see reference
|
DMEM |
Yesil, P, Michel, M., Chwalek, K., Pedack, S., Jany, C., Ludwig, B., Bornstein, S. and Lammert, E.: A New Collagenase Blend Increases the Number of Islets Isolated from Mouse Pancreas., Islets
1,
185, |
Porcine
|
Porcine, juvenile |
Islets |
Collagenase: see reference
|
Univ of Wisconsin solution |
Van der Burg Michael P M, Graham John M: Iodixanol Density Gradient Preparation in University of Wisconsin solution for porcine islet purification, ScientificWorldJournal
3,
1154-9, 2003 |
Porcine
|
Porcine, 3 month, 15-20 kg |
Acinar |
Collagenase Type 3: 200 u/ml
|
RPMI 1640 |
Zhao, X., Han, J., and Tang, C.: Primary Culture of Porcine Pancreatic Acinar Cells, JOP
2 (2),
78, 2001 |
Porcine
|
Porcine, 1-3 year, 1.5-2.0 kg, either sex |
Islets |
Collagenase: 0.25%
|
HBSS |
Korbutt, G., Elliott, J., Ao, Z., Smith, D., Warnock, G., and Rajotte, R.: Large Scale Isolation, Growth, and Function of Porcine Neonatal Islet Cells, J Clin Invest 97 (9),
2119, 1996 |
Porcine
|
Porcine, female |
Islets |
Collagenase: 0.1%
|
HBSS |
Brandhorst, D., Brandhorst, H., Hering, B., Federlin, K., and Bretzel, R.: Islet Isolation from the Pancreas of Large Mammals and Humans: 10 Years of Experience, Exp Clin Endocrinol 103,
3, 1995 |
Porcine
|
Porcine |
Islets |
Collagenase: 0.1%-0.2%
|
HBSS |
Johnson, P., van Suylichem, P., Roberts, D., Vos-Scheperkeuter, G., White, S., van Schlifgaarde, R., London, N.: Design of a Simple, in vitro Method for Evaluation of the Efficiency of Crude Clostridium histolyticum Collagenase and its Components for Porcine Islet Isolation, Xenotransplantation 2,
165, 1995 |
Porcine
|
Porcine, <2 year, 200-250 kg |
Islets |
Collagenase: 0.1%
|
HBSS |
Heiser, A., Ulrichs, K., and Muller-Ruchholtz, W.: Isolation of Porcine Pancreatic Islets: Low Trypsin Activity During the Isolation Procedure Guarantees Reproducible High Islet Yields, J Clin Lab Anal 8,
407, 1994 |
Porcine
|
Porcine, 10-36 months, 200-300 kg |
Islets |
Collagenase: 0.2%
|
HBSS |
Ricordi, C., Socci, C., Davalli, A., Staudacher, C., Baro, P., Vertova, A., Sassi, I., Gavazzi, F., Pozza, G., and Di Carlo, V.: Isolation of the Elusive Pig Islet, Surgery 107 (6),
688, 1990 |
Porcine
|
Porcine |
Acinar |
Collagenase: 100 u/ml
|
Saline |
Iwatsuki, N., and Peterson, O.: Action of Tetraethylammonium on Calcium-Activated Potassim Channels in Pig Pancreatic Acinar Cells Studied by Patch-Clamp Single-Channel and Whole-Cell Current Recording, J Membr Biol 86,
139, 1985 |
Rabbit
|
Rabbit, New Zealand white, Male/Female, 2-3 Kg |
Acinar |
Hyaluronidase: 0.2%
|
Kreb's Ringer bicarbonate buffer |
Renckens, B., Schrijen, J., Swarts, H., DePont, J., and Bonting, S.: Role of Calcium in Exocrine Pancreatic Secretion. IV. Calcium Movements in Isolated Acinar Cells of Rabbit Pancreas, Biochim Biophys Acta 544,
338, 1978 |
Rabbit
|
Rabbit, also hamster |
Islets |
Collagenase Type 4: 1.3% - 2.0%
|
HBSS |
Feldman, J., and Chapman, B.: Preparation of Islets of Langerhans from Rabbits and Hamsters by the Collagenase Digestion Technique, Acta Diabetol 12,
208, 1975 |
Rat
|
Rat, SD, adult |
Acinar |
Collagenase: 0.01%
Soybean Trypsin Inhibitor: 0.001%
|
Culture media |
Li, J., Zhou, R., Bie, B., Huang, N., Guo, Y., Chen, H., Shi, M., Yang, J., Zhang, J. and Li, Z.: Emodin and Baicalein Inhibit Sodium Taurocholate-Induced Vacuole Formation in Pancreatic Acinar Cells., World J Gastroenterol
24,
35-45, 2018 |
Rat
|
Rat, Wistar, 300g |
Islets |
Collagenase Type 4: 0.2%
|
CMRL 1066 |
Verga Falzacappa, C., Mangialardo, C., Raffa, S., Mancuso, A., Piergrossi, P., Moriggi, G., Piro, S., Stigliano, A., Torrisi, M., Brunetti, E., Toscano, V. and Misiti, S.: The Thyroid Hormone T3 Improves Function and Survival of Rat Pancreatic Islets During In Vitro Culture., Islets
2,
96, 2010 |
Rat
|
Rat |
Acinar |
CLSPA: 100 u/ml
|
DMEM |
Williams, J.: Isolation of Rodent Pancreatic Acinar Cells and Acini by Collagenase Digestion, Pancreapedia
,
, 2010 |
Rat
|
Rat, adult |
Islets |
Collagenase Type 1: 450 u/ml
|
Leibowitz L-15 |
Williams, J., Huang, H., Kover, K., Moore, W., Berkland, C., Singh, M., Smirnova, I., MacGregor, R. and Stehno-Bittel, L.: Reduction of Diffusion Barriers in Isolated Rat Islets Improves Survival, but not Insulin Secretion or Transplantation Outcome., Organogenesis 6,
115, 2010 |
Rat
|
Rat, male, 7-11 week |
Islets |
Collagenase Type 4: see reference
|
RPMI 1640 |
Getty-Kaushik, L., Richard, A., Deeney, J., Shirihai, O. and Corkey, B.: The CB1 Antagonist Rimonabant Decreases Insulin Hypersecretion in Rat Pancreatic Islets, Obesity
17,
1856, 2009 |
Rat
|
Rat |
Islets |
Collagenase Type 1: 450 u/ml
|
RPMI 1640 |
MacGregor, R., Williams, S., Tong, P., Kover, K., Moore, W. and Stehno-Bittel, L.: Small Rat Islets are Superior to Large Islets in In Vitro Function and in Transplantation Outcomes., Am J Physiol/Endo 290,
E771, 2006 |
Rat
|
Rat, Wistar, male, 250-400g |
Pancreatic islets |
Collagenase: 0.75%
|
RPMI 1640 |
Tian XH, Xue WJ, Ding XM, Pang XL, Teng Y, Tian PX, and Feng XS: Small intestinal submucosa improves islet survival and function during in vitro culture, World J Gastroenterol
11,
7378, 2005 |
Rat
|
Rat, 250-350 g |
Pancreatic islet |
Collagenase Type 4: 0.2%
|
HBSS |
Haefliger Jacques-Antoine, Tawadros Thomas, Meylan Laure, Gurun SabineLe, Roehrich Marc-Estienne, Martin David, Thorens Bernard, Waeber Gerard: The scaffold protein IB1/JIP-1 is a critical mediator of cytokine-induced apoptosis in pancreatic beta cells, J Cell Sci
116,
1463-9, 2003 |
Rat
|
Rat |
Pancreatic acini |
CLSPA: 30 u/ml
Collagenase Type 4: 30 u/ml
Soybean Trypsin Inhibitor: 0.01%
|
M199 |
Blinman TA, Gukovsky I, Mouria M, Zaninovic V, Livingston E, Pandol SJ, Gukovskaya AS: Activation of pancreatic acinar cells on isolation from tissue: cytokine upregulation via p38 MAP kinase, Am J Physiol Cell Physiol
279,
C1993-2003, 2000 |
Rat
|
Rat, SD, male |
Ancinar |
CLSPA: see reference
Soybean Trypsin Inhibitor: 0.01%
|
DMEM |
Ji B, Kopin AS, Logsdon CD: Species differences between rat and mouse CCKA receptors determine the divergent acinar cell response to the cholecystokinin analog JMV-180, J Biol Chem 275,
19115-20, 2000 |
Rat
|
Rat |
Islets |
Collagenase: 126 - 196 u/ml
|
HBSS |
Verspohl, E., and Wienecke, A.: The Role of Protein Kinase C in the Desensitization of Rat Pancreatic Islets to Chrolinergic stimulation, J Endocrinol 159,
287, 1998 |
Rat
|
Rat |
Islets |
Collagenase: 0.5 - 0.9%
|
HBSS |
Takaki, R and Ono J: Culture of Pancreatic Islet Cells,Cell & Tissue Culture: Laboratory Procedures Vol. 1,Doyle, A., Griffiths, J., and Newell, D.,
John Wiley and Sons, Ltd.,
12B:11.1, 1995 |
Rat
|
Rat |
Duct |
Papain: 25 u/ml
|
DMEM /F-12 |
Githens, S: Pancreatic Duct Epithelial Cells,Cell & Tissue Culture: Laboratory Procedures Vol. 1,Doyle, A., Griffiths, J., and Newell, D.,
John Wiley and Sons, Ltd.,
12B:12.1, 1995 |
Rat
|
Rat, SD, male, 40 - 100 g |
Acinar |
Hyaluronidase: 462 u/ml
|
Ham's F12 |
Hirschi, K., Kenny, S., Justice, J., Brannon, P.: Effects of Secretin And Caerulein On Enzymes Of Cultured Pancreatic Acinar Cells, In Vitro Cell Dev Biol 27,
660, 1991 |
Rat
|
Rat, SD, male, 100 - 150 g |
Parotid acinar |
Trypsin: 0.001%
|
F12 medium |
Yeh, C., Mertz, P., Oliver, C., Baum, B., and Kousvelari, E.: Cellular Characteristics of Long-Term Cultured Rat Parotid Acinar Cells, In Vitro Cell Dev Biol 27,
707, 1991 |
Rat
|
Rat, Wistar, male |
Parotid acinar |
Trypsin: 0.02%
|
Solution B (See reference) |
Foskett, J., Roifman, C. and Wong, D.: Activation of Calcium Oscillations by Thapsigargin in Parotid Acinar Cells, J Biol Chem 266,
2778, 1991 |
Rat
|
Rat, S-Wistar, male, 230 - 270 g (also mouse, 6 - 8 wk old) |
Islets |
Collagenase: 0.1% - 0.2%
|
HBSS |
Ohzato, H., Gotoh, M., Monden, M., Dono, K., Kanai, T., and Mori, T.: Improvement in the Islet Yield From a Cold-Preserved Pancreas by Pancreatic Ductal Collagenase Distention at the Time of Harvesting, Transplantation 51,
566, 1991 |
Rat
|
Rat, SD, both sexes, 6-20 wks old |
Interlobular ducts |
Papain: 25 u/ml
|
DMEM/Ham's F-12 |
Githens, S., Schexnayder, J., Desai, K., and Patke, C.: Rat Pancreatic Interlobular Duct Epithelium: Isolation and Culture in Collagen Gel, In Vitro Cell Dev Biol 25 (8),
679, 1989 |
Rat
|
Rat, SD, male, 150 - 200 g |
Acinar |
Soybean Trypsin Inhibitor: 0.01%
|
HEPES |
Menozzi, D., Sato, S., Jensen, R., and Gardner, J.: Cyclic GMP Does Not Inhibit Protein Kinase C-Mediated Enzyme Secretion in Rat Pancreatic Acini, J Biol Chem 264,
995, 1989 |
Rat
|
Rat, Wistar, male, 250 - 350 g |
Acinar, parotid |
Hyaluronidase: 0.015%
|
Earle's MEM |
Melvin, J., Kawaguchi, M., Baum, B., and Turner, R.: A Muscarinic Agonist-Stimulated Chloride Efflux Pathway is Associated With Fluid Secretion in Rat Parotid Acinar Cells, Biochem Biophys Res Commun
145,
754, 1987 |
Rat
|
Rat, SD, male, 50 - 125 g |
Acinar Exorbital lacrimal, parotid, pancreas |
Trypsin: 0.01%
|
HBSS, CMF |
Oliver, C., Waters, J., Tolbert, C., and Kleinman, H.: Growth of Exocrine Acinar Cells on a Reconstituted Basement Membrane Gel, In Vitro Cell Dev Biol 23,
465, 1987 |
Rat
|
Rat, Fischer-344, either sex, 120-150 g |
Epithelial |
Trypsin: 0.1%
|
Ham's F-12/HBSS (see reference) |
Tsao, M., and Duguid, W.: Establishment of Propagable Epithelial Cell Lines From Normal Adult Rat Pancreas, Exp Cell Res
168,
365, 1987 |
Rat
|
Rat, 200-350 g |
Islets |
Collagenase Type 4: 0.2%
|
HBSS |
Gotoh, M., Maki, T., Satomi, S., Porter, J., Bonner-Weir, S., O'Hara, C., and Monaco, A.: Reproducible High Yield of Rat Islets By Stationary In Vitro Digestion Following Pancreatic Ductal or Portal Venous Collagenase Injection, Transplantation 43 (5),
725, 1987 |
Rat
|
Rat, SD, male, 42 - 48 day, 175 - 200 g |
Acinar, submandibular gland |
Hyaluronidase: 0.1 %
|
HBSS, CF |
Quissell, D., Redman, R., and Mark, M.: Short-Term Primary Culture of Acinar-Intercalated Duct Complexes From Rat Submandibular Glands, In Vitro Cell Dev Biol 22,
469, 1986 |
Rat
|
Rat, Dark Agouti and Lewis rat donors, adult |
Islets |
Collagenase Type 1: 0.3%
|
Hank's solution |
Sutton, R., Peters, M., McShane, P., Gray, D., and Morris, P.: An Improved Method for the Isolation of Islets of Langerhans From the Adult Rat Pancreas, Transplant Proc XVII (6),
1819, 1986 |
Rat
|
Rat, SD, male, 50-75 g |
Acinar |
Hyaluronidase: 0.1%
|
HBSS (see reference) |
Brannon, P., Orrison, B., and Kretchmer, N.: Primary Cultures of Rat Pancreatic Acinar Cells in Serum-Free Medium, In Vitro Cell Dev Biol 21 (1),
6, 1985 |
Rat
|
Rat, SD, 125 - 350 g |
Duct |
Trypsin: 0.01%
|
HBSS |
Githens, S., Holmquist, D., Whelan, J., and Ruby, J.: Ducts of the Rat Pancreas in Agarose Matrix Culture, In Vitro 16,
797, 1980 |
Rat
|
Rat |
Exocrine |
Hyaluronidase: 0.9%
|
Kreb's Ringer |
Schulz, I., Heil, K., Kribben, A., Sachs, G., and Haase, W.: Isolation and Functional Characterization of Cells From the Exocrine Pancreas,Biology of Normal and Cancerous Exocrine Pancreatic Cells,Ribet, A., Pradayrol, L., and Susini, C.,
Elsevier,
, 1980 |
Rat
|
Rat, Wistar, male |
Islets |
Collagenase Type 4: 1%
|
Medium 199 |
Katada, T., and Ui, M.: Enhanced Insulin Secretion and Cyclic Amp Accumulation in Pancreatic Islets Due to Activation of Native Calcium Ionophores, J Biol Chem 254 (2),
469, 1979 |
Rat
|
Rat, SD, male, 250 - 350 g (also mouse, white, Swiss, male, 20 - 24 g) |
Acinar |
Hyaluronidase: 0.18%
|
Kreb's Henseleit bicarbonate buffer |
Williams, J., Korc, M., and Dormer, R.: Action of Secretagogues on a New Preparation of Functionally Intact, Isolated Pancreatic Acini, Am J Physiol 235,
517, 1978 |
Rat
|
Rat, Wistar, male, 300-350 g |
Islets |
Collagenase: 0.2%
|
Kreb's Ringer bicarbonate buffer |
Wolters, G., Konijenendijk, W., and Bouman, P.: Effects of Fasting on Insulin Secretion, Islet Glucose Metabolism, and the Cyclic Adenosine 3'5'-Monophosphate Content of Rat Pancreatic Islets In Vitro, Diabetes 26 (6),
530, 1977 |
Rat
|
Rat, Wistar, male, 200 - 300 g |
Islets |
Collagenase Type 4: 0.5%
|
HBSS |
Shibata, A., Ludvigsen, C., Naber, S., McKaneil M., and Lacy, P.: Standardization of a Digestion-Filtration Method for Isolation of Islets, Diabetes 25,
667, 1976 |
Rat
|
Rat |
Exocrine |
Hyaluronidase: 0.15%
|
Krebs |
Kondo, S., and Schulz, I.: Calcium Ion Uptake in Isolated Pancreas Cells Induced by Secretagogues, Biochim Biophys Acta 419,
76, 1976 |
Rat
|
Rat, Wistar-Lewis, fetal 18-20 day gestation (also neonatal 3-12 day old) |
Islets |
Collagenase Type 4: 0.63%
|
EBSS (see reference) |
Braaten, J., Jarlfors, U., Smith, D., and Mintz, D.: Purification of Monolayer Cell Cultures of the Endocrine Pancreas, Tissue Cell
7 (4),
747, 1975 |
Rat
|
Rat, SD, albino, male, 150-200 g |
Acinar, parotid |
Trypsin: 0.01%
Collagenase: 40-50 u/ml
Hyaluronidase: 0.10%
|
HBSS CMF |
Mangos, J., McSherry, N., Butcher, F., Irwin, K., and Barber, T.: Dispersed Rat Parotid Acinar Cells (Morphological, Functional Character), Am J Physiol 229 (3),
560, 1975 |
Rat
|
Rat, neonate |
Islets |
Trypsin: 0.05%
|
Puck's saline buffered w/ EDTA 0.02% |
Leonard, R., Lazarow, A., and Hegre, O.: Pancreatic Islet Transplantation in the Rat, Diabetes 22,
413, 1973 |
Rat
|
Rat, male, albino, 200-300 g |
Islets |
Collagenase: 0.5%
|
Hanks solution |
Lacy, P., Walker, M., and Fink, J.: Perifusion of Isolated Rat Islets in Vitro, Diabetes 21 (10),
987, 1972 |
Rat
|
Rat, Lewis, inbred |
Islets |
Collagenase: 0.5%
|
HBSS |
Ballinger, W., and Lacy, P.: Transplantation of Intact Pancreatic Islets in Rats, Surgery 72 (2),
175, 1972 |
Rat
|
Rat, Wistar, albino, male, 400 - 500 g |
Islets |
Collagenase Type 4: 1.0% - 1.2%
|
HBSS |
Lacy, P., and Kostianovsky, M.: Method for the Isolation of Intact Islets of Langerhans from the Rat Pancreas, Diabetes 16,
35, 1967 |
Rat
|
Rat |
Ascites hepatoma |
Trypsin: 0.1%
|
Phosphate buffer (see reference) |
Essner, E.: Experiments on an Ascites Hepatoma. I. Enzymatic Digestion and Alkaline Degradation of the Cementing Substance and Separation of Cells, in Tumor Islands, Exp Cell Res
7,
430, 1954 |
|
|