Parotid - Tissue Tables

SpeciesSpecies DetailCellsEnzyme(s)Medium Reference
Mouse Mouse Parotid and sublingual glandular Neutral Protease: 50 u/ml
Collagenase Type 1: 100 u/ml
 PBS Ogawa, M., Oshima, M., Imamura, A., Sekine, Y., Ishida, K., Yamashita, K., Nakajima, K., Hirayama, M., Tachikawa, T. and Tsuji, T.: Functional Salivary Gland Regeneration by Transplantation of a Bioengineered Organ Germ., Nat Commun 4, 2498, 2013
Rat Rat, Wistar, male, 130 g Acinar Collagenase: 75 u/ml
Hyaluronidase: 153 u/ml
 RPMI 1640 Looms, D., Dissing, S., Tritsaris, K., Pedersen, A. and Nauntofte, B.: Adrenoceptor-Activated Nitric Oxide Synthesis in Salivary Acinar Cells., Adv Dent Res 14, 62, 2000
Rat Rat, SD, 100-120 g Parotid acinar cells Collagenase Type 2: 90 u/ml
 Krebs-Henseleit Bicarbonate D'Silva NJ, DiJulio DH, Belton CM, Jacobson KL, Watson EL: Immunolocalization of Rap1 in the rat parotid gland: detection on secretory granule membranes, J Histochem Cytochem 45, 965-73, 1997
Rat Rat, SD, male Epithelial Collagenase: 50-75 u/ml
Hyaluronidase: 0.1%
 HBSS CF Prasad, K.N., Edwards-Prasad, J., Carvaiho, I., LaRosa, F.G., Baibinder, E., Meyers, A., and Quissell, D.: Establishment of Primary Cultures of Rat and Human Parotid Epithelial Cells for Transfection Experiments, In Vitro Cell Dev Biol 28, 493, 1992
Rat Rat, SD, male, 100-150 g Parotid acinar Trypsin: 0.001%
 F12 medium Yeh, C., Mertz, P., Oliver, C., Baum, B., and Kousvelari, E.: Cellular Characteristics of Long-Term Cultured Rat Parotid Acinar Cells, In Vitro Cell Dev Biol 27, 707, 1991
Rat Rat, Wistar, male Parotid acinar Trypsin: 0.02%
 Solution B
(See reference)		 Foskett, J., Roifman, C. and Wong, D.: Activation of Calcium Oscillations by Thapsigargin in Parotid Acinar Cells, J Biol Chem 266, 2778, 1991
Rat Rat Parotid Hyaluronidase: 0.025%
 HBSS with 20mM HEPES Takuma, T. and Ichida, T.: Amylase Secretion From Saponin-Permeabilized Parotid Cells Evoked by Cyclic AMP, J Invest Dermatol 103, 95, 1988
Rat Rat, Wistar, male, 250 - 350 g Acinar, parotid Hyaluronidase: 0.015%
 Earle's MEM Melvin, J., Kawaguchi, M., Baum, B., and Turner, R.: A Muscarinic Agonist-Stimulated Chloride Efflux Pathway is Associated With Fluid Secretion in Rat Parotid Acinar Cells, Biochem Biophys Res Commun 145, 754, 1987
Rat Rat, SD, male, 50 - 125 g Acinar
Exorbital lacrimal, parotid, pancreas		 Trypsin: 0.01%
 HBSS, CMF Oliver, C., Waters, J., Tolbert, C., and Kleinman, H.: Growth of Exocrine Acinar Cells on a Reconstituted Basement Membrane Gel, In Vitro Cell Dev Biol 23, 465, 1987
Rat Rat, SD, albino, male, 150-200 g Acinar, parotid Trypsin: 0.01%
Collagenase: 40-50 u/ml
Hyaluronidase: 0.10%
 HBSS CMF Mangos, J., McSherry, N., Butcher, F., Irwin, K., and Barber, T.: Dispersed Rat Parotid Acinar Cells (Morphological, Functional Character), Am J Physiol 229 (3), 560, 1975