Reproductive - Tissue Tables
Species | Species Detail | Cells | Enzyme(s) | Medium |
Reference |
Bovine
|
Bovine |
Corpus leuteal cells |
Collagenase Type 4: 420 u/ml
|
M-199 |
Levy N, Gordin M, Mamluk R, Yanagisawa M, Smith M F, Hampton J H, Meidan R: Distinct cellular localization and regulation of endothelin-1 and endothelin-converting enzyme-1 expression in the bovine corpus luteum: implications for luteolysis, Endocrinology 142,
5254-60, 2001 |
Bovine
|
Bovine, female |
Leuteal |
Collagenase Type 1: 0.2%
|
Ham's F-12 |
Tsang PC, Poff JP, Boulton EP, Condon WA: Four-day-old bovine corpus luteum: progesterone production and identification of matrix metalloproteinase activity in vitro, Biol Reprod 53,
1160-8, 1995 |
Bovine
|
Calf, mid to late gestational |
Fibroblasts |
Collagenase: 0.1%
|
Medium 199 |
Coplen, D., Howard, P., Duckett, J., Snyder, H., and Macarak, E.: Characterization of a Fibroblast Cell From the Urinary Bladder Wall, In Vitro Cell Dev Biol 30A,
604, 1994 |
Bovine
|
Bovine |
Epithelial Endometrial |
Collagenase Type 2: 0.1%
|
DMEM/EBSS |
Munson, L., Chandler, S., and Schlafer, D.: Long-Term Culture of Bovine Trophoblastic Cells, J Tiss Cul Meth 11 (3),
123, 1988 |
Bovine
|
Bovine (also porcine, human) |
Interna & corpus luteum Endometrium Ovarian Uterine |
Pronase: 0.1%
|
Moscona's BSS |
Marcus, G., Connor, L., Domingo, M., Tsang, B., Downey, B., and Ainsworth, L.: Enzymatic Dissociation of Ovarian and Uterine Tissues, Endocr Res 10,
151, 1984 |
Canine
|
Canine |
Amniotic membrane MSC |
Trypsin: 0.25%
Collagenase Type 1: 0.2%
|
LG-DMEM |
Park, S., Seo, M., Kim, H. and Kang, K.: Isolation and Characterization of Canine Amniotic Membrane-Derived Multipotent Stem Cells., PLoS ONE
7,
e44693, 2012 |
Chicken
|
Chicken, Gallus Domesticus,20-30 week |
Primary follicles |
Trypsin: 0.15%
Collagenase Type 1: 0.125%
|
Dulbecco's phosphate buffered saline |
Du Meihong, Han Haitang, Jiang Bin, Zhao Chen, Qian Changsong, Shen Haiyan, Xu Yan, Li Zandong: An efficient isolation method for domestic hen (Gallus domesticus) ovarian primary follicles, J Reprod Dev
52,
569-76, 2006 |
Fish
|
Sebastes Schlegel |
Ovarian |
Collagenase Type 1: 500 u/ml
|
L-15 |
Ryu, J., Kim, H., Bae, S., Jung, C. and Gong, S.: Isolation and in Vitro Culture of Primary Cell Populations Derived from Ovarian Tissues of
the Rockfish, Sebastes schlegeli, Fish Aquatic Sci 19,
, 2016 |
Frog
|
Xenopus Laevis, female |
Oocytes |
Collagenase Type 1: 0.2%
|
Barth's solution |
Nicoll, G, Jawad, A., Weymouth, R, Zhang, H. and Beg, A.: Pharmacological characterization of the excitatory ‘Cys-loop’ GABA receptor family in Caenorhabditis elegans, Br J Pharmacol 174,
781, 2017 |
Frog
|
Xenopus laevis |
Oocytes |
Collagenase: 0.2%
|
Barth's solution |
Chatzidaki, A., D'Oyley, J., Gill-Thind, J., Sheppard, T. and Millar, N.: The Influence of Allosteric Modulators and Transmembrane Mutations on Desensitisation and Activation of alpha7 Nicotinic Acetylcholine Receptors., Neuropharmacol 97,
75-85, 2015 |
Frog
|
Xenopus |
Oocytes |
Collagenase Type 2: 0.2%
|
see reference |
O'Connell, D., Mruk, K., Rocheleau, J. and Kobertz, W.: Xenopus laevis Oocytes Infected with Multi-drug-resistant Bacteria: Implications for Electrical Recordings., J Gen Physiol 138,
271-7, 2011 |
Frog
|
Xenopus laevis, female |
Oocytes |
Collagenase: 0.2%
|
see reference |
Mruk, K. and Kobertz, W.: Discovery of a Novel Activator of KCNQ1-KCNE1 K Channel Complexes., PLoS ONE
4,
e4236, 2009 |
Frog
|
Xenopus laevis, female |
Oocytes |
Collagenase: 0.5%
|
Barth's solution, CF |
Cohen, S., Au, S. and Pante, N.: Microinjection of Xenopus Laevis Oocytes., J Vis Exp
24,
1106, 2009 |
Frog
|
Xenopus laevis, female |
Oocytes |
Collagenase Type 1: 0.2%
|
see reference |
Pannaccione Anna, Castaldo Pasqualina, Ficker Eckhard, Annunziato Lucio, Taglialatela Maurizio: Histidines 578 and 587 in the S5-S6 linker of the human Ether-a-gogo Related Gene-1 K+ channels confer sensitivity to reactive oxygen species, J Biol Chem 277,
8912-9, 2002 |
Frog
|
Xenopus laevis, female |
Oocytes |
Collagenase Type 1: 0.2%
|
CF Medium |
Alagem, N., Dvir, M., and Reuveny, E.: Mechanism of Ba2+ Block of a Mouse Inwardly Rectifying K+ Channel: Differential Contribution by Two Discrete Residues, J Physiol
534 (2),
381, 2001 |
Frog
|
Xenopus |
Oocytes |
Collagenase Type 1: 1%
|
(see reference) |
Tian, J., Kim, S., Heilig, E., and Ruderman, J.: Identification of XPR-1, A Progesterone Receptor Required for Xenopus Oocyte Activation, Proc Natl Acad Sci U S A 97,
14358, 2000 |
Frog
|
Xenopus laevis, female |
Oocytes |
Collagenase: 0.1%
|
Barth's solution, CF |
Karkanias, N, and Papke, R.: Subtype-Specific Effects of Lithium on Glutamate Receptor Function, J Neurophysiol 81,
1506, 1999 |
Frog
|
Xenopus laevis, mature female |
Oocytes |
Collagenase: 0.2%
|
CF Medium |
Moriarty, T., Gillo, B., Carty, D., Premont, R., Landau, E., Iyengar, R.: Beta gamma Subunits of GTP-Binding Proteins Inhibit Muscarinic Receptor Stimulation of Phospholipase C, Proc Natl Acad Sci U S A 85,
8865, 1988 |
Hamster
|
Hamster, Chinese |
Ovary |
Trypsin: 5%
|
Dialyzed fetal calf serum, 10% and 0.5M Methotrexate |
Wallis, R., and Drickamer, K.: Molecular Determinants of Oligomer Formation and Complement Fixation in Mannose-Binding Proteins, J Biol Chem 274 (6),
3580, 1999 |
Human
|
Human |
Endometrial epithelial and stromal fibroblasts |
Collagenase Type 1: 0.32%
Hyaluronidase: 62 u/ml
|
DMEM |
Chen, J. and Roan, N.: Isolation and Culture of Human Endometrial Epithelial Cells and Stromal Fibroblasts., Bio Protoc
5,
, 2015 |
Human
|
Human |
Endometrial stromal |
Collagenase Type 3: 8 u/ml
Deoxyribonuclease I: 0.01%
|
DMEM/F-12 |
Evans, J. and Salamonsen, L.: Decidualized Human Endometrial Stromal Cells are Sensors of Hormone Withdrawal in the Menstrual Inflammatory Cascade., Biol Reprod 90,
14, 2014 |
Human
|
Human |
Endothelial colony forming |
Collagenase Type 1: 0.1%
Deoxyribonuclease I: 0.1%
Neutral Protease: 0.075%
|
HBSS |
Patel, J., Seppanen, E., Chong, M., Yeo, J., Teo, E., Chan, J., Fisk, N. and Khosrotehrani, K.: Prospective Surface Marker-Based Isolation and Expansion of Fetal Endothelial Colony-Forming Cells from Human Term Placenta., Stem Cells Transl Med 2,
839-47, 2013 |
Human
|
Human |
Uterine epithelial |
Pancreatin: 0.34%
Hyaluronidase: 0.01%
Collagenase: 0.16%
|
HBSS |
Patel, M., Ghosh, M., Fahey, J. and Wira, C.: Uterine Epithelial Cells Specifically Induce Interferon-Stimulated Genes in Response to Polyinosinic-Polycytidylic Acid Independently of Estradiol., PLoS ONE
7,
e35654, 2012 |
Human
|
Human |
Mesenchymal stem |
Collagenase Type 1: 0.4%
Deoxyribonuclease I: 0.01%
|
DMEM/F12 |
Shalini, V., Pratheep, S., Muhammad, A., Sharmili, V., Elizabeth, G. and Rajesh, R.: Generation and Characterisation of Human Mesenchymal Stem Cells Derived From Umbilical Cord and Placenta, Regenerative Research 1,
48, 2012 |
Human
|
Human |
Mesenchymal stem |
Collagenase Type 2: 10%
Neutral Protease: see reference
|
CMF-DPBS |
Steigman, S. and Fauza, D.: Isolation of Mesenchymal Stem Cells from Amniotic Fluid and Placenta., Curr Protoc Stem Cell Biol
Chapter 1,
Unit 1E.2, 2007 |
Human
|
Human, female |
Decidual |
Collagenase: 0.25%
Deoxyribonuclease I: 6.25 u/ml
|
DMEM/F12 |
Lockwood, C., Arcuri, F., Toti, P., Felice, C., Krikun, G., Guller, S., Buchwalder, L. and Schatz, F.: Tumor Necrosis Factor-Alpha and Interleukin-1 Beta Regulate Interleukin-8 Expression in Third Trimester Decidual Cells: Implications for the Genesis of Chorioamnionitis., Am J Pathol 169,
1294-302, 2006 |
Human
|
Human |
Extravillanous trophoblasts and decidual stromal |
Trypsin: 0.1-0.25%
Collagenase Type 1: 0.1%
Deoxyribonuclease I: 0.02-0.05%
|
HBSS RPMI |
Spessotto, P., Bulla, R., Danussi, C., Radillo, O., Cervi, M., Monami, G., Bossi, F., Tedesco, F., Doliana, R. and Colombatti, A.: EMILIN1 Represents a Major Stromal Element Determining Human Trophoblast Invasion of the Uterine Wall., J Cell Sci
119,
4574, 2006 |
Human
|
Human |
Sertoli cells |
Trypsin: 2.5%
Collagenase Type 1: 2%
Hyaluronidase: 1%
|
DMEM/F-12 |
Teng Yan, Xue Wu-jun, Ding Xiao-ming, Feng Xin-shun, Xiang He-li, Jiang Ya-zhuo, Tian Pu-xun: Isolation and culture of adult Sertoli cells and their effects on the function of co-cultured allogeneic islets in vitro, Chin Med J (Engl)
118,
1857-62, 2005 |
Human
|
Human, female |
Uterine epithelial |
Pancreatin: 0.34%
Hyaluronidase: 0.01%
Collagenase: 0.16%
|
HBSS |
Meter, R., Wira, C. and Fahey, J.: Secretion of Monocyte Chemotactic Protein-1 by Human Uterine Epithelium Directs Monocyte Migration in Culture., Fertil Steril
84,
191, 2005 |
Human
|
Human, female, 34-51 yr |
Endometrial epithelial and stromal cells |
Collagenase Type 3: 0.03%
Deoxyribonuclease I: 0.004%
|
DMEM/F-12 |
Chan Rachel W S, Schwab Kjiana E, Gargett Caroline E: Clonogenicity of human endometrial epithelial and stromal cells, Biol Reprod 70,
1738-50, 2004 |
Human
|
Human |
Endothelial placental |
Collagenase Type 1: 0.2%
Trypsin: 0.2%
Deoxyribonuclease I: 0.1%
|
DMEM |
Wang Xin, Athayde Neil, Trudinger Brian: Microvascular endothelial cell activation is present in the umbilical placental microcirculation in fetal placental vascular disease, Am J Obstet Gynecol
190,
596-601, 2004 |
Human
|
Human |
Amnion epithelial and fibroblast |
Trypsin: 0.125%
Collagenase: 0.1%
Deoxyribonuclease I: 0.02%
|
PBS |
Sun Kang, Myatt Leslie: Enhancement of glucocorticoid-induced 11beta-hydroxysteroid dehydrogenase type 1 expression by proinflammatory cytokines in cultured human amnion fibroblasts, Endocrinology 144,
5568-77, 2003 |
Human
|
Human |
Uterine epithelial cells |
Pancreatin: 0.34%
Collagenase: 0.16%
Hyaluronidase: 0.16%
|
HBSS |
Fahey John V, Wira Charles R: Effect of menstrual status on antibacterial activity and secretory leukocyte protease inhibitor production by human uterine epithelial cells in culture, J Infect Dis 185,
1606-13, 2002 |
Human
|
Human, embryo |
Endothelial Hematopoietic Stromal |
Collagenase Type 1/2/4: 0.1%
|
DMEM |
Oberlin, E., Tavian, M., Blazsek, I., and Peault, B.: Blood-forming potential of vascular endothelium in the human embryo, Development
129,
4147, 2002 |
Human
|
Human, female |
Mesothelial |
Collagenase Type 1: 0.1%
Deoxyribonuclease I: 0.05%
|
Eagle's MEM |
Witz Craig A, Allsup Karen T, Montoya-Rodriguez Iris A, Vaughn Shelby L, Centonze Victoria E, Schenken Robert S: Culture of menstrual endometrium with peritoneal explants and mesothelial monolayers confirms attachment to intact mesothelial cells, Hum Reprod 17,
2832-8, 2002 |
Human
|
Human |
Stem, embryonic |
Neutral Protease: 0.01% - 0.02%
|
DMEM |
Zhang, S., Wernig, M., Duncan, I., Brustle, O., and Thomson, J.: In Vitro Differentiation of Transplantable Neural Precursors from Human Embryonic Stem Cells, Nat Biotechnol
19,
1129, 2001 |
Human
|
Human, 20-40 yr |
Endometrium epithelial and stromal cells |
Collagenase Type 1: 0.2%
|
HBSS |
Arnold, J., Kaufman, D., Seppala, M., and Lessey, B.: Endometrial stromal cells regulate epithelial cell grownth in vitro: a new co-culture model, Hum Reprod 16,
836, 2001 |
Human
|
Human |
Chorionic villi |
Trypsin: see reference
Collagenase Type 3: 100 u/ml
|
HBSS |
Yusuf RZ, Naeem R: Cytogenetic studies of spontaneous miscarriages: a seven year study to compare significance of primary vs. secondary culture methods for assessment of fetal karyotype yield and maternal cell contamination, Early Pregnancy 5,
121-31, 2001 |
Human
|
Human |
Endometrial endothelial cells |
Collagenase Type 1: 0.2%
|
McCoys medium |
Nikitenko LL, MacKenzie IZ,Rees MC, Bicknell R: Adrenomedullin is an autocrine regulator of endothelial growth in human endometrium, Mol Hum Reprod 6,
811, 2000 |
Human
|
Human, female, 20-40 year |
Microvascular endothelial cells |
Collagenase Type 2: 0.2%
Deoxyribonuclease I: 0.0015%
Trypsin: 0.05%
|
PBS |
Gargett CE, Bucak K, Rogers PA: Isolation, characterization and long-term culture of human myometrial microvascular endothelial cells, Hum Reprod 15,
293-301, 2000 |
Human
|
Human, female |
Endometrial epithelial cells |
Collagenase Type 3: 45 u/ml
Deoxyribonuclease I: .00035%
|
DMEM/F-12 |
Zhang J, Lathbury LJ, Salamonsen LA: Expression of the chemokine eotaxin and its receptor, CCR3, in human endometrium, Biol Reprod 62,
404-11, 2000 |
Human
|
Human |
Theca cells |
Collagenase Type 1: 0.3%
Deoxyribonuclease I: 0.0005%
Hyaluronidase: 0.1%
|
PBS |
Runesson E, Ivarsson K, Janson P, Brannstrom M: Gonadotropin- and cytokine-regulated expression of the chemokine interleukin 8 in the human preovulatory follicle of the menstrual cycle, J Clin Endocrinol Metab 85,
4387-95, 2000 |
Human
|
Human |
Corpus luteum cells |
Collagenase Type 2: 0.25%
Deoxyribonuclease I: .005%
|
PBS |
Friden BE, Runesson E, Hahlin M, Brannstrom M: Evidence for nitric oxide acting as a luteolytic factor in the human corpus luteum, Mol Hum Reprod 6,
397-403, 2000 |
Human
|
Human, females, 25-45 yr |
Luteal cells |
Collagenase Type 2: 0.25%
Deoxyribonuclease I: 0.005%
|
PBS |
Friden BE, Hagstrom H, Lindblom B, Sjoblom P, Wallin A, Brannstrom M, Hahlin M: Cell characteristics and function of two enriched fraction of human luteal cells prolonged culture, Mol Hum Reprod 5,
714-9, 1999 |
Human
|
Human, female, 25-41 year |
Follicles |
Collagenase Type 2: 0.025-0.1%
|
EBSS |
Hovatta O, Wright C, Krausz T, Hardy K, Winston RM: Human primordial, primary and secondary ovarian follicles in long-term culture: effect of partial isolation, Hum Reprod 14,
2519-24, 1999 |
Human
|
Human |
Placental |
Deoxyribonuclease I: 0.04%
Collagenase Type 2: 0.1%
|
PSS |
Bradbury., R., Sunn, K., Crossley, M., Bai, M., Brown, E., Delbridge, D., and Conigrave, A.: Expression of the Parathyroid Ca 2+ - Sensing Receptor in Cytotrophoblasts From Human Term Placenta, J Endocrinol 156,
425-430, 1998 |
Human
|
Human, female |
Endometrial stromal cells |
Collagenase: 4000 u/ml
|
DMEM/F-12 |
Huang JC, Liu DY, Dawood MY: The expression of vascular endothelial growth factor isoforms in cultured human endometrial stromal cells and its regulation by 17beta-oestradiol, Mol Hum Reprod 4,
603-7, 1998 |
Human
|
Human |
Stromal endometrial |
Collagenase Type 3: 45 u/ml
Deoxyribonuclease I: .00035%
|
DMEM/Ham's F12 |
Zhang J, Nie G, Jian W, Woolley DE, Salamonsen LA: Mast cell regulation of human endometrial matrix metalloproteinases: A mechanism underlying menstruation, Biol Reprod 59,
693-703, 1998 |
Human
|
Human |
Epithelial, fallopian tube |
Collagenase Type 1: 1%
|
Medium 199 |
Takeuchi, K., Maruyama, I., Yamamoto, S., Oki, T., Nagata, Y.: Isolation and Monolayer Culture of Human Fallopian Tube Epithelial Cells, In Vitro Cell Dev Biol 27,
720, 1991 |
Human
|
Human |
Trophoblasts, placental |
Trypsin: 0.25%
|
EBSS, CMF |
Branchaud, C.L., Goodyer, C.G., Guyda, H.J. and Lefebvre, Y.: A Serum-Free System for Culturing Human Placental Trophoblasts, In Vitro Cell Dev Biol 26,
865, 1990 |
Human
|
Human |
Trophoblasts, placental |
Trypsin: 0.25%
|
PBS |
Jie, Z., Fey, S., Hager, H., Hollsberg, P., Ebbesen, P., and Larsen, P.: Markers For Human Placental Trophoblasts in Two-Dimensional Gel Electrophoresis, In Vitro Cell Dev Biol 26,
937, 1990 |
Human
|
Human |
Chorionic, placental |
Deoxyribonuclease I: 0.003%
|
HBSS |
Egan, D., Grzegorczyk, V., Tricarico, K., Rueter, A.H., Olleman, W., and Marcotte, P.: Human Placental Chorionic Renin: Production, Purification and Characterization, Biochim Biophys Acta 965,
68, 1988 |
Human
|
Human, female |
Endometrial |
Collagenase: 2%
|
RPMI 1640 |
Rinehart, C., Lyn-Cook, B., and Kaufman, D.: Gland formation from human endometrial epithelial cells in vitro, In Vitro Cell Dev Biol 24 (10),
1037, 1988 |
Human
|
Human |
Placental |
Trypsin: 0.25%
|
DMEM |
Morrish, D., and Siy, O.: Critical Factors in Establishing Monolayer Cultures of Normal Human Placental Cells in Serum-Free Medium, Endocr Res 12 (3),
229, 1986 |
Human
|
Human (also porcine, bovine) |
Interna & corpus luteum Endometrium Ovarian Uterine |
Pronase: 0.1%
|
Moscona's BSS |
Marcus, G., Connor, L., Domingo, M., Tsang, B., Downey, B., and Ainsworth, L.: Enzymatic Dissociation of Ovarian and Uterine Tissues, Endocr Res 10,
151, 1984 |
Human
|
Human, female, 27-49 years |
Epithelial Ovary |
Trypsin: 0.125%
|
HBSS, CMF |
Auersperg, N., Siemens, C.H., and Myrdal, S.E.: Human Ovarian Surface Epithelium In Primary Culture, In Vitro 20,
743, 1984 |
Human
|
Human |
Epithelial Stromal |
Collagenase: 0.25%
|
See reference |
Siegfried, J., Nelson, K., Martin, J., and Kaufman, D.: Histochemical Identification of Cultured Cells From Human Endometrium, In Vitro 20 (1),
25, 1984 |
Human
|
Human, infant and neonate |
Epithelial Prostate |
Trypsin: 0.1%
|
HBSS |
Lechner, J., Babcock, M., Marnell, M., Narayan, K., and Kaighn, M.: Normal Human Prostate Epithelial Cell Cultures, Methods Cell Biol 21,
195, 1980 |
Human
|
Human, female, 27 years |
Smooth muscle, uterine |
Trypsin: 0.05%
|
EBSS |
Rifas, L., Fant, J., Makman, M., and Seifter, S.: The Characterization of Human Uterine Smooth Muscle Cells in Culture, Cell Tissue Res 196,
385, 1979 |
Human
|
Human |
Epithelial Stromal |
Collagenase Type 1: 180 u/ml
|
DMEM |
Kirk, D., King, R., Heyes, J., Peachey, L., Hirsch, P., and Taylor, W.: Normal Human Edometrium in Cell Culture, In Vitro 14 (8),
651, 1978 |
Insect
|
Drosophila |
Ovarian |
Collagenase Type 2: 0.1%
|
Grace's |
Ma, X., Wang, S., Do, T., Song, X., Inaba, M., Nishimoto, Y., Liu, L., Gao, Y., Mao, Y., Li, H., McDowell, W., Park, J., Malanowski, K., Peak, A., Perera, A., Li, H. et al.: Piwi is Required in Multiple Cell Types to Control Germline Stem Cell Lineage Development in the Drosophila Ovary., PLoS ONE
9,
e90267, 2014 |
Insect
|
Drosophila, embryonic |
Cardiac differentiating |
Collagenase Type 1: 20 u/ml
Trypsin: 0.25%
Deoxyribonuclease I: 4 u/ml
|
Schneider |
Salmand, P. Iche-Torres, M. and Perrin, L.: Tissue-Specific Cell Sorting from Drosophila Embryos: Application to Gene Expression Analysis.,Fly (Austin) Vol. 5,,
,
261, |
Monkey
|
Rhesus macaque |
Follicle |
Collagenase Type 1: 275 u/m
Deoxyribonuclease I: 585 u/ml
|
HEPES |
Xu, J, Lawson, MS, Yeoman, RR, Pau, KY, Barrett, SL, Zelinski, MB and Stouffer, RL: Secondary Follicle Growth and Oocyte Maturation During Encapsulated Three-Dimensional Culture in Rhesus Monkeys: Effects of Gonadotrophins, Oxygen and Fetuin., Hum Reprod 26,
1061-72, 2011 |
Mouse
|
Mouse, 4 week |
Trophoblast |
Collagenase Type 1: 0.1%
Collagenase Type 2: 0.25%
|
DMEM |
Hou, M., Han, J., Li, G., Kwon, M., Jiang, J., Emani, S., Taglauer, E., Park, J., Choi, E., Vodnala, M., Fong, Y., Emani, S., Rosas, I., Perrella, M. and Liu, X.: Multipotency of Mouse Trophoblast Stem Cells, Stem Cell Res Ther 11,
55, 2020 |
Mouse
|
Mouse, adult |
Testes |
Collagenase Type 1: 120 u/ml
Deoxyribonuclease I: 0.1%
Trypsin: 5%
|
MEM |
Lee, A, Rusch, J., Lima, A., Usmani, A., Huang, N., Lepamets, M., Vigh-Conrad, K., Worthington, R., et al: Rare Mutations in the Complement Regulatory Gene CSMD1 are Associated with Male and Female Infertility., Nat Commun
10,
4626, 2019 |
Mouse
|
Mouse, 1-7 day |
Testicular |
Collagenase Type 4: 0.1%
Deoxyribonuclease I: 0.1%
|
HBSS |
Kang, H., Chen, L., Lichti-Kaiser, K., Liao, G., Gerrish, K., Bortner, C., Yao, Hu., Eddy, E. and Jetten, A.: Transcription Factor GLIS3: A New and Critical Regulator of Postnatal Stages of Mouse Spermatogenesis., Stem Cells 34,
2772-2783, 2016 |
Mouse
|
Mouse, adult |
Spermatogonial stem |
Trypsin: 0.05%
Collagenase Type 1: 0.03%
Deoxyribonuclease I: 80 u/ml
|
DMEM |
Martin, L. and Seandel, M.: Serial Enrichment of Spermatogonial Stem and Progenitor Cells (SSCs) in Culture for Derivation of Long-Term Adult Mouse SSC Lines., J Vis Exp
72,
e50017, 2013 |
Mouse
|
Mouse |
Ovarian |
Collagenase Type 4: 800 u/ml
|
HBSS |
White, Y., Woods, D., Takai, Y., Ishihara, O., Seki, H. and Tilly, J.: Oocyte Formation by Mitotically Active Germ Cells Purified from Ovaries of Reproductive-Age Aomen., Nat Med 18,
413, 2012 |
Mouse
|
Mouse, female |
Lymphocytes |
Collagenase: 450 u/ml
|
RPMI |
Jiang, J. and Kelly, K.: Isolation of Lymphocytes from Mouse Genital Tract Mucosa., J Vis Exp
67,
e4391, 2012 |
Mouse
|
Mouse |
Vaginal smooth muscle |
Collagenase Type 2: 175 u/ml
Elastase: 0.025%
|
DMEM/F12 |
Venkataraman, L., Lenis, A., Couri, B., Damaser, M. and Ramarurthi, A: Induced Regenerative Elastic Matrix Repair in LOXL 1 Knockout Mouse Cell Cultures: Towards Potential Therapy for Pelvic Organ Prolapse, J Tissue Sci Eng 3,
3, 2012 |
Mouse
|
Mouse, male |
Testis, meiotic |
Collagenase Type 1: 120 u/ml
Deoxyribonuclease I: 0.001%
Trypsin: 0.1%
|
Gey's BSS |
Getun, I., Torres, B. and Bois, P.: Flow Cytometry Purification of Mouse Meiotic Cells., J Vis Exp
50,
2602, 2011 |
Mouse
|
Mouse |
Zona pellucid free eggs |
Collagenase: 0.1%
|
THY-HEPES |
Yamatoya, K., Ito, C., Araki, M., Furuse, R. and Toshimori, K.: One-Step Collagenase Method for Zona Pellucida Removal in Unfertilized Eggs: Easy and Gentle Method for Large-Scale Preparation., Reprod Med Bio 10,
97-103, 2011 |
Mouse
|
Mouse |
Uterine stomal |
Neutral Protease: 0.6%
Pancreatin: 0.25%
Collagenase Type 3: 0.05%
|
DMEM/F12 |
Chen, L., Belton, R. and Nowak, R.: Basigin-Mediated Gene Expression Changes in Mouse Uterine Stromal Cells During Implantation., Endocrinology 150,
966, 2009 |
Mouse
|
Mouse |
Germ cells |
Collagenase Type 1: 100 u/ml
|
HBSS |
Breault, D., Min, I., Carlone, D., Farilla, L., Ambruzs, D., Henderson, D., Algra, S., Montgomery, R., Wagers, A. and Hole, N.: Generation of mTert-GFP Mice as a Model to Identify and Study Tissue Progenitor Cells., Proc Natl Acad Sci U S A 105,
10420, 2008 |
Mouse
|
Mouse, embryonic |
Hematopoietic stem cells |
Collagenase: 0.1%
|
PBS |
Gekas, C., Rhodes, K. and Mikkola, H.: Isolation and Analysis of Hematopoietic Stem Cells from the Placenta., J Vis Exp
16,
e742, 2008 |
Mouse
|
Mouse, 6 day |
Sertoli |
Collagenase Type 2: 500 u/ml
Hyaluronidase: 0.1%
Deoxyribonuclease I: 0.0005%
|
DMEM |
Nalbandian Angele, Dettin Luis, Dym Martin, Ravindranath Neelakanta: Expression of vascular endothelial growth factor receptors during male germ cell differentiation in the mouse, Biol Reprod 69,
985-94, 2003 |
Mouse
|
Mouse, adult, neonatal and fetal |
Testicular cells |
Collagenase Type 1: 0.1%
|
DMEM/F-12 |
O'Shaughnessy PJ, Fleming LM, Jackson G, Hochgeschwender U, Reed P, Baker PJ: Adrenocorticotropic hormone directly stimulates testosterone production by the fetal and neonatal mouse testis, Endocrinology 144,
3279-84, 2003 |
Mouse
|
Mouse, male, 3.5 months old |
Leydig |
Collagenase: 0.06%
|
Medium E 199 |
Faldikova, L., Diblikova, I., Canderle, J., Zraly, Z., Veznik, Z., and Sulcova, A.: Effects of Nutrition, Social Factors and Chronic Stress on the Mouse Leydig Cell Testosterone Production, Vet Med 46 (6),
160, 2001 |
Mouse
|
Mouse, male, 12-14-week-old |
Seminiferous tubules |
Trypsin: 0.05%
|
DMEM |
Lin, Q., Sirotkin, A., and Skoultchi, A.: Normal Spermatogenesis in Mice Lacking the Testis-Specific Linker Histone H1t, Mol Cell Biol
20 (6),
2122, 2000 |
Mouse
|
Mouse, 6-12 day |
Oocyte-granulosa |
Collagenase Type 1: 0.1%
Deoxyribonuclease I: 0.02%
|
Waymouth |
Eppig JJ, O'Brien MJ: Development in vitro of mouse oocytes from primordial follicles, Biol Reprod 54,
197-207, 1996 |
Mouse
|
Mouse, female,6-8 weeks |
Uterine |
Trypsin: 0.2%
Trypsin: 0.2%
|
HBSS |
Ghosh, D., Danielson, K., Alston, J., and Heyner, S.: Functional Differentiation of Mouse Uterine Epithelial Cells Grown on Collagen Gels or Reconstituted Basement Membranes, In Vitro Cell Dev Biol 27A,
713, 1991 |
Mouse
|
Mouse, female, 21 day, 10-12 g |
vaginal epithelial |
Collagenase Type 3: 38 u/ml
|
DMEM |
Tsai, P., Uchima, F., Hamamoto, S., and Bern, H.: Proliferation and Differentiation of Prepubertal Mouse Vaginal Epithelial Cells In Vitro and the Specificity of Estrogen-Induced Growth Retardation, In Vitro Cell Dev Biol 27A,
461, 1991 |
Mouse
|
Mouse, CF1, female |
Cumulus, one-cell embryos |
Hyaluronidase: 0.1%
|
PBS, CMF |
Spindle, A.: In vitro Development of One-Cell Embryos from Outbred Mice: Influence of Culture Medium Composition, In Vitro Cell Dev Biol 26,
151, 1990 |
Mouse
|
Mouse (BALB/cCRGL), male, 2-3 months |
Epithelial, prostate gland |
Hyaluronidase: 0.1%
|
Medium 199 |
Turner, T., Bern, H., Young,P., and Cunha, G.: Serum-Free Culture of Enriched Mouse Anterior and Ventral Prostatic Epithelial Cells in Collagen Gel, In Vitro Cell Dev Biol 26,
722, 1990 |
Mouse
|
Mouse (C57B/T), fetus, 16-17 days old |
Prostate |
Trypsin: 1.0%
|
HBSS/ DMEM |
Thompson, T.C., Southgate, J., Kitchener, G., and Land, H.: Multistage Carcinogenesis Induced by ras and myc Oncogenes in a Reconstituted Organ, Cell
56,
917, 1989 |
Mouse
|
Mouse, outbred, CD-1, 21-23 days old |
Epithelial |
Trypsin: 0.5%
|
Medium 199 |
Tomooka, Y., DiAugustine, R., and McLachlan, J.: Proliferation of Mouse Uterine Epithelial Cells in Vitro, Endocrinology 118 (3),
1011, 1986 |
Mouse
|
Mouse, BALB/c |
Epithelial Mesencymal |
Trypsin: 1%
|
DMEM |
Bigsby, R., Cooke, P., and Cunha, G.: A Simple Efficient Method For Separating Murine Uterine Epithelial and Mesenchymal Cells, Am J Physiol 251,
E630, 1986 |
Mouse
|
Mouse, BALB/cCrg, 40 days old |
Epithelial |
Trypsin: 1%
|
Medium 199 |
Cooke, P., Uchima, F., Fujii, D., Bern, H., and Cunha, G.: Restoration of Normal Morphology and Estrogen Responsiveness in Cultured Vaginal and Uterine Epithelia Transplanted with Stroma, Proc Natl Acad Sci U S A 83,
2109, 1986 |
Mouse
|
Mouse, male, 10-13 wk |
Leydig Testis |
Deoxyribonuclease I: 0.001%
|
Medium 199 w/ BSA |
Stalvcey, J. and Payne, A.: Luteinizing Hormone Receptors and Testosterone Production in Whole Testes and Purified Leydig Cells from the Mouse: Differences among Inbred Strains, Endocrinology 112,
1696, 1983 |
Mouse
|
Mouse (BALB/cCrgl), female, 50 - 60 days |
Epithelial, vagina |
Collagenase Type 3: 0.1%
|
HBSS |
Iguchi, T., Uchima, F.A., Ostrander, P., and Bern, H.: Growth of Normal Mouse Vaginal Epithelial Cells in and on Collagen Gels, Proc Natl Acad Sci U S A 80,
3743, 1983 |
Ovine
|
Ovine, 3-5 year |
Endometrial stromal |
Collagenase Type 1: 0.5%
Deoxyribonuclease I: 0.04 u/ml
|
DMEM/F-12 |
Letouzey, V., Tan, K., Deane, J., Ulrich, D., Gurung, S., Ong, Y. and Gargett, C.: Isolation and Characterisation of Mesenchymal Stem/Stromal Cells in the Ovine Endometrium., PLoS ONE
10,
e0127531, 2015 |
Ovine
|
Sheep |
Epithelial |
Collagenase: 125 - 190 u/ml
|
DMEM |
Salamonsen, L., Sum O, W., Doughton, B., and Findlay, J.: The Effects of Estrogen and Progesterone In Vivo on Protein Synthesis and Secretion by Cultured Epithelial Cells from Sheep Endometrium, Endocrinology 117 (5),
2148, 1985 |
Porcine
|
Porcine, 1-4 day |
Testicular |
Collagenase Type 4: 0.1%
Hyaluronidase: 0.1%
Trypsin: 0.25%
|
DMEM |
Park, M., Park, J., Kim, M., Lee, K., Park, H., Yun, J., Choi, J., Lee, E. and Lee, S.: Development of a High-Yield Technique to Isolate Spermatogonial Stem Cells From Porcine Testes., J Assist Reprod Genet
31,
983-91, 2014 |
Porcine
|
Porcine, male, 8 day |
Seminiferous epithelial cells |
Collagenase: 0.15%
Deoxyribonuclease I: .0001%
Hyaluronidase: 0.15%
Trypsin: 0.05%
|
DMEM/F12 |
Dirami G, Ravindranath N, Pursel V, Dym M: Effects of stem cell factor and granulocyte macrophage-colony stimulating factor on survival of porcine type A spermatogonia cultured in KSOM, Biol Reprod 61,
225-30, 1999 |
Porcine
|
Porcine, female |
Corpus Leuteum |
Collagenase Type 4: 600 u/ml
|
Medium 199 |
Ciereszko, R., Petroff, B., Ottobre, A., Guan, Z., Stokes, B., and Ottobre, J.: Assessment of the Mechanism by Which Prolactin Stimulates Progesterone Production by Early Corpora Lutea of Pigs, J Endocrinol 159,
201, 1998 |
Porcine
|
Porcine (also bovine, human) |
Interna & corpus luteum Endometrium Ovarian Uterine |
Pronase: 0.1%
|
Moscona's BSS |
Marcus, G., Connor, L., Domingo, M., Tsang, B., Downey, B., and Ainsworth, L.: Enzymatic Dissociation of Ovarian and Uterine Tissues, Endocr Res 10,
151, 1984 |
Porcine
|
Porcine, 3-4 week |
Leydig Testis |
Trypsin: 0.0003%
|
Lebovitz L-15 Medium |
Mather, J., Saez, J., and Haour, F.: Regulaton of Gonadotropin Receptors and Steroidogenesis in Cultured Porcine Leydig Cells, Endocrinology 110,
933, 1982 |
Rabbit
|
Rabbit, New Zealand, 3 month |
Testicular germ |
Collagenase Type 1: 0.1%
Trypsin: 0.25%
Deoxyribonuclease I: 0.7%
|
HBSS |
Kubota, H., Wu, X., Goodyear, S., Avarbock, M. and Brinster, R.: Glial Cell Line-Derived Neurotrophic Factor and Endothelial Cells Promote Self-Renewal of Rabbit Germ Cells with Spermatogonial Stem Cell Properties., FASEB J
25,
2604, 2011 |
Rabbit
|
Rabbit, New Zealand, 3 month |
Testicular germ |
Collagenase Type 1: 0.1%
Trypsin: 0.25%
Deoxyribonuclease I: 0.7%
|
HBSS |
Kubota, H., Wu, X., Goodyear, S., Avarbock, M. and Brinster, R.: Glial Cell Line-Derived Neurotrophic Factor and Endothelial Cells Promote Self-Renewal of Rabbit Germ Cells with Spermatogonial Stem Cell Properties., FASEB J
25,
2604, 2011 |
Rabbit
|
Rabbit, New Zealand white, 4-6 month old, |
Ovarian Mesothelial |
Collagenase Type 1: 300 u/ml
|
HBSS |
Setrakian, S., Oliveros-Saunders, B., and Nicosia, S.: Growth Stimulation of Ovarian and Extraovarian Mesothelial Cells by Corpus Luteum Extract, In Vitro Cell Dev Biol 29A,
879, 1993 |
Rabbit
|
Rabbit, New Zealand white, adult, female (nonpregnant), 3-4 kg |
Myocytes, uterine |
Deoxyribonuclease I: 200 µg/ml
|
HBSS-HEPES buffer |
Phillippe, M., Saunders, T., and Bangalore, S.: Alpha-1,Alpha-2,and Beta Adrenergic Signal Transduction in Cultured Uterine Myocytes, In Vitro Cell Dev Biol 26,
369, 1990 |
Rabbit
|
Rabbit, New Zealand, mature, female, 4-5 Kg |
Ovarian surface epithelial and peritoneal mesothelial |
Collagenase Type 1: 300 IU/ml (280 IU/mg)
|
HBSS, CMF |
Piquette, G., and Timms, B.: Isolation and Characterization of Rabbit Ovarian Surface Epithelium, Granulosa Cells, and Peritoneal Mesothelium in Primary Culture, In Vitro Cell Dev Biol 26,
471, 1990 |
Rabbit
|
Rabbit, mixed breed |
Endometrial epithelial |
Collagenase Type 1: 0.005%
|
DMEM |
Mulholland, J., Winterhager, E., and Beier, H.: Changes in Proteins Synthesized by Rabbit Endometrial Epithelial Cells Following Primary Culture, Cell Tissue Res 252,
123, 1988 |
Rabbit
|
Rabbit, New Zealand white, mature, 3-4 Kg |
Myometrial |
Trypsin: 0.02%, 0.03%, 0.0375%
|
HBSS |
Boulet, A., and Fortier, M.: Preparation and Characterization of Rabbit Myometrial Cells in Primary Culture: Influence of Estradiol and Progesterone Treatment, In Vitro Cell Dev Biol 23,
93, 1987 |
Rabbit
|
Rabbit, New Zealand white estrous, female, 4-5 months |
Mesothelial and surface epithelial Ovaries |
Trypsin: 0.125%-0.5%
|
Medium 199 |
Nicosia, S., Johnson, J., and Streibel, E.: Isolation and Ultrastructure of Rabbit Ovarian Mesothelium(Surface Epithelium), Int J Gynecol Pathol 3,
348, 1984 |
Rat
|
Rat |
Placental mesenchymal |
Collagenase Type 4: 0.1%
Deoxyribonuclease I: 0.02%
Neutral Protease: 0.1%
|
DMEM |
Jiang, H., Zhang, Y., Tian, K., Wang, B. and Han, S.: Amelioration of Experimental Autoimmune Encephalomyelitis Through Transplantation of Placental Derived Mesenchymal Stem Cells., Sci Rep
7,
41837, 2017 |
Rat
|
Rat, SD, 350-450 g |
Leydig cells |
Collagenase: .05-0.1%
|
Medium 199 |
Sharma RS, Pal PC, Rajalakshmi M.: Isolation and Culture of Leydig Cells from Adult Rats, Indian J Clinical Biochem 21,
27, 2006 |
Rat
|
Rat, SD, adult, male, 8 - 10 week |
Seminiferous tubules |
Trypsin: 0.05%
|
Krebs-Ringer bicarbonate buffer (see reference) |
Abou-Haila, A., and Tulsiani, D.: Acid Glycohydrolases in Rat Spermatocytes, Spermatids and Spermatozoa: Enzyme Activities, Biosynthesis and Immunolocalization, Biol Proced Online 3 (1),
35, 2001 |
Rat
|
Rat, SD, immature |
Ovary |
Collagenase Type 1: 144 u/ml
|
McCoy's 5a |
Ando, M., Kol, S., Irahara, M., Sirois, J., and Adashi, E.: Non-Steroidal Anti-Inflammatory Drugs (NSAIDS) Block the Late, Prostanoid-Dependent/Ceramide-Independent Component of Ovarian IL-1 Action: Implications for the Ovulatory Process, Mol Cell Endocrinol
157,
21, 1999 |
Rat
|
Rat, Han-Wistar, adult, male, 250 g |
Testes |
Trypsin: 0.1%
|
Medium 199 w/ Hank's salts |
Leckie, C., Welberg, L., and Seckl, J.: 11B-Hydroxysteroid Dehydrogenase is a Predominant Reductase in Intact Rat Leydig Cells, J Endocrinol 159,
233, 1998 |
Rat
|
Rat, SD, female, immature, 26 day (also rat, pregnant and pseudopregnant) |
Corpus luteum |
Neutral Protease: 2.4 u/ml Deoxyribonuclease: 200u/ml
|
Serum-free medium (see reference) |
Tellieria, C., Ou, J., Sugino, N., Ferguson, S., and Gibori, G.: The Expression of Interleukin-6 in the Pregnant Rat Corpus Luteum and Its Regulation by Progesterone and Glucocorticoid, Endocrinology 139 (8),
3597, 1998 |
Rat
|
Rat, SD, 25 day |
Ovarian theca-interstitial |
Collagenase Type 1: 0.5%
Deoxyribonuclease I: 0.02%
|
Medium 199 |
Duleba AJ, Spaczynski RZ, Olive DL, Behrman HR: Effects of insulin and insulin-like growth factors on proliferation of rat ovarian theca-interstitial cells, Biol Reprod 56,
891-7, 1997 |
Rat
|
Rat, SD, male, 90 days |
Sertoli |
Hyaluronidase: 0.1%
|
DMEM |
Onoda, M. and Djakiew, D.: Pachytene Spermatocyte Protein(s) Stimulate Sertoli Cells Grown in Bicameral Chambers: Dose-Dependent Secretion of Ceruloplasmin, Sulfated Glycoprotein-1, Sulfated Glycoprotein-2, and Transferrin, In Vitro Cell Dev Biol 27,
215, 1991 |
Rat
|
Rat, SD, male |
Leydig |
Trypsin: 0.02%
|
DMEM |
Abayasekara, D., Kurlak, L., Band, A., Sullivan, M., and Cooke, B.: Effect of Cell Purity, Cell Concentration, and Incubation Conditions on Rat Testis Leydig Cell Steroidogenesis, In Vitro Cell Dev Biol 27,
253, 1991 |
Rat
|
Rat, neonatal |
Uterine |
Trypsin: 1%
|
HBSS |
Branham, W., Lyn-Cook, B., Andrews, A., McDaniel, M., Sheehan, D.: Growth of Neonatal Rat Uterine Luminal Epithelium on Extracellular Matrix, In Vitro Cell Dev Biol 27,
442, 1991 |
Rat
|
Rat, SD, male, 120-160 g |
Leydig Adrenal |
Collagenase Type 2: 0.03% (adrenal)
|
Krebs Ringer bicarbonate buffer |
Ng, T. and Liu, W.: Toxic Effect of Heavy Metals on Cells Isolated from the Rat Adrenal and Testis, In Vitro Cell Dev Biol 26,
24, 1990 |
Rat
|
Rat, Wistar, adult |
Uterine |
Trypsin: 0.5%
|
PBS |
Pampfer, S., Vanderheyden, I., Michiels, B., and DeHertogh, R.: Co-Culture of Two-Cell Rat Embryos on Cell Monolayers, In Vitro Cell Dev Biol 26,
944, 1990 |
Rat
|
Rat, SD, 10 day |
Sertoli cells |
Collagenase Type 2: 500 u/ml
Deoxyribonuclease I: 0.0005%
Hyaluronidase: 0.1%
|
PBS |
Hadley MA, Weeks BS, Kleinman HK, Dym M: Laminin promotes formation of cord-like structures by Sertoli cells in vitro, Dev Biol 140,
318-27, 1990 |
Rat
|
Rat, immature |
Luteal, ovaries |
Collagenase: 0.3%
|
McCoy's |
Rajan, V. and Menon, K.: Differential Uptake and Metabolism of Free and Esterified Cholesterol from High-density Lipoproteins in the Ovary, Biochim Biophys Acta 959,
206, 1988 |
Rat
|
Rat, male, 20 day |
Sertoli |
Trypsin: 0.15%
|
(see reference) |
Skinner, M., Fetterolf, P., and Anthony, C.: Purification of a Paracrine Factor, P-Mod-S,Produced by Testicular Peritubular Cells That Modulates Sertoli Cell Function, J Biol Chem 263,
2884, 1988 |
Rat
|
Rat, SD, female |
Luminal epithelial |
Trypsin: 0.5%
|
HBSS |
Glasser, S., Julian, J., Decker, G., Tang, J., and Carson, D.: Development of Morphological and Functional Polarity in Primary Cultures of Immature Rat Uterine Epithelial Cells, J Cell Biol
107 (6),
2409, 1988 |
Rat
|
Rat, Wistar, virgin, female, 250 g |
Vaginal epithelial |
Trypsin: 0.5%
|
PBS |
Conti, C., and Tasat, D.: Regulation of Cultured Rat Vaginal Epithelial Cells By 17B-Estradiol and Progesterone, J Steroid Biochem 24 (3),
747, 1986 |
Rat
|
Rat, SD, female, 21 day |
Luteal, ovaries |
Deoxyribonuclease I: 0.0004%
|
McCoy's |
Rajan, V. and Menon, K.: Involvement of Microtubules in Lipoprotein Degradation and Utilization for Steroidogenesis in Cultured Rat Luteal Cells, Endocrinology 117,
2408, 1985 |
Rat
|
Rat, SD, male, 10 day |
Sertoli |
Trypsin: 0.025%
|
DMEM |
Hadley, M., Byers, S., Suarez-Quian, C., Kleinman, H., and Dym, M.: Extracellular Matrix Regulates Seratoli Cell Differentiation, Testicular Cord Formation and germ cell development in vitro, J Cell Biol
101,
1511, 1985 |
Rat
|
Rat, Holtzman, female, immature |
Uterine |
Deoxyribonuclease I: 0.025%
|
DMEM buffered with HEPES |
Kassis, J., Walent, J., and Gorski, J.: Estrogen Receptors in Rat Uterine Cell Cultures: Effects of Medium on Receptor Concentration, Endocrinology 115,
762, 1984 |
Rat
|
Rat, SD, adult,male, 50-70 days |
Testicular |
Deoxyribonuclease I: 10 µg/ml
|
HEPES |
Hsueh, A., Bambino, T., Zhuang, L., Welsh, T., and Ling, N.: Mechanism of the Direct Action of Gonadotropin Releasing Hormone and Its Antagonist on Androgen Biosynthesis by Cultured Rat Testicular Cells, Endocrinology 112,
1653, 1983 |
Rat
|
Rat, SD, female, pseudopregnant, 21 day |
Luteal, ovaries |
Hyaluronidase: 0.1%
|
EBSS |
Rajendran, K., Hwang, J., and Menon, K.: Binding, Degradation an Utilization of Plasma High Density and Low Density Lipoproteins for Progesterone Production In Cultured Rat Luteal Cells, Endocrinology 112,
1746, 1983 |
Rat
|
Rat, SD, 4-6 day, male |
Sertolli, seminiforous tubules |
Collagenase: 0.03%
|
Serum-free medium |
Rich, K., Bardin, C., Gunsalus, G., and Mather, J.: Age-Dependent Pattern of Androgen-Binding Protein Secretion from Rat Sertoli Cells in Primary Culture, Endocrinology 113,
2284, 1983 |
Rat
|
Rat, SD, female, 26 days |
Luteal |
Deoxyribonuclease I: 0.0004%
|
Medium 199 |
Azhar, S. and Reaven, E.: Effect of Antimicrotubule Agents on Microtubules and Steroidogenesis in Luteal Cells, Am J Physiol 243,
E380, 1982 |
Rat
|
Rat, SD, male |
Leydig |
Collagenase: 0.1%
|
Krebs Ringer bicarbonate buffer |
Ramachandran, J., and Sairam, M.R.: The Effects Of Interstitial Cell-Stimulating Hormone, Its Subunits,and Recombinants on Isolated Rat Leydig Cells, Arch Biochem Biophys
167,
294, 1975 |
|
|