Skin - Tissue Tables
Species | Species Detail | Cells | Enzyme(s) | Medium |
Reference |
Canine
|
Canine |
Epidermal |
Collagenase: 0.35%
Neutral Protease: 0.1%
|
DMEM |
Zheng, Y., Nace, A., Chen, W., Watkins, K., Sergott, L., Homan, Y., Vandeberg, J., Breen, M. and Stenn, K.: Mature Hair Follicles Generated from Dissociated Cells: A Universal Mechanism of Folliculoneogenesis., Dev Dyn
239,
2619-26, 2010 |
Frog
|
Frog |
Epidermal |
Trypsin: 0.18%
|
Barth's solution, CMF |
Nishikawa, A., Shimizu-Nishikawa, K., and Miler, L.: Isolation, characterization, and in vitro culture of larval and adult epidermal cells of the frog Xenopus laevis, In Vitro Cell Dev Biol 26,
1128, 1990 |
Goat
|
Goat |
Dermis isolated aggrecan sensitive |
Neutral Protease: 0.5%
Collagenase Type 2: 200 u/ml
|
DMEM |
Deng, Y., Hu, J. and Athanasiou, K.: Isolation and Chondroinduction of a Dermis-Isolated, Aggrecan-Sensitive Subpopulation with High Chondrogenic Potential., Arthritis Rheum 56,
168, 2007 |
Human
|
Human |
Diploid fibroblasts |
Collagenase Type 1: 0.03%
|
DMEM |
Tan, J., Jaafar, F. and Makpol, S.: Proteomic Profiling of Senescent Human Diploid Fibroblasts Treated with gamma-tocotrienol., BMC Complement Altern Med
18,
314, 2018 |
Human
|
Human |
Fibroblasts |
Neutral Protease: 0.1%
Collagenase Type 1: 0.25%
|
DMEM |
Chen, Z., Wang, X., Jin, T., Wang, Y., Hong, C., Tan, L., Dai, T., Wu, L., Zhuang, Z. and Shi, C.: Increase in the Radioresistance of Normal Skin Fibroblasts but not Tumor Cells by Mechanical Injury., Cell Death Dis
8,
e2573, 2017 |
Human
|
Human |
Mononuclear phagocytes |
Neutral Protease: 1 u/ml
Deoxyribonuclease I: 50 u/ml
Collagenase: 200 u/ml
Trypsin: 0.5%
|
RPMI-1640 |
Botting, Ra., Bertram, K., Baharlou, H., Sandgren, K., Fletcher, J., Rhodes, J., Rana, H., Plasto, T., Wang, X., Lim, J., Barnouti, L., Kohout, M., Papadopoulos, T., Merten, S., Olbourne, N. and Cunningham, A.: Phenotypic and Functional Consequences of Different Isolation Protocols on Skin Mononuclear Phagocytes., J Leukoc Biol 101,
1393-1403, 2017 |
Human
|
Human |
Skin |
Neutral Protease: 5 u/ml
Collagenase Type 3: 0.3%
Deoxyribonuclease I: 0.005%
|
RPMI 1640 |
Cheuk, S., Wiken, M., Blomqvist, L., Nylen, S., Talme, T., Stahle, M.and Eidsmo, L.: Epidermal Th22 and Tc17 Cells Form a Localized Disease Memory in Clinically Healed Psoriasis., J Immunol 192,
3111, 2014 |
Human
|
Human |
Skin fibroblasts |
Collagenase Type 1: 1000 u/ml
|
DMEM |
Douvaras, P., Wang, J., Zimmer, M., Hanchuk, S., O'Bara, M., Sadiq, S., Sim, F., Goldman, J. and Fossati, V.: Efficient Generation of Myelinating Oligodendrocytes from Primary Progressive Multiple Sclerosis Patients by Induced Pluripotent Stem Cells., Stem Cell Reports
3,
250, 2014 |
Human
|
Human |
Skin |
Collagenase Type 4: 0.08-0.2%
Neutral Protease: 0.1%
Deoxyribonuclease I: 0.005%
|
RPMI |
Cerny, D., Haniffa, M., Shin, A., Bigliardi, P., Tan, B., Lee, B., Poidinger, M., Tan, E., Ginhoux, F. and Fink, K.: Selective Susceptibility of Human Skin Antigen Presenting Cells to Productive Dengue Virus Infection., PLoS Pathog 10,
e1004548, 2014 |
Human
|
Human, adult |
Epidermal stem cells |
Neutral Protease: 2 u/ml
Trypsin: 2.5%
|
DMEM |
Nagel, S., Rohr, F., Weber, C., Kier, J., Siemers, F., Kruse, C., Danner, S., Brandenburger, M. and Matthiessen, A.: Multipotent Nestin-Positive Stem Cells Reside in the Stroma of Human Eccrine and Apocrine Sweat Glands and can be Propagated Robustly In Vitro., PLoS ONE
8,
e78365, 2013 |
Human
|
Human |
Fibroblasts |
Collagenase animal free: 2%
|
HBSS |
Karumbayaram, S., Lee, P., Azghadi, S., Cooper, A., Patterson, M., Kohn, D., Pyle, A., Clark, A., Byrne, J., Zack, J., Plath, K. and Lowry, W.: From Skin Biopsy to Neurons Through a Pluripotent Intermediate Under Good Manufacturing Practice Protocols, Stem Cells Transl Med 1,
36, 2012 |
Human
|
Human, female, 18-66 yr |
Fibroblasts |
Trypsin: 0.2%
|
DMEM |
Huschtscha, L., Napier, C., Noble, J., Bower, K., Au, A., Campbell, H., Braithwaite, A. and Reddel, R.: Enhanced Isolation of Fibroblasts From Human Skin Explants., Biotechniques
53,
239, 2012 |
Human
|
Human |
Fibroblasts and keratinocytes |
Neutral Protease: 0.5%
Collagenase Type 1: 0.2%
|
DMEM |
Carriel, V., Garzon, I., Jimenez, J., Oliveira, A., Arias-Santiago, S., Campos, A., Sanchez-Quevedo, M. and Alaminos, M.: Epithelial and Stromal Developmental Patterns in a Novel Substitute of the Human Skin Generated with Fibrin-Agarose Biomaterials., Cells Tissues Organs
196,
1-12, 2012 |
Human
|
Human |
Epidermal |
Collagenase: 0.35%
Neutral Protease: 0.1%
|
DMEM |
Zheng, Y., Nace, A., Chen, W., Watkins, K., Sergott, L., Homan, Y., Vandeberg, J., Breen, M. and Stenn, K.: Mature Hair Follicles Generated from Dissociated Cells: A Universal Mechanism of Folliculoneogenesis., Dev Dyn
239,
2619-26, 2010 |
Human
|
Human |
Skin and keloid progenitor |
Collagenase Type 1: 0.4%
Neutral Protease: 0.3%
|
PBS |
Zhang, Q., Yamaza, T., Kelly, AP, Shi, S., Wang, S., Brown, J., Wang, L., French, S., Shi, S. and Le, A.: Tumor-like Stem Cells Derived from Human Keloid are Governed by the Inflammatory Niche Driven by IL-17/IL-6 Axis., PLoS ONE
4,
e7798, 2009 |
Human
|
Human |
Fibroblasts |
Collagenase Type 1: 100-320 u/ml
|
DMEM |
Tuan, T., Hwu, P., Ho, W., Yiu, P., Chang, R., Wysocki, A. and Benya, P.: Adenoviral Overexpression and Small Interfering RNA Suppression Demonstrate that Plasminogen Activator Inhibitor-1 Produces Elevated Collagen Accumulation in Normal and Keloid Fibroblasts., Am J Pathol 173,
1311-25, 2008 |
Human
|
Human, 6-12 year |
Dermal fibroblasts |
Neutral Protease: 0.1%
Collagenase Type 1: 0.1%
|
DMEM |
Chen, F., Zhang, W., Bi, D., Liu, W., Wei, X., Chen, F., Zhu, L., Cui, L. and Cao, Y.: Clonal Analysis of Nestin(-) Vimentin(+) Multipotent Fibroblasts Isolated from Human Dermis., J Cell Sci
120,
2875, 2007 |
Human
|
Human |
Hair follicular epithelial |
Neutral Protease: 1.25%
Trypsin: 0.25%
|
DMEM/F-12 |
Yu Hong, Fang Dong, Kumar Suresh M, Li Ling, Nguyen Thiennga K, Acs Geza, Herlyn Meenhard, Xu Xiaowei: Isolation of a novel population of multipotent adult stem cells from human hair follicles, Am J Pathol 168,
1879-88, 2006 |
Human
|
Human |
fibroblasts |
Collagenase: 0.5%
Trypsin: 0.25%
|
DMEM/F12 |
Clark, R., Chong, B., Mirchandani, N., Yamanaka, K., Murphy, G., Dowgiert, R. and Kupper, T.: A Novel Method for the Isolation of Skin Resident T cells from Normal and Diseased Human Skin., J Invest Dermatol 126,
1059, 2006 |
Human
|
Human, neonatal |
Keratinocytes |
Neutral Protease: 0.4%
Collagenase: 0.3%
|
DMEM |
Li, A., Pouliot, N., Redvers, R., and Kaur, P.: Extensive tissue-regenerative capacity of neonatal human keratinocytes stem cells and their progeny, J Clin Invest 113,
390-400, 2004 |
Human
|
Human |
Mast cells |
Neutral Protease: 0.05%
Collagenase Type 4: 1.0%
|
RPMI |
Babina Magda, Guhl Sven, Starke Andrae, Kirchhof Loreen, Zuberbier Torsten, Henz Beate M: Comparative cytokine profile of human skin mast cells from two compartments--strong resemblance with monocytes at baseline but induction of IL-5 by IL-4 priming, J Leukoc Biol 75,
244-52, 2004 |
Human
|
Human, adult |
Dermal fibroblasts |
Collagenase: 0.5-1.0%
|
DMEM |
Wang, H., Van Blitterswijk, C., Bertrand-De Haas, M., Schuurman, A. and Lamme, E.: Improved Enzymatic Isolation of Fibroblasts for the Creation of Autologous Skin Substitutes., In Vitro Cell Dev Biol Anim 40,
268, 2004 |
Human
|
Human |
Fibroblasts |
Trypsin: 0.25%
|
DMEM |
Tuan Tai-Lan, Wu Huayang, Huang Eunice Y, Chong Sheree S N, Laug Walter, Messadi Diana, Kelly Paul, Le Anh: Increased plasminogen activator inhibitor-1 in keloid fibroblasts may account for their elevated collagen accumulation in fibrin gel cultures, Am J Pathol 162,
1579-89, 2003 |
Human
|
Human |
Keratinocytes, fibroblasts, endothelial |
Neutral Protease: see reference
Collagenase: see reference
|
see reference |
Supp Dorothy M, Wilson-Landy Kaila, Boyce Steven T: Human dermal microvascular endothelial cells form vascular analogs in cultured skin substitutes after grafting to athymic mice, FASEB J
16,
797-804, 2002 |
Human
|
Human, adult |
Keratinocytes |
Neutral Protease: 0.25%
|
PBS |
Baudoux, B., Castanares-Zapatero, D., Leclercq-Smekens, M., Berna, N., and Poumay, Y.: The Tetraspanin CD9 Associates with the Integrin a6B4 in Cultured Human Epidermal Keratinocytes and is Involved in Cell Motility, Eur J Cell Biol
79,
41, 2000 |
Human
|
Human |
Keratinocytes |
Neutral Protease: 0.25%
Trypsin: 0.17%
Thermolysin: 0.05%
|
see reference |
Hybbinette S, Bostrom M, Lindberg K: Enzymatic dissociation of keratinocytes from human skin biopsies for in vitro cell propagation, Exp Dermatol
8,
30-8, 1999 |
Human
|
Human |
Human skin mast cells |
Neutral Protease: 0.1%
Collagenase Type 1: see reference
|
PBS |
Grutzkau A, Kruger-Krasagakes S, Baumeister H, Schwarz C, Kogel H, Welker P, Lippert U, Henz BM, Moller A: Synthesis, storage, and release of vascular endothelial growth factor/vascular permeability factor (VEGF/VPF) by human mast cells: implications for the biological significance of VEGF206, Mol Biol Cell
9,
875-84, 1998 |
Human
|
Human |
Epidermal |
Collagenase Type 2: 0.1%
Neutral Protease: 0.5-1.0%
|
PBS |
Reece J C, Handley A J, Anstee E J, Morrison W A, Crowe S M, Cameron P U: HIV-1 selection by epidermal dendritic cells during transmission across human skin, J Exp Med 187,
1623-31, 1998 |
Human
|
Human |
Keratinocytes |
Trypsin: 0.05%
|
Dulbecco's PBS |
Judd, D., Battista, P., and Behm, D.: Culture of Human Keratinocytes in Defined Serum-Free Medium, Focus 19 (1),
2, 1997 |
Human
|
Human |
Epidermis plus dermis Abdomen or inner forearm |
Trypsin: 0.125%
|
DMEM, M199 |
Harley, C., and Sherwood, S.: Methods in Molecular Biology, Basic Cell Culture Protocols, 2nd ed. Vol. 75,Pollard, J., and Walker, J.,
Humana Press,
23, 1997 |
Human
|
Human |
Keratinocytes |
Trypsin: 0.25%
|
DMEM |
Regnier, M: Culture of Human Karatinocytes,Cell & Tissue Culture: Laboratory Procedures Vol. 1,Doyle, A., Griffiths, J., and Newell, D.,
John Wiley and Sons Ltd,
11B:4.1, 1995 |
Human
|
Human, adult, female, breast skin |
Keratinocytes |
Trypsin: 0.25%
|
PBS |
Hirel, B., Chesne, C., Pailgheret, J., and Guillouzo, A.: Expression of Differentiation Markers in Human Adult Keratinocytes Cultured in Submerged Conditions, In Vitro Cell Dev Biol 30A,
372, 1994 |
Human
|
Human, neonatal foreskin |
Fibroblasts |
Trypsin: 0.25%
|
DMEM |
Hansbrough, J., Cooper, M., Cohen, R., Spielvogel, R., Greenleaf, G., Bartel, R., and Naughton, G.: Evaluation of a Biodegradable Matrix Containing Cultured Human Fibroblasts as a Dermal Replacement Beneath Meshed Skin Grafts on Athymic Mice, Surgery 111 (1),
438, 1992 |
Human
|
Human, 18-50 years |
Sweat duct |
Collagenase: 0.03%
|
MEM |
Bell, C. and Quinton, P.: Effects of Media Buffer Systems on Growth and Electrophysiologic Characteristics of Cultured Sweat Duct Cells, In Vitro Cell Dev Biol 27,
47, 1991 |
Human
|
Human |
Fibroblasts |
Trypsin: 0.25%
|
CMF solution |
Limat, A., Hunziker, T., Boillat, C., Noser, F., and Wiesmann, U.: Postmitotic Human Dermal Fibroblasts Preserve Intact Feeder Properties for Epithelial Cell Growth After Long-Term Cryopreservation, In Vitro Cell Dev Biol 26,
709, 1990 |
Human
|
Human |
Sweat gland |
Collagenase Type 2: 0.015%
|
(see reference) |
Krouse, M., Hagiqara, G., Chen, J., Lewiston, N., and Wine, J.: Ion Channels in Normal Human and Cystic Fibrosis Sweat Gland Cells, Am J Physiol 257,
C129, 1989 |
Human
|
Human, ages 16-30 |
Human sweat duct |
Collagenase Type 2: 0.2%
|
RPMI 1640 (see reference) |
Pedersen, P.: Human Sweat Duct Cells in Primary Culture. Basic Bioelectric Properties of Cultures Derived From Normals and Patients with Cystic Fibrosis, In Vitro Cell Dev Biol 25 (4),
342, 1989 |
Human
|
Human |
Melanocytes, skin/ foreskin |
Trypsin: 0.25%
|
PBS |
Peacocke, M., Yaar, M., Mansur, C., Chao, M., and Gilchrest, B.: Induction of Nerve Growth Factor Receptors on Cultured Human Melanocytes, Proc Natl Acad Sci U S A 85,
5282, 1988 |
Human
|
Human |
Human sweat glands |
Collagenase: 0.2%
|
HBSS |
Lee, C., Carpenter, F., Coaker, T., and Kealey, T.: The Primary Culture of Epithelia From the Secretory Coil and Collecting Duct of Normal Human and Cystic Fibrotic Eccrine Sweat Glands, J Cell Sci
83,
103, 1986 |
Human
|
Human |
Keratinocytes |
Trypsin: 0.25%
|
DMEM |
Dover, R. and Potten, C.: Cell Cycle Kinetics of Cultured Human Epidermal Keratinocytes, J Invest Dermatol 80,
423, 1983 |
Human
|
Human, adult |
Epidermal keratinocytes |
Trypsin: 0.25%
Collagenase: 0.2%
Deoxyribonuclease I: 0.001%
|
Eagle's MEM |
Alitalo K, Kuismanen E, Myllyla R, Kiistala U, Asko-Seljavaara S, Vaheri A: Extracellular matrix proteins of human epidermal keratinocytes and feeder 3T3 cells, J Cell Biol
94,
497-505, 1982 |
Human
|
Human, fetal |
Smooth muscle, fibroblasts |
Trypsin: 0.055%
|
DMEM |
Davies, P. and Kerr, C.: Modification of LDL Metabolism by Growth Factors in Cultured Vascular Cells and Human Skin Fibroblasts, Biochim Biophys Acta 712,
26, 1982 |
Human
|
Human, 18-30 years, male, female |
Fibroblasts |
Trypsin: 0.1%
|
HBSS |
McCoy, B., Galdun, J., and Cohen, I.: Effects of Density and Cellular Aging On Collagen Synthesis and Growth Kinetics in Keloid and Normal Skin Fibroblasts, In Vitro 18 (1),
79, 1982 |
Human
|
Human, newborn |
Keratinocytes |
Trypsin: 0.3%
|
DMEM |
Liu, S., Eaton, M., and Karasek, M.: Growth Characteristics of Human Epidermal Keratinocytes from Newborn Foreskin in Primary and Serial Cultures, In Vitro 15 (10),
813, 1979 |
Human
|
Human |
Keratinocytes |
Trypsin: 0.25%
|
(see reference) |
Rheinwald, J., and Green, H.: Serial Cultivation of Strains of Human Epidermal Kerotinocytes: The Formation of Keratinizing Colonies from Single Cells, Cell
6,
331, 1975 |
Mouse
|
Mouse |
Keratinocytes |
Collagenase Type 4: 0.2%
Deoxyribonuclease I: 0.005%
Neutral Protease: 5 u/ml
|
DMEM |
Spidale, N., Malhotra, N., Frascoli, M., Sylvia, K., Miu, B., Freeman, C., Stadinski, B., Huseby, E. and Kang, J.: Neonatal-Derived IL-17 Producing Dermal gd T Cells are Required to Prevent
Spontaneous Atopic Dermatitis, Elife
9,
5118, 2020 |
Mouse
|
Mouse |
Keratinocytes |
Trypsin: 0.25%
Collagenase Type 1: 0.12%
Collagenase Type 2: 0.05%
Collagenase Type 4: 0.05%
Hyaluronidase: 0.1%
|
Low calcium containing media |
Blanco, S., Bandiera, R., Popis, M., Hussain, S., Lombard, P., Aleksic, J., Sajini, A., Tanna, H., Cortes-Garrido, R., Gkatza, N., Dietmann, S. and Frye, M.: Stem Cell Function and Stress Response are Controlled by Protein Synthesis., Nature
534,
335-40, 2016 |
Mouse
|
Mouse |
Dermal fibroblasts |
Elastase: 0.012%
Collagenase Type 4: 0.4%
|
DMEM |
Rinkevich, Y., Walmsley, G., Hu, M., Maan, Z., Newman, A., Drukker, M., Januszyk, M., Krampitz, G., Gurtner, G., Lorenz, H., Weissman, I. and Longaker, M.: Identification and Isolation of a Dermal Lineage with Intrinsic Fibrogenic Potential., Science 348,
aaa2151, 2015 |
Mouse
|
Mouse |
Skin |
Collagenase Type 4: 0.08-0.2%
Neutral Protease: 0.1%
Deoxyribonuclease I: 0.005%
|
RPMI |
Cerny, D., Haniffa, M., Shin, A., Bigliardi, P., Tan, B., Lee, B., Poidinger, M., Tan, E., Ginhoux, F. and Fink, K.: Selective Susceptibility of Human Skin Antigen Presenting Cells to Productive Dengue Virus Infection., PLoS Pathog 10,
e1004548, 2014 |
Mouse
|
Mouse |
Epidermal |
Neutral Protease: 0.5%
Collagenase Type 4: 0.1%
|
DMEM |
Gu, D., Fan, Q and Xie, J.: Cell Population Analyses During Skin Carcinogenesis., J Vis Exp
,
e50311, 2013 |
Mouse
|
Mouse |
Epithelial stem |
Trypsin: 0.25%
Collagenase Type 1: 0.4%
|
HBSS |
Jensen, U., Ghazizadeh, S. and Owens, D.: Isolation and Characterization of Cutaneous Epithelial Stem Cells., Methods Mol Biol 989,
61-9, 2013 |
Mouse
|
Mouse |
Mast |
Collagenase Type 4: 0.2%
|
IMDM |
Antsiferova, M., Martin, C., Huber, M., Feyerabend, T., Forster, A., Hartmann, K., Rodewald, H., Hohl, D. and Werner, S.: Mast Cells are Dispensable for Normal and Activin-Promoted Wound Healing and Skin Carcinogenesis., J Immunol 191,
6147-55, 2013 |
Mouse
|
Mouse |
Dermal fibroblasts |
Collagenase: 400 u/ml
|
AminoMAX II |
Scheibye-Knudsen, M., Ramamoorthy, M., Sykora, P., Maynard, S., Lin, P., Minor, R., Wilson, D., Cooper, M., Spencer, R., de Cabo, R., Croteau, D. and Bohr, V.: Cockayne Syndrome Group B Protein Prevents the Accumulation of Damaged Mitochondria by Promoting Mitochondrial Autophagy., J Exp Med 209,
855, 2012 |
Mouse
|
Mouse |
Epidermal and dermal |
Collagenase Type 4: 0.18%
Collagenase/Dispase: 0.18%
|
PBS |
King, I., Kroenke, M. and Segal, B.: GM-CSF-Dependent, CD103+ Dermal Dendritic Cells Play a Critical Role in Th Effector Cell Differentiation After Subcutaneous Immunization., J Exp Med 207,
953, 2010 |
Mouse
|
Mouse, 2-3 day |
Dermal fibroblasts |
Collagenase Type 2: 0.04%
Trypsin: 0.025%
|
DME |
Farina, G., York, M., Di Marzio, M., Collins, C., Meller, S., Homey, B., Rifkin, I., Marshak-Rothstein, A., Radstake, T. and Lafyatis, R.: Poly(I:C) Drives Type I IFN- and TGFβ-Mediated Inflammation and Dermal Fibrosis Simulating Altered Gene Expression in Systemic Sclerosis., J Invest Dermatol 130,
2583-93, 2010 |
Mouse
|
Mouse |
Dermal |
Collagenase Type 3: 0.3%
Deoxyribonuclease I: 0.0005%
|
HBSS |
Eidsmo, L., Allan, R., Caminschi, I., Van Rooijen, N., Heath, W. and Carbone, F.: Differential Migration of Epidermal and Dermal Dendritic Cells During Skin Infection., J Immunol 182,
3165, 2009 |
Mouse
|
Mouse, neonatal |
Dermal |
Collagenase: 0.35%
Deoxyribonuclease I: see reference
|
PBS |
Crigler Lauren, Kazhanie Amita, Yoon Tae-Jin, Zakhari Julia, Anders Joanna, Taylor Barbara, Virador VictoriaM: Isolation of a mesenchymal cell population from murine dermis that contains progenitors of multiple cell lineages, FASEB J
21,
2050-63, 2007 |
Mouse
|
mouse |
Dermal fibroblasts |
Trypsin: 0.25%
Collagenase Type 1: 0.25%
|
DMEM |
Baxter Ruth M, Crowell Thomas P, McCrann Margaret E, Frew Erica M, Gardner Humphrey: Analysis of the tight skin (Tsk1/+) mouse as a model for testing antifibrotic agents, Lab Invest 85,
1199-209, 2005 |
Mouse
|
Mouse, neonatal |
Microvascular endothelial |
Neutral Protease: 0.005%
Collagenase Type 1: 4%
|
DMEM |
Cha, S., Talavera, D., Demir, E., Nath, A. and Sierra-Honigmann, M.: A Method of Isolation and Culture of Microvascular Endothelial Cells from Mouse Skin., Microvasc Res 70,
198, 2005 |
Mouse
|
Mouse, male 6-24 week |
Skin side population |
Collagenase Type 4: 0.2%
Neutral Protease: 1.2 u/ml
|
PBS |
Montanaro F, Liadaki K, Volinski J, Flint A, and Kunkel LM.: Skeletal muscle engraftment potential of adult mouse skin side population cells, Proc Natl Acad Sci U S A 100,
9336, 2003 |
Mouse
|
Mouse |
Ear epidermal |
Trypsin: 0.1%
Collagenase: 0.2%
|
PBS |
Takanami-Ohnishi Yoko, Amano Shinya, Kimura Sadao, Asada Sachie, Utani Atsushi, Maruyama Masumi, Osada Hiroyuki, Tsunoda Hajime, Irukayama-Tomobe Yoko, Goto Katsutoshi, Karin Michael, Sudo Tatsuhiko, Kasuya Yoshitoshi: Essential role of p38 mitogen-activated protein kinase in contact hypersensitivity, J Biol Chem 277,
37896-903, 2002 |
Mouse
|
Mouse, 3-7 month |
Fibroblasts, mesangial, smooth muscle |
Trypsin: 0.25%
Collagenase: see reference
Soybean Trypsin Inhibitor: 0.05%
|
DMEM |
Bradshaw AD, Francki A, Motamed K, Howe C, Sage EH: Primary mesenchymal cells isolated from SPARC-null mice exhibit altered morphology and rates of proliferation, Mol Biol Cell
10,
1569-79, 1999 |
Porcine
|
Porcine, 3-4 month |
Synovial membrane and skin stem |
Collagenase: 0.2%
|
DMEM |
Ando, W., Kutcher, J., Krawetz, R., Sen, A., Nakamura, N., Frank, C. and Hart, D.: Clonal Analysis of Synovial Fluid Stem Cells to Characterize and Identify Stable Mesenchymal Stromal Cell/Mesenchymal Progenitor Cell Phenotypes in a Porcine Model: A Cell Source with Enhanced Commitment to the Chondrogenic Lineage., Cytotherapy
16,
776, 2014 |
Porcine
|
Porcine, 2-6 month |
Keratinocytes |
Neutral Protease: 0.25%
|
Dulbecco-Vogt MEM |
Regauer S, Compton C: Cultured porcine epithelial grafts: an improved method, J Invest Dermatol 94,
230-4, 1990 |
Rat
|
Rat, Wistar, 4-8 day |
Dermal fibroblasts and keratinocytes |
Trypsin: 0.25%
|
Ham's F-12 |
Sugihara, H and Toda, S: Primary Tissue Intact and Dissociated Cell Culture,Cell & Tissue Culture: Laboratory Procedures Vol. 1,Doyle, A., Griffiths, J., and Newell, D.,
John Wiley and Sons, Ltd.,
3A:2.1, 1995 |
Rat
|
Rat, SD, male, 40-60 days |
Sebaceous |
Trypsin: 0.2%
|
DMEM with FBS PBS |
Laurent, S., Mednieks, M., and Rosenfield, R.: Growth of Sebaceous Cells in Monolayer Culture, In Vitro Cell Dev Biol 28,
83, 1992 |
Rat
|
Rat, neonatal, 12-24 hr post partum |
Fibroblasts |
Trypsin: 0.2%
|
HEPES buffered DMEM |
Acheson, A., Barker, P., Alderson, R., Miller, F., and Murphy, R.: Detection of Brain-Derived Neurotrophic Factor-like Activity in Fibroblasts and Schwann Cells: Inhibition by Antibodies to NGF, Neuron 7,
265, 1991 |
Rat
|
Rat, albino, one day old, CFN |
Keratinocytes |
Trypsin: 1%
|
EBSS |
Vaughan, F., Gray, R., and Bernstein, I.: Growth and Differentiation of Primary Rat Keratinocytes on Synthetic Membranes, In Vitro Cell Dev Biol 22 (3),
141, 1986 |
|
|