Spleen - Tissue Tables
Species | Species Detail | Cells | Enzyme(s) | Medium |
Reference |
Mouse
|
Mouse, 4 week |
Trophoblast |
Collagenase Type 1: 0.1%
Collagenase Type 2: 0.25%
|
DMEM |
Hou, M., Han, J., Li, G., Kwon, M., Jiang, J., Emani, S., Taglauer, E., Park, J., Choi, E., Vodnala, M., Fong, Y., Emani, S., Rosas, I., Perrella, M. and Liu, X.: Multipotency of Mouse Trophoblast Stem Cells, Stem Cell Res Ther 11,
55, 2020 |
Mouse
|
Mouse |
Speen |
Collagenase Type 5: 5%
Hyaluronidase: 2.5%
Elastase: 1.25%
|
HBSS |
Villarreal, E., Hewgley, W., Lang, W., Morton, C., Mao, S., Wu, J. and Sandoval, J.: In-bag Enzymatic Splenic Digestion: A Novel Alternative to Manual Morcellation?, J Surg Res 218,
209-216, 2017 |
Mouse
|
Mouse |
Splenic |
Collagenase Type 2: 0.1%
|
PBS |
Klebanoff, C., Spencer, S., Torabi-Parizi, P., Grainger, J., Roychoudhuri, R., Ji, Y., Sukumar, M., Muranski, P., Scott, C., Hall, J., Ferreyra, G., Leonardi, A., Borman, Z. et al.: Retinoic Acid Controls the Homeostasis of Pre-cDC-Derived Splenic and Intestinal Dendritic Cells., J Exp Med 210,
1961, 2013 |
Mouse
|
Mouse |
Splenocytes |
Collagenase Type 2: 0.16%
Deoxyribonuclease I: 0.002%
|
PBS |
Yi, T. and Cyster, J.: EBI2-Mediated Bridging Channel Positioning Supports Splenic Dendritic Cell Homeostasis and Particulate Antigen Capture., Elife
2,
e00757, 2013 |
Mouse
|
Mouse |
Spleen, bone marrow endothelial |
Collagenase Type 4: 0.3-1.0%
Deoxyribonuclease I: 20 u/ml
|
PBS |
Shi, C., Jia, T., Mendez-Ferrer, S., Hohl, T., Serbina, N., Lipuma, L., Leiner, I., Li, M., Frenette, P. and Pamer, E.: Bone Marrow Mesenchymal Stem and Progenitor Cells Induce Monocyte Emigration in Response to Circulating Toll-Like Receptor Ligands., Immunity
34,
590, 2011 |
Mouse
|
Mouse, female, 6-8 week |
Dendritic |
Collagenase Type 4: 0.05%
|
RPMI 1640 |
Abou Fakher, F., Rachinel, N., Klimczak, M., Louis, J. and Doyen, N.: TLR9-Dependent Activation of Dendritic Cells by DNA from Leishmania Major Favors Th1 Cell Development and the Resolution of Lesions., J Immunol 182,
1386, 2009 |
Mouse
|
Mouse |
Dendritic |
Collagenase Type 1: 0.5%
|
HBSS |
Flano, E., Jewell, N., Durbin, R. and Durbin, J.: Methods Used to Study Respiratory Virus Infection.,Curr Protoc Cell Biol Vol. Chapter 26,,
,
Unit 26.3, 2009 |
Mouse
|
Mouse |
Dentritic |
Collagenase: 300 u/ml
Deoxyribonuclease I: 0.002%
|
RPMI 1640 |
Abe, K., Nguyen, K., Fine, S., Mo, J., Shen, C., Shenouda, S., Corr, M., Jung, S., Lee, J., Eckmann, L. and Raz, E.: Conventional Dendritic Cells Regulate the Outcome of Colonic Inflammation Independently of T Cells., Proc Natl Acad Sci U S A 104,
17022, 2007 |
Mouse
|
Mouse |
Splenic |
Collagenase Type 2: 0.1%
Deoxyribonuclease I: 0.1%
|
RPMI |
Mueller, S., Matloubian, M., Clemens, D, Sharpe, A., Freeman, G., Gangappa, S., Larsen, C. and Ahmed, R.: Viral Targeting of Fibroblastic Reticular Cells Contributes to Immunosuppression and Persistence During Chronic Infection., Proc Natl Acad Sci U S A 104,
15430, 2007 |
Mouse
|
Mouse |
Splenic dendritic |
Collagenase: 0.1%
Deoxyribonuclease I: 0.005%
|
DMEM |
Kang, H., Liu, M. and Datta, S.: Low-dose Peptide Tolerance Therapy of Lupus Generates Plasmacytoid Dendritic Cells That Cause Expansion of Autoantigen-Specific Regulatory T Cells and Contraction of Inflammatory Th17 Cells, J Immunol 178,
7849-58, 2007 |
Mouse
|
Mouse |
Splenic stromal |
Collagenase Type 3: 100-400 u/ml
|
HBSS |
Benedict Chris A, De Trez Carl, Schneider Kirsten, Ha Sukwon, Patterson Ginelle, Ware Carl F: Specific remodeling of splenic architecture by cytomegalovirus, PLoS Pathog 2,
e16, 2006 |
Mouse
|
Mouse |
Leukocytes |
Collagenase Type 4: 43 u/ml
|
RPMI 1640 |
Siragam, V., Crow, A., Brinc, D., Song, S., Freedman, J. and Lazarus, A.: Intravenous Immunoglobulin Ameliorates ITP via Activating Fc Gamma Receptors on Dendritic Cells., Nat Med 12,
688, 2006 |
Mouse
|
Mouse, 4-6 week |
Dentritic |
Collagenase Type 1: 0.1%
Deoxyribonuclease I: 0.001%
|
RPMI-1640 |
McLellan, A., Kapp, M., Eggert, A., Linden, C., Bommhardt, U., Brocker, E., Kammerer, U. and Kampgen, E.: Anatomic Location and T-cell Stimulatory Functions of Mouse Dendritic Cell Subsets Defined by CD4 and CD8 Expression, Blood 99,
2084-93, 2002 |
Mouse
|
Mouse |
Dentritic |
Collagenase Type 3: 0.1%
Deoxyribonuclease I: 325 u/ml
|
RPMI 1640 |
Schiavoni, F, Mattei, F, Sestili, P, Borghi, P, Venditti, M, Morse, H, Belardelli, F, and Gabrieli, L: ICSBP is Essential for the Development of Mouse Type I Interferon-producing Cells and for the Generation and Activation of CD8a+ Dendritic Cells, J Exp Med 196,
1415, 2002 |
Mouse
|
Mouse |
Dentritic cells |
Collagenase Type 4: 0.1%
Deoxyribonuclease I: 20 ug/ml
|
HEPES/RPMI 1640 |
Stagg, AJ, Burke, F, Hill, S and Knight, S.: Isolation of Mouse Spleen Dendritic Cells., Methods Mol Med
64,
9-22, 2001 |
Mouse
|
Mouse |
Dentritic |
Collagenase: 100 u/ml
|
HBSS |
Brasel K, De Smedt T, Smith JL, Maliszewski CR: Generation of murine dendritic cells from flt3-ligand-supplemented bone marrow cultures, Blood 96,
3029-39, 2000 |
|
|