Stem - Tissue Tables
Species | Species Detail | Cells | Enzyme(s) | Medium |
Reference |
Canine
|
23-33 kg |
Stromal vascular |
Collagenase: see reference
|
Media 199E |
Williams, S., Kosnik, P., Kleinert, L., Vossman, E., Lye, K. and Shine, M.: Adipose Stromal Vascular Fraction Cells Isolated Using an Automated Point of Care System Improve the Patency of Expanded Polytetrafluoroethylene Vascular Grafts., Tissue Eng Part A
19,
1295-302, 2013 |
Canine
|
Canine |
Amniotic membrane MSC |
Trypsin: 0.25%
Collagenase Type 1: 0.2%
|
LG-DMEM |
Park, S., Seo, M., Kim, H. and Kang, K.: Isolation and Characterization of Canine Amniotic Membrane-Derived Multipotent Stem Cells., PLoS ONE
7,
e44693, 2012 |
Canine
|
Canine, 20-25 kg |
Adipose stem cells |
Collagenase: see reference
|
Media-199 |
Fischer, L., McIlhenny, S., Tulenko, T., Golesorkhi, N., Zhang, P., Larson, R., Lombardi, J., Shapiro, I. and DiMuzio, P.: Endothelial Differentiation of Adipose-Derived Stem Cells: Effects of Endothelial Cell Growth Supplement and Shear Force., J Surg Res 152,
157, 2009 |
Equine
|
Equine |
Adipose derived stem cells |
Collagenase Type 1: 0.1%
|
PBS |
Vidal, M., Robinson, S., Lopez, M., Paulsen, D., Borkhsenious, O., Johnson, J., Moore, R. and Gimble, J.: Comparison of Chondrogenic Potential in Equine Mesenchymal Stromal Cells Derived from Adipose Tissue and Bone Marrow., Vet Surg
37,
713, 2008 |
Equine
|
Equine, 1-5 year |
Adipose derived stem cells |
Collagenase Type 1: 0.1%
|
PBS |
Vidal, M., Kilroy, G., Lopez, M., Johnson, J., Moore, R. and Gimble, J.: Characterization of Equine Adipose Tissue-Derived Stromal Cells: Adipogenic and Osteogenic Capacity and Comparison with Bone Marrow-Derived Mesenchymal Stromal Cells., Vet Surg
36,
613, 2007 |
Human
|
Human |
Adipose derived stem |
Collagenase Type 4: 150-300 u/ml
|
RPMI-1640 |
Alstrup, T., Eijken, M., Bohn, A., Moller, B. and Damsgaard, T.: Isolation of Adipose Tissue-Derived Stem Cells: Enzymatic Digestion in Combination with Mechanical Distortion to Increase Adipose Tissue-Derived Stem Cell Yield from Human Aspirated Fat., Curr Protoc Stem Cell Biol
48,
e68, 2019 |
Human
|
Human |
Postnatal periodontal ligament |
Collagenase Type 1: 0.01%
|
DMEM/F12 |
Yam, G., Teo, E., Setiawan, M., Lovatt, M., Yusoff, N., Fuest, M., Goh, B. and Mehta, J.: Postnatal Periodontal Ligament as a Novel Adult Stem Cell Source for Regenerative Corneal Cell Therapy., J Cell Mol Med
,
, 2018 |
Human
|
Human |
Wharton jelly mesenchymal stromal |
Collagenase Type 2: 270 u/ml
|
DMEM/F12 |
Oppliger, B., Joerger-Messerli, M., Simillion, C., Mueller, M., Surbek, D. and Schoeberlein, A.: Mesenchymal Stromal Cells from Umbilical Cord Wharton's Jelly Trigger Oligodendroglial Differentiation in Neural Progenitor Cells Through Cell-to-Cell Contact., Cytotherapy
19,
829-838, 2017 |
Human
|
Human |
Umbilical cord mesenchymal stromal |
Collagenase Type 1: 0.2%
Hyaluronidase: 0.1%
Deoxyribonuclease I: 0.01%
|
MEM |
de Witte, S., Lambert, E., Merino, A., Strini, T., Douben, H., O'Flynn, L., Elliman, S., de Klein, A., Newsome, P., Baan, C. and Hoogduijn, M.: Aging of Bone Marrow- and Umbilical Cord-Derived Mesenchymal Stromal Cells During Expansion., Cytotherapy
19,
798-807, 2017 |
Human
|
Human |
Synovial mesenchymal stem |
Collagenase: 0.3%
|
MEM |
Matsumura, E., Tsuji, K., Komori, K., Koga, H., Sekiya, I., Muneta, T.: Pretreatment with IL-1β Enhances Proliferation and Chondrogenic Potential of Synovium-Derived Mesenchymal Stem Cells., Cytotherapy
19,
181-193, 2017 |
Human
|
Human |
Adipose derived mesenchymal stem |
Collagenase animal free: 0.1%
|
PBS |
Clark, K., Fierro, F., Ko, E., Walker, N., Arzi, B., Tepper, C., Dahlenburg, H., Cicchetto, A., Koi, A., Marsh, L., Murphy, W., Fazel, N., and Borjesson, D.: Human and Feline Adipose-Derived Mesenchymal Stem Cells Have Comparable Phenotype, Immunomodulatory Functions, and Transcriptome., Stem Cell Res Ther 8,
69, 2017 |
Human
|
Human |
Motor neurons |
Papain: 20 u/ml
|
EBSS |
Rigamonti, A., Repetti, G., Sun, C., Price, F., Reny, D., Rapino, F., Weisinger, K., Benkler, C., Peterson, Q., Davidow, L., Hansson, E. and Rubin, L.: Large-Scale Production of Mature Neurons from Human Pluripotent Stem Cells in a Three-Dimensional Suspension Culture System., Stem Cell Reports
6,
993-1008, 2016 |
Human
|
Human |
Wharton jelly mesenchymal stromal |
Collagenase Type 2: 0.6%
|
DMEM |
Lim, J., Razi, Z., Law, J., Nawi, A., Idrus, R. and Ng, M.: MSCs can be Differentially Isolated From Maternal, Middle and Fetal Segments of the Human Umbilical Cord., Cytotherapy
18,
1493-1502, 2016 |
Human
|
Human |
Adipose stem |
Collagenase Type 1: 0.1%
|
DMEM/F12 |
Cheng, N., Hsieh, T., Lai, H. and Young, T.: High Glucose-Induced Reactive Oxygen Species Generation Promotes Stemness in Human Adipose-Derived Stem Cells, Cytotherapy
18,
371-83, 2016 |
Human
|
Human |
Tendon stem |
Collagenase Type 1: 0.3%
Neutral Protease: 0.4%
|
MEM |
Randelli, P., Menon, A., Ragone, V., Creo P., Alfieri, M., Perucca, O., Banfi, G., Cabitza, P., Tettamanti, G. and Anastasia, L.: Effects of the Pulsed Electromagnetic Field PST on Human Tendon Stem Cells: a Controlled Laboratory Study., BMC Complement Altern Med
16,
293, 2016 |
Human
|
Human |
Mesenchymal stromal |
Collagenase Type 4: 0.2%
|
PBS |
Choudhery,M, Badowski, M, Muise, A and Harris, D: Effect of Mild Heat Stress on the Proliferative and Differentiative Ability of Human Mesenchymal Stromal Cells., Cytotherapy
17,
359-68, 2015 |
Human
|
Human |
Adipose derived stem cells |
Collagenase Type 2: 0.1%
|
HBSS |
Satish, L., Krill-Burger, J., Gallo, P., Etages, S., Liu, F., Philips, B., Ravuri, S., Marra, K., LaFramboise, W., Kathju, S. and Rubin, J.: Expression Analysis of Human Adipose-Derived Stem Cells During In Vitro Differentiation to an Adipocyte Lineage., BMC Med Genomics
8,
41, 2015 |
Human
|
Human |
Dental mesenchymal stem |
Collagenase Type 1: 0.3%
Neutral Protease: 0.4%
|
DMEM |
Wu, Z., Wang, J., Dong, R., Wang, L., Fan, Z., Liu, D. and Wang, S.: Depletion of MEIS2 Inhibits Osteogenic Differentiation Potential of Human Dental Stem Cells., Int J Clin Exp Med
8,
7220-30, 2015 |
Human
|
Human |
Decidua mesenchymal stem |
Trypsin: 0.25%
Collagenase Type 1: 1.0%
Deoxyribonuclease I: 0.005%
|
PBS |
Kusuma, G., Menicanin, D., Gronthos, S., Manuelpillai, U., Abumaree, M., Pertile, M., Brennecke, S. and Kalionis, B.: Ectopic Bone Formation by Mesenchymal Stem Cells Derived from Human Term Placenta and the Decidua., PLoS ONE
10,
e0141246, 2015 |
Human
|
Human |
Skeletal muscle stem |
Collagenase Type 2: 750 u/ml
Neutral Protease: 2 u/ml
|
Ham's F10 |
Charville, G., Cheung, T., Yoo, B., Santos, P., Lee, G., Shrager, J. and Rando, T.: Ex Vivo Expansion and In Vivo Self-Renewal of Human Muscle Stem Cells., Stem Cell Reports
5,
621, 2015 |
Human
|
Human |
Spinal cord neural progenitor |
PDS kit: with modifications
|
EBSS |
Mothe, A. and Tator, C.: Isolation of Neural Stem/Progenitor Cells from the Periventricular Region of the Adult Rat and Human Spinal Cord., J Vis Exp
,
e52732, 2015 |
Human
|
Human |
Wharton jelly mesenchymal stem |
Collagenase Type 2: 0.1%
Trypsin NF 1:250: 0.25%
|
PBS |
Lee, S., Park, B., Kim, J., Jekarl, D., Choi, H., Lee, S., Kim, M., Kim, Y. and Park, M.: The Effect of Fibroblast Growth Factor on Distinct Differentiation Potential of Cord Blood-Derived Unrestricted Somatic stem Cells and Wharton's Jelly-Derived Mesenchymal Stem/Stromal Cells., Cytotherapy
17,
1723-31, 2015 |
Human
|
Human, adult |
Adipose derived mesenchymal stem |
Collagenase Type 2: 0.1%
|
see reference |
Al-Saqi, S., Saliem, M., Asikainen, S., Quezada, H., Ekblad, A., Hovatta, O., Le Blanc, K., Jonasson, A. and Gotherstrom, C.: Defined Serum-Free Media for In Vitro Expansion of Adipose-Derived Mesenchymal Stem Cells., Cytotherapy
16,
915, 2014 |
Human
|
Human, adult |
Adipose derived stem |
Collagenase Type 1: 0.15%
|
DMEM |
Koellensperger, E., Bollinger, N., Dexheimer, V., Gramley, F., Germann, G. and Leimer, U.: Choosing the Right Type of Serum for Different Applications of Human Adipose Tissue-Derived Stem Cells: Influence on Proliferation and Differentiation Abilities., Cytotherapy
16,
789, 2014 |
Human
|
Human |
Umbilical mesenchymal stem |
Collagenase Type 2: 0.1%
Trypsin: 0.25%
|
DMEM |
Hang, H., Yu, Y., Wu, N., Huang, Q., Xia, Q. and Bian, J.: Induction of Highly Functional Hepatocytes from Human Umbilical Cord Mesenchymal Stem Cells by HNF4α Transduction., PLoS ONE
9,
e104133, 2014 |
Human
|
Human |
Cardiac stem |
Collagenase Type 2: 0.25%
|
Joklik modified Eagle’s |
Avolio, E., Gianfranceschi, G., Cesselli, D., Caragnano, A., Athanasakis, E., Katare, R., Meloni, M., Palma, A., Barchiesi, A., Vascotto, C., Toffoletto, B., Mazzega, E., Finato, N., Aresu, G. and Livi, U.: Ex vivo Molecular Rejuvenation Improves the Therapeutic Activity of Senescent Human Cardiac Stem Cells in a Mouse Model of Myocardial Infarction., Stem Cells 32,
2373, 2014 |
Human
|
Human |
Mesenchymal stromal |
Collagenase Type 1: 0.1%
|
PBS |
Najar, M, Rodrigues, R, Buyl, K, Branson, S, Vanhaecke, T, Lagneaux, L, Rogiers, V and De Kock, J: Proliferative and Phenotypical Characteristics of Human Adipose Tissue-Derived Stem Cells: Comparison of Ficoll Gradient Centrifugation and Red Blood Cell Lysis Buffer Treatment Purification Methods., Cytotherapy
16,
1220-8, 2014 |
Human
|
Human |
Skin fibroblasts |
Collagenase Type 1: 1000 u/ml
|
DMEM |
Douvaras, P., Wang, J., Zimmer, M., Hanchuk, S., O'Bara, M., Sadiq, S., Sim, F., Goldman, J. and Fossati, V.: Efficient Generation of Myelinating Oligodendrocytes from Primary Progressive Multiple Sclerosis Patients by Induced Pluripotent Stem Cells., Stem Cell Reports
3,
250, 2014 |
Human
|
Human |
Adipose stromal stem |
Collagenase animal free: 200 u/ml
|
DMEM/Hams F-12 |
Carvalho, P., Gimble, J., Dias, I., Gomes, M. and Reis, R.: Xenofree Enzymatic Products for the Isolation of Human Adipose-Derived Stromal/Stem Cells., Tiss Eng
19,
473-8, 2013 |
Human
|
Human, adult |
Epidermal stem cells |
Neutral Protease: 2 u/ml
Trypsin: 2.5%
|
DMEM |
Nagel, S., Rohr, F., Weber, C., Kier, J., Siemers, F., Kruse, C., Danner, S., Brandenburger, M. and Matthiessen, A.: Multipotent Nestin-Positive Stem Cells Reside in the Stroma of Human Eccrine and Apocrine Sweat Glands and can be Propagated Robustly In Vitro., PLoS ONE
8,
e78365, 2013 |
Human
|
Human |
Dental pulp stem |
Collagenase Type 1: 0.3%
Neutral Protease: 0.4%
|
DMEM/F12 |
Bonnamain, V., Thinard, R., Sergent-Tanguy, S., Huet, P., Bienvenu, G., Naveilhan, P., Farges, J. and Alliot-Licht, B.: Human Dental Pulp Stem Cells Cultured in Serum-Free Supplemented Medium., Front Physiol
4,
357, 2013 |
Human
|
Human |
Umbilical cord mesenchymal stem |
Collagenase: 0.1-0.2%
Trypsin: 0.1%
|
DMEM |
Han, Y., Tao, R., Sun, T., Chai, J., Xu, G. and Liu, J.: Optimization of Human Umbilical Cord Mesenchymal Stem Cell Isolation and Culture Methods., Cytotechnology
65,
819, 2013 |
Human
|
Human |
Endothelial colony forming |
Collagenase Type 1: 0.1%
Deoxyribonuclease I: 0.1%
Neutral Protease: 0.075%
|
HBSS |
Patel, J., Seppanen, E., Chong, M., Yeo, J., Teo, E., Chan, J., Fisk, N. and Khosrotehrani, K.: Prospective Surface Marker-Based Isolation and Expansion of Fetal Endothelial Colony-Forming Cells from Human Term Placenta., Stem Cells Transl Med 2,
839-47, 2013 |
Human
|
Human |
Stromal vascular fraction |
Collagenase Type 1: 0.075%
|
PBS |
Doi, K., Tanaka, S., Iida, H., Eto, H., Kato, H., Aoi, N., Kuno, S., Hirohi, T. and Yoshimura, K.: Stromal Vascular Fraction Isolated from Lipo-Aspirates Using an Automated Processing System: Bench and Bed Analysis., J Tissue Eng Regen Med
7,
864, 2013 |
Human
|
Human |
Alveolar epithelial |
Neutral Protease: 2 u/ml
Trypsin: 0.25%
Elastase: 10 u/ml
|
Bronchial Epithelial Growth Medium |
Barkauskas, C., Cronce, M., Rackley, C., Bowie, E., Keene, D., Stripp, B., Randell, S., Noble, P. and Hogan, B.: Type 2 Alveolar Cells are Stem Cells in Adult Lung, J Clin Invest 123,
3025, 2013 |
Human
|
Human |
Dental pulp stem cells |
Collagenase Type 1: 0.3%
Neutral Protease: 0.4%
|
DMEM |
Salmon, B., Bardet, C, Khaddam, M. Naji, J., Coyac, B., Baroukh, B., Letourneur, F., Lesieur, J., Decup, F., Le Denmat, D., Nicoletti, A., Poliard, A, Rowe, P., Huet, E., Vital, S. and Linglart, A.: MEPE-Derived ASARM Peptide Inhibits Odontogenic Differentiation of Dental Pulp Stem Cells and Impairs Mineralization in Tooth Models of X-linked Hypophosphatemia., PLoS ONE
8,
e56749, 2013 |
Human
|
Human |
Melanoma |
Collagenase Type 4: 0.1%
Deoxyribonuclease I: 0.01%
|
PBS |
Welte, Y., Davies, C., Schafer, R. and Regenbrecht, C.: Patient Derived Cell Culture and Isolation of CD133+ Putative Cancer Stem Cells from Melanoma., J Vis Exp
73,
e50200, 2013 |
Human
|
Human |
Cancer stem |
Trypsin: 0.25%
Collagenase: 0.05%
Hyaluronidase: 0.005%
|
RPMI |
Dobbin, Z. and Landen, C.: Isolation and Characterization of Potential Cancer Stem Cells from Solid Human Tumors--Potential Applications., Curr Protoc Pharmacol
63,
Unit 14.28., 2013 |
Human
|
Human |
Dental pulp derived stem |
Collagenase Type 1: 0.3%
Neutral Protease: 0.4%
|
DMEM |
Sakai, K., Yamamoto, A., Matsubara, K., Nakamura, S., Naruse, M., Yamagata, M., Sakamoto, K., Tauchi, R., Wakao, N., Imagama, S., Hibi, H., Kadomatsu, K., Ishiguro, N. and Ueda, M: Human Dental Pulp-Derived Stem Cells Promote Locomotor Recovery After Complete Transection of the Rat Spinal Cord by Multiple Neuro-Regenerative Mechanisms., J Clin Invest 122,
80, 2012 |
Human
|
Human |
Adipose-derived stem |
Collagenase Type 1: 0.1%
|
PBS |
Cervelli, V., Scioli, M., Gentile, P., Doldo, E., Bonanno, E., Spagnoli, L. and Orlandi, A.: Platelet-Rich Plasma Greatly Potentiates Insulin-Induced Adipogenic Differentiation of Human Adipose-Derived Stem Cells Through a Serine/Threonine Kinase Akt-dependent Mechanism and Promotes Clinical Fat Graft Maintenance., Stem Cells Transl Med 1,
206-20, 2012 |
Human
|
Human |
Mesenchymal stem |
Collagenase Type 1: 0.4%
Deoxyribonuclease I: 0.01%
|
DMEM/F12 |
Shalini, V., Pratheep, S., Muhammad, A., Sharmili, V., Elizabeth, G. and Rajesh, R.: Generation and Characterisation of Human Mesenchymal Stem Cells Derived From Umbilical Cord and Placenta, Regenerative Research 1,
48, 2012 |
Human
|
Human |
Wharton jelly mesenchymal stem |
Collagenase Type 2: 0.1%
Trypsin: 0.25%
|
DMEM |
Salehinejad, P., Alitheen, N., Ali, A., Omar, A., Mohit, M., Janzamin, E., Samani, F., Torshizi, Z. and Nematollahi-Mahani, S.: Comparison of Different Methods for the Isolation of Mesenchymal Stem Cells from Human Umbilical Cord Wharton's Jelly., In Vitro Cell Dev Biol Anim 48,
75, 2012 |
Human
|
Human |
Placental mesenchymal stem |
Collagenase Type 2: 0.4%
Deoxyribonuclease I: 0.01%
|
DMEM |
Vellasamy, S., Sandrasaigaran, P., Vidyadaran, S., George, E. and Ramasamy, R.: Isolation and Characterisation of Mesenchymal Stem cells Derived from Human Placenta Tissue., World J Stem Cells
4,
53-61, 2012 |
Human
|
Human |
Adipose derived stromal vascular |
Collagenase Type 1: 0.1%
|
PBS |
Gentile, P., Orlandi, A., Scioli, M., Di Pasquali, C., Bocchini, I. and Cervelli, V.: Concise Review: Adipose-Derived Stromal Vascular Fraction Cells and Platelet-Rich Plasma: Basic and Clinical Implications for Tissue Engineering Therapies in Regenerative Surgery., Stem Cells Transl Med 1,
230-6, 2012 |
Human
|
Human |
Perivascular stem |
Collagenase Type 2: 0.1%
|
DMEM |
James, A., Zara, J., Corselli, M., Askarinam, A., Zhou, A., Hourfar, A., Nguyen, A., Megerdichian, S., Asatrian, G., Pang, S., Stoker, D., Zhang, X., Wu, B., Ting, K., Peault, B. and Soo, C.: An Abundant Perivascular Source of Stem Cells for Bone Tissue Engineering., Stem Cells Transl Med 1,
673, 2012 |
Human
|
Human |
Chorionic mesenchymal |
Collagenase Type 1: 0.1%
Neutral Protease: 2.5 u/ml
Trypsin: 0.25%
|
MEM |
Nazarov, I., Lee, J., Soupene, E., Etemad, S., Knapik, D., Green, W., Bashkirova, E., Fang, X., Matthay, M., Kuypers, F. and Serikov, V.: Multipotent Stromal Stem Cells from Human Placenta Demonstrate High Therapeutic Potential., Stem Cells Transl Med 1,
359-72, 2012 |
Human
|
Human, fetal |
Liver hematopoietic |
Collagenase Type 4: 0.1%
Hyaluronidase: 0.1%
Deoxyribonuclease I: 2 u/ml
|
RPMI |
Vatakis, D., Bristol, G., Kim, S., Levin, B., Liu, W., Radu, C., Kitchen, S. and Zack, J.: Using the BLT Humanized Mouse as a Stem Cell Based Gene Therapy Tumor Model., J Vis Exp
70,
e4181, 2012 |
Human
|
Human, male 40-60 years |
Adipose derived stem cells |
Collagenase: 0.25%
Deoxyribonuclease I: 0.002%
|
PBS |
Blasi, A., Martino, C., Balducci, L., Saldarelli, M., Soleti, A., Navone, S., Canzi, L., Cristini, S., Invernici, G., Parati, E. and Alessandri, G.: Dermal Fibroblasts Display Similar Phenotypic and Differentiation Capacity to Fat-Derived Mesenchymal Stem Cells, but Differ in Anti-Inflammatory and Angiogenic Potential, Vasc Cell
3,
5, 2011 |
Human
|
Human |
Umbilical cord stromal stem |
Collagenase Type 4: 0.08%
Neutral Protease: 0.138%
Hyaluronidase: 0.02%
|
DMEM |
Farias, V., Linares-Fernandez, J., Penalver, J., Paya Colmenero, J., Ferron, G., Duran, E., Fernandez, R., Olivares, E., O'Valle, F., Puertas, A., Oliver, F. and Ruiz de Almodovar, J.: Human Umbilical Cord Stromal Stem Cell Express CD10 and Exert Contractile Properties., Placenta 32,
86, 2011 |
Human
|
Human |
Vascular wall-resident multipotent stem cells |
Collagenase Type 2: 0.2%
Elastase: 5 u/ml
|
see reference |
Klein, D., Weisshardt, P., Kleff, V., Jastrow, H., Jakob, H., and Ergun, S.: Vascular Wall-Resident CD44+ Multipotent Stem Cells Vive Rise to Pericytes and Smooth Muscle Cells and Contribute to New Vessel Maturation., PLoS ONE
6,
e20540, 2011 |
Human
|
Human |
Adipose derived stem |
Collagenase Type 1: 0.1%
|
DMEM/Ham's F-12 |
Yu, G. Floyd, ZE., Wu, X., Halvorsen, Y. and Gimble, J.: Isolation of Human Adipose-Derived Stem Cells from Lipoaspirates., Methods Mol Biol 702,
17-27, 2011 |
Human
|
Human |
Adipose derived mesenchymal |
Collagenase Type 1: 0.1%
|
L-DMEM |
Yang, X., He, X., He, J., Zhang, L., Su, X., Dong, Z., Xu, Y., Li, Y. and Li, YL.: High Efficient Isolation and Systematic Identification of Human Adipose-Derived Mesenchymal Stem Cells., J Biomed Sci
18,
59, 2011 |
Human
|
|
Brain tumor |
Collagenase Type 1: 0.04%
Hyaluronidase: 0.01%
Deoxyribonuclease I: 0.02%
Neutral Protease: 0.008%
|
DMEM/F12 |
Hussein, D., Punjaruk, W., Storer, L., Shaw, L., Othman, R., Ottoman, R., Peet, A., Miller, S., Bandopadhyay, G., Heath, R., Kumari, R., Bowman, K., Braker, P., Rahman, R., Jones, G., Watson, S. and Lowe, J.: Pediatric Brain Tumor Cancer Stem Cells: Cell Cycle Dynamics, DNA Repair, and Etoposide Extrusion., Neuro Oncol
13,
70-83, 2011 |
Human
|
Human |
Glioma stem cells |
PDS kit: per instructions
|
neurobasal medium |
Hjelmeland, A., Wu, Q., Wickman, S., Eyler, C., Heddleston, J., Shi, Q., Lathia, J., Macswords, J., Lee, J., McLendon, R. and Rich, J.: Targeting A20 Decreases Glioma Stem Cell Survival and Tumor Growth., PLoS Biol
8,
e1000319, 2010 |
Human
|
Human |
Dental pulp and apical papilla stem cells |
Collagenase Type 1: 0.3%
Neutral Protease: 0.4%
|
MEM |
Huang, G., Yamaza, T., Shea, L., Djouad, F., Kuhn, N., Tuan, R. and Shi, S.: Stem/Progenitor Cell-Mediated De Novo Regeneration of Dental Pulp with Newly Deposited Continuous Layer of Dentin in an In Vivo Model., Tissue Eng Part A
16,
605, 2010 |
Human
|
Human |
Corneal stromal stem |
Neutral Protease: 1.2 u/ml
Collagenase: 0.1%
|
DMEM |
Du, Y., Roh, D., Funderburgh, M., Mann, M., Marra, K., Rubin, J., Li, X. and Funderburgh, J.: Adipose-Derived Stem Cells Differentiate to Keratocytes In Vitro., Mol Vis
16,
2680, 2010 |
Human
|
Human |
Adipose tissue-derived stem |
Collagenase Type 2: 1.0%
|
DMEM/F12 |
Tan, H., DeFail, A., Rubin, J., Chu, C. and Marra, K.: Novel Multiarm PEG-Based Hydrogels for Tissue Engineering., J Biomed Mater Res 92,
979, 2010 |
Human
|
Human |
Umbilical cord mesenchymal |
Hyaluronidase: 0.05%
Collagenase: 0.08%
|
DMEM |
Zeddou, M., Briquet, A., Relic, B., Josse, C., Malaise, M., Gothot, A., Lechanteur, C. and Beguin, Y.: The Umbilical Cord Matrix is a Better Source of Mesenchymal Stem Cells (MSC) Than the Umbilical Cord Blood., Cell Biol Int 34,
693-701, 2010 |
Human
|
Human |
Adipose stem |
Collagenase Type 1: 0.1%
|
DMEM |
Hareendran, S., Sathishkumar, S., Abbas, S., Mackay, A. and Rajan, P.: A Novel Composition for the Culture of Human Adipose Stem Cells which Includes Complement C3., Cytotechnology
62,
389-402, 2010 |
Human
|
Human |
Adipose stromal |
Collagenase Type 1: 0.1%
|
DMEM/Hams F-12 |
Yu, G., Wu, X., Dietrich, M., Polk, P., Scott, LK, Ptitsyn, A. and Gimble, J.: Yield and Characterization of Subcutaneous Human Adipose-Derived Stem Cells by Flow Cytometric and Adipogenic mRNA Analyzes., Cytotherapy
12,
538, 2010 |
Human
|
Human |
Colonic epithelial |
Collagenase: 150 u/ml
Neutral Protease: 0.04 mg/ml
|
Basal X media |
Roig, A., Eskiocak, U., Hight, S., Kim, S., Delgado, O., Souza, R., Spechler, S., Wright, W. and Shay, J.: Immortalized Epithelial Cells Derived from Human Colon Biopsies Express Stem Cell Markers and Differentiate In Vitro., Gastroenterol 138,
1012, 2010 |
Human
|
Human, 40-65 year |
Adult human adipose stem cells |
Collagenase Type 2: 0.075%
|
DMEM |
Sun, N., Panetta, N., Gupta, D., Wilson, K., Lee, A., Jia, F., Hu, S., Cherry, A., Robbins, R., Longaker, M. and Wu, J.: Feeder-Free Derivation of Induced Pluripotent Stem Cells from Adult Human Adipose Stem Cells., Proc Natl Acad Sci U S A 106,
15720, 2009 |
Human
|
Human |
Filum terminale neural progenitor |
Trypsin: see reference
|
DMEM/F-12 |
Varghese, M., Olstorn, H., Berg-Johnsen, J., Moe, M., Murrell, W. and Langmoen, I.: Isolation of Human Multipotent Neural Progenitors from Adult Filum Terminale., Stem Cells Dev
18,
603, 2009 |
Human
|
Human |
Skin and keloid progenitor |
Collagenase Type 1: 0.4%
Neutral Protease: 0.3%
|
PBS |
Zhang, Q., Yamaza, T., Kelly, AP, Shi, S., Wang, S., Brown, J., Wang, L., French, S., Shi, S. and Le, A.: Tumor-like Stem Cells Derived from Human Keloid are Governed by the Inflammatory Niche Driven by IL-17/IL-6 Axis., PLoS ONE
4,
e7798, 2009 |
Human
|
Human |
Colon cancer |
Collagenase Type 1: 300 u/ml
Hyaluronidase: 100 u/ml
|
DMEM/F12 |
Varnat, F., Duquet, A., Malerba, M., Zbinden, M., Mas, C., Gervaz, P. and Ruiz i Altaba, A.: Human Colon Cancer Epithelial Cells Harbour Active HEDGEHOG-GLI Signalling that is Essential for Tumour Growth, Recurrence, Metastasis and Stem Cell Survival and Expansion., EMBO Mol Med
1,
338-51, 2009 |
Human
|
Human |
Adipose stromal |
Collagenase Type 1: 0.1%
|
DMEM |
Cai, L., Johnstone, B., Cook, T., Tan, J., Fishbein, M., Chen, P. and March, K.: IFATS Collection: Human Adipose Tissue-Derived Stem Cells Induce Angiogenesis and Nerve Sprouting Following Myocardial Infarction, in Conjunction with Potent Preservation of Cardiac Function., Stem Cells 27,
230, 2009 |
Human
|
Human |
Tumor |
Collagenase Type 4: 0.1%
Hyaluronidase: 0.07%
Deoxyribonuclease I: 0.04%
|
see reference |
Sauvageot, C., Weatherbee, J., Kesari, S., Winters, S., Barnes, J., Dellagatta, J., Ramakrishna, N., Stiles, C., Kung, A., Kieran, M. and Wen, P.: Efficacy of the HSP90 Inhibitor 17-AAG in Human Glioma Cell Lines and Tumorigenic Glioma Stem Cells.,Neuro Oncol Vol. 11,,
,
109, 2009 |
Human
|
Human |
Colonic epithelial |
Collagenase Type 4: 0.1%
|
not listed |
Huang, E., Hynes, M., Zhang, T., Ginestier, C., Dontu, G., Appelman, H., Fields, J., Wicha, M. and Boman, B.: Aldehyde Dehydrogenase 1 is a Marker for Normal and Malignant Human Colonic Stem Cells (SC) and Tracks SC Overpopulation During Colon Tumorigenesis., Cancer Res 69,
3382-9, 2009 |
Human
|
Human, male |
Spermatogonial stem cells |
Collagenase: 1%
Deoxyribonuclease I: 0.22%
Trypsin: 0.4%
|
DMEM |
Kossack, N., Meneses, J., Shefi, S., Nguyen, H., Chavez, S., Nicholas, C., Gromoll, J., Turek, P. and Reijo-Pera, R.: Isolation and Characterization of Pluripotent Human Spermatogonial Stem Cell-Derived Cells., Stem Cells ,
, 2008 |
Human
|
Human |
Muscle derived multiprogenitor cells |
Collagenase Type 2: 0.05%
|
DMEM |
Nesti, L., Jackson, W., Shanti, R., Koehler, S., Aragon, A., Bailey, J., Sracic, M., Freedman, B., Giuliani, J. and Tuan, R.: Differentiation Potential of Multipotent Progenitor Cells Derived from War-Traumatized Muscle Tissue., J Bone Joint Surg Am
90,
2390, 2008 |
Human
|
Human |
Adipose derived stem cells |
Collagenase Type 1: 0.075%
|
DMEM |
Jeong, J.: Adipose Stem Cells as a Clinically Available and Effective Source of Adult Stem Cell Therapy, Int J Stem Cells 1,
43, 2008 |
Human
|
Human |
Hepatic stem cells and heptoblasts |
Collagenase Type 4: 0.014-0.06%
|
various |
Wauthier, E., Schmelzer, E., Turner, W., Zhang, L., LeCluyse, E., Ruiz, J., Turner, R., Furth, M., Kubota, H., Lozoya, O., Barbier, C., McClelland, R., Yao, H., Moss, N., Bruce, A., Ludlow, J. and Reid, L.: Hepatic Stem Cells and Hepatoblasts: Identification, Isolation, and Ex Vivo Maintenance., Methods Cell Biol 86,
137, 2008 |
Human
|
Human |
Umbilical mesenchymal stem |
Collagenase: 0.1%
|
DMEM |
Secco, M., Zucconi, E., Vieira, N., Fogaca, L.,Cerqueira, A., Carvalho, M., Jazedje, T., Okamoto, O., Muotri, A. and Zatz, M.: Multipotent Stem Cells from Umbilical Cord: Cord is Richer Than Blood!, Stem Cells 26,
146, 2008 |
Human
|
Human |
Stem |
Collagenase: 280 u/ml
|
D-PBS |
Pilgaard, L., Lund, P., Rasmussen, J., Fink, T. and Zachar, V.: Comparative Analysis of Highly Defined Proteases for the Isolation of Adipose Tissue-Derived Stem Cells., Regen Med
3,
705-15, 2008 |
Human
|
Human |
Bone marrow derived MSC |
Trypsin: 0.05%
Papain: 0.0025%
|
DMEM |
Welter, J., Solchaga, L.and Penick, K.: Simplification of Aggregate Culture of Human Mesenchymal Stem Cells as a Chondrogenic Screening Assay, Biotechniques
42,
732, 2007 |
Human
|
Human, 8-12 year |
Tendon stem/progenitor |
Collagenase Type 1: 0.3%
Neutral Protease: 0.4%
|
DMEM |
Bi Yanming, Ehirchiou Driss, Kilts Tina M, Inkson Colette A, Embree Mildred C, Sonoyama Wataru, Li Li, Leet Arabella I, Seo Byoung-Moo, Zhang Li, Shi Songtao, Young Marian F: Identification of tendon stem/progenitor cells and the role of the extracellular matrix in their niche, Nat Med 13,
1219-27, 2007 |
Human
|
Human, male |
Adipose derived adult stem cells |
Collagenase Type 1: 0.1%
|
DMEM |
Lei, L., Liao, W., Sheng, P., Fu, M., He, A. and Huang, G.: Biological Character of Human Adipose-Derived Adult Stem Cells and Influence of Donor Age on Cell Replication in Culture., Sci China C Life Sci
50,
320, 2007 |
Human
|
Human |
Pancreatic cancer stem cells |
Collagenase Type 4: 200 u/ml
|
medium 199 |
Li, C., Heidt, D., Dalerba, P., Burant, C., Zhang, L., Adsay, V., Wicha, M., Clarke, M. and Simeone, D.: Identification of Pancreatic Cancer Stem Cells., Cancer Res 67,
1030, 2007 |
Human
|
Human |
Adipose derived stem cells |
Collagenase Type 1: 0.1%
|
DMEM/F12 |
Kilroy, G., Foster, S., Wu, X., Ruiz, J., Sherwood, S., Heifetz, A., Ludlow, J., Stricker, D., Potiny, S., Green, P., Halvorsen, Y., Cheatham, B., Storms, R. and Gimble, J.: Cytokine Profile of Human Adipose-Derived Stem Cells: Expression of Angiogenic, Hematopoietic, and Pro-Inflammatory Factors., J Cell Physiol 212,
702-9, 2007 |
Human
|
Human |
Oral epithelial |
Neutral Protease: 1.2 u/ml
Trypsin: 0.05%
|
PBS |
Nakamura, T., Endo, K. and Kinoshita, S.: Identification of Human Oral Keratinocyte Stem/Progenitor Cells by Neurotrophin Receptor p75 and the Role of Neurotrophin/p75 Signaling., Stem Cells 25,
628-38, 2007 |
Human
|
Human |
Mesenchymal stem |
Collagenase Type 2: 10%
Neutral Protease: see reference
|
CMF-DPBS |
Steigman, S. and Fauza, D.: Isolation of Mesenchymal Stem Cells from Amniotic Fluid and Placenta., Curr Protoc Stem Cell Biol
Chapter 1,
Unit 1E.2, 2007 |
Human
|
Human, adult |
Adipose-derived adult stem |
Collagenase Type 1: 0.1%
|
DMEM/F-12 Ham's |
Mitchell James B, McIntosh Kevin, Zvonic Sanjin, Garrett Sara, Floyd Z Elizabeth, Kloster Amy, Di Halvorsen Yuan, Storms Robert W, Goh Brian, Kilroy Gail, Wu Xiying, Gimble Jeffrey M: Immunophenotype of human adipose-derived cells: temporal changes in stromal-associated and stem cell-associated markers, Stem Cells 24,
376-85, 2006 |
Human
|
Human |
Mesenchymal stem |
Collagenase Type 1: 0.075%
|
DMEM |
Kern Susanne, Eichler Hermann, Stoeve Johannes, Kluter Harald, Bieback Karen: Comparative analysis of mesenchymal stem cells from bone marrow, umbilical cord blood, or adipose tissue, Stem Cells 24,
1294-301, 2006 |
Human
|
Human |
Placental mesenchymal stem |
Collagenase Type 2: 270 u/ml
Neutral Protease: 2.4 u/ml
|
MEM |
Portmann-Lanz CBettina, Schoeberlein Andreina, Huber Alexander, Sager Ruth, Malek Antoine, Holzgreve Wolfgang, Surbek DanielV: Placental mesenchymal stem cells as potential autologous graft for pre- and perinatal neuroregeneration, Am J Obstet Gynecol
194,
664-73, 2006 |
Human
|
Human |
Stromal stem cells |
Collagenase Type 1: 0.2%
|
HBSS |
Boquest Andrew C, Shahdadfar Aboulghassem, Brinchmann Jan E, Collas Philippe: Isolation of stromal stem cells from human adipose tissue, Methods Mol Biol 325,
35-46, 2006 |
Human
|
Human, female |
Adipose derived adult stem cells |
Collagenase Type 1: 0.1%
|
DMEM/F-12 |
Guilak, F., Lott, K., Awad, H., Cao, Q., Hicok, K., Fermor, B. and Gimble, J.: Clonal Analysis of the Differentiation Potential of Human Adipose-Derived Adult Stem Cells., J Cell Physiol 206,
229, 2006 |
Human
|
Human |
Mesenchymal stem |
Collagenase Type 1: 0.1%
|
HBSS |
Jeon Eun Su, Song Hae Young, Kim Mi Ra, Moon Hyun Jung, Bae Yong Chan, Jung Jin Sup, Kim Jae Ho: Sphingosylphosphorylcholine induces proliferation of human adipose tissue-derived mesenchymal stem cells via activation of JNK, J Lipid Res 47,
653-64, 2006 |
Human
|
Human |
Hair follicular epithelial |
Neutral Protease: 1.25%
Trypsin: 0.25%
|
DMEM/F-12 |
Yu Hong, Fang Dong, Kumar Suresh M, Li Ling, Nguyen Thiennga K, Acs Geza, Herlyn Meenhard, Xu Xiaowei: Isolation of a novel population of multipotent adult stem cells from human hair follicles, Am J Pathol 168,
1879-88, 2006 |
Human
|
Human |
Adult stem cells |
Collagenase Type 1: 0.1%
|
PBS |
Devireddy, R., Thirumala, S. and Gimble, J.: Cellular Response of Adipose Derived Passage-4 Adult Stem Cells to Freezing Stress., J Biomech Eng 127,
1081, 2005 |
Human
|
Human |
Adipocytes, stromal vascular |
Collagenase: 0.2%
|
HBSS |
Boquest Andrew C, Shahdadfar Aboulghassem, Fronsdal Katrine, Sigurjonsson Olafur, Tunheim Siv H, Collas Philippe, Brinchmann Jan E: Isolation and transcription profiling of purified uncultured human stromal stem cells: alteration of gene expression after in vitro cell culture, Mol Biol Cell
16,
1131-41, 2005 |
Human
|
Human, female |
Adipose derived adult stem cells |
Collagenase Type 1: 0.1%
|
DMEM-Ham's F-12 |
Aust, L., Devlin, B., Foster, S., Halvorsen, Y., Hicok, K., du Laney, T., Sen, A., Willingmyre, G. and Gimble, J.: Yield of Human Adipose-Derived Adult Stem Cells from Liposuction Aspirates., Cytometry 6,
7-14, 2004 |
Human
|
Human |
Stromal vascular, adipocytes, stem |
Collagenase: 300 u/ml
|
PBS |
Miranville A, Heeschen C, Sengenes C, Curat CA, Busse R, Bouloumie A: Improvement of postnatal neovascularization by human adipose tissue-derived stem cells, Circulation 110,
349-55, 2004 |
Human
|
Human, neonatal |
Keratinocytes |
Neutral Protease: 0.4%
Collagenase: 0.3%
|
DMEM |
Li, A., Pouliot, N., Redvers, R., and Kaur, P.: Extensive tissue-regenerative capacity of neonatal human keratinocytes stem cells and their progeny, J Clin Invest 113,
390-400, 2004 |
Human
|
Human, adult, male |
Human epidermal keratinocyte stem cells |
Neutral Protease: 0.5%
|
DMEM |
Papini S, Cecchetti D, Campani D, Fitzgerald W, Grivel J, Chen S, Margolis L, Revoltella R.: Isolation and Clonal Analysis of Human Epidermal Keratinocyte Stem Cells in Long-term Culture, Stem Cells 21,
481, 2003 |
Human
|
Human |
Umbilical vein mesenchymal stem cells |
Collagenase: 1%
|
PBS |
Covas DT, Siufi JL C, Silva AR L, Orellana MD: Isolation and culture of umbilical vein mesenchymal stem cells, Braz J Med Biol Res
36,
1179-83, 2003 |
Human
|
Human, 7-8 yr |
Stem cells Human exfoliated deciduous teeth |
Collagenase Type 1: 0.3%
Neutral Protease: 0.4%
|
see reference |
Miura, M., Gronthos, S, Zhao, M, Lu, B, Fisher, L, Robey, P, and Shi, S.: SHED: Stem Cells from Human Exfoliated Deciduous Teeth, Proc Natl Acad Sci U S A 100,
5807, 2003 |
Human
|
Human, fetal |
Epithelial progenitor |
Collagenase: 0.03%
|
DMEM |
Malhi, H., Irani, A., Gagandeep, S. and Gupta, S.: Isolation of Human Progenitor Liver Epithelial Cells with Extensive Replication Capacity and Differentiation into Mature Hepatocytes., J Cell Sci
115,
2679, 2002 |
Human
|
Human |
Processed lipoaspirate cells |
Collagenase Type 1: 0.075%
|
PBS |
Zuk, P., Zhu, M., Ashjian, P., De Ugarte, D., Huang, J., Mizuno, H., Alfonso, Z., Fraser, J., Benhaim, P., and Hedrick, M.: Human adipose tissue is a source of multipotent stem cells, Mol Biol Cell
13,
4279-95, 2002 |
Human
|
Human, adult |
Human skin mast cells |
Collagenase Type 2: 0.15%
Hyaluronidase: 0.07%
Deoxyribonuclease I: 0.03%
|
HBSS |
Kambe, N, Kambe, M, Kochan, J, and Schwartz ,L.: Human Skin-derived Mast Cells Can Proliferate While Retaining Their Characteristic Functional and Protease Phenotypes, Blood 97,
2045, 2001 |
Human
|
Human |
Stem, embryonic |
Neutral Protease: 0.01% - 0.02%
|
DMEM |
Zhang, S., Wernig, M., Duncan, I., Brustle, O., and Thomson, J.: In Vitro Differentiation of Transplantable Neural Precursors from Human Embryonic Stem Cells, Nat Biotechnol
19,
1129, 2001 |
Human
|
Human |
Central nervous system stem |
Collagenase: 0.1%
Hyaluronidase: 0.1%
|
HBSS |
Uchida, N., Buck, D., He, D., Reitsma, M., Masek, M., Phan, T., Tsukamoto, A., Gage, F. and Weissman, I.: Direct Isolation of Human Central Nervous System Stem Cells., Proc Natl Acad Sci U S A 97,
14720, 2000 |
Human
|
Human |
Embryonic stem |
Neutral Protease: 1%
Collagenase Type 4: 0.1%
|
DMEM |
Thomson JA, Itskovitz-Eldor J, Shapiro SS, Waknitz MA, Swiergiel JJ, Marshall VS, Jones JM: Embryonic stem cell lines derived from human blastocysts, Science 282,
1145-7, 1998 |
Human
|
Human |
Muscle-derived stem cells |
Trypsin: 0.25%
|
DMEM/F12 |
Alessandri Giulio, Pagano Stefano, Bez Alessandra, Benetti Anna, Pozzi Stefano, Iannolo Gioacchin, Baronio Manuela, Invernici Gloria, Caruso Arnaldo, Muneretto Claudio, Bisleri Gianluigi, Parati Eugenio: Isolation and culture of human muscle-derived stem cells able to differentiate into myogenic and neurogenic cell lineages, Lancet 364,
1872-83, |
Insect
|
Drosophila, larval |
Neuroblasts |
Collagenase Type 1: 0.1%
Papain: 0.1%
|
Rinaldini solution |
Berger, C., Harzer, H., Burkard, T., Steinmann, J., van der Horst, S., Laurenson, A., Novatchkova, M., Reichert, H. and Knoblich, J.: FACS Purification and Transcriptome Analysis of Drosophila Neural Stem Cells Reveals a Role for Klumpfuss in Self-Renewal., Cell Rep
2,
407, 2012 |
Monkey
|
Monkey |
Embryonic stem |
Collagenase Type 4: 0.08%
|
DMEM |
Chen, S., Revoltella, R., Papini, S., Michelini, M., Fitzgerald, W., Zimmerberg, J.,and Margolis, L.: Multilineage Differentiation of Rhesus Monkey Embryonic Stem cells in Three-dimensional Culture Systems, Stem Cells 21(3),
281, 2003 |
Monkey
|
Baboons, 1yr, 7yr, 14 yr |
Primate spermatogonial |
Collagenase Type 2: 0.1%
Trypsin: 0.05%
Deoxyribonuclease I: 0.1%
|
DMEM |
Nagano, M., McCarrey, J., and Brinster, R.: Primate Spermatogonial Stem Cells Colonize Mouse Testes, Biol Reprod 64,
1409, 2001 |
Mouse
|
Mouse, xenograft |
Pancreatic cancer |
Stemxyme 1: 0.2%
|
HBSS |
Pham, K., Delitto, D., Knowlton, A., Hartlage, E., Madhavan, R., Gonzalo, D., Thomas, R., Behrns, K., George, T., Hughes, S., Wallet, S., Liu, C., Trevino, J.: Isolation of Pancreatic Cancer Cells from a Patient-Derived Xenograft Model Allows for Practical Expansion and Preserved Heterogeneity in Culture., Am J Pathol 186,
, 2016 |
Mouse
|
Mouse, GFP transgenic |
Muscle derived NLSC |
Collagenase Type 2: 0.2%
|
DMEM |
Birbrair, A, Sattiraju, A, Zhu, D, Zulato, G, Batista, I, Nguyen, V, Messi M, Solingapuram Sai, K, Marini, F, Delbono, O and Mintz, A.: Novel Peripherally Derived Neural-Like Stem Cells as Therapeutic Carriers for Treating Glioblastomas., Stem Cells Transl Med ,
, 2016 |
Mouse
|
Mouse |
Adipose mesenchyal stromal |
Collagenase Type 2: 0.2%
|
DMEM |
Maria, O., Shalaby, M., Syme, A., Eliopoulos, N. and Muanza, T.: Adipose Mesenchymal Stromal Cells Minimize and Repair Radiation-Induced Oral Mucositis., Cytotherapy
18,
1129-45, 2016 |
Mouse
|
Mouse, 3-4 week |
Cardiac progenitor |
Collagenase Type 1: 0.1%
|
DMEM |
Wang, H., Chen, H., Feng, B., Wang, X., He, X., Hu, R., Yin, M., Wang, W., Fu, W. and Xu, Z.: Isolation and Characterization of a Sca-1+/CD31- Progenitor Cell Lineage Derived From Mouse Heart Tissue., BMC Biotechnol
14,
75, 2014 |
Mouse
|
Mouse |
Epithelial stem |
Collagenase: 2%
|
DMEM/F12 |
Chavez, M., Hu, J., Seidel, K., Li, C., Jheon, A., Naveau, A., Horst, O. and Klein, O.: Isolation and Culture of Dental Epithelial Stem Cells from the Adult Mouse Incisor., J Vis Exp
,
, 2014 |
Mouse
|
Mouse, P4 |
Enteric neural crest progenitors |
Neutral Protease: 0.5%
Collagenase animal free: 0.05%
|
DMEM/F12 |
Hotta, R., Stamp, L., Foong, J., McConnell, S., Bergner, A., Anderson, R., Enomoto, H., Newgreen, D., Obermayr, F., Furness, J. and Young, H.: Transplanted Progenitors Generate Functional Enteric Neurons in the Postnatal Colon., J Clin Invest 123,
1182, 2013 |
Mouse
|
Mouse |
Epithelial stem |
Trypsin: 0.25%
Collagenase Type 1: 0.4%
|
HBSS |
Jensen, U., Ghazizadeh, S. and Owens, D.: Isolation and Characterization of Cutaneous Epithelial Stem Cells., Methods Mol Biol 989,
61-9, 2013 |
Mouse
|
Mouse |
Colon tumor organoids |
Collagenase Type 4: 200 u/ml
Neutral Protease: 0.01%
|
DMEM |
Xue, X. and Shah, Y.: In Vitro Organoid Culture of Primary Mouse Colon Tumors., J Vis Exp
,
e50210, 2013 |
Mouse
|
Mouse, 8-12 week |
Pancreas organoid |
Collagenase: 0.012%
Neutral Protease: 0.012%
|
DMEM |
Huch, M., Bonfanti, P., Boj, S., Sato, T., Loomans, C., Van de Wetering, M., Sojoodi, M., Li, V., Schuijers, J., Gracanin, A., Ringnalda, F., Begthel, H., Hamer, K., Mulder, J., Van Es, J. and De Koning, E.: Unlimited In Vitro Expansion of Adult Bi-Potent Pancreas Progenitors Through the Lgr5/R-Spondin Axis., EMBO J
32,
2708-21, 2013 |
Mouse
|
Mouse |
Mammary tumor |
Collagenase: 0.15%
Hyaluronidase: 0.020%
|
DMEM/F12 |
Liu, X., Johnson, S., Liu, S., Kanojia, D., Yue, W., Singh, U., Wang, Q, Wang Qi, Nie, Q. and Chen H.: Nonlinear Growth Kinetics of Breast Cancer Stem Cells: Implications for Cancer Stem Cell Targeted Therapy., Sci Rep
3,
2473, 2013 |
Mouse
|
Mouse, 4-6 week |
Schwann |
Collagenase Type 1: 0.01%
Trypsin: 0.125%
|
DMEM |
Masaki, T., Qu, J., Cholewa-Waclaw, J., Burr, K., Raaum, R. and Rambukkana, A.: Reprogramming Adult Schwann Cells to Stem Cell-Like Cells by Leprosy Bacilli Promotes Dissemination of Infection., Cell
152,
51, 2013 |
Mouse
|
Mouse, adult |
Spermatogonial stem |
Trypsin: 0.05%
Collagenase Type 1: 0.03%
Deoxyribonuclease I: 80 u/ml
|
DMEM |
Martin, L. and Seandel, M.: Serial Enrichment of Spermatogonial Stem and Progenitor Cells (SSCs) in Culture for Derivation of Long-Term Adult Mouse SSC Lines., J Vis Exp
72,
e50017, 2013 |
Mouse
|
Mouse |
Prostate tumor |
Collagenase Type 4: 1,600 u/ml
|
DMEM/F12 |
Mazzoleni, S., Jachetti, E., Morosini, S., Grioni, M., Piras, I., Pala, M., Bulfone, A., Freschi, M., Bellone, M. and Galli, R.: Gene Signatures Distinguish Stage-Specific Prostate Cancer Stem Cells Isolated From Transgenic Adenocarcinoma of the Mouse Prostate Lesions and Predict the Malignancy of Human Tumors., Stem Cells Transl Med 2,
678, 2013 |
Mouse
|
Mouse, 10 week |
Synovial mesenchymal |
Collagenase: 0.1%
Deoxyribonuclease I: 0.005%
|
DMEM |
Futami, I., Ishijima, M., Kaneko, H., Tsuji, K., Ichikawa-Tomikawa, N., Sadatsuki, R., Muneta, T., Arikawa-Hirasawa, E., Sekiya, I. and Kaneko, K.: Isolation and Characterization of Multipotential Mesenchymal Cells from the Mouse Synovium., PLoS ONE
7,
e45517, 2012 |
Mouse
|
Mouse, 6-8 week |
Neural stem cells |
Papain: 0.01%
Neutral Protease: 0.1%
Deoxyribonuclease I: 0.01%
|
DMEM/F12 |
Bracko, O., Singer, T., Aigner, S., Knobloch, M., Winner, B., Ray, J., Clemenson, G., Suh, H., Couillard-Despres, S., Aigner, L., Gage, F. and Jessberger, S.: Gene Expression Profiling of Neural Stem Cells and their Neuronal Progeny Reveals IGF2 as a Regulator of Adult Hippocampal Neurogenesis., J Neurosci 32,
3376-87, 2012 |
Mouse
|
Mouse |
Intestinal organoids |
Collagenase Type 1: 800 u/ml
Neutral Protease: 0.013%
|
DMEM |
Barthel, E., Speer, A., Levin, D., Sala, F., Hou, X., Torashima, Y., Wigfall, C. and Grikscheit, T.: Tissue Engineering of the Intestine in a Murine Model., J Vis Exp
70,
e4279, 2012 |
Mouse
|
Mouse |
Adipose derived stem |
Collagenase Type 2: 0.1%
|
DMEM |
Takahashi, H., Haraguchi, N., Nishikawa, S., Miyazaki, S., Suzuki, Y., Mizushima, T., Nishimura, J., Takemasa, I., Yamamoto, H., Mimori, K., Ishii, H., Doki, Y. and Mori, M.: Biological and Clinical Availability of Adipose-Derived Stem Cells for Pelvic Dead Space Repair., Stem Cells Transl Med 1,
803, 2012 |
Mouse
|
Mouse |
Lung tumor |
Neutral Protease: 50 u/ml
Collagenase: 400 u/ml
Deoxyribonuclease I: 50 u/ml
|
DMEM |
Vaughan, A., Halbert, C., Wootton, S. and Miller, A.: Lung Cancer in Mice Induced by the Jaagsiekte Sheep Retrovirus Envelope Protein is not Maintained by Rare Cancer Stem Cells, but Tumorigenicity does Correlate with Wnt Pathway Activation., Mol Cancer Res
10,
86, 2012 |
Mouse
|
Mouse, P4-5 |
Neuroshere |
Trypsin: 0.25%
Papain: 100 u
Deoxyribonuclease I: 0.025%
|
Pro-N |
Ziegler, A., Schneider, J., Qin, M., Tyler, W., Pintar, J., Fraidenraich, D., Wood, T and Levison, S.: IGF-II Promotes Stemness of Neural Restricted Precursors., Stem Cells 30,
1265, 2012 |
Mouse
|
Mouse |
Nerve progenitors |
Collagenase Type 4: 0.025%
Trypsin NF 1:250: .025%
|
HBSS |
Salisbury, E., Lazard, Z., Ubogu, E., Davis, A. and Olmsted-Davis, E.: Transient Brown Adipocyte-Like Cells Derive from Peripheral Nerve Progenitors in Response to Bone Morphogenetic Protein 2., Stem Cells Transl Med 1,
874-85, 2012 |
Mouse
|
Mouse, 8-10 week |
Adipose derived stem |
Collagenase Type 1: 0.025%
|
HBSS |
Sugii, S., Kida, Y., Berggren, W. and Evans, R.: Feeder-Dependent and Feeder-Independent iPS Cell Derivation from Human and Mouse Adipose Stem Cells., Nat Protoc
6,
346, 2011 |
Mouse
|
Mouse |
Spleen, bone marrow endothelial |
Collagenase Type 4: 0.3-1.0%
Deoxyribonuclease I: 20 u/ml
|
PBS |
Shi, C., Jia, T., Mendez-Ferrer, S., Hohl, T., Serbina, N., Lipuma, L., Leiner, I., Li, M., Frenette, P. and Pamer, E.: Bone Marrow Mesenchymal Stem and Progenitor Cells Induce Monocyte Emigration in Response to Circulating Toll-Like Receptor Ligands., Immunity
34,
590, 2011 |
Mouse
|
Mouse, 8-10 week |
Lung mesenchymal stem |
Collagenase Type 2: 0.2%
|
HBSS |
Chow, K., Jun, D., Helm, K., Wagner, D. and Majka, S.: Isolation & Characterization of Hoechst(low) CD45(negative) Mouse Lung Mesenchymal Stem Cells., J Vis Exp
56,
e3159, 2011 |
Mouse
|
Mouse, neonatal |
Dental pulp stem |
Collagenase Type 4: 0.2%
Neutral Protease: 1.2 u/ml
|
PBS |
Janebodin, K., Horst, O., Ieronimakis, N., Balasundaram, G., Reesukumal, K., Pratumvinit, B. and Reyes, M.: Isolation and Characterization of Neural Crest-Derived Stem Cells from Dental Pulp of Neonatal Mice., PLoS ONE
6,
e27526, 2011 |
Mouse
|
Mouse |
CD133+ stem |
Collagenase Type 4: 0.05%
Pronase: 0.05%
Deoxyribonuclease I: 0.01%
|
DMEM/F12 |
Rountree, C., Ding, W., Dang, H., Vankirk, C. and Crooks, G.: Isolation of CD133+ Liver Stem Cells for Clonal Expansion., J Vis Exp
56,
e3183, 2011 |
Mouse
|
Mouse |
Stem and progenitor |
Collagenase Type 2: 0.2%
|
HBSS |
Han, J., Koh, Y., Moon, H., Ryoo, H., Cho, C., Kim, I. and Koh, G.: Adipose Tissue is an Extramedullary Reservoir for Functional Hematopoietic Stem and Progenitor Cells., Blood 115,
957, 2010 |
Mouse
|
Mouse, 6-8 week |
Bone marrow mesenchymal stem |
Collagenase Type 1: 0.25%
|
RPMI 1640 |
Xu, S., De Becker, A., Van Camp, B., Vanderkerken, K. and Van Riet, I.: An Improved Harvest and In Vitro Expansion Protocol for Murine Bone Marrow-Derived Mesenchymal Stem Cells., J Biomed Biotechnol
2010,
105940, 2010 |
Mouse
|
Mouse |
Endosteal cells |
Collagenase Type 1: 0.3%
|
DMEM |
Nakamura, Y., Arai, F., Iwasaki, H., Hosokawa, K., Kobayashi, I., Gomei, Y., Matsumoto, Y., Yoshihara, H. and Suda, T.: Isolation and Characterization of Endosteal Niche Cell Populations that Regulate Hematopoietic Stem Cells., Blood 116,
1422, 2010 |
Mouse
|
Mouse, 10 week |
Islets |
Collagenase: 0.2%
|
RPMI 1640 |
Kobayashi, T., Yamaguchi, T., Hamanaka, S., Kato-Itoh, M., Yamazaki, Y., Ibata, M., Sato, H., Lee, Y., Usui, J., Knisely, A., Hirabayashi, M. and Nakauchi, H.: Generation of Rat Pancreas in Mouse by Interspecific Blastocyst Injection of Pluripotent Stem Cells., Cell
142,
787, 2010 |
Mouse
|
Mouse |
Pancreatic cancer stem |
Collagenase Type 4: 200 u/ml
Neutral Protease: 0.6 u/ml
|
DMEM |
Rasheed, Z., Wang, Q. and Matsui, W.: Isolation of Stem Cells from Human Pancreatic Cancer Xenografts., J Vis Exp
43,
2169, 2010 |
Mouse
|
Mouse, adult |
Adult neural stem |
Trypsin: 0.05%
|
DMEM |
Deleyrolle, L. and Reynolds, B.: Isolation, Expansion, and Differentiation of Adult Mammalian Neural Stem and Progenitor Cells using the Neurosphere Assay., Methods Mol Biol 549,
91, 2009 |
Mouse
|
Mouse, 6-8 week |
Prostate epithelial/stem |
Collagenase Type 2: 0.5%
Trypsin: 0.05%
|
HBSS |
Burger, P., Gupta, R., Xiong, X., Ontiveros, C., Salm, S., Moscatelli, D. and Wilson, E.: High Aldehyde Dehydrogenase Activity: A Novel Functional Marker of Murine Prostate Stem/Progenitor Cells., Stem Cells 27,
2220-8, 2009 |
Mouse
|
Mouse |
Adipose tissue-derived stem |
Collagenase Type 1: 0.075%
|
Modified Eagles |
Cho, K., Park, H., Park, H., Jung, J., Jeon, S., Kim, Y. and Roh, H.: IFATS Collection: Immunomodulatory Effects of Adipose Tissue-Derived Stem Cells in an Allergic Rhinitis Mouse Model., Stem Cells 27,
259-65, 2009 |
Mouse
|
Mouse |
Bone marrow |
Collagenase: 0.2%
|
DMEM |
Morikawa, S., Mabuchi, Y., Kubota, Y., Nagai, Y., Niibe, K., Hiratsu, E., Suzuki, S., Miyauchi-Hara, C., Nagoshi, N., Sunabori, T., Shimmura, S., Miyawaki, A., Nakagawa, T., Suda, T., Okano, H. and Matsuzaki, Y.: Prospective Identification, Isolation, and Systemic Transplantation of Multipotent Mesenchymal Stem Cells in Murine Bone Marrow., J Exp Med 206,
2483-96, 2009 |
Mouse
|
Mouse |
Germ cells |
Collagenase Type 1: 100 u/ml
|
HBSS |
Breault, D., Min, I., Carlone, D., Farilla, L., Ambruzs, D., Henderson, D., Algra, S., Montgomery, R., Wagers, A. and Hole, N.: Generation of mTert-GFP Mice as a Model to Identify and Study Tissue Progenitor Cells., Proc Natl Acad Sci U S A 105,
10420, 2008 |
Mouse
|
Mouse |
Neural progenitor cell |
Papain: 10 ul/ml
|
PBS |
Hutton, S. and Pevny, L.: Isolation, Culture, and Differentiation of Progenitor Cells from the Central Nervous System., Cold Spring Harb. Protoc. 11,
5077, 2008 |
Mouse
|
Mouse, 3-6 week |
Ear mesenchymal stem |
Collagenase Type 1: 0.2%
|
DMEM/F12 |
Staszkiewicz, J., Gimble, J., Manuel, J. and Gawronska-Kozak, B.: IFATS Collection: Stem Cell Antigen-1-Positive Ear Mesenchymal Stem Cells Display Enhanced Adipogenic Potential., Stem Cells 26,
2666, 2008 |
Mouse
|
Mouse, postnatal |
Astrocytes |
Trypsin: 0.25%
Deoxyribonuclease I: 1,000 u/ml
|
HBSS |
Sher, F., Rossler, R., Brouwer, N., Balasubramaniyan, V., Boddeke, E. and Copray, S.: Differentiation of Neural Stem Cells Into Oligodendrocytes: Involvement of the Polycomb Group Protein Ezh2., Stem Cells 26,
2875, 2008 |
Mouse
|
Mouse, 12 week |
Mammary epithelial |
Collagenase: 0.3%
Hyaluronidase: 100 u/ml
Trypsin: 0.25%
Neutral Protease: 0.5%
Deoxyribonuclease I: 0.01%
|
see reference |
Taddei, I., Deugnier, M., Faraldo, M., Petit, V., Bouvard, D., Medina, D., Fassler, R., Thiery, J. and Glukhova, M.: Beta1 Integrin Deletion from the Basal Compartment of the Mammary Epithelium Affects Stem Cells, Nat Cell Biol 10,
716-22, 2008 |
Mouse
|
Mouse, embryonic |
Hematopoietic stem cells |
Collagenase: 0.1%
|
PBS |
Gekas, C., Rhodes, K. and Mikkola, H.: Isolation and Analysis of Hematopoietic Stem Cells from the Placenta., J Vis Exp
16,
e742, 2008 |
Mouse
|
Mouse, embryonic |
HES-BC cells |
Trypsin: 0.05%
|
DMEM |
Lu Shi-Jiang, Feng Qiang, Caballero Sergio, Chen Yu, Moore Malcolm A, Grant Maria B, Lanza Robert: Generation of functional hemangioblasts from human embryonic stem cells, Nat Methods
4,
501-9, 2007 |
Mouse
|
Mouse, 6-8 week |
Tendon stem/progenitor |
Collagenase Type 1: 0.3%
Neutral Protease: 0.4%
|
DMEM |
Bi Yanming, Ehirchiou Driss, Kilts Tina M, Inkson Colette A, Embree Mildred C, Sonoyama Wataru, Li Li, Leet Arabella I, Seo Byoung-Moo, Zhang Li, Shi Songtao, Young Marian F: Identification of tendon stem/progenitor cells and the role of the extracellular matrix in their niche, Nat Med 13,
1219-27, 2007 |
Mouse
|
Mouse |
Tumor |
Collagenase Type 3: 200 u/ml
|
RPMI-1640 |
Prince, M., Sivanandan, R., Kaczorowski, A., Wolf, G., Kaplan, M., Dalerba, P., Weissman, I., Clarke, M. and Ailles, L.: Identification of a Subpopulation of Cells with Cancer Stem Cell Properties in Head and Neck Squamous Cell Carcinoma., Proc Natl Acad Sci U S A 104,
973, 2007 |
Mouse
|
Mouse |
Liver epithelial progenitor cells |
Collagenase Type 4: 0.1%
Deoxyribonuclease I: 0.05%
|
DMEM |
Li Wen-Lin, Su Juan, Yao Yu-Cheng, Tao Xin-Rong, Yan Yong-Bi, Yu Hong-Yu, Wang Xin-Min, Li Jian-Xiu, Yang Yong-Ji, Lau Joseph T Y, Hu Yi-Ping: Isolation and characterization of bipotent liver progenitor cells from adult mouse, Stem Cells 24,
322-32, 2006 |
Mouse
|
Mouse, 6 week |
Adipose mesenchymal stem |
Collagenase: 0.2%
|
PBS |
Di Rocco Giuliana, Iachininoto Maria Grazia, Tritarelli Alessandra, Straino Stefania, Zacheo Antonella, Germani Antonia, Crea Filippo, Capogrossi Maurizio C: Myogenic potential of adipose-tissue-derived cells, J Cell Sci
119,
2945-52, 2006 |
Mouse
|
Mouse |
Neural stem cells |
Papain: see reference
|
DMEM/F-12 |
Meletis, K., Wirta, V., Hede, S., Nister, M., Lundeberg, J. abd Frisen, J.: p53 Suppresses the Self-Renewal of Adult Neural Stem Cells., Development
133,
363, 2006 |
Mouse
|
Mouse, 10-12 week |
Kidney |
Collagenase Type 4: 0.075%
|
DMEM |
Dekel, B., Zangi, L., Shezen, E., Reich-Zeliger, S., Eventov-Friedman, S., Katchman, H., Jacob-Hirsch, J., Amariglio, N., Rechavi, G., Margalit, R. and Reisner, Y.: Isolation and Characterization of Nontubular sca-1+lin- Multipotent Stem/Progenitor Cells from Adult Mouse Kidney., J Am Soc Nephrol
17,
3300, 2006 |
Mouse
|
Mouse, day 7 |
Cerebellar stem cells |
Papain: 10u/ml
Deoxyribonuclease I: 250 u/ml
|
Dulbecco's PBS |
Lee, A, Kessler, J, Read, T, Kaiser, C, Corbeil, D, Huttner ,W, Johnson, J, Wechsler-Reya, R.: Isolation of Neural Stem Cells from the Postnatal Cerebellum, Nat Neurosci 8,
723, 2005 |
Mouse
|
Mouse, embryonic |
Embryonic fibroblast feeder cells |
Collagenase Type 4: 0.1%
|
DMEM |
Schatten Gerald, Smith Joseph, Navara Christopher, Park Jong-Hyuk, Pedersen Roger: Culture of human embryonic stem cells, Nat Methods
2,
455-63, 2005 |
Mouse
|
Mouse, 1 day |
Neural progenitor |
PDS kit: per instructions
|
see reference |
Seaberg, R., Smukler, S. and Van der Kooy, D.: Intrinsic Differences Distinguish Transiently Neurogenic Progenitors from Neural Stem Cells in the Early Postnatal Brain., Dev Biol 278,
71, 2005 |
Mouse
|
Mouse, 8-10 week |
Liver derived stem cells |
Collagenase Type 1: 0.1%
Neutral Protease: 2.4 u/ml
|
HBSS |
Kotton, D., Fabian, A. and Mulligan, R.: A Novel Stem-Cell Population in Adult Liver with Potent Hematopoietic-Reconstitution Activity., Blood 106,
1574, 2005 |
Mouse
|
Mouse |
Neurosperes |
PDS kit: per instructions
|
DMEM/F12 |
Klein C, Butt SJ, Machold RP, Johnson JE, and Fishell G.: Cerebellum- and forebrain-derived stem cells possess intrinsic regional character, Development
132,
4497, 2005 |
Mouse
|
Mouse |
Bone marrow |
Collagenase Type 1: 0.15%
Neutral Protease: 0.15%
|
PBS |
Bertoncello, I. and Williams, B.: Hematopoietic Stem Cell Characterization by Hoechst 33342 and Rhodamine 123 Staining., Methods Mol Biol 263,
181, 2004 |
Mouse
|
Mouse, 4-7 day |
Pluripotent stem cells |
Collagenase Type 1: 220 u/ml
Neutral Protease: 33 u/ml
|
MEM |
Howell, J., Lee, W., Morrison, P., Zhong, J., Yoder, M. and Srour, E.: Pluripotent Stem Cells Identified in Multiple Murine Tissues., Ann N Y Acad Sci 996,
158, 2003 |
Mouse
|
Mouse, 15 day or 3 month |
Neural stem cells |
Papain: 0.1%
|
DMEM/F-12 |
Gritti, A, Bonfanti, L, Doetsch, F, Caille, I, Alvarez-Buylla, A, Lim, D, Galli, R, Verdugo J, Herrera, D, and Vescovi A.: Multipotent Neural Stem Cells Reside into the Rostral Extension and Olfactory Bulb of Adult Rodents, J Neurosci 22(2),
437, 2002 |
Mouse
|
Mouse, embryonic |
Cortical progenitors |
PDS kit: per instructions
|
Serum free medium |
Estivill-Torrus, G., Pearson, H., Van Heyningen, V., Price, D. and Rashbass, P.: Pax6 is Required to Regulate the Cell Cycle and the Rate of Progression from Symmetrical to Asymmetrical Division in Mammalian Cortical Progenitors., Development
129,
455, 2002 |
Mouse
|
Mouse, 6-8 week |
Myocytes |
Collagenase Type 2: 0.2%
Trypsin: 0.25%
|
HBSS |
McKinney-Freeman SL, Jackson KA, Camargo FD, Ferrari G, Mavilio F, Goodell MA.: Muscle-derived hematopoietic stem cells are hematopoietic in origin, Proc Natl Acad Sci U S A 99,
1341, 2002 |
Mouse
|
Mouse |
Mammary epithelial stem |
Collagenase Type 3: 0.1%
Hyaluronidase: 0.1%
Pronase: 1.25%
Deoxyribonuclease I: 0.2%
|
DMEM |
Boulanger CA, Smith GH: Reducing mammary cancer risk through premature stem cell senescence, Oncogene
20,
2264-72, 2001 |
Mouse
|
Mouse, male |
Prostatic stem |
Collagenase Type 1: 170 u/ml
|
DMEM |
Dubey P, Wu H, Reiter RE, Witte ON: Alternative pathways to prostate carcinoma activate prostate stem cell antigen expression, Cancer Res 61,
3256-61, 2001 |
Mouse
|
Mouse, 4-8 month |
Neural subventricular zone |
Trypsin: 0.13%
Hyaluronidase: 0.067%
|
DMEM/F12 |
Gritti A, Frolichsthal-Schoeller P, Galli R, Parati E, Cova L, Pagano S, Bjornson C, and Vescovi A.: Epidermal and Fibroblast Growth Factors Behave as Mitogenic Regulators for a Single Multipotent Stem Cell-like Population from the Subventricular Region of the Adult Mouse forebrain, J Neurosci 19(9),
3287, 1999 |
Mouse
|
Mouse, 6 week |
Muscle hematopoietic stem cells |
Collagenase: 0.2%
Trypsin: 0.1%
|
DMEM |
Jackson, K., and Goodell, M.: Hematopoietic Potential of Stem Cells Isolated from Murine Skeletal Muscle, Proc Natl Acad Sci U S A 96,
14482, 1999 |
Ovine
|
Ovine, 3-5 year |
Endometrial stromal |
Collagenase Type 1: 0.5%
Deoxyribonuclease I: 0.04 u/ml
|
DMEM/F-12 |
Letouzey, V., Tan, K., Deane, J., Ulrich, D., Gurung, S., Ong, Y. and Gargett, C.: Isolation and Characterisation of Mesenchymal Stem/Stromal Cells in the Ovine Endometrium., PLoS ONE
10,
e0127531, 2015 |
Porcine
|
Porcine, 3-4 month |
Synovial membrane and skin stem |
Collagenase: 0.2%
|
DMEM |
Ando, W., Kutcher, J., Krawetz, R., Sen, A., Nakamura, N., Frank, C. and Hart, D.: Clonal Analysis of Synovial Fluid Stem Cells to Characterize and Identify Stable Mesenchymal Stromal Cell/Mesenchymal Progenitor Cell Phenotypes in a Porcine Model: A Cell Source with Enhanced Commitment to the Chondrogenic Lineage., Cytotherapy
16,
776, 2014 |
Porcine
|
Porcine, 1-4 day |
Testicular |
Collagenase Type 4: 0.1%
Hyaluronidase: 0.1%
Trypsin: 0.25%
|
DMEM |
Park, M., Park, J., Kim, M., Lee, K., Park, H., Yun, J., Choi, J., Lee, E. and Lee, S.: Development of a High-Yield Technique to Isolate Spermatogonial Stem Cells From Porcine Testes., J Assist Reprod Genet
31,
983-91, 2014 |
Porcine
|
Porcine, female, <1 year |
Adipose mesenchymal stem |
Collagenase Type 1: 0.1%
|
DMEM |
Williams, K., Picou, A., Kish, S., Giraldo, A., Godke, R. and Bondioli, K: Isolation and Characterization of Porcine Adipose Tissue-Derived Adult Stem Cells., Cells Tissues Organs
188,
251, 2008 |
Porcine
|
Porcine, male, 8 day |
Seminiferous epithelial cells |
Collagenase: 0.15%
Deoxyribonuclease I: .0001%
Hyaluronidase: 0.15%
Trypsin: 0.05%
|
DMEM/F12 |
Dirami G, Ravindranath N, Pursel V, Dym M: Effects of stem cell factor and granulocyte macrophage-colony stimulating factor on survival of porcine type A spermatogonia cultured in KSOM, Biol Reprod 61,
225-30, 1999 |
Rabbit
|
Rabbit, New Zealand, 8-10 week |
Tenocytes and tendon stem cells |
Collagenase Type 1: 0.3%
Neutral Protease: 0.4%
|
DMEM |
Zhang, J. and Wang, J.: Characterization of Differential Properties of Rabbit Tendon Stem Cells and Tenocytes., BMC Musculoskelet Disord
11,
10, 2010 |
Rat
|
Rat, SD, 2-3 month |
Mesenchymal stromal |
Collagenase Type 1: 0.1%
|
DMEM |
Dayer, D., Tabar, M., Moghimipour, E., Tabandeh, M., Ghadiri, A., Bakhshi, E., Orazizadeh, M. and Ghafari, M.: Sonic Hedgehog Pathway Suppression and Reactivation Accelerates Differentiation of Rat Adipose-Derived Mesenchymal Stromal Cells Toward Insulin-Producing Cells, Cytotherapy
19,
937-946, 2017 |
Rat
|
Rat |
Placental mesenchymal |
Collagenase Type 4: 0.1%
Deoxyribonuclease I: 0.02%
Neutral Protease: 0.1%
|
DMEM |
Jiang, H., Zhang, Y., Tian, K., Wang, B. and Han, S.: Amelioration of Experimental Autoimmune Encephalomyelitis Through Transplantation of Placental Derived Mesenchymal Stem Cells., Sci Rep
7,
41837, 2017 |
Rat
|
Rat, 9 week |
Adipose stromal |
Collagenase: 0.1%
|
DMEM |
Ohta, Y., Hamaguchi, A., Ootaki, M., Watanabe, M., Takeba, Y., Iiri, T., Matsumoto, N. and Takenaga, M.: Intravenous Infusion of Adipose-Derived Stem/Stromal Cells Improves Functional Recovery of Rats with Spinal Cord Injury., Cytotherapy
19,
839-848, 2017 |
Rat
|
Rat |
Mesenchymal stem |
Collagenase Type 1: 0.075%
|
DMEM |
Emre, E, Yuksel, N, Duruksu, G, Pirhan, D, Subasi, C, Erman, G and Karaoz, E: Neuroprotective Effects of Intravitreally Transplanted Adipose Tissue and Bone Marrow-Derived Mesenchymal Stem Cells in an Experimental Ocular Hypertension Model., Cytotherapy
17,
543-59, 2015 |
Rat
|
Rat. SD, 5 month |
Mesenchymal stromal |
Collagenase Type 2: 0.075%
|
DMEM |
Veronesi, F, Torricelli, P, Della, B, Pagani, S and Fini, M: In Vitro Mutual Interaction Between Tenocytes and Adipose-Derived Mesenchymal Stromal Cells., Cytotherapy
17,
215-23, 2015 |
Rat
|
Rat, 6-8 week |
Spinal cord neural progenitor |
PDS kit: with modifications
|
EBSS |
Mothe, A. and Tator, C.: Isolation of Neural Stem/Progenitor Cells from the Periventricular Region of the Adult Rat and Human Spinal Cord., J Vis Exp
,
e52732, 2015 |
Rat
|
Rat, adult |
Tumor |
Collagenase Type 1: 0.16%
Hyaluronidase: 0.002%
Deoxyribonuclease I: 0.006%
|
DMEM/F12 |
Duarte, S., Momier, D., Baque, P., Casanova, V., Loubat, A., Samson, M., Guigonis, J., Staccini, P., Saint-Paul, M., De Lima, M., Carle, G. and Pierrefite-Carle, V.: Preventive Cancer Stem Cell-Based Vaccination Reduces Liver Metastasis Development in a Rat Colon Carcinoma Syngeneic Model., Stem Cells 31,
423-32, 2013 |
Rat
|
Rat, SD, neonatal |
Brown adipocytes |
Collagenase Type 4: 0.1%
Neutral Protease: 0.1%
Trypsin: 0.05%
|
PBS |
Liu, Z., Wang, H., Zhang, Y., Zhou, J., Lin, Q., Wang, Y., Duan, C., Wu, K. and Wang, C.: Efficient Isolation of Cardiac Stem Cells from Brown Adipose., J Biomed Biotechnol
2010,
104296, 2010 |
Rat
|
Rat |
Adipose derived stem |
Collagenase Type 1: 0.1%
|
DMEM |
Wei, X., Du, Z., Zhao, L., Feng, D., Wei, G., He, Y., Tan, J., Lee, W., Hampel, H., Dodel, R., Johnstone, B., March, K., Farlow, M. and Du, Y.: IFATS Collection: The Conditioned Media of Adipose Stromal Cells Protect Against Hypoxia-Ischemia-Induced Brain Damage In Neonatal Rats., Stem Cells 27,
478, 2009 |
Rat
|
Rat, SD, newborn |
Neutral stem cells, Schwann cells |
Trypsin: 0.25%
Collagenase: 0.16%
|
DMEM/F12 |
Zeng Yuan-Shan, Ding Ying, Wu Li-Zhi, Guo Jia-Song, Li Hai-Biao, Wong Wai-Man, Wu Wu-Tian: Co-transplantation of schwann cells promotes the survival and differentiation of neural stem cells transplanted into the injured spinal cord, Dev Neurosci 27,
20-6, 2005 |
Rat
|
Rat, 2 month |
Spinal cord progenitor cells
Hybrid toxins |
PDS kit: see reference
|
Neurobasal A |
Mothe, A., Kulbatski, I., Van Bendegem, R., Lee, L., Kobayashi, E., Keating, A., and Tator, C.: Analysis of Green Fluorescent Protein Expression in Transgenic Rats for Tracking Transplanted Neural Stem/Progenitor Cells, J Histochem Cytochem 53(10),
1215, 2005 |
Rat
|
Rat, male, 200-250 g |
Neural stem cells |
Papain: 0.09%
Deoxyribonuclease I: 0.1%
|
EBSS |
Gobbel GT, Choi SJ, Beier S, Niranjan A: Long-term cultivation of multipotential neural stem cells from adult rat subependyma, Brain Res 980,
221, 2003 |
Rat
|
Rat, postnatal, adult |
Gut neural crest stem |
Collagenase Type 4: 0.1%
Trypsin: 0.025%
|
HBSS |
Kruger, G., Mosher, J., Bixby, S., Joseph, N., Iwashita, T. and Morrison, S.: Neural Crest Stem Cells Persist in the Adult Gut but Undergo Changes in Self-Renewal, Neuronal Subtype Potential, and Factor Responsiveness., Neuron 35,
657, 2002 |
Rat
|
Rat, SD, embyronic |
Sciatic nerve and gut neural crest stem |
Collagenase Type 4: 0.025%
Trypsin: 0.005%
Deoxyribonuclease I: 0.05%
|
HBSS |
Bixby, S., Kruger, G., Mosher, J., Joseph, N. and Morrison, S.: Cell-Intrinsic Differences Between Stem Cells from Different Regions of the Peripheral Nervous System Regulate the Generation of Neural Diversity., Neuron 35,
643, 2002 |
Rat
|
Rat, adult |
Neural stem cells |
Papain: 2.5 u/ml
Deoxyribonuclease I: 250 u/ml
Neutral Protease: 1 u/ml
|
DMEM/F-12 |
Palmer, T., Markakis, E., Willhoite, A., Safar, F., and Gage, F.: Fibroblast Growth Factor-2 Activates a Latent Neurogenic Program in Neural Stem Cells from Diverse Regions of the Adult CNS, J Neurosci 19,
8487, 1999 |
Rat
|
Rat |
Central nervous system stem cells |
Papain: 0.01%
Neutral Protease: 0.1%
|
DMEM/F-12 |
Ray, J. and Gage, F.: Neural Stem Cell Isolation, Characterization and Transplantation,Modern Techniques in Neuroscience Research,Johansson, H. and Windhorst, U.,
Springer-Verlag New York,
339, 1999 |
Rat
|
Rat, SD |
Sciatic nerves |
Trypsin: 0.025%
Collagenase Type 3: 0.1%
|
L-15 medium (see reference) |
Morrison, S., White, P., Zock, C., and Anderson, D.: Prospective Identification, Isolation by Flow Cytometry, and In Vivo Self-Renewal of Multipotent Mammalian Neural Crest Stem Cells, Cell
96,
737, 1999 |
Rat
|
Rat (also mice) |
Spinal cord |
Trypsin: 0.133%
|
HBSS and PIPES |
Johansson, C., Momma, S., Clarke, D., Risling, M., Lendahl, U., and Frisen, J: Identification of a Neural Stem Cell in the Adult Mammalian Central Nervous System, Cell
96,
25, 1999 |
Rat
|
Rat, embryonic |
Neurons and progenitor |
Papain: 20 u/ml
|
EBSS |
Maric, D., Maric, I. and Barker, J.: Buoyant Density Gradient Fractionation and Flow Cytometric Analysis of Embryonic Rat Cortical Neurons and Progenitor Cells., Methods
16,
247, 1998 |
Rat
|
Rat |
Stem, neural crest |
Collagenase: 0.075%
|
Ringer's solution |
Stemple, D., and Anderson, D.: Isolation of a Stem Cell for Neurons and Glia from the Mammalian Neural Crest, Cell
71,
973, 1992 |
|
|