Thymus - Tissue Tables
Species | Species Detail | Cells | Enzyme(s) | Medium |
Reference |
Human
|
Human |
Dentritic |
Collagenase Type 2: 0.1%
Deoxyribonuclease I: 0.002%
|
RPMI 1640 |
Vandenabeele S, Hochrein H, Mavaddat N, Winkel K, Shortman K: Human thymus contains 2 distinct dendritic cell populations, Blood 97,
1733-41, 2001 |
Mouse
|
Mouse |
Thymic stromal |
Collagenase Type 4: 0.02%
Neutral Protease: 0.02%
Deoxyribonuclease I: 0.0025%
|
RPMI-1640 |
Rode, I. and Boehm, T.: Regenerative Capacity of Adult Cortical Thymic Epithelial Cells., Proc Natl Acad Sci U S A 109,
3463-8, 2012 |
Mouse
|
Mouse |
Stromal |
Collagenase Type 3: 0.2%
Hyaluronidase: 0.1%
|
RPMI 1640 |
Phillips, Joy, Brondstetter, T., English, C., Lee, H., Virts, E, and Thoman, M.: IL-7 Gene Therapy in Aging Restores Early thymopoiesis without Reversing Involution, J Immunol ,
4869, 2004 |
Mouse
|
Mouse |
Dentritic |
Collagenase Type 3: 0.1%
Deoxyribonuclease I: 325 u/ml
|
RPMI 1640 |
Schiavoni, F, Mattei, F, Sestili, P, Borghi, P, Venditti, M, Morse, H, Belardelli, F, and Gabrieli, L: ICSBP is Essential for the Development of Mouse Type I Interferon-producing Cells and for the Generation and Activation of CD8a+ Dendritic Cells, J Exp Med 196,
1415, 2002 |
Mouse
|
Mouse, 3-6 week |
Thymic |
Collagenase Type 3: 100-400 u/ml
|
HBSS |
Smith KM, Olson DC, Hirose R, Hanahan D: Pancreatic gene expression in rare cells of thymic medulla: evidence for functional contribution to T cell tolerance, Int Immunol 9,
1355-65, 1997 |
Mouse
|
Mouse (BALB/c, C3H, C57BL/6), 1-28 day |
Epithelial, thymus |
Neutral Protease: 1.5 µg/ml
|
DMEM |
Ropke, C., van Deurs, B., and Petersen, O.: Short-term Cultivation of Murine Thymic Epithelial Cells in a Serum-Free Medium, In Vitro Cell Dev Biol 26,
671, 1990 |
Mouse
|
Mouse, C3H, 16 wk old, female |
Epithelial |
Collagenase Type 3: 0.1%
|
DMEM |
Ehmann, U., Shiurba, R., and Peterson, W.: Long-Term Proliferation of Mouse Thymic Epithelial Cells in Culture, In Vitro Cell Dev Biol 22 (12),
738, 1986 |
Mouse
|
Mouse, Swiss, 6 wk |
Thymus |
Collagenase Type 3: 150 u/ml
|
DMEM |
Jones, K. and Pierre, R.: Analysis of Cellular Heterogeneity in Mouse Thymus Cultures, In Vitro 17,
431, 1981 |
Rat
|
Rat, postnatal |
Thymic |
Trypsin: 0.05%
|
HBSS |
Bonfanti, P., Claudinot, S., Amici, A., Farley, A., Blackburn, C. and Barrandon, Y.: Microenvironmental Reprogramming of Thymic Epithelial Cells to Skin Multipotent Stem Cells., Nature
466,
978, 2010 |
Rat
|
Rat |
Thymic epithelial |
Collagenase Type 3: 0.1%
|
DMEM |
Masuda, A and Matsuyama, M: Epithelial Cell Lines From Rat Thymoma and Rat Thymus,Cell & Tissue Culture: Laboratory Procedures Vol. 2,Doyle, A., Griffiths, J., and Newell, D.,
John Wiley and Sons, Ltd.,
21C:4.1, 1995 |
Rat
|
Rat (ACI/NMs X BUF/Mna) F1, male, 28 months Rat, (ACI/MNs) male, 8 weeks (also bovine, adult) |
Epthelial |
Collagenase Type 3: 0.1%
|
Eagle's MEM Serum-free |
Masuda, A., Ohtsuka, K., and Matsuyama, M.: Establishment of Functional Epithelial Cell Lines from a Rat Thyoma and a Rat Thymus, In Vitro Cell Dev Biol 26,
713, 1990 |
|
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