Tissue Dissociation References | |
Tissue Type: Uterus |
Uterus: Mouse | |
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Bigsby, R., Cooke, P., and Cunha, G.: A Simple Efficient Method For Separating Murine Uterine Epithelial and Mesenchymal Cells, Am J Physiol 251, E630, 1986 | |
Cells: | Epithelial cellsMesencymal cells |
Enzyme(s) | Trypsin: 1% Soybean Trypsin Inhibitor: 1% Deoxyribonuclease: 0.1% |
Medium | DMEM |
Uterus: Mouse | |
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Cooke, P., Uchima, F., Fujii, D., Bern, H., and Cunha, G.: Restoration of Normal Morphology and Estrogen Responsiveness in Cultured Vaginal and Uterine Epithelia Transplanted with Stroma, Proc Natl Acad Sci U S A 83, 2109, 1986 | |
Cells: | Epithelial cells |
Enzyme(s) | Collagenase Type III: 0.1% Trypsin: 5 mg |
Medium | Medium 199 |
Uterus: Mouse | |
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Tomooka, Y., DiAugustine, R., and McLachlan, J.: Proliferation of Mouse Uterine Epithelial Cells in Vitro, Endocrinology 118 (3), 1011, 1986 | |
Cells: | Epithelial cells |
Enzyme(s) | Trypsin |
Medium | Medium 199 |
Uterus: Rat | |
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Glasser, S., Julian, J., Decker, G., Tang, J., and Carson, D.: Development of Morphological and Functional Polarity in Primary Cultures of Immature Rat Uterine Epithelial Cells, J Cell Biol 107 (6), 2409, 1988 | |
Cells: | Luminal epithelial cells |
Enzyme(s) | Trypsin |
Medium | HBSS |
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