Diaphorase Product Information

Source: Clostridium kluyveri

I.U.B.: 1.6.99.1

Diaphorase catalyzes the reaction of a reduced di- or tri-phosphopyridine nucleotide hydrogen donor with a hydrogen acceptor, usually a dye in the leucoform.

Stability/Storage: Stable for 6-12 months at 2-8°C. Keep lyophilized powder at -20°C for long-term storage. Due to the presence of FMN, the enzyme, especially in solution, is light-sensitive.

Unit Definitions: For Code: DILW, 1 Unit equals a decrease in absorbance at 600nm of 1.0 per minute at 25°C, pH 7.5. For Code: DIL, 1 Unit reduces 1µmole of DCPIP per minute at 25°C, pH 8.5.