Protease, Staph aureus (Endoproteinase Glu-C) Product Information


Source: Staphylococcus aureus V8

I.U.B.: 3.4.21.19

Protease S. aureus V8 (Endoproteinase-Glu-C) specifically cleaves peptide bonds on the COOH-terminal side of either aspartic or glutamic acids. In the presence of ammonium, the enzyme specificity is limited to glutamic sites. It has a molecular weight of 27,000 daltons and optimum pH's of 4.0 and 7.8 with hemoglobin as the substrate. Protease S. aureus V8 is inhibited by diisopropylfluorophosphate and monovalent anions such as F-, Cl-, CH3COO- and NO3. Enzyme activity is determined by the casein digestion assay described by Drapeau (Methods Enzymol., 45, 469, 1976).

Stability/Storage: Autolysis occurs at temperatures greater than 40°C. The enzyme is fully active in USP 0.2% SDS. Stable for 12 months at 2-8°C.

Unit Definition: One Unit causes a change of 0.001 A280 nm per minute at 37°C, pH 7.8 using casein as the substrate.

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DescriptionActivityCodeSizeCat. No.Price
Protease, S. aureus, Sequencing Grade
Chromatographically purified according to Drapeau, G, et.al, JBC, 247, 6720 (1972). Supplied as a lyophilized powder containing 10µg or 50µg protein.
Store at 2-8°C.
N/A STSEQ 5x10 ug LS02126 $174.00 Buy Now
5x50 ug LS02128 $472.00 Buy Now
Bulk LS02129 Inquire
Protease, S. Aureus (Endoproteinase Glu-C)
Chromatographically purified according to Drapeau, G., Boily, Y., and Houmard, J.: JBC, 247, 6720 (1972). A lyophilized powder.
Store at 2-8°C.
≥500 units per mg dry weight STAP 1 mg LS003608 $67.00 Buy Now
5 mg LS003605 $246.00 Buy Now
Bulk LS003606 Inquire

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