Alkaline phosphatase is a broad term associated with non-specific phosphomonoesterases with an alkaline pH optimum.
Source/Unit Definitons: CAP: Calf intestine. One Worthington Unit hydrolyzes 1µmole of p-nitrophenol phosphate per minute at 37°C, pH 9.8.
BAPF, BAPC, BAPSF:E. coli. One Unit hydrolyzes 1µmole of p-nitrophenol phosphate per minute at 25°C, pH 8.0.
PC: Chicken Intestine. One Unit hydrolyzes 1µmole of o-carboxyphenol phosphate per minute at 25°C, pH 8.8.
Technical Notes: Worthington chicken intestine alkaline phosphatase (Code: PC) is the preparation used in the NF/USP dexamethasone phosphate measurement.
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Phosphatase, Alkaline, Purified Source: Calf Intestine Chromatographically purified, EIA grade. A solution in 50% glycerol containing 5mM Tris, 5mM MgCl2 and 0.1mMZnCl2. Protein concentration is approximately 20mg/ml.
Store at 2-8°C.
Phosphatase, Alkaline, Purified Source:E. coli Chromatographically purified from Code: BAPC. Ribonuclease <0.002% by weight as RNase A using a poly C assay. Phosphodiesterase not detectable when assayed at 0.1 mg/ml with p-nitrophenyl thymidine 5' phosphate. A suspension in 2.6M ammonium sulfate, pH 8.0.
Store at 2-8°C.
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