Deoxyribonucleic Acid and Related Products Product Information
Worthington offers DNA purified from these sources:
Calf thymus: (Code: DNA) Prepared and purified by a method developed at Worthington to have lower protein and RNA contamination than most other commercial preparations. This highly polymerized DNA is an excellent substrate for deoxyribonuclease. A sodium salt, it must be converted by adding magnesium ions to be susceptible to DNase.
Calf thymus DNA, covalently bound to cellulose is also available: DNA Cellulose, Double-Stranded (Code: DNACELDS) DNA Cellulose, Single-Stranded (Code: DNACELSS)
Salmon Testes: (Code: SDNA) Prepared by a modification of the method of Emanuel and Chaikoff, J. Biol. Chem., 203, 164, (1953). A minimum of 75% native nucleic acid.
Salmon Testes DNA, Denatured & Fragmented (Code: SDNAD) is also available. Prepared by mechanical shearing and heat denaturation.
Escherichia coli: (Code: DNAEC) Isolated as described by Marmur, J. Mol. Biol., 3, 208, 1961.
Lambda phage DNA (Code: DNAL) is prepared from CsCl purified phage and is purified to an A260/A280 ≥1.8. Homogeneous in agarose gel electrophoresis. A solution in 10 mM Tris-HCl pH 8.0 with 1 mM EDTA.
DNA fragments prepared by restriction endonuclease digestion of purified lambda DNA are available (Codes: DNALBSTE; DNALECOR; DNALHIND). Supplied as solutions in 10 mM Tris-HCl pH 8.0 with 1 mM EDTA.
Technical Note: One A260 unit = 50 µg DNA.
Stability/Storage: DNAL: Storage buffer 10 mM Tris-HCl, pH 8.0 containing 1 mM EDTA. Store at -20°C. Once thawed keep at 2-8°C.
Related Products:
Albumin, Nuclease-Free (BSANF)
Deoxyribonuclease I (DP/D/DCLS/D2/DPFF/DPRF)
Deoxyribonuclease II (HDA/HDAC/HDACS)
E•RASE RNase A/T1 Blend (RCT)
Histones (H, NHL)
Lysozyme (LY/LYSF)
Nuclease, Micrococcal (NFCP)
Nuclease, S1 (SINUC/SINUCL)
Phosphatase, Alkaline (CAP/BAPF/BAPC/BAPSF/PC)
Phosphodiesterase I (VPH)
Phosphodiesterase II (SPH)
Proteinase K (PROK/PROKS)
Reverse Transcriptase, Recombinant HIV (RTHIV)
Ribonuclease A (R/RAF/RASE/RS/RPDF)
Ribonuclease T1, Animal Origin Free (RT1S)
Description
Activity
Code
Size
Cat. No.
Price
Deoxyribonucleic Acid, Calf Thymus Source: Calf Thymus Highly polymerized; hyperchromicity 27%. A substrate for deoxyribonuclease assays. Prepared by a method developed at Worthington to remove contaminating RNA and protein. Supplied dried.
Store at 2-8°C.
DNA Cellulose, Double-Stranded Source: Calf Thymus Prepared by a method developed at Worthington in which native, double-stranded calf thymus DNA is covalently bound to cellulose. Suitable for the purification of many DNA binding proteins such as polymerases, transcription factors, and terminators, etc. Supplied as a dry powder. One gram of DNA-cellulose will swell to 3-4ml when fully hydrated.
Store at 2-8°C.
DNA Cellulose, Single-Stranded Source: Calf Thymus Prepared by a method developed at Worthington in which denatured, single-stranded calf thymus DNA is covalently bound to cellulose. Suitable for the purification of many proteins that are associated with nucleic acids such as DNA/RNA polymerases and endo- and exo-nucleases and reverse transcriptases. Supplied as a dry powder. One gram of DNA-cellulose will swell to 3-4ml when fully hydrated.
Store at 2-8°C.
Deoxyribonucleic Acid, Salmon Testes Source: Salmon Testes Prepared by a modification to the method of Emanuel, C.F., and Chaikoff, I.L., : JBC, 203, 164 (1953). 75% native nucleic acid. Supplied dried.
Store at 2-8°C.
Deoxyribonucleic Acid, Denatured, Fragmented Source: Salmon Testes Prepared from purified salmon testes DNA (Code: SDNA) by mechanical shearing and heat denaturation to an average fragment size of 200-1000 base pairs. To reverse any renaturation occurring during storage this material should be briefly boiled and rapidly chilled before use. Recommended concentration for use is 100µg/ml. A solution at 5 mg/ml in 0.05M NaCl.
Store at -20°C.
REQUIRES SPECIAL SHIPPING: ICE PACK
Deoxyribonucleic Acid, Lambda Source:E. coli Purified to an A260/A280 1.8 from purified phage. Homogeneous by agarose gel electrophoresis. Generates the characteristic five and eight bands after digestion with EcoR I and Hind III respectively. A solution in 10mM Tris-HCl, pH 8.0, with 1mM EDTA.
Store at -20°C.
REQUIRES SPECIAL SHIPPING: DRY ICE
Deoxyribonucleic Acid, Lambda, Hind III Fragments Source: Lambda DNA DNA fragments prepared by the digestion of purified lambda DNA with the restriction endonuclease Hind III. On agarose gel electrophoresis the mixture separates into eight individual bands having the following number of base pairs: 23130, 9416, 6557, 4361, 2322, 2027, 564, and 125. (Note: A higher sample load may be required to clearly see the 564 and 125 base pair bands.) A solution in 10mM Tris-HCl, pH 8.0, with 1mM EDTA.
Store at -20°C.
REQUIRES SPECIAL SHIPPING: DRY ICE
Deoxyribonucleic Acid, Lambda, EcoR I Fragments Source: Lambda DNA DNA fragments prepared by the digestion of purified lambda DNA with the restriction endonuclease EcoR I. On agarose gel electrophoresis the mixture separates into five individual bands having the following number of base pairs: 21226, 7421, 5804, 4878, and 3530. A solution in 10mM Tris-HCl, pH 8.0, with 1mM EDTA.
Store at -20°C.
REQUIRES SPECIAL SHIPPING: DRY ICE
Deoxyribonucleic Acid, Lambda, BstE II Fragments Source: Lambda DNA DNA fragments prepared by the digestion of lambda DNA with the restriction endonuclease BstE II. On agarose gel electrophoresis the mixture separates into 14 individual bands having the following number of base pairs: 8454, 7242, 6369, 5686, 4822, 4324, 3675, 2323, 1929, 1371, 1264, 702, 224 and 117. A solution in 10mM Tris-HCl, pH 8.0, with 1mM EDTA.
Store at -20°C.
REQUIRES SPECIAL SHIPPING: DRY ICE
Deoxyribonucleic Acid, E. coli Source:E. coli Supplied as a dried powder purified from E. coli Type B cells (ATCC#11303) as described by Marmur, J. Mol. Biol., 3, 208 (1961).
Store at 2-8°C.
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