Worthington Collagenase Products, Specifications and Applications Table

Product CodeCollagenaseCaseinaseClostripain TrypticComments/Applications*
CDU/mgdwu/mgdwu/mgdwu/mgdw

Partially Purified

CLS-1≥ 125≥ 200 ≤ 4.0≤ 0.5 Balanced activities/Adipose, Adrenal, Epithelial, Liver, Lung
CLS-2≥ 125≥ 200 ≥ 3.5≥ 0.1 Higher proteolytic activities/Bone, Heart, Liver, Thymus
CLS-3≥ 100≥ 50 ≤ 3.0≤ 0.3 Lower proteolytic activities/Mammary
CLS-4≥ 160≥ 100 ≤ 3.0≤ 0.1 Lower tryptic activity/Pancreatic Islets
CLS-5≥ 450≥ 450 ≤ 3.0≤ 0.3 Higher collagenase and caseinase activities
CLS-6 New!≥ 400≥ 1,000 ≤ 4.0≤ 0.5 Higher activity with caseinase to collagenase ratio ~2:1, designated to be enriched for Type II (coli> H) collagenase relative to Type I (coli> G)
CLS-7 New!≥ 1,000≥ 2,000 ≤ 8.0≤ 0.5 Contains collagenase and caseinase activities 4X higher than collagenase types 1 & 2
CLSS-1≥ 125≥ 200 ≤ 4.0≤ 0.5 0.22µ Filtered CLS-1 in 50mg & 1 gm Vials
CLSS-2≥ 125≥ 200 ≥ 3.5≥ 0.1 0.22µ Filtered CLS-2 in 50mg & 1 gm Vials
CLSS-3≥ 100≥ 50 ≤ 3.0≤ 0.3 0.22µ Filtered CLS-3 in 50mg Vials
CLSS-4≥ 160≥ 100 ≤ 3.0≤ 0.1 0.22µ Filtered CLS-4 in 50mg & 1 gm Vials
CLSS-5≥ 450≥ 100 ≤ 3.0≤ 0.3 0.22µ Filtered CLS-5 in 50mg & 1 gm Vials
CLSH≥ 125≥ 200 ≤ 4.0≤ 0.5 0.22µ Filtered, ≥ 22,500U CLS-1 & 30U ESL component of HIS kit
 

Animal Origin Free

CLSAFA≥ 150≥ 150 ≤ 8.0≥ 0.1 Balanced activities/AOF Stem Cell & Tissue Bioprocessing
CLSAFB≥ 300≥ 300 ≤ 5.0≤ 0.5 Higher activities/AOF Stem Cell & Tissue Bioprocessing
CLSAFC≥ 200≥ 150 ≤ 3.0≤ 0.1 Lower Protease Activities/AOF Stem Cell & Tissue Bioprocessing
 
CLSAFAS≥ 150≥ 150 ≤ 8.0≥ 0.1 0.22µ Filtered AOF CLSAFA in 50mg Vials
CLSAFBS≥ 300≥ 300 ≤ 5.0≤ 0.5 0.22µ Filtered AOF CLSAFB in 50mg Vials
CLSAFCS≥ 200≥ 150 ≤ 3.0≤ 0.1 0.22µ Filtered AOF CLSAFC in 50mg Vials

STEMxyme® Animal Origin Free Blends

STZ1≥ 250≥ 1,000 ≤ 5.0≤ 0.5 0.22µ Filtered CLSAFB & NPRO/AOF Stem Cell & Tissue Bioprocessing
STZ2≥ 250≥ 2,000 ≤ 5.0≤ 0.5 0.22µ Filtered CLSAFB & NPRO/AOF Stem Cell & Tissue Bioprocessing

Chromatographically Purified

CLSPA≥ 500≤ 50 ≤ 2.0≤ 0.25 Low Protease/Collagen Studies, Tissue Digestion combined with other proteases
CLSPANK≥ 500≤ 50 ≤ 2.0≤ 0.25 0.22µ Filtered, ≥ 1,500U CLSPA component of NCIS kit

*Correlations between type and effectiveness with different types have been good, but not perfect, and may be dependent partly on parameters of use and objectives as well as lot-to-lot variations. For more information see the Tissue Dissociation Guide and Collagenase Sampling Program information.