Neutral Protease (Dispase) References

• : Keratinocyte Culture, Basic Cell Culture Protocols,(Pollard, J., and Walker, J., Eds.), Humana Press, 117, 1997
• Akimoto, Y., Obinata, A., Endo, H., and Hirano, H.: Immunohistochemical Study of Basement Membrane Reconstruction by an Epidermis-Dermis Recombination Experiment Using Cultured Chick Embryonic Skin: Induction of Tenascin , J Histochem Cytochem 40, 1129, 1992
• Alvarez, V., von der Weid, I., Seldin, L., and Santos, A.: Influence of Growth Conditions on the Production of Extracellular Proteolytic Enzymes in Paenibacillus peoriae NRRL BD-62 and Paenibacillus polymyxa SCE2. , Lett Appl Microbiol 43, 625-30, 2006
• Ash, C., Farrow, J., Wallbanks, S., and Collins, M.: Phylogenetic Heterogeneity of the Genus Bacillus Revealed by Comparative Analysis of Small Subunit-Ribosomal RNA Sequences, Lett Appl Microbiol 13, 202, 1991
• Ash, C., Priest, F., and Collins, M.: Molecular Identification of rRNA Group 3 Bacilli (Ash, Farrow, Wallbanks and Collins) Using a PCR Probe Test. Proposal for the Creation of a New Genus Paenibacillus , Antonie Van Leeuwenhoek 64, 253,
• De Buysscher, E., Kennedy, J., and Mendicino, J.: Synthesis of Mucin Glycoproteins by Epithelial Cells Isolated from Swine Trachea by Specific Proteolysis , In Vitro 20, 433, 1984
• Eijsink, V., Van Den Burg, B., and Venema, G.: High Performance Affinity Chromatography of Bacillus Neutral Proteases , Biotechnol Appl Biochem 14, 275-83, 1991
• Feder, J., and Schuck, J.: Studies on the Bacillus subtilis Neutral Protease and Bacillus thermoproteolyticus Thermolysin-Catalyzed Hydrolysis of Dipeptide Substrates , Biochemistry 9, 2784, 1970
• Feder, J., Keay, L., Garrett, L., Cirulis, N., Moseley, M., and Wildi, B.: Bacillus cereus Neutral Protease. , Biochim Biophys Acta 251, 74, 1971
• Fogarty, W., and Griffin, P.: Production and Purification of the Metalloprotease of Bacillus polymyxa , Appl Microbiol 26, 185, 1973
• Fukumoto, J., and Negoro, H.: Crystallization of Bacterial Proteinase, Proc. Japan Acad. Vol. 27,, , 441, 1951
• Garcia, B., Margolles, E., Roca, H., Mateu, D., Raices, M., Gonzales, M., Herrera, L., and Delgado, J.: Cloning and Sequencing of a Dextranase-Encoding cDNA from Penicillium minioluteum , FEMS Microbiol Lett 143, 175, 1996
• Geggel, H., and Gipson, I.: Removal of Viable Sheets of Conjunctival Epithelium with Dispase II , Inv Ophthal Visual Sci 26, 15, 1985
• Green, H., Kehinde, O., and Thomas, J.: Growth of Cultured Human Epidermal Cells into Multiple Epithelia Suitable for Grafting , Proc Natl Acad Sci U S A 76, 5665, 1979
• Griffin, P., and Fogarty, W.: Some Properties of a Protease from Bacillus polymyxa , Biochem J 125, 109P, 1971
• Griffin, P., Fogarty, W.: Physiochemical Properties of the Native, Zinc- and Manganese-Prepared Metalloprotease of Bacillus polymyxa , Appl Microbiol 26, 191, 1973
• Guntelberg, A., and Ottesen, M.: Purification of the Proteolytic Enzyme from Bacillus subtilis , C R Trav Lab Carlsberg [Chim] 29, 36, 1954
• Hase, C., and Finkelstein, R.: Bacterial Extracellular Zinc-Containing Metalloproteases , Microbiol Rev 57, 823, 1993
• Hirokawa,K., Shimoji, K., and Kajiyama, N.: An Enzymatic Method for the Determination of HemoglobinA(1C) , Biotechnol Lett 27, 963, 2005
• Keay, L., and Wildi, B.: Proteases of the Genus Bacillus . I. Neutral Proteases. , Biotechnol Bioeng 12, 179, 1970
• Kitano, Y., and Okada, N.: Separation of the Epidermal Sheet by Dispase , Br J Dermatol 108, 555, 1983
• Koide, Y., Nakamura, A., Uozumi, T., and Beppu, T.: Cloning and Sequencing of the Major Intracellular Serine Protease Gene of Bacillus subtilis , J Bacteriol 167, 110, 1986
• Kubo, M., and Imanaka, T.: Cloning and Nucleotide Sequence of the Highly Thermostable Neutral Protease Gene from Bacillus stearothermophilus , J Gen Microbiol 134, 1883, 1988
• Kunitz, M.: Crystalline Soybean Trypsin Inhibitor, II. General Properties, J Gen Physiol 30, 291, 1947
• Matsumura T, Yamanaka T, Hashizume S, Irie Y, Nitta K: Tissue Dispersion, Cell Harvest and Fluid Suspension Culture by the Use of Bacterial Neutral Protease , Jpn J Exp Med 45, 377, 1975
• Matsumura, T: Dispase, Cell & Tissue Culture: Laboratory Procedures Vol. 1,(Doyle, A., Griffiths, J., and Newell, D., Eds.), John Wiley and Sons, Ltd., 3B:2.1-2.7, 1995
• Matsumura, T., Nitta, K., Yoshikawa, M., Takaoka, T., and Katsuta, H.: Action of Bacterial Neutral Protease on the Dispersion of Mammalian Cells in Tissue Culture , Jpn J Exp Med 45, 383, 1975
• Matsuoka, H., Okada, M., Ishii, N., and Maeda, S.: Study of the Effects of Dispase in the Chemotherapy of Multicellular Tumour Spheroids of Small-Cell Lung Carcinoma in Man , J Pharm Pharmacol 50, 285, 1998
• McConn, J., Tsuru, D., and Yasunobu, K.: Bacillus subtilis Neutral Proteinase. I. A Zinc Enzyme of High Specific Activity , J Biol Chem 239, 3706, 1964
• Millet, J., Acher, R., and Aubert, J.: Biochemical and Physiological Properties of an Extracellular Protease Produced by Bacillus megaterium , Biotechnol Bioeng 11, 1233, 1969
• Misaki, T., Yamada, M., Okazaki, T., and Sawada, J.: Studies on the Protease Constitution of Aspergillus oryzae , Agric Biol Chem 34, 1383, 1970
• Nagashima, A., Ando, N., Sato, M., Ozawa, S., and Kitajima, M.: Basic Studies on the Application of an Artificial Esophagus Using Cultured Epidermal Cells , Surg Today 27, 915, 1997
• Nagata, M., and Matsumura, T.: Action of the Bacterial Neutral Protease, Dispase, on Cultured Cells and its Application to Fluid Suspension Culture with a Review on Biomedical Application of this Protease , Jpn J Exp Med 56, 297-307, 1986
• Nomoto, M., and Narahashi, Y.: A Proteolytic Enzyme of Streptomyces griseus . IV. General Properties of Streptomyces griseus Protease, J. Biochem. 46, 1645, 1959
• Ono, J., Takaki, R., and Fukuma, M.: Preparation of Single Cells from Pancreatic Islets of Adult Rat by the Use of Dispase. , Endocrinology 24, 265, 1977
• Ottesen, M. and Spector, A.: A Comparison of Two Proteinases from Bacillus subtilis , C R Trav Lab Carlsberg [Chim] 32, 63, 1960
• Roberts G., and Brunt, J.: Identification of an Epidermal Cell-Adhesion Glycoprotein , Biochem J 232, 67, 1985
• Saul, D., Williams, L., Toogood, H., Daniel, R., and Bergquist, P.: Sequence of the Gene Encoding a Highly Thermostable Neutral Proteinase from Bacillus sp. strain EA1: Expression in Escherichia coli and Characterisation , Biochim Biophys Acta 1308, 74, 1996
• Schaefer, B., Wallich, R., Schmolke, K., Fink, W., Bechtel, M., Reinartz, J., and Kramer, M.: Immunohistochemical and Molecular Characterization of Cultured Keratinocytes after Dispase-Mediated Detachment from the Growth Substratum , Exp Dermatol 9, 58, 2000
• Stark, W., Pauptit, R., Wilson, K., and Jansonius, J.: The Structure of Neutral Protease from Bacillus cereus at 0.2-nm resolution. , Eur J Biochem 207, 781-91, 1992
• Stenn, K., Link, R., Moellmann, G., Madri, J., and Kuklinska, E.: Dispase, a Neutral Protease from Bacillus polymyxa , is a Powerful Fibronectinase and Type IV Collagenase , J Invest Dermatol 93, 287, 1989
• Stockle, G., Wahl, R., and Seif, F.: Micromethod of Human Thyrocyte Cultures for Detection of Thyroid-Stimulating Antibodies and Thyrotrophin , Acta Endocrinol (Copenh) 97, 369, 1981
• Takekawa, S., Uozumi, N., Tsukagoshi, N., and Udaka, S.: Proteases Involved in Generation of Beta- and Alpha-Amylases from a Large Amylase Precursor in Bacillus polymyxa , J Bacteriol 173, 6820, 1991
• Tezel, T., Del Priore, L., and Kaplan, H.: Posterior Vitreous Detachment with Dispase , Retina 18, 7, 1998
• Tsuru, D., McConn, J., and Yasunobu, K.: Bacillus subtilis Neutral Proteinase. II. Some Physiochemical Properties , J Biol Chem 240, 2415, 1965
• Van Dorp, A., Verhoeven, M., Nat-Van Der Meij, T., Koerten, H., and Ponec, M.: A Modified Culture System for Epidermal Cells for Grafting Purposes: An in vitro and in vivo Study , Wound Repair Regen 7, 214, 1999
• von der Weid, I., Duarte, G., van Elsas, J., and Seldin, L.: Paenibacillus brasilensis sp. nov.: A Novel Nitrogen-Fixing Species Isolated from the Maize Rhizosphere in Brazil , Int J Syst Evol Microbiol 52, 2147, 2002

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