Worthington Tissue Dissociation Guide

Dissociating Enzymes: Animal Origin Free (AOF) Enzymes

General interest in Animal Origin Free (AOF) tissue dissociation enzymes has dramatically increased to avoid potential contamination with mammalian agents such as prions and viruses. Worthington produces seberal AOF collagenases, proteases and nucleases for those requiring AOF enzymes; please check our current catalog for our these products.

Note: Application specific cell isolation systems have been developed by Worthington to eliminate the need for experimenting with various enzyme combinations and use testing several lots of collagenase. Descriptions for these systems can be found in our current catalog.

 

Next: Dissociating Enzymes: Celase® GMP

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat Adrenal Bone Brain
Cartilage Colon Endothelial Epithelial
Eye Heart Intestine Kidney
Liver Lung Lymph nodes Mammary
Miscellaneous Muscle Neural Pancreas
Parotid Pituitary Prostate Reproductive
Scales Skin Spleen Stem
Thymus Thyroid/Parathyroid Tonsil Tumor

Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.