Worthington Tissue Dissociation GuideMethods and Materials: Basic Primary Cell Isolation(Refer to references for application specific parameters)
Perfusion procedures require special equipment and techniques for recirculating the buffers, media and enzymes. Please refer to referenced texts for additional information and guidance. Next: Equilibration with 95%O2:5%CO2 Tissue Tables (references, grouped by tissue type and species)Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited. |