Worthington Tissue Dissociation GuideDissociating Enzymes: PapainPapain is a sulfhydryl protease from Carica papaya latex. Papain has wide specificity and it will degrade most protein substrates more extensively than the pancreatic proteases. It also exhibits esterase activity. With some tissues papain has proved less damaging and more effective than other proteases. Huettner and Baughman (1986) describe a method using papain to obtain high yields of viable, morphologically intact cortical neurons from postnatal rats. More Information: Worthington Papain Next: Chymotrypsin Tissue Tables (references, grouped by tissue type and species)Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited. |