Worthington Tissue Dissociation Guide

Methods and Materials

Tissue Tables (references, grouped by tissue type and species)

Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.

Table of Contents

1. Introduction
2. Cell Isolation Theory
   • Tissue Types
     • Epithelial Tissue
     • Connective Tissue
   • Dissociating Enzymes
     • Collagenase
     • Trypsin
     • Elastase
     • Hyaluronidase
     • Papain
     • Chymotrypsin
     • Deoxyribonuclease I
     • Neutral Protease (Dispase)
     • Trypsin Inhibitor
     • Animal Origin Free (AOF) Enzymes
     • Celase® GMP
3. Cell Isolation Techniques
   • Methods & Materials
     • Working With Enzymes
     • Basic Primary Cell Isolation
     • Equilibration with 95%O₂:5%CO₂
     • Trituration
     • Enzymatic Cell Harvesting
     • Cell Adhesion and Harvesting
     • Trypsin for Cell Harvesting
     • Cell Release Procedure
   • Optimization Techniques
     • General Guidelines
     • Optimization Strategy
     • Cell Quantitation
     • Measure of Viability
4. Use-Tested Cell Isolation Systems
5. Tissue Culture Glossary
6. Stem Cell Glossary
7. How To Cite Worthington Literature